This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.25 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 113,505 (4.3%) Reads that were too short: 21,116 (0.8%) Reads written (passing filters): 92,389 (3.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 20,874,228 bp (2.6%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 113505 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 708 649.0 0 708 7 759 649.0 0 759 8 17825 649.0 0 17825 9 18390 649.0 0 18390 10 76 649.0 0 76 11 68 649.0 0 68 12 45 649.0 0 45 13 72 649.0 0 72 14 60 649.0 0 60 15 62 649.0 0 62 16 68 649.0 0 68 17 72 649.0 0 72 18 59 649.0 0 59 19 77 649.0 0 77 20 56 649.0 0 56 21 76 649.0 0 76 22 62 649.0 0 62 23 77 649.0 0 77 24 62 649.0 0 62 25 57 649.0 0 57 26 78 649.0 0 78 27 82 649.0 0 82 28 72 649.0 0 72 29 50 649.0 0 50 30 69 649.0 0 69 31 72 649.0 0 72 32 101 649.0 0 101 33 90 649.0 0 90 34 97 649.0 0 97 35 101 649.0 0 101 36 109 649.0 0 109 37 840 649.0 0 840 38 986 649.0 0 986 39 1464 649.0 0 1464 40 85 649.0 0 85 41 65 649.0 0 65 42 89 649.0 0 89 43 114 649.0 0 114 44 109 649.0 0 109 45 91 649.0 0 91 46 92 649.0 0 92 47 100 649.0 0 100 48 61 649.0 0 61 49 102 649.0 0 102 50 77 649.0 0 77 51 92 649.0 0 92 52 79 649.0 0 79 53 73 649.0 0 73 54 75 649.0 0 75 55 76 649.0 0 76 56 90 649.0 0 90 57 66 649.0 0 66 58 80 649.0 0 80 59 81 649.0 0 81 60 72 649.0 0 72 61 60 649.0 0 60 62 59 649.0 0 59 63 73 649.0 0 73 64 82 649.0 0 82 65 102 649.0 0 102 66 65 649.0 0 65 67 81 649.0 0 81 68 90 649.0 0 90 69 78 649.0 0 78 70 98 649.0 0 98 71 95 649.0 0 95 72 75 649.0 0 75 73 118 649.0 0 118 74 82 649.0 0 82 75 136 649.0 0 136 76 284 649.0 0 284 77 172 649.0 0 172 78 1183 649.0 0 1183 79 273 649.0 0 273 80 137 649.0 0 137 81 68 649.0 0 68 82 79 649.0 0 79 83 67 649.0 0 67 84 56 649.0 0 56 85 78 649.0 0 78 86 52 649.0 0 52 87 52 649.0 0 52 88 151 649.0 0 151 89 202 649.0 0 202 90 128 649.0 0 128 91 270 649.0 0 270 92 423 649.0 0 423 93 568 649.0 0 568 94 310 649.0 0 310 95 263 649.0 0 263 96 79 649.0 0 79 97 84 649.0 0 84 98 62 649.0 0 62 99 65 649.0 0 65 100 67 649.0 0 67 101 69 649.0 0 69 102 72 649.0 0 72 103 124 649.0 0 124 104 337 649.0 0 337 105 79 649.0 0 79 106 296 649.0 0 296 107 90 649.0 0 90 108 73 649.0 0 73 109 98 649.0 0 98 110 148 649.0 0 148 111 117 649.0 0 117 112 178 649.0 0 178 113 140 649.0 0 140 114 197 649.0 0 197 115 169 649.0 0 169 116 179 649.0 0 179 117 93 649.0 0 93 118 86 649.0 0 86 119 76 649.0 0 76 120 86 649.0 0 86 121 72 649.0 0 72 122 112 649.0 0 112 123 88 649.0 0 88 124 107 649.0 0 107 125 93 649.0 0 93 126 106 649.0 0 106 127 138 649.0 0 138 128 247 649.0 0 247 129 3051 649.0 0 3051 130 1144 649.0 0 1144 131 985 649.0 0 985 132 163 649.0 0 163 133 110 649.0 0 110 134 124 649.0 0 124 135 146 649.0 0 146 136 208 649.0 0 208 137 218 649.0 0 218 138 186 649.0 0 186 139 343 649.0 0 343 140 289 649.0 0 289 141 825 649.0 0 825 142 11538 649.0 0 11538 143 4435 649.0 0 4435 144 9609 649.0 0 9609 145 129 649.0 0 129 146 66 649.0 0 66 147 55 649.0 0 55 148 78 649.0 0 78 149 126 649.0 0 126 150 121 649.0 0 121 151 108 649.0 0 108 152 66 649.0 0 66 153 81 649.0 0 81 154 77 649.0 0 77 155 73 649.0 0 73 156 79 649.0 0 79 157 101 649.0 0 101 158 53 649.0 0 53 159 47 649.0 0 47 160 58 649.0 0 58 161 59 649.0 0 59 162 40 649.0 0 40 163 70 649.0 0 70 164 81 649.0 0 81 165 52 649.0 0 52 166 64 649.0 0 64 167 47 649.0 0 47 168 60 649.0 0 60 169 98 649.0 0 98 170 76 649.0 0 76 171 44 649.0 0 44 172 52 649.0 0 52 173 65 649.0 0 65 174 74 649.0 0 74 175 64 649.0 0 64 176 70 649.0 0 70 177 72 649.0 0 72 178 153 649.0 0 153 179 220 649.0 0 220 180 88 649.0 0 88 181 52 649.0 0 52 182 71 649.0 0 71 183 85 649.0 0 85 184 37 649.0 0 37 185 39 649.0 0 39 186 71 649.0 0 71 187 52 649.0 0 52 188 64 649.0 0 64 189 62 649.0 