This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.99 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 280,749 (10.6%) Reads that were too short: 16,531 (0.6%) Reads written (passing filters): 264,218 (9.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 75,402,450 bp (9.4%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 280749 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2358 649.0 0 2358 7 136836 649.0 0 136836 8 105714 649.0 0 105714 9 112 649.0 0 112 10 56 649.0 0 56 11 40 649.0 0 40 12 35 649.0 0 35 13 52 649.0 0 52 14 36 649.0 0 36 15 28 649.0 0 28 16 40 649.0 0 40 17 22 649.0 0 22 18 45 649.0 0 45 19 29 649.0 0 29 20 35 649.0 0 35 21 41 649.0 0 41 22 56 649.0 0 56 23 22 649.0 0 22 24 31 649.0 0 31 25 24 649.0 0 24 26 25 649.0 0 25 27 45 649.0 0 45 28 35 649.0 0 35 29 252 649.0 0 252 30 41 649.0 0 41 31 290 649.0 0 290 32 42 649.0 0 42 33 37 649.0 0 37 34 37 649.0 0 37 35 45 649.0 0 45 36 38 649.0 0 38 37 44 649.0 0 44 38 33 649.0 0 33 39 34 649.0 0 34 40 40 649.0 0 40 41 40 649.0 0 40 42 17 649.0 0 17 43 25 649.0 0 25 44 32 649.0 0 32 45 40 649.0 0 40 46 43 649.0 0 43 47 35 649.0 0 35 48 42 649.0 0 42 49 180 649.0 0 180 50 34 649.0 0 34 51 27 649.0 0 27 52 66 649.0 0 66 53 29 649.0 0 29 54 58 649.0 0 58 55 132 649.0 0 132 56 216 649.0 0 216 57 37 649.0 0 37 58 40 649.0 0 40 59 56 649.0 0 56 60 59 649.0 0 59 61 626 649.0 0 626 62 196 649.0 0 196 63 563 649.0 0 563 64 41 649.0 0 41 65 38 649.0 0 38 66 27 649.0 0 27 67 98 649.0 0 98 68 129 649.0 0 129 69 91 649.0 0 91 70 37 649.0 0 37 71 59 649.0 0 59 72 47 649.0 0 47 73 380 649.0 0 380 74 112 649.0 0 112 75 69 649.0 0 69 76 68 649.0 0 68 77 29 649.0 0 29 78 69 649.0 0 69 79 83 649.0 0 83 80 78 649.0 0 78 81 72 649.0 0 72 82 89 649.0 0 89 83 115 649.0 0 115 84 95 649.0 0 95 85 167 649.0 0 167 86 206 649.0 0 206 87 151 649.0 0 151 88 193 649.0 0 193 89 88 649.0 0 88 90 52 649.0 0 52 91 45 649.0 0 45 92 61 649.0 0 61 93 32 649.0 0 32 94 76 649.0 0 76 95 60 649.0 0 60 96 55 649.0 0 55 97 76 649.0 0 76 98 68 649.0 0 68 99 49 649.0 0 49 100 43 649.0 0 43 101 44 649.0 0 44 102 28 649.0 0 28 103 29 649.0 0 29 104 23 649.0 0 23 105 25 649.0 0 25 106 24 649.0 0 24 107 19 649.0 0 19 108 33 649.0 0 33 109 33 649.0 0 33 110 30 649.0 0 30 111 43 649.0 0 43 112 92 649.0 0 92 113 72 649.0 0 72 114 75 649.0 0 75 115 49 649.0 0 49 116 47 649.0 0 47 117 44 649.0 0 44 118 61 649.0 0 61 119 65 649.0 0 65 120 68 649.0 0 68 121 76 649.0 0 76 122 58 649.0 0 58 123 134 649.0 0 134 124 135 649.0 0 135 125 256 649.0 0 256 126 208 649.0 0 208 127 241 649.0 0 241 128 605 649.0 0 605 129 477 649.0 0 477 130 473 649.0 0 473 131 109 649.0 0 109 132 68 649.0 0 68 133 90 649.0 0 90 134 282 649.0 0 282 135 128 649.0 0 128 136 195 649.0 0 195 137 72 649.0 0 72 138 66 649.0 0 66 139 71 649.0 0 71 140 79 649.0 0 79 141 65 649.0 0 65 142 50 649.0 0 50 143 73 649.0 0 73 144 70 649.0 0 70 145 95 649.0 0 95 146 110 649.0 0 110 147 79 649.0 0 79 148 78 649.0 0 78 149 72 649.0 0 72 150 97 649.0 0 97 151 71 649.0 0 71 152 85 649.0 0 85 153 59 649.0 0 59 154 51 649.0 0 51 155 64 649.0 0 64 156 91 649.0 0 91 157 39 649.0 0 39 158 141 649.0 0 141 159 54 649.0 0 54 160 52 649.0 0 52 161 51 649.0 0 51 162 59 649.0 0 59 163 51 649.0 0 51 164 60 649.0 0 60 165 86 649.0 0 86 166 305 649.0 0 305 167 133 649.0 0 133 168 132 649.0 0 132 169 44 649.0 0 44 170 44 649.0 0 44 171 57 649.0 0 57 172 54 649.0 0 54 173 59 649.0 0 59 174 100 649.0 0 100 175 74 649.0 0 74 176 97 649.0 0 97 177 86 649.0 0 86 178 71 649.0 0 71 179 51 649.0 0 51 180 59 649.0 0 59 181 45 649.0 0 45 182 65 649.0 0 65 183 121 649.0 0 121 184 73 649.0 0 73 185 107 649.0 0 107 186 78 649.0 0 78 187 77 649.0 0 77 188 104 649.0 0 104 189 64 649.0 0 64 190 70 649.0 0 70 191 88 649.0 0 88 192 85 649.0 0 85 193 103 649.0 0 103 194 116 649.0 0 116 195 172 649.0 0 172 196 267 649.0 0 267 197 524 649.0 0 524 198 895 649.0 0 895 199 606 649.0 0 606 200 101 649.0 0 101 201 70 649.0 0 70 202 49 649.0 