This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.20 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 434,838 (16.4%) Reads that were too short: 46,269 (1.7%) Reads written (passing filters): 388,569 (14.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 100,425,837 bp (12.6%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 434838 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1265 649.0 0 1265 7 40373 649.0 0 40373 8 32142 649.0 0 32142 9 260 649.0 0 260 10 269 649.0 0 269 11 156 649.0 0 156 12 147 649.0 0 147 13 334 649.0 0 334 14 448 649.0 0 448 15 442 649.0 0 442 16 389 649.0 0 389 17 679 649.0 0 679 18 889 649.0 0 889 19 1502 649.0 0 1502 20 29417 649.0 0 29417 21 18435 649.0 0 18435 22 32470 649.0 0 32470 23 254 649.0 0 254 24 161 649.0 0 161 25 167 649.0 0 167 26 86 649.0 0 86 27 119 649.0 0 119 28 91 649.0 0 91 29 104 649.0 0 104 30 78 649.0 0 78 31 114 649.0 0 114 32 174 649.0 0 174 33 277 649.0 0 277 34 297 649.0 0 297 35 644 649.0 0 644 36 1293 649.0 0 1293 37 318 649.0 0 318 38 403 649.0 0 403 39 799 649.0 0 799 40 378 649.0 0 378 41 374 649.0 0 374 42 668 649.0 0 668 43 725 649.0 0 725 44 986 649.0 0 986 45 853 649.0 0 853 46 1478 649.0 0 1478 47 1970 649.0 0 1970 48 4085 649.0 0 4085 49 71840 649.0 0 71840 50 48025 649.0 0 48025 51 63532 649.0 0 63532 52 277 649.0 0 277 53 60 649.0 0 60 54 46 649.0 0 46 55 43 649.0 0 43 56 69 649.0 0 69 57 68 649.0 0 68 58 80 649.0 0 80 59 36 649.0 0 36 60 90 649.0 0 90 61 76 649.0 0 76 62 138 649.0 0 138 63 1216 649.0 0 1216 64 866 649.0 0 866 65 1573 649.0 0 1573 66 46 649.0 0 46 67 49 649.0 0 49 68 53 649.0 0 53 69 35 649.0 0 35 70 44 649.0 0 44 71 43 649.0 0 43 72 37 649.0 0 37 73 37 649.0 0 37 74 53 649.0 0 53 75 39 649.0 0 39 76 49 649.0 0 49 77 47 649.0 0 47 78 29 649.0 0 29 79 39 649.0 0 39 80 39 649.0 0 39 81 37 649.0 0 37 82 27 649.0 0 27 83 41 649.0 0 41 84 49 649.0 0 49 85 35 649.0 0 35 86 44 649.0 0 44 87 36 649.0 0 36 88 57 649.0 0 57 89 55 649.0 0 55 90 183 649.0 0 183 91 54 649.0 0 54 92 56 649.0 0 56 93 162 649.0 0 162 94 41 649.0 0 41 95 35 649.0 0 35 96 65 649.0 0 65 97 38 649.0 0 38 98 46 649.0 0 46 99 34 649.0 0 34 100 25 649.0 0 25 101 35 649.0 0 35 102 40 649.0 0 40 103 19 649.0 0 19 104 23 649.0 0 23 105 25 649.0 0 25 106 53 649.0 0 53 107 46 649.0 0 46 108 32 649.0 0 32 109 30 649.0 0 30 110 39 649.0 0 39 111 34 649.0 0 34 112 45 649.0 0 45 113 33 649.0 0 33 114 32 