This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.69 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 279,558 (10.5%) Reads that were too short: 12,226 (0.5%) Reads written (passing filters): 267,332 (10.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 76,512,126 bp (9.6%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 279558 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1573 649.0 0 1573 7 117984 649.0 0 117984 8 131212 649.0 0 131212 9 164 649.0 0 164 10 34 649.0 0 34 11 34 649.0 0 34 12 26 649.0 0 26 13 24 649.0 0 24 14 26 649.0 0 26 15 32 649.0 0 32 16 29 649.0 0 29 17 36 649.0 0 36 18 35 649.0 0 35 19 27 649.0 0 27 20 35 649.0 0 35 21 37 649.0 0 37 22 31 649.0 0 31 23 22 649.0 0 22 24 17 649.0 0 17 25 19 649.0 0 19 26 23 649.0 0 23 27 26 649.0 0 26 28 22 649.0 0 22 29 31 649.0 0 31 30 21 649.0 0 21 31 29 649.0 0 29 32 35 649.0 0 35 33 15 649.0 0 15 34 43 649.0 0 43 35 39 649.0 0 39 36 47 649.0 0 47 37 33 649.0 0 33 38 40 649.0 0 40 39 37 649.0 0 37 40 40 649.0 0 40 41 29 649.0 0 29 42 28 649.0 0 28 43 20 649.0 0 20 44 44 649.0 0 44 45 40 649.0 0 40 46 47 649.0 0 47 47 23 649.0 0 23 48 33 649.0 0 33 49 181 649.0 0 181 50 27 649.0 0 27 51 33 649.0 0 33 52 70 649.0 0 70 53 31 649.0 0 31 54 46 649.0 0 46 55 126 649.0 0 126 56 213 649.0 0 213 57 23 649.0 0 23 58 33 649.0 0 33 59 63 649.0 0 63 60 54 649.0 0 54 61 718 649.0 0 718 62 251 649.0 0 251 63 677 649.0 0 677 64 51 649.0 0 51 65 38 649.0 0 38 66 43 649.0 0 43 67 73 649.0 0 73 68 104 649.0 0 104 69 83 649.0 0 83 70 21 649.0 0 21 71 43 649.0 0 43 72 36 649.0 0 36 73 37 649.0 0 37 74 35 649.0 0 35 75 25 649.0 0 25 76 44 649.0 0 44 77 18 649.0 0 18 78 60 649.0 0 60 79 51 649.0 0 51 80 62 649.0 0 62 81 50 649.0 0 50 82 34 649.0 0 34 83 44 649.0 0 44 84 42 649.0 0 42 85 46 649.0 0 46 86 61 649.0 0 61 87 107 649.0 0 107 88 74 649.0 0 74 89 63 649.0 0 63 90 57 649.0 0 57 91 45 649.0 0 45 92 46 649.0 0 46 93 50 649.0 0 50 94 57 649.0 0 57 95 66 649.0 0 66 96 76 649.0 0 76 97 71 649.0 0 71 98 96 649.0 0 96 99 84 649.0 0 84 100 60 649.0 0 60 101 52 649.0 0 52 102 51 649.0 0 51 103 47 649.0 0 47 104 31 649.0 0 31 105 21 649.0 0 21 106 40 649.0 0 40 107 30 649.0 0 30 108 41 649.0 0 41 109 56 649.0 0 56 110 35 649.0 0 35 111 46 649.0 0 46 112 60 649.0 0 60 113 46 649.0 0 46 114 61 649.0 0 61 115 66 649.0 0 66 116 39 649.0 0 39 117 40 649.0 0 40 118 59 649.0 0 59 119 51 649.0 0 51 120 56 649.0 0 56 121 58 649.0 0 58 122 59 649.0 0 59 123 122 649.0 0 122 124 105 649.0 0 105 125 192 649.0 0 192 126 172 649.0 0 172 127 212 649.0 0 212 128 442 649.0 0 442 129 256 649.0 0 256 130 255 649.0 0 255 131 59 649.0 0 59 132 55 649.0 0 55 133 