0 49 203 43 649.0 0 43 204 60 649.0 0 60 205 81 649.0 0 81 206 78 649.0 0 78 207 87 649.0 0 87 208 104 649.0 0 104 209 91 649.0 0 91 210 119 649.0 0 119 211 191 649.0 0 191 212 111 649.0 0 111 213 174 649.0 0 174 214 93 649.0 0 93 215 87 649.0 0 87 216 71 649.0 0 71 217 88 649.0 0 88 218 165 649.0 0 165 219 222 649.0 0 222 220 585 649.0 0 585 221 356 649.0 0 356 222 851 649.0 0 851 223 125 649.0 0 125 224 447 649.0 0 447 225 105 649.0 0 105 226 63 649.0 0 63 227 66 649.0 0 66 228 72 649.0 0 72 229 69 649.0 0 69 230 105 649.0 0 105 231 126 649.0 0 126 232 150 649.0 0 150 233 122 649.0 0 122 234 90 649.0 0 90 235 98 649.0 0 98 236 81 649.0 0 81 237 82 649.0 0 82 238 172 649.0 0 172 239 151 649.0 0 151 240 351 649.0 0 351 241 203 649.0 0 203 242 241 649.0 0 241 243 144 649.0 0 144 244 173 649.0 0 173 245 157 649.0 0 157 246 180 649.0 0 180 247 194 649.0 0 194 248 145 649.0 0 145 249 166 649.0 0 166 250 126 649.0 0 126 251 121 649.0 0 121 252 89 649.0 0 89 253 85 649.0 0 85 254 144 649.0 0 144 255 140 649.0 0 140 256 165 649.0 0 165 257 89 649.0 0 89 258 85 649.0 0 85 259 88 649.0 0 88 260 89 649.0 0 89 261 106 649.0 0 106 262 230 649.0 0 230 263 220 649.0 0 220 264 407 649.0 0 407 265 104 649.0 0 104 266 134 649.0 0 134 267 556 649.0 0 556 268 553 649.0 0 553 269 718 649.0 0 718 270 429 649.0 0 429 271 70 649.0 0 70 272 105 649.0 0 105 273 221 649.0 0 221 274 449 649.0 0 449 275 272 649.0 0 272 276 313 649.0 0 313 277 151 649.0 0 151 278 75 649.0 0 75 279 87 649.0 0 87 280 110 649.0 0 110 281 159 649.0 0 159 282 146 649.0 0 146 283 134 649.0 0 134 284 62 649.0 0 62 285 63 649.0 0 63 286 72 649.0 0 72 287 101 649.0 0 101 288 151 649.0 0 151 289 156 649.0 0 156 290 146 649.0 0 146 291 82 649.0 0 82 292 67 649.0 0 67 293 87 649.0 0 87 294 116 649.0 0 116 295 121 649.0 0 121 296 126 649.0 0 126 297 93 649.0 0 93 298 75 649.0 0 75 299 85 649.0 0 85 300 94 649.0 0 94 301 80 649.0 0 80 Finished in 11.87 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 264,218 Reads with adapters: 132,879 (50.3%) Reads that were too short: 104 (0.0%) Reads written (passing filters): 132,775 (50.3%) Total basepairs processed: 75,402,450 bp Total written (filtered): 36,759,785 bp (48.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 132879 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 65 0.0 3 0 0 37 28 16 1209 0.0 3 0 1034 114 61 17 130465 0.0 3 123604 5737 922 202 18 258 0.0 3 13 214 19 12 19 334 0.0 3 4 0 208 122 20 307 0.0 3 9 0 1 297 21 1 0.0 3 0 0 0 1 22 2 0.0 3 1 0 0 1 26 2 0.0 3 1 0 0 1 29 1 0.0 3 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 38 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 42 8 0.0 3 0 0 0 8 45 2 0.0 3 0 0 0 2 48 3 0.0 3 0 0 0 3 50 4 0.0 3 0 0 0 4 51 3 0.0 3 0 0 1 2 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 63 25 0.0 3 0 0 0 25 64 1 0.0 3 0 0 0 1 65 4 0.0 3 0 0 0 4 66 16 0.0 3 0 0 1 15 67 1 0.0 3 1 68 4 0.0 3 1 0 0 3 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 1 2 142 3 0.0 3 0 0 0 3 144 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 164 10 0.0 3 0 0 0 10 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 1 187 2 0.0 3 0 0 0 2 191 4 0.0 3 0 0 0 4 192 5 0.0 3 0 0 0 5 194 4 0.0 3 0 0 0 4 205 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 7 0.0 3 0 0 0 7 227 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 279 7 0.0 3 0 0 0 7 280 11 0.0 3 0 0 1 10 281 17 0.0 3 0 0 1 16 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.67 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 132,775 Reads with adapters: 2,812 (2.1%) Reads written (passing filters): 132,775 (100.0%) Total basepairs processed: 36,759,785 bp Total written (filtered): 36,751,183 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2812 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.3% C: 17.9% G: 46.6% T: 26.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2670 2074.6 0 2670 4 131 518.7 0 131 5 6 129.7 0 6 6 4 32.4 0 4 14 1 0.0 1 0 1