649.0 0 32 115 63 649.0 0 63 116 70 649.0 0 70 117 207 649.0 0 207 118 268 649.0 0 268 119 385 649.0 0 385 120 95 649.0 0 95 121 58 649.0 0 58 122 46 649.0 0 46 123 68 649.0 0 68 124 490 649.0 0 490 125 143 649.0 0 143 126 231 649.0 0 231 127 74 649.0 0 74 128 44 649.0 0 44 129 40 649.0 0 40 130 46 649.0 0 46 131 39 649.0 0 39 132 50 649.0 0 50 133 34 649.0 0 34 134 28 649.0 0 28 135 49 649.0 0 49 136 124 649.0 0 124 137 43 649.0 0 43 138 51 649.0 0 51 139 45 649.0 0 45 140 55 649.0 0 55 141 66 649.0 0 66 142 66 649.0 0 66 143 59 649.0 0 59 144 84 649.0 0 84 145 67 649.0 0 67 146 65 649.0 0 65 147 51 649.0 0 51 148 46 649.0 0 46 149 40 649.0 0 40 150 55 649.0 0 55 151 41 649.0 0 41 152 85 649.0 0 85 153 89 649.0 0 89 154 82 649.0 0 82 155 58 649.0 0 58 156 45 649.0 0 45 157 93 649.0 0 93 158 100 649.0 0 100 159 109 649.0 0 109 160 94 649.0 0 94 161 62 649.0 0 62 162 44 649.0 0 44 163 46 649.0 0 46 164 43 649.0 0 43 165 48 649.0 0 48 166 41 649.0 0 41 167 50 649.0 0 50 168 51 649.0 0 51 169 47 649.0 0 47 170 63 649.0 0 63 171 67 649.0 0 67 172 75 649.0 0 75 173 73 649.0 0 73 174 55 649.0 0 55 175 53 649.0 0 53 176 80 649.0 0 80 177 124 649.0 0 124 178 144 649.0 0 144 179 146 649.0 0 146 180 237 649.0 0 237 181 338 649.0 0 338 182 4788 649.0 0 4788 183 420 649.0 0 420 184 2199 649.0 0 2199 185 74 649.0 0 74 186 71 649.0 0 71 187 72 649.0 0 72 188 146 649.0 0 146 189 145 649.0 0 145 190 362 649.0 0 362 191 157 649.0 0 157 192 160 649.0 0 160 193 1301 649.0 0 1301 194 2245 649.0 0 2245 195 2097 649.0 0 2097 196 786 649.0 0 786 197 240 649.0 0 240 198 128 649.0 0 128 199 65 649.0 0 65 200 83 649.0 0 83 201 109 649.0 0 109 202 73 649.0 0 73 203 43 649.0 0 43 204 52 649.0 0 52 205 221 649.0 0 221 206 115 649.0 0 115 207 80 649.0 0 80 208 93 649.0 0 93 209 86 649.0 0 86 210 74 649.0 0 74 211 97 649.0 0 97 212 88 649.0 0 88 213 515 649.0 0 515 214 1670 649.0 0 1670 215 1037 649.0 0 1037 216 1312 649.0 0 1312 217 211 649.0 0 211 218 203 649.0 0 203 219 559 649.0 0 559 220 521 649.0 0 521 221 476 649.0 0 476 222 149 649.0 0 149 223 151 649.0 0 151 224 68 649.0 0 68 225 114 649.0 0 114 226 113 649.0 0 113 227 136 649.0 0 136 228 90 649.0 0 90 229 91 649.0 0 91 230 96 649.0 0 96 231 160 649.0 0 160 232 104 649.0 0 104 233 190 649.0 0 190 234 202 649.0 0 202 235 97 649.0 0 97 236 195 649.0 0 195 237 159 649.0 0 159 238 251 649.0 0 251 239 1097 649.0 0 1097 240 1360 649.0 0 