60 649.0 0 60 134 274 649.0 0 274 135 124 649.0 0 124 136 215 649.0 0 215 137 58 649.0 0 58 138 49 649.0 0 49 139 61 649.0 0 61 140 50 649.0 0 50 141 58 649.0 0 58 142 37 649.0 0 37 143 59 649.0 0 59 144 49 649.0 0 49 145 87 649.0 0 87 146 89 649.0 0 89 147 54 649.0 0 54 148 80 649.0 0 80 149 59 649.0 0 59 150 65 649.0 0 65 151 62 649.0 0 62 152 76 649.0 0 76 153 45 649.0 0 45 154 33 649.0 0 33 155 43 649.0 0 43 156 83 649.0 0 83 157 50 649.0 0 50 158 132 649.0 0 132 159 49 649.0 0 49 160 45 649.0 0 45 161 50 649.0 0 50 162 72 649.0 0 72 163 55 649.0 0 55 164 49 649.0 0 49 165 70 649.0 0 70 166 173 649.0 0 173 167 80 649.0 0 80 168 131 649.0 0 131 169 47 649.0 0 47 170 43 649.0 0 43 171 45 649.0 0 45 172 56 649.0 0 56 173 50 649.0 0 50 174 81 649.0 0 81 175 53 649.0 0 53 176 76 649.0 0 76 177 95 649.0 0 95 178 60 649.0 0 60 179 59 649.0 0 59 180 44 649.0 0 44 181 61 649.0 0 61 182 63 649.0 0 63 183 114 649.0 0 114 184 68 649.0 0 68 185 89 649.0 0 89 186 82 649.0 0 82 187 79 649.0 0 79 188 62 649.0 0 62 189 60 649.0 0 60 190 61 649.0 0 61 191 70 649.0 0 70 192 56 649.0 0 56 193 105 649.0 0 105 194 94 649.0 0 94 195 156 649.0 0 156 196 246 649.0 0 246 197 598 649.0 0 598 198 1043 649.0 0 1043 199 855 649.0 0 855 200 122 649.0 0 122 201 57 649.0 0 57 202 55 649.0 0 55 203 46 649.0 0 46 204 60 649.0 0 60 205 60 649.0 0 60 206 59 649.0 0 59 207 63 649.0 0 63 208 67 649.0 0 67 209 79 649.0 0 79 210 82 649.0 0 82 211 94 649.0 0 94 212 99 649.0 0 99 213 130 649.0 0 130 214 105 649.0 0 105 215 82 649.0 0 82 216 70 649.0 0 70 217 71 649.0 0 71 218 119 649.0 0 119 219 163 649.0 0 163 220 355 649.0 0 355 221 302 649.0 0 302 222 714 649.0 0 714 223 108 649.0 0 108 224 510 649.0 0 510 225 94 649.0 0 94 226 74 649.0 0 74 227 49 649.0 0 49 228 68 649.0 0 68 229 66 649.0 0 66 230 95 649.0 0 95 231 88 649.0 0 88 232 100 649.0 0 100 233 80 649.0 0 80 234 74 649.0 0 74 235 79 649.0 0 79 236 100 649.0 0 100 237 71 649.0 0 71 238 174 649.0 0 174 239 153 649.0 0 153 240 301 649.0 0 301 241 135 649.0 0 135 242 155 649.0 0 155 243 115 649.0 0 115 244 117 649.0 0 117 245 90 649.0 0 90 246 113 649.0 0 113 247 113 649.0 0 113 248 125 649.0 0 125 249 93 649.0 0 93 250 83 649.0 0 83 251 80 649.0 0 80 252 67 649.0 0 67 253 63 649.0 0 63 254 119 649.0 0 119 255 131 649.0 0 131 256 180 649.0 0 180 257 78 649.0 0 78 258 79 649.0 0 79 259 69 649.0 0 69 260 75 649.0 0 75 261 81 649.0 0 81 262 204 649.0 0 204 263 300 649.0 0 300 264 670 649.0 0 670 265 61 649.0 0 61 266 92 649.0 0 92 267 225 649.0 0 225 268 207 649.0 0 207 269 254 649.0 0 254 270 191 649.0 0 191 271 58 649.0 0 58 272 64 649.0 0 64 273 120 649.0 0 120 274 256 649.0 0 