This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.54 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 76,865 (2.9%) Reads that were too short: 10,695 (0.4%) Reads written (passing filters): 66,170 (2.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 16,588,106 bp (2.1%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 76865 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 324 649.0 0 324 7 1140 649.0 0 1140 8 21981 649.0 0 21981 9 18248 649.0 0 18248 10 53 649.0 0 53 11 13 649.0 0 13 12 22 649.0 0 22 13 17 649.0 0 17 14 13 649.0 0 13 15 18 649.0 0 18 16 12 649.0 0 12 17 11 649.0 0 11 18 12 649.0 0 12 19 24 649.0 0 24 20 9 649.0 0 9 21 7 649.0 0 7 22 16 649.0 0 16 23 5 649.0 0 5 24 6 649.0 0 6 25 16 649.0 0 16 26 13 649.0 0 13 27 10 649.0 0 10 28 11 649.0 0 11 29 13 649.0 0 13 30 13 649.0 0 13 31 10 649.0 0 10 32 14 649.0 0 14 33 8 649.0 0 8 34 10 649.0 0 10 35 8 649.0 0 8 36 12 649.0 0 12 37 27 649.0 0 27 38 14 649.0 0 14 39 8 649.0 0 8 40 25 649.0 0 25 41 15 649.0 0 15 42 34 649.0 0 34 43 6 649.0 0 6 44 23 649.0 0 23 45 32 649.0 0 32 46 31 649.0 0 31 47 86 649.0 0 86 48 18 649.0 0 18 49 227 649.0 0 227 50 42 649.0 0 42 51 12 649.0 0 12 52 12 649.0 0 12 53 18 649.0 0 18 54 18 649.0 0 18 55 19 649.0 0 19 56 17 649.0 0 17 57 10 649.0 0 10 58 38 649.0 0 38 59 32 649.0 0 32 60 46 649.0 0 46 61 40 649.0 0 40 62 27 649.0 0 27 63 14 649.0 0 14 64 13 649.0 0 13 65 10 649.0 0 10 66 268 649.0 0 268 67 83 649.0 0 83 68 158 649.0 0 158 69 11 649.0 0 11 70 17 649.0 0 17 71 29 649.0 0 29 72 12 649.0 0 12 73 25 649.0 0 25 74 12 649.0 0 12 75 8 649.0 0 8 76 12 649.0 0 12 77 14 649.0 0 14 78 11 649.0 0 11 79 15 649.0 0 15 80 16 649.0 0 16 81 16 649.0 0 16 82 35 649.0 0 35 83 46 649.0 0 46 84 91 649.0 0 91 85 75 649.0 0 75 86 33 649.0 0 33 87 21 649.0 0 21 88 47 649.0 0 47 89 24 649.0 0 24 90 21 649.0 0 21 91 373 649.0 0 373 92 71 649.0 0 71 93 60 649.0 0 60 94 59 649.0 0 59 95 27 649.0 0 27 96 12 649.0 0 12 97 29 649.0 0 29 98 20 649.0 0 20 99 18 649.0 0 18 100 31 649.0 0 31 101 20 649.0 0 20 102 36 649.0 0 36 103 73 649.0 0 73 104 88 649.0 0 88 105 44 649.0 0 44 106 37 649.0 0 37 107 55 649.0 0 55 108 36 649.0 0 36 109 49 649.0 0 49 110 65 649.0 0 65 111 150 649.0 0 150 112 122 649.0 0 122 113 287 649.0 0 287 114 118 649.0 0 118 115 407 649.0 0 407 116 694 649.0 0 694 117 5243 649.0 0 5243 118 3682 649.0 0 3682 119 3743 649.0 0 3743 120 40 649.0 0 40 121 25 649.0 0 25 122 28 649.0 0 28 123 15 649.0 0 15 124 31 649.0 0 31 125 45 649.0 0 45 126 46 649.0 0 46 127 47 649.0 0 47 128 33 649.0 0 33 129 37 649.0 0 37 130 47 649.0 0 47 131 68 649.0 0 68 132 65 649.0 0 65 133 93 649.0 0 93 134 37 649.0 0 37 135 33 649.0 0 33 136 28 649.0 0 28 137 40 649.0 0 40 138 72 649.0 0 72 139 46 649.0 0 46 140 66 649.0 0 66 141 33 649.0 0 33 142 33 649.0 0 33 143 22 649.0 0 22 144 43 649.0 0 43 145 67 649.0 0 67 146 99 649.0 0 99 147 58 649.0 0 58 148 64 649.0 0 64 149 64 649.0 0 64 150 62 649.0 0 62 151 643 649.0 0 643 152 692 649.0 0 692 153 586 649.0 0 586 154 234 649.0 0 234 155 64 649.0 0 64 156 47 649.0 0 47 157 29 649.0 0 29 158 28 649.0 0 28 159 39 649.0 0 39 160 45 649.0 0 45 161 53 649.0 0 53 162 160 649.0 0 160 163 106 649.0 0 106 164 99 649.0 0 99 165 37 649.0 0 37 166 51 649.0 0 51 167 35 649.0 0 35 168 24 649.0 0 24 169 43 649.0 0 43 170 61 649.0 0 61 171 56 649.0 0 56 172 39 649.0 0 39 173 44 649.0 0 44 174 29 649.0 0 29 175 29 649.0 0 29 176 26 649.0 0 26 177 39 649.0 0 39 178 42 649.0 0 42 179 104 649.0 0 104 180 132 649.0 0 132 181 107 649.0 0 107 182 126 649.0 0 126 183 135 649.0 0 135 184 93 649.0 0 93 185 214 649.0 0 214 186 25 649.0 0 25 187 24 649.0 0 24 188 24 649.0 0 24 189 45 649.0 0 45 190 61 649.0 0 61 191 79 649.0 0 79 192 58 649.0 0 58 193 31 649.0 0 31 194 24 649.0 0 24 195 35 649.0 0 35 196 31 649.0 0 31 197 49 649.0 0 49 198 48 649.0 0 48 199 50 649.0 0 50 200 34 649.0 0 34 201 42 649.0 0 42 