1360 241 1072 649.0 0 1072 242 619 649.0 0 619 243 55 649.0 0 55 244 93 649.0 0 93 245 101 649.0 0 101 246 216 649.0 0 216 247 117 649.0 0 117 248 141 649.0 0 141 249 208 649.0 0 208 250 730 649.0 0 730 251 912 649.0 0 912 252 1197 649.0 0 1197 253 1294 649.0 0 1294 254 149 649.0 0 149 255 91 649.0 0 91 256 171 649.0 0 171 257 341 649.0 0 341 258 215 649.0 0 215 259 421 649.0 0 421 260 253 649.0 0 253 261 284 649.0 0 284 262 288 649.0 0 288 263 2109 649.0 0 2109 264 956 649.0 0 956 265 2531 649.0 0 2531 266 358 649.0 0 358 267 334 649.0 0 334 268 295 649.0 0 295 269 218 649.0 0 218 270 298 649.0 0 298 271 135 649.0 0 135 272 143 649.0 0 143 273 86 649.0 0 86 274 62 649.0 0 62 275 174 649.0 0 174 276 129 649.0 0 129 277 127 649.0 0 127 278 123 649.0 0 123 279 82 649.0 0 82 280 108 649.0 0 108 281 159 649.0 0 159 282 189 649.0 0 189 283 437 649.0 0 437 284 307 649.0 0 307 285 423 649.0 0 423 286 278 649.0 0 278 287 346 649.0 0 346 288 4565 649.0 0 4565 289 2964 649.0 0 2964 290 3566 649.0 0 3566 291 1035 649.0 0 1035 292 182 649.0 0 182 293 284 649.0 0 284 294 317 649.0 0 317 295 241 649.0 0 241 296 94 649.0 0 94 297 258 649.0 0 258 298 202 649.0 0 202 299 271 649.0 0 271 300 321 649.0 0 321 301 245 649.0 0 245 Finished in 16.45 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 388,569 Reads with adapters: 48,199 (12.4%) Reads that were too short: 1,795 (0.5%) Reads written (passing filters): 46,404 (11.9%) Total basepairs processed: 100,425,837 bp Total written (filtered): 11,756,921 bp (11.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 48199 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 5 5 16 301 0.0 3 0 250 36 15 17 38405 0.0 3 36304 1731 295 75 18 124 0.0 3 10 109 4 1 19 89 0.0 3 1 0 59 29 20 106 0.0 3 2 0 2 102 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 6 0.0 3 0 0 0 6 24 2 0.0 3 0 0 0 2 27 2 0.0 3 0 0 0 2 28 5 0.0 3 1 0 0 4 29 5 0.0 3 0 0 1 4 31 7 0.0 3 0 0 0 7 32 3 0.0 3 0 0 0 3 34 14 0.0 3 0 1 1 12 35 5 0.0 3 0 0 0 5 37 2 0.0 3 0 0 0 2 40 2 0.0 3 0 0 0 2 41 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 14 0.0 3 0 0 0 14 47 7 0.0 3 0 0 1 6 48 1 0.0 3 0 0 0 1 49 11 0.0 3 0 0 0 11 50 6 0.0 3 1 0 1 4 51 20 0.0 3 0 0 0 20 52 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 57 33 0.0 3 0 0 0 33 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 5 0.0 3 0 0 1 4 61 3 0.0 3 0 0 1 2 63 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 