This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.83 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 137,691 (5.2%) Reads that were too short: 40,005 (1.5%) Reads written (passing filters): 97,686 (3.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 21,875,974 bp (2.7%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 137691 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 748 649.0 0 748 7 916 649.0 0 916 8 18933 649.0 0 18933 9 15939 649.0 0 15939 10 115 649.0 0 115 11 77 649.0 0 77 12 70 649.0 0 70 13 55 649.0 0 55 14 70 649.0 0 70 15 94 649.0 0 94 16 94 649.0 0 94 17 97 649.0 0 97 18 86 649.0 0 86 19 116 649.0 0 116 20 87 649.0 0 87 21 73 649.0 0 73 22 61 649.0 0 61 23 72 649.0 0 72 24 105 649.0 0 105 25 84 649.0 0 84 26 97 649.0 0 97 27 97 649.0 0 97 28 106 649.0 0 106 29 73 649.0 0 73 30 91 649.0 0 91 31 110 649.0 0 110 32 147 649.0 0 147 33 131 649.0 0 131 34 101 649.0 0 101 35 161 649.0 0 161 36 143 649.0 0 143 37 1337 649.0 0 1337 38 1136 649.0 0 1136 39 1815 649.0 0 1815 40 277 649.0 0 277 41 115 649.0 0 115 42 106 649.0 0 106 43 135 649.0 0 135 44 128 649.0 0 128 45 114 649.0 0 114 46 129 649.0 0 129 47 91 649.0 0 91 48 90 649.0 0 90 49 96 649.0 0 96 50 70 649.0 0 70 51 84 649.0 0 84 52 95 649.0 0 95 53 74 649.0 0 74 54 88 649.0 0 88 55 122 649.0 0 122 56 101 649.0 0 101 57 100 649.0 0 100 58 91 649.0 0 91 59 79 649.0 0 79 60 95 649.0 0 95 61 90 649.0 0 90 62 83 649.0 0 83 63 114 649.0 0 114 64 117 649.0 0 117 65 88 649.0 0 88 66 108 649.0 0 108 67 103 649.0 0 103 68 83 649.0 0 83 69 122 649.0 0 122 70 112 649.0 0 112 71 127 649.0 0 127 72 128 649.0 0 128 73 143 649.0 0 143 74 125 649.0 0 125 75 163 649.0 0 163 76 288 649.0 0 288 77 238 649.0 0 238 78 1096 649.0 0 1096 79 307 649.0 0 307 80 129 649.0 0 129 81 92 649.0 0 92 82 140 649.0 0 140 83 100 649.0 0 100 84 113 649.0 0 113 85 90 649.0 0 90 86 114 649.0 0 114 87 82 649.0 0 82 88 219 649.0 0 219 89 229 649.0 0 229 90 219 649.0 0 219 91 362 649.0 0 362 92 887 649.0 0 887 93 722 649.0 0 722 94 479 649.0 0 479 95 343 649.0 0 343 96 87 649.0 0 87 97 117 649.0 0 117 98 88 649.0 0 88 99 103 649.0 0 103 100 99 649.0 0 99 101 83 649.0 0 83 102 80 649.0 0 80 103 168 649.0 0 168 104 310 649.0 0 310 105 107 649.0 0 107 106 278 649.0 0 278 107 123 649.0 0 123 108 105 649.0 0 105 109 134 649.0 0 134 110 163 649.0 0 163 111 112 649.0 0 112 112 232 649.0 0 232 113 187 649.0 0 187 114 246 649.0 0 246 115 205 649.0 0 205 116 207 649.0 0 207 117 137 649.0 0 137 118 129 649.0 0 129 119 107 649.0 0 107 120 112 649.0 0 112 121 117 649.0 0 117 122 129 649.0 0 129 123 124 649.0 0 124 124 157 649.0 0 157 125 109 649.0 0 109 126 143 649.0 0 143 127 191 649.0 0 191 128 310 649.0 0 310 129 2799 649.0 0 2799 130 1124 649.0 0 1124 131 852 649.0 0 852 132 215 649.0 0 215 133 160 649.0 0 160 134 161 649.0 0 161 135 215 649.0 0 215 136 237 649.0 0 237 137 258 649.0 0 258 138 226 649.0 0 226 139 452 649.0 0 452 140 359 649.0 0 359 141 945 649.0 0 945 142 11797 649.0 0 11797 143 4536 649.0 0 4536 144 8847 649.0 0 8847 145 148 649.0 0 148 146 103 649.0 0 103 147 90 649.0 0 90 148 102 649.0 0 102 149 210 649.0 0 210 150 176 649.0 0 176 151 196 649.0 0 196 152 85 649.0 0 85 153 121 649.0 0 121 154 123 649.0 0 123 155 100 649.0 0 100 156 112 649.0 0 112 157 101 649.0 0 101 158 95 649.0 0 95 159 86 649.0 0 86 160 79 649.0 0 79 161 80 649.0 0 80 162 79 649.0 0 79 163 94 649.0 0 94 164 91 649.0 0 91 165 88 649.0 0 88 166 81 649.0 0 81 167 90 649.0 0 90 168 93 649.0 0 93 169 109 649.0 0 109 170 80 649.0 0 80 171 74 649.0 0 74 172 60 649.0 0 60 173 86 649.0 0 86 174 90 649.0 0 90 175 93 649.0 0 93 176 83 649.0 0 83 177 123 649.0 0 123 178 201 649.0 0 201 179 236 649.0 0 236 180 76 649.0 0 76 181 75 649.0 0 75 182 83 649.0 0 83 183 105 649.0 0 105 184 71 649.0 0 71 185 88 649.0 0 88 186 112 649.0 0 112 187 91 649.0 0 91 188 95 649.0 0 95 189 91 649.0 0 91 190 135 649.0 0 135 191 100 649.0 0 100 192 106 649.0 0 106 193 80 649.0 0 80 194 69 649.0 0 69 195 70 649.0 0 70 196 86 649.0 0 86 197 95 649.0 0 95 198 84 649.0 0 84 199 85 649.0 0 85 200 71 649.0 0 71 201 106 649.0 0 106 202 150 649.0 0 