This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.25 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 67,690 (2.5%) Reads that were too short: 4,476 (0.2%) Reads written (passing filters): 63,214 (2.4%) Total basepairs processed: 800,111,879 bp Total written (filtered): 16,256,913 bp (2.0%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 67690 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 248 649.0 0 248 7 809 649.0 0 809 8 19859 649.0 0 19859 9 22200 649.0 0 22200 10 26 649.0 0 26 11 15 649.0 0 15 12 18 649.0 0 18 13 16 649.0 0 16 14 9 649.0 0 9 15 12 649.0 0 12 16 25 649.0 0 25 17 11 649.0 0 11 18 6 649.0 0 6 19 22 649.0 0 22 20 9 649.0 0 9 21 7 649.0 0 7 22 17 649.0 0 17 23 8 649.0 0 8 24 10 649.0 0 10 25 9 649.0 0 9 26 18 649.0 0 18 27 7 649.0 0 7 28 6 649.0 0 6 29 15 649.0 0 15 30 13 649.0 0 13 31 5 649.0 0 5 32 11 649.0 0 11 33 14 649.0 0 14 34 19 649.0 0 19 35 15 649.0 0 15 36 18 649.0 0 18 37 30 649.0 0 30 38 13 649.0 0 13 39 12 649.0 0 12 40 22 649.0 0 22 41 8 649.0 0 8 42 15 649.0 0 15 43 14 649.0 0 14 44 10 649.0 0 10 45 19 649.0 0 19 46 14 649.0 0 14 47 29 649.0 0 29 48 19 649.0 0 19 49 24 649.0 0 24 50 11 649.0 0 11 51 14 649.0 0 14 52 8 649.0 0 8 53 17 649.0 0 17 54 19 649.0 0 19 55 21 649.0 0 21 56 30 649.0 0 30 57 14 649.0 0 14 58 15 649.0 0 15 59 25 649.0 0 25 60 25 649.0 0 25 61 26 649.0 0 26 62 27 649.0 0 27 63 5 649.0 0 5 64 14 649.0 0 14 65 11 649.0 0 11 66 109 649.0 0 109 67 50 649.0 0 50 68 99 649.0 0 99 69 12 649.0 0 12 70 20 649.0 0 20 71 24 649.0 0 24 72 15 649.0 0 15 73 19 649.0 0 19 74 10 649.0 0 10 75 16 649.0 0 16 76 20 649.0 0 20 77 20 649.0 0 20 78 11 649.0 0 11 79 17 649.0 0 17 80 16 649.0 0 16 81 8 649.0 0 8 82 29 649.0 0 29 83 24 649.0 0 24 84 18 649.0 0 18 85 25 649.0 0 25 86 35 649.0 0 35 87 15 649.0 0 15 88 14 649.0 0 14 89 9 649.0 0 9 90 15 649.0 0 15 91 48 649.0 0 48 92 58 649.0 0 58 93 47 649.0 0 47 94 32 649.0 0 32 95 26 649.0 0 26 96 17 649.0 0 17 97 27 649.0 0 27 98 24 649.0 0 24 99 26 649.0 0 26 100 20 649.0 0 20 101 19 649.0 0 19 102 30 649.0 0 30 103 51 649.0 0 51 104 108 649.0 0 108 105 35 649.0 0 35 106 29 649.0 0 29 107 74 649.0 0 74 108 45 649.0 0 45 109 49 649.0 0 49 110 65 649.0 0 65 111 156 649.0 0 156 112 131 649.0 0 131 113 284 649.0 0 284 114 74 649.0 0 74 115 215 649.0 0 215 116 411 649.0 0 411 117 5206 649.0 0 5206 118 3697 649.0 0 3697 119 4168 649.0 0 4168 120 43 649.0 0 43 121 30 649.0 0 30 122 16 649.0 0 16 123 12 649.0 0 12 124 19 649.0 0 19 125 28 649.0 0 28 126 42 649.0 0 42 127 20 649.0 0 20 128 26 649.0 0 26 129 24 649.0 0 24 130 30 649.0 0 30 131 39 649.0 0 39 132 29 649.0 0 29 133 41 649.0 0 41 134 24 649.0 0 24 135 14 649.0 0 14 136 12 649.0 0 12 137 26 649.0 0 26 138 27 649.0 0 27 139 21 649.0 0 21 140 24 649.0 0 24 141 16 649.0 0 16 142 16 649.0 0 16 143 16 649.0 0 16 144 21 649.0 0 21 145 27 649.0 0 27 146 46 649.0 0 46 147 31 649.0 0 31 148 23 649.0 0 23 149 27 649.0 0 27 150 21 649.0 0 21 151 324 649.0 0 324 152 194 649.0 0 194 153 238 649.0 0 238 154 100 649.0 0 100 155 60 649.0 0 60 156 22 649.0 0 22 157 16 649.0 0 16 158 13 649.0 0 13 159 14 649.0 0 14 160 23 649.0 0 23 161 27 649.0 0 27 162 43 649.0 0 43 163 36 649.0 0 36 164 33 649.0 0 33 165 17 649.0 0 17 166 23 649.0 0 23 167 14 649.0 0 14 168 7 649.0 0 7 169 19 649.0 0 19 170 42 649.0 0 42 171 48 649.0 0 48 172 24 649.0 0 24 173 19 649.0 0 19 174 15 649.0 0 15 175 11 649.0 0 11 176 23 649.0 0 23 177 17 649.0 0 17 178 24 649.0 0 24 179 56 649.0 0 56 180 104 649.0 0 104 181 57 649.0 0 57 182 104 649.0 0 104 183 146 649.0 0 146 184 87 649.0 0 87 185 273 