1 3 65 5 0.0 3 0 0 0 5 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 5 0.0 3 0 0 0 5 73 587 0.0 3 0 0 2 585 74 30 0.0 3 0 0 0 30 75 4 0.0 3 0 0 0 4 76 5 0.0 3 0 0 0 5 79 2 0.0 3 0 0 0 2 80 3 0.0 3 0 0 0 3 81 4 0.0 3 0 0 0 4 83 2 0.0 3 0 0 0 2 87 15 0.0 3 0 0 0 15 88 3 0.0 3 0 0 0 3 89 2 0.0 3 0 0 0 2 90 13 0.0 3 0 0 0 13 91 11 0.0 3 0 0 2 9 92 10 0.0 3 0 0 0 10 93 66 0.0 3 0 1 57 8 94 11 0.0 3 0 0 1 10 95 2 0.0 3 0 0 1 1 97 36 0.0 3 1 0 0 35 98 6 0.0 3 0 0 0 6 99 5 0.0 3 0 0 0 5 100 10 0.0 3 0 0 0 10 101 18 0.0 3 0 0 0 18 102 45 0.0 3 0 1 0 44 103 16 0.0 3 0 0 0 16 104 8 0.0 3 0 0 0 8 105 4 0.0 3 0 0 0 4 106 4 0.0 3 0 0 0 4 107 5 0.0 3 0 0 0 5 108 5 0.0 3 0 0 0 5 109 3 0.0 3 0 0 2 1 110 1 0.0 3 0 0 0 1 111 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 116 5 0.0 3 0 0 0 5 117 62 0.0 3 0 0 0 62 118 8 0.0 3 0 0 0 8 119 6 0.0 3 0 0 1 5 120 12 0.0 3 0 0 0 12 121 15 0.0 3 0 0 0 15 122 916 0.0 3 0 0 8 908 123 919 0.0 3 0 0 6 913 124 633 0.0 3 0 0 13 620 125 224 0.0 3 0 0 7 217 126 182 0.0 3 0 0 5 177 127 65 0.0 3 0 0 0 65 128 196 0.0 3 0 0 1 195 129 37 0.0 3 0 0 1 36 130 26 0.0 3 0 0 0 26 131 34 0.0 3 0 0 2 32 132 6 0.0 3 0 1 0 5 133 9 0.0 3 0 0 0 9 134 16 0.0 3 0 0 0 16 135 13 0.0 3 0 0 0 13 136 14 0.0 3 0 0 0 14 137 71 0.0 3 0 0 1 70 138 23 0.0 3 0 0 0 23 139 53 0.0 3 0 0 1 52 140 27 0.0 3 0 0 0 27 141 13 0.0 3 0 0 0 13 142 384 0.0 3 0 0 4 380 143 51 0.0 3 0 0 2 49 144 6 0.0 3 0 0 0 6 145 54 0.0 3 0 0 2 52 146 89 0.0 3 0 1 0 88 147 10 0.0 3 0 0 0 10 148 32 0.0 3 0 0 0 32 149 52 0.0 3 0 0 2 50 150 15 0.0 3 0 0 0 15 151 196 0.0 3 0 0 4 192 152 94 0.0 3 0 0 2 92 153 64 0.0 3 0 0 1 63 154 35 0.0 3 0 0 1 34 155 19 0.0 3 0 0 0 19 156 8 0.0 3 0 0 0 8 157 9 0.0 3 0 0 0 9 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 3 0.0 3 1 0 0 2 161 10 0.0 3 0 0 0 10 162 3 0.0 3 0 0 0 3 163 14 0.0 3 0 0 0 14 164 8 0.0 3 0 0 0 8 165 13 0.0 3 0 0 0 13 166 34 0.0 3 0 0 3 31 167 8 0.0 3 0 0 0 8 168 27 0.0 3 0 0 0 27 169 7 0.0 3 0 0 0 7 170 841 0.0 3 0 0 14 827 171 388 0.0 3 0 0 6 382 172 211 0.0 3 0 0 1 210 173 20 0.0 3 0 0 0 20 174 82 0.0 3 0 0 2 80 175 47 0.0 3 0 0 0 47 176 5 0.0 3 0 0 1 4 177 32 0.0 3 0 0 0 32 178 3 0.0 3 0 0 0 3 179 12 0.0 3 0 0 1 11 180 83 0.0 3 0 0 1 82 181 4 0.0 3 0 0 0 4 182 3 0.0 3 0 0 0 3 183 14 0.0 3 0 0 0 14 184 5 0.0 3 0 0 0 5 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 6 0.0 3 0 0 0 6 189 7 0.0 3 0 0 0 7 190 6 