150 203 164 649.0 0 164 204 122 649.0 0 122 205 111 649.0 0 111 206 97 649.0 0 97 207 242 649.0 0 242 208 194 649.0 0 194 209 1609 649.0 0 1609 210 447 649.0 0 447 211 899 649.0 0 899 212 160 649.0 0 160 213 235 649.0 0 235 214 1197 649.0 0 1197 215 903 649.0 0 903 216 1712 649.0 0 1712 217 196 649.0 0 196 218 402 649.0 0 402 219 120 649.0 0 120 220 203 649.0 0 203 221 156 649.0 0 156 222 186 649.0 0 186 223 148 649.0 0 148 224 159 649.0 0 159 225 143 649.0 0 143 226 257 649.0 0 257 227 253 649.0 0 253 228 389 649.0 0 389 229 1685 649.0 0 1685 230 654 649.0 0 654 231 1226 649.0 0 1226 232 375 649.0 0 375 233 495 649.0 0 495 234 4385 649.0 0 4385 235 3875 649.0 0 3875 236 5069 649.0 0 5069 237 2324 649.0 0 2324 238 107 649.0 0 107 239 69 649.0 0 69 240 136 649.0 0 136 241 100 649.0 0 100 242 69 649.0 0 69 243 88 649.0 0 88 244 73 649.0 0 73 245 61 649.0 0 61 246 51 649.0 0 51 247 94 649.0 0 94 248 76 649.0 0 76 249 49 649.0 0 49 250 80 649.0 0 80 251 65 649.0 0 65 252 60 649.0 0 60 253 134 649.0 0 134 254 189 649.0 0 189 255 159 649.0 0 159 256 119 649.0 0 119 257 66 649.0 0 66 258 70 649.0 0 70 259 73 649.0 0 73 260 91 649.0 0 91 261 109 649.0 0 109 262 305 649.0 0 305 263 159 649.0 0 159 264 237 649.0 0 237 265 140 649.0 0 140 266 127 649.0 0 127 267 60 649.0 0 60 268 59 649.0 0 59 269 83 649.0 0 83 270 140 649.0 0 140 271 141 649.0 0 141 272 169 649.0 0 169 273 80 649.0 0 80 274 75 649.0 0 75 275 88 649.0 0 88 276 108 649.0 0 108 277 81 649.0 0 81 278 105 649.0 0 105 279 82 649.0 0 82 280 73 649.0 0 73 281 101 649.0 0 101 282 86 649.0 0 86 283 87 649.0 0 87 284 125 649.0 0 125 285 81 649.0 0 81 286 82 649.0 0 82 287 79 649.0 0 79 288 64 649.0 0 64 289 62 649.0 0 62 290 58 649.0 0 58 291 146 649.0 0 146 292 87 649.0 0 87 293 127 649.0 0 127 294 105 649.0 0 105 295 346 649.0 0 346 296 774 649.0 0 774 297 854 649.0 0 854 298 709 649.0 0 709 299 389 649.0 0 389 300 89 649.0 0 89 301 112 649.0 0 112 Finished in 4.13 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 97,686 Reads with adapters: 19,607 (20.1%) Reads that were too short: 87 (0.1%) Reads written (passing filters): 19,520 (20.0%) Total basepairs processed: 21,875,974 bp Total written (filtered): 5,362,863 bp (24.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19607 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 5 5 16 170 0.0 3 0 147 11 12 17 18730 0.0 3 17729 833 135 33 18 61 0.0 3 9 34 8 10 19 45 0.0 3 1 3 21 20 20 319 0.0 3 238 11 4 66 21 2 0.0 3 1 1 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 25 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 1 41 1 0.0 3 0 0 0 1 42 11 0.0 3 0 0 1 10 43 43 0.0 3 0 0 0 43 44 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 19 0.0 3 0 0 0 19 51 4 0.0 3 0 0 0 4 53 8 0.0 3 0 0 0 8 54 2 0.0 3 0 0 0 2 56 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 61 3 0.0 3 0 0 0 3 63 8 0.0 3 0 0 0 8 66 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 79 3 0.0 3 0 0 0 3 82 3 0.0 3 0 0 0 3 83 2 0.0 3 1 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 10 0.0 3 0 0 0 10 102 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 1 2 145 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 5 0.0 3 0 0 1 4 157 4 0.0 3 0 0 0 4 158 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 164 2 0.0 3 1 0 0 1 165 1 0.0 3 0 1 169 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 182 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 5 0.0 3 0 0 1 4 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 1 241 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 256 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 262 3 0.0 3 0 0 0 3 263 2 0.0 3 0 0 0 2 266 1 0.0 3 0 0 1 270 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 19,520 Reads with adapters: 287 (1.5%) Reads written (passing filters): 19,520 (100.0%) Total basepairs processed: 5,362,863 bp Total written (filtered): 5,361,982 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 287 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.3% C: 7.7% G: 63.8% T: 7.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 273 305.0 0 273 4 8 76.2 0 8 5 6 19.1 0 6