0 62 190 108 649.0 0 108 191 74 649.0 0 74 192 63 649.0 0 63 193 57 649.0 0 57 194 55 649.0 0 55 195 53 649.0 0 53 196 69 649.0 0 69 197 74 649.0 0 74 198 53 649.0 0 53 199 53 649.0 0 53 200 46 649.0 0 46 201 62 649.0 0 62 202 108 649.0 0 108 203 95 649.0 0 95 204 73 649.0 0 73 205 80 649.0 0 80 206 55 649.0 0 55 207 130 649.0 0 130 208 95 649.0 0 95 209 652 649.0 0 652 210 154 649.0 0 154 211 353 649.0 0 353 212 82 649.0 0 82 213 113 649.0 0 113 214 460 649.0 0 460 215 393 649.0 0 393 216 574 649.0 0 574 217 103 649.0 0 103 218 197 649.0 0 197 219 72 649.0 0 72 220 105 649.0 0 105 221 89 649.0 0 89 222 122 649.0 0 122 223 83 649.0 0 83 224 122 649.0 0 122 225 98 649.0 0 98 226 130 649.0 0 130 227 130 649.0 0 130 228 179 649.0 0 179 229 996 649.0 0 996 230 332 649.0 0 332 231 709 649.0 0 709 232 169 649.0 0 169 233 300 649.0 0 300 234 1839 649.0 0 1839 235 1989 649.0 0 1989 236 2443 649.0 0 2443 237 1442 649.0 0 1442 238 81 649.0 0 81 239 60 649.0 0 60 240 137 649.0 0 137 241 45 649.0 0 45 242 31 649.0 0 31 243 52 649.0 0 52 244 63 649.0 0 63 245 66 649.0 0 66 246 61 649.0 0 61 247 60 649.0 0 60 248 56 649.0 0 56 249 54 649.0 0 54 250 43 649.0 0 43 251 52 649.0 0 52 252 47 649.0 0 47 253 80 649.0 0 80 254 103 649.0 0 103 255 59 649.0 0 59 256 71 649.0 0 71 257 63 649.0 0 63 258 54 649.0 0 54 259 65 649.0 0 65 260 67 649.0 0 67 261 79 649.0 0 79 262 341 649.0 0 341 263 123 649.0 0 123 264 204 649.0 0 204 265 79 649.0 0 79 266 88 649.0 0 88 267 43 649.0 0 43 268 52 649.0 0 52 269 43 649.0 0 43 270 104 649.0 0 104 271 76 649.0 0 76 272 77 649.0 0 77 273 56 649.0 0 56 274 64 649.0 0 64 275 59 649.0 0 59 276 70 649.0 0 70 277 81 649.0 0 81 278 70 649.0 0 70 279 56 649.0 0 56 280 43 649.0 0 43 281 67 649.0 0 67 282 53 649.0 0 53 283 65 649.0 0 65 284 78 649.0 0 78 285 70 649.0 0 70 286 64 649.0 0 64 287 93 649.0 0 93 288 63 649.0 0 63 289 61 649.0 0 61 290 47 649.0 0 47 291 125 649.0 0 125 292 72 649.0 0 72 293 84 649.0 0 84 294 79 649.0 0 79 295 171 649.0 0 171 296 465 649.0 0 465 297 411 649.0 0 411 298 367 649.0 0 367 299 149 649.0 0 149 300 54 649.0 0 54 301 134 649.0 0 134 Finished in 4.38 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 92,389 Reads with adapters: 19,937 (21.6%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 19,920 (21.6%) Total basepairs processed: 20,874,228 bp Total written (filtered): 5,352,584 bp (25.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19937 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 0 3 18 9 0.0 3 0 0 9 19 160 0.0 3 0 153 5 1 1 20 18999 0.0 4 18605 374 17 3 21 62 0.0 4 17 44 0 0 1 22 5 0.0 4 2 1 1 0 1 23 53 0.0 4 36 0 1 15 1 25 1 0.0 4 0 0 0 1 26 1 0.0 4 0 1 27 3 0.0 4 1 0 0 0 2 40 5 0.0 4 0 0 0 0 5 42 55 0.0 4 0 0 0 0 55 45 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 69 27 0.0 4 0 0 0 0 27 73 1 0.0 4 0 0 0 0 1 97 36 0.0 4 0 0 0 0 36 101 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 493 0.0 4 0 0 0 0 493 130 3 0.0 4 0 0 0 0 3 136 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 1 169 1 0.0 4 0 1 188 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.71 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 19,920 Reads with adapters: 2,559 (12.8%) Reads written (passing filters): 19,920 (100.0%) Total basepairs processed: 5,352,584 bp Total written (filtered): 5,326,806 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2559 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 3.9% G: 93.3% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 311.2 0 2 4 4 77.8 0 4 5 2 19.5 0 2 6 1 4.9 0 1 9 1121 0.1 0 45 1076 10 1080 0.0 1 39 1041 11 331 0.0 1 2 329 12 4 0.0 1 0 4 13 4 0.0 1 0 4 14 1 0.0 1 0 1 70 1 0.0 1 1 80 2 0.0 1 1 1 108 3 0.0 1 3 110 1 0.0 1 1 208 1 0.0 1 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.