649.0 0 273 186 19 649.0 0 19 187 17 649.0 0 17 188 11 649.0 0 11 189 17 649.0 0 17 190 49 649.0 0 49 191 43 649.0 0 43 192 20 649.0 0 20 193 17 649.0 0 17 194 11 649.0 0 11 195 15 649.0 0 15 196 20 649.0 0 20 197 17 649.0 0 17 198 24 649.0 0 24 199 18 649.0 0 18 200 19 649.0 0 19 201 23 649.0 0 23 202 14 649.0 0 14 203 21 649.0 0 21 204 25 649.0 0 25 205 15 649.0 0 15 206 18 649.0 0 18 207 19 649.0 0 19 208 14 649.0 0 14 209 11 649.0 0 11 210 16 649.0 0 16 211 22 649.0 0 22 212 31 649.0 0 31 213 21 649.0 0 21 214 43 649.0 0 43 215 24 649.0 0 24 216 39 649.0 0 39 217 113 649.0 0 113 218 230 649.0 0 230 219 251 649.0 0 251 220 242 649.0 0 242 221 54 649.0 0 54 222 21 649.0 0 21 223 52 649.0 0 52 224 27 649.0 0 27 225 52 649.0 0 52 226 85 649.0 0 85 227 39 649.0 0 39 228 85 649.0 0 85 229 59 649.0 0 59 230 25 649.0 0 25 231 84 649.0 0 84 232 37 649.0 0 37 233 73 649.0 0 73 234 73 649.0 0 73 235 55 649.0 0 55 236 50 649.0 0 50 237 39 649.0 0 39 238 46 649.0 0 46 239 37 649.0 0 37 240 19 649.0 0 19 241 20 649.0 0 20 242 18 649.0 0 18 243 23 649.0 0 23 244 50 649.0 0 50 245 54 649.0 0 54 246 62 649.0 0 62 247 75 649.0 0 75 248 36 649.0 0 36 249 25 649.0 0 25 250 38 649.0 0 38 251 39 649.0 0 39 252 45 649.0 0 45 253 49 649.0 0 49 254 46 649.0 0 46 255 62 649.0 0 62 256 50 649.0 0 50 257 28 649.0 0 28 258 9 649.0 0 9 259 34 649.0 0 34 260 27 649.0 0 27 261 25 649.0 0 25 262 15 649.0 0 15 263 22 649.0 0 22 264 21 649.0 0 21 265 19 649.0 0 19 266 39 649.0 0 39 267 23 649.0 0 23 268 28 649.0 0 28 269 20 649.0 0 20 270 18 649.0 0 18 271 28 649.0 0 28 272 42 649.0 0 42 273 35 649.0 0 35 274 31 649.0 0 31 275 23 649.0 0 23 276 25 649.0 0 25 277 38 649.0 0 38 278 66 649.0 0 66 279 79 649.0 0 79 280 38 649.0 0 38 281 29 649.0 0 29 282 29 649.0 0 29 283 32 649.0 0 32 284 50 649.0 0 50 285 48 649.0 0 48 286 64 649.0 0 64 287 52 649.0 0 52 288 40 649.0 0 40 289 30 649.0 0 30 290 25 649.0 0 25 291 40 649.0 0 40 292 49 649.0 0 49 293 81 649.0 0 81 294 40 649.0 0 40 295 28 649.0 0 28 296 27 649.0 0 27 297 21 649.0 0 21 298 46 649.0 0 46 299 27 649.0 0 27 300 65 649.0 0 65 301 47 649.0 0 47 Finished in 3.25 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 63,214 Reads with adapters: 23,636 (37.4%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 23,611 (37.4%) Total basepairs processed: 16,256,913 bp Total written (filtered): 6,385,582 bp (39.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23636 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 261 0.0 3 0 253 5 2 1 20 22896 0.0 4 22431 444 18 3 21 64 0.0 4 19 45 22 4 0.0 4 1 0 1 1 1 23 15 0.0 4 0 0 0 14 1 29 1 0.0 4 0 1 41 1 0.0 4 1 53 2 0.0 4 0 0 0 0 2 62 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 350 0.0 4 0 0 0 0 350 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 154 3 0.0 4 0 0 0 0 3 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 1 228 8 0.0 4 0 0 0 0 8 229 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 23,611 Reads with adapters: 3,470 (14.7%) Reads written (passing filters): 23,611 (100.0%) Total basepairs processed: 6,385,582 bp Total written (filtered): 6,351,494 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3470 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.1% G: 94.0% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 101 368.9 0 101 4 6 92.2 0 6 5 1 23.1 0 1 6 8 5.8 0 8 9 1678 0.1 0 52 1626 10 1333 0.0 1 53 1280 11 330 0.0 1 5 325 12 3 0.0 1 0 3 13 1 0.0 1 0 1 93 1 0.0 1 1 150 2 0.0 1 0 2 151 1 0.0 1 0 1 152 1 0.0 1 0 1 208 1 0.0 1 0 1 223 1 0.0 1 1 235 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.