202 37 649.0 0 37 203 48 649.0 0 48 204 48 649.0 0 48 205 41 649.0 0 41 206 35 649.0 0 35 207 35 649.0 0 35 208 43 649.0 0 43 209 31 649.0 0 31 210 62 649.0 0 62 211 30 649.0 0 30 212 84 649.0 0 84 213 55 649.0 0 55 214 71 649.0 0 71 215 56 649.0 0 56 216 76 649.0 0 76 217 247 649.0 0 247 218 266 649.0 0 266 219 361 649.0 0 361 220 256 649.0 0 256 221 90 649.0 0 90 222 40 649.0 0 40 223 101 649.0 0 101 224 76 649.0 0 76 225 99 649.0 0 99 226 232 649.0 0 232 227 86 649.0 0 86 228 209 649.0 0 209 229 131 649.0 0 131 230 53 649.0 0 53 231 280 649.0 0 280 232 157 649.0 0 157 233 252 649.0 0 252 234 229 649.0 0 229 235 130 649.0 0 130 236 109 649.0 0 109 237 91 649.0 0 91 238 102 649.0 0 102 239 100 649.0 0 100 240 69 649.0 0 69 241 27 649.0 0 27 242 64 649.0 0 64 243 58 649.0 0 58 244 79 649.0 0 79 245 208 649.0 0 208 246 197 649.0 0 197 247 286 649.0 0 286 248 145 649.0 0 145 249 75 649.0 0 75 250 136 649.0 0 136 251 114 649.0 0 114 252 124 649.0 0 124 253 170 649.0 0 170 254 148 649.0 0 148 255 177 649.0 0 177 256 82 649.0 0 82 257 96 649.0 0 96 258 50 649.0 0 50 259 67 649.0 0 67 260 42 649.0 0 42 261 56 649.0 0 56 262 27 649.0 0 27 263 57 649.0 0 57 264 64 649.0 0 64 265 78 649.0 0 78 266 101 649.0 0 101 267 77 649.0 0 77 268 102 649.0 0 102 269 38 649.0 0 38 270 76 649.0 0 76 271 84 649.0 0 84 272 93 649.0 0 93 273 109 649.0 0 109 274 68 649.0 0 68 275 57 649.0 0 57 276 47 649.0 0 47 277 77 649.0 0 77 278 136 649.0 0 136 279 157 649.0 0 157 280 151 649.0 0 151 281 95 649.0 0 95 282 103 649.0 0 103 283 96 649.0 0 96 284 108 649.0 0 108 285 112 649.0 0 112 286 115 649.0 0 115 287 105 649.0 0 105 288 105 649.0 0 105 289 79 649.0 0 79 290 53 649.0 0 53 291 138 649.0 0 138 292 108 649.0 0 108 293 198 649.0 0 198 294 107 649.0 0 107 295 76 649.0 0 76 296 95 649.0 0 95 297 78 649.0 0 78 298 101 649.0 0 101 299 121 649.0 0 121 300 120 649.0 0 120 301 64 649.0 0 64 Finished in 3.10 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 66,170 Reads with adapters: 22,802 (34.5%) Reads that were too short: 172 (0.3%) Reads written (passing filters): 22,630 (34.2%) Total basepairs processed: 16,588,106 bp Total written (filtered): 6,177,544 bp (37.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22802 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 18 0.0 3 0 0 15 3 16 204 0.0 3 0 179 16 9 17 21741 0.0 3 20564 992 152 33 18 86 0.0 3 12 63 5 6 19 59 0.0 3 1 0 35 23 20 61 0.0 3 5 2 1 53 23 1 0.0 3 0 0 0 1 27 4 0.0 3 0 0 0 4 30 5 0.0 3 0 0 0 5 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 50 0.0 3 0 0 1 49 42 1 0.0 3 0 0 0 1 43 9 0.0 3 0 0 1 8 46 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 241 0.0 3 0 0 0 241 55 3 0.0 3 0 0 0 3 56 13 0.0 3 0 0 1 12 57 39 0.0 3 0 0 1 38 58 21 0.0 3 0 0 0 21 61 4 0.0 3 0 0 1 3 63 3 0.0 3 0 0 1 2 64 1 0.0 3 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 80 7 0.0 3 0 0 0 7 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 1 1 91 6 0.0 3 0 0 0 6 99 2 0.0 3 0 0 0 2 100 6 0.0 3 0 0 0 6 101 1 0.0 3 0 0 0 1 105 15 0.0 3 0 0 0 15 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 4 0.0 3 0 0 0 4 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 166 38 0.0 3 0 0 2 36 167 8 0.0 3 0 0 0 8 168 2 0.0 3 0 0 0 2 169 41 0.0 3 0 0 0 41 171 3 0.0 3 0 0 0 3 172 2 0.0 3 0 0 0 2 173 3 0.0 3 0 0 0 3 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 220 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 242 2 0.0 3 0 0 1 1 257 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 22,630 Reads with adapters: 310 (1.4%) Reads written (passing filters): 22,630 (100.0%) Total basepairs processed: 6,177,544 bp Total written (filtered): 6,176,591 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 310 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.8% C: 17.7% G: 30.0% T: 6.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 294 353.6 0 294 4 9 88.4 0 9 5 7 22.1 0 7