0.0 3 0 0 0 6 191 5 0.0 3 0 0 0 5 192 3 0.0 3 0 0 1 2 193 19 0.0 3 0 0 1 18 194 7 0.0 3 0 0 0 7 195 3 0.0 3 0 0 0 3 196 4 0.0 3 0 0 0 4 197 12 0.0 3 0 0 1 11 198 4 0.0 3 0 0 0 4 199 3 0.0 3 0 0 0 3 200 1 0.0 3 0 0 0 1 201 3 0.0 3 0 0 0 3 202 21 0.0 3 0 0 1 20 203 7 0.0 3 0 0 0 7 204 4 0.0 3 0 0 1 3 205 2 0.0 3 0 0 1 1 206 7 0.0 3 0 0 0 7 207 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 210 6 0.0 3 0 0 0 6 211 2 0.0 3 0 0 1 1 212 6 0.0 3 0 0 0 6 213 1 0.0 3 0 0 0 1 214 7 0.0 3 0 0 0 7 215 6 0.0 3 0 0 0 6 216 2 0.0 3 0 0 1 1 217 7 0.0 3 0 0 0 7 218 10 0.0 3 0 0 0 10 219 2 0.0 3 0 0 0 2 220 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 222 12 0.0 3 0 0 1 11 223 10 0.0 3 0 0 0 10 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 226 6 0.0 3 0 0 0 6 227 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 229 3 0.0 3 0 0 0 3 230 4 0.0 3 0 0 1 3 231 16 0.0 3 0 0 0 16 232 11 0.0 3 0 0 0 11 233 15 0.0 3 0 0 3 12 234 88 0.0 3 0 0 3 85 235 178 0.0 3 0 0 1 177 236 96 0.0 3 0 0 5 91 237 76 0.0 3 0 0 5 71 238 164 0.0 3 0 0 7 157 239 60 0.0 3 0 0 0 60 240 15 0.0 3 0 0 0 15 241 20 0.0 3 0 0 0 20 242 27 0.0 3 0 0 0 27 243 51 0.0 3 0 0 3 48 244 91 0.0 3 0 0 6 85 245 15 0.0 3 0 0 0 15 246 8 0.0 3 0 0 0 8 247 5 0.0 3 0 0 0 5 248 9 0.0 3 0 0 0 9 249 11 0.0 3 0 0 0 11 250 19 0.0 3 0 0 1 18 251 6 0.0 3 0 0 1 5 252 24 0.0 3 0 0 0 24 253 2 0.0 3 0 0 0 2 254 6 0.0 3 0 0 0 6 257 1 0.0 3 0 0 0 1 258 5 0.0 3 0 0 0 5 259 2 0.0 3 0 0 0 2 260 5 0.0 3 0 0 0 5 261 5 0.0 3 0 0 0 5 262 6 0.0 3 0 0 0 6 263 20 0.0 3 0 0 0 20 264 14 0.0 3 0 0 0 14 265 4 0.0 3 0 0 0 4 266 14 0.0 3 0 0 2 12 267 19 0.0 3 0 0 1 18 268 5 0.0 3 0 0 1 4 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 9 0.0 3 0 0 0 9 272 28 0.0 3 0 0 2 26 273 18 0.0 3 0 0 0 18 274 5 0.0 3 0 0 0 5 278 2 0.0 3 0 0 0 2 279 6 0.0 3 0 0 0 6 280 5 0.0 3 0 0 0 5 281 12 0.0 3 0 0 0 12 282 6 0.0 3 0 0 0 6 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.56 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 46,404 Reads with adapters: 873 (1.9%) Reads written (passing filters): 46,404 (100.0%) Total basepairs processed: 11,756,921 bp Total written (filtered): 11,754,255 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 873 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.2% C: 33.1% G: 27.8% T: 6.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 838 725.1 0 838 4 27 181.3 0 27 5 4 45.3 0 4 6 4 11.3 0 4