256 275 142 649.0 0 142 276 113 649.0 0 113 277 82 649.0 0 82 278 60 649.0 0 60 279 81 649.0 0 81 280 67 649.0 0 67 281 120 649.0 0 120 282 79 649.0 0 79 283 106 649.0 0 106 284 55 649.0 0 55 285 37 649.0 0 37 286 64 649.0 0 64 287 83 649.0 0 83 288 94 649.0 0 94 289 102 649.0 0 102 290 84 649.0 0 84 291 60 649.0 0 60 292 59 649.0 0 59 293 64 649.0 0 64 294 71 649.0 0 71 295 112 649.0 0 112 296 70 649.0 0 70 297 74 649.0 0 74 298 66 649.0 0 66 299 66 649.0 0 66 300 100 649.0 0 100 301 93 649.0 0 93 Finished in 13.00 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 267,332 Reads with adapters: 137,788 (51.5%) Reads that were too short: 60 (0.0%) Reads written (passing filters): 137,728 (51.5%) Total basepairs processed: 76,512,126 bp Total written (filtered): 37,476,125 bp (49.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 137788 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 43 0.0 3 0 0 43 19 1251 0.0 3 0 1219 30 2 20 134729 0.0 4 132370 2275 73 9 2 21 304 0.0 4 31 271 1 1 22 27 0.0 4 5 2 19 0 1 23 148 0.0 4 1 1 0 126 20 24 6 0.0 4 2 0 0 0 4 25 1 0.0 4 0 0 0 1 26 2 0.0 4 1 1 32 2 0.0 4 0 1 0 0 1 35 1 0.0 4 1 40 1 0.0 4 1 41 1 0.0 4 1 68 1 0.0 4 1 71 2 0.0 4 2 83 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 2 0.0 4 1 0 0 0 1 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 10 0.0 4 0 0 0 0 10 127 1176 0.0 4 0 0 0 0 1176 128 4 0.0 4 0 0 0 0 4 129 2 0.0 4 0 0 0 0 2 130 7 0.0 4 0 0 0 0 7 148 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 162 1 0.0 4 1 194 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 15 0.0 4 0 0 0 0 15 229 9 0.0 4 0 0 0 1 8 230 5 0.0 4 0 0 0 0 5 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 4 0.0 4 0 0 0 0 4 236 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 250 2 0.0 4 0 0 0 0 2 256 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.75 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 137,728 Reads with adapters: 8,611 (6.3%) Reads written (passing filters): 137,728 (100.0%) Total basepairs processed: 37,476,125 bp Total written (filtered): 37,372,792 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8611 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 3.4% G: 92.1% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 101 2152.0 0 101 4 11 538.0 0 11 5 9 134.5 0 9 6 2 33.6 0 2 8 1 2.1 0 1 9 35 0.5 0 2 33 10 1428 0.1 1 42 1386 11 6941 0.0 1 50 6891 12 15 0.0 1 0 15 14 5 0.0 1 0 5 15 4 0.0 1 0 4 19 1 0.0 1 1 21 1 0.0 1 1 83 1 0.0 1 1 149 1 0.0 1 1 151 5 0.0 1 0 5 163 1 0.0 1 1 165 1 0.0 1 1 170 4 0.0 1 4 190 1 0.0 1 1 209 13 0.0 1 5 8 224 27 0.0 1 25 2 225 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.