This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.18 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 64,799 (2.4%) Reads that were too short: 29,902 (1.1%) Reads written (passing filters): 34,897 (1.3%) Total basepairs processed: 800,111,879 bp Total written (filtered): 6,995,424 bp (0.9%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 64799 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 179 649.0 0 179 7 8838 649.0 0 8838 8 119 649.0 0 119 9 990 649.0 0 990 10 16 649.0 0 16 11 12 649.0 0 12 12 21 649.0 0 21 13 9 649.0 0 9 14 18 649.0 0 18 15 9 649.0 0 9 16 23 649.0 0 23 17 8 649.0 0 8 18 10 649.0 0 10 19 7 649.0 0 7 20 8 649.0 0 8 21 12 649.0 0 12 22 8 649.0 0 8 23 36 649.0 0 36 24 18 649.0 0 18 25 11 649.0 0 11 26 10 649.0 0 10 27 8 649.0 0 8 28 3 649.0 0 3 29 18 649.0 0 18 30 12 649.0 0 12 31 13 649.0 0 13 32 18 649.0 0 18 33 17 649.0 0 17 34 12 649.0 0 12 35 12 649.0 0 12 36 39 649.0 0 39 37 12 649.0 0 12 38 21 649.0 0 21 39 22 649.0 0 22 40 22 649.0 0 22 41 112 649.0 0 112 42 34 649.0 0 34 43 34 649.0 0 34 44 18 649.0 0 18 45 14 649.0 0 14 46 10 649.0 0 10 47 16 649.0 0 16 48 13 649.0 0 13 49 12 649.0 0 12 50 24 649.0 0 24 51 55 649.0 0 55 52 29 649.0 0 29 53 37 649.0 0 37 54 17 649.0 0 17 55 40 649.0 0 40 56 21 649.0 0 21 57 23 649.0 0 23 58 13 649.0 0 13 59 13 649.0 0 13 60 16 649.0 0 16 61 14 649.0 0 14 62 10 649.0 0 10 63 16 649.0 0 16 64 13 649.0 0 13 65 11 649.0 0 11 66 26 649.0 0 26 67 28 649.0 0 28 68 28 649.0 0 28 69 125 649.0 0 125 70 82 649.0 0 82 71 133 649.0 0 133 72 22 649.0 0 22 73 25 649.0 0 25 74 29 649.0 0 29 75 41 649.0 0 41 76 46 649.0 0 46 77 55 649.0 0 55 78 45 649.0 0 45 79 23 649.0 0 23 80 93 649.0 0 93 81 50 649.0 0 50 82 49 649.0 0 49 83 50 649.0 0 50 84 34 649.0 0 34 85 37 649.0 0 37 86 52 649.0 0 52 87 119 649.0 0 119 88 57 649.0 0 57 89 141 649.0 0 141 90 206 649.0 0 206 91 114 649.0 0 114 92 84 649.0 0 84 93 94 649.0 0 94 94 49 649.0 0 49 95 42 649.0 0 42 96 72 649.0 0 72 97 84 649.0 0 84 98 91 649.0 0 91 99 69 649.0 0 69 100 601 649.0 0 601 101 886 649.0 0 886 102 2029 649.0 0 2029 103 490 649.0 0 490 104 100 649.0 0 100 105 39 649.0 0 39 106 34 649.0 0 34 107 33 649.0 0 33 108 37 649.0 0 37 109 37 649.0 0 37 110 50 649.0 0 50 111 38 649.0 0 38 112 42 649.0 0 42 113 54 649.0 0 54 114 41 649.0 0 41 115 60 649.0 0 60 116 92 649.0 0 92 117 60 649.0 0 60 118 120 649.0 0 120 119 77 649.0 0 77 120 103 649.0 0 103 121 78 649.0 0 78 122 112 649.0 0 112 123 150 649.0 0 150 124 143 649.0 0 143 125 97 649.0 0 97 126 81 649.0 0 81 127 64 649.0 0 64 128 80 649.0 0 80 129 87 649.0 0 87 130 123 649.0 0 123 131 280 649.0 0 280 132 463 649.0 0 463 133 310 649.0 0 310 134 204 649.0 0 204 135 98 649.0 0 98 136 114 649.0 0 114 137 144 649.0 0 144 138 138 649.0 0 138 139 107 649.0 0 107 140 130 649.0 0 130 141 126 649.0 0 126 142 174 649.0 0 174 143 258 649.0 0 258 144 282 649.0 0 282 145 415 649.0 0 415 146 87 649.0 0 87 147 96 649.0 0 96 148 139 649.0 0 139 149 144 649.0 0 144 150 140 649.0 0 140 151 122 649.0 0 122 152 174 649.0 0 174 153 149 649.0 0 149 154 141 649.0 0 141 155 147 649.0 0 147 156 141 649.0 0 141 157 331 649.0 0 331 158 618 649.0 0 618 159 659 649.0 0 659 160 476 649.0 0 476 161 419 649.0 0 419 162 332 649.0 0 332 163 130 649.0 0 130 164 120 649.0 0 120 165 105 649.0 0 105 166 86 649.0 0 86 167 108 649.0 0 108 168 134 649.0 0 134 169 201 649.0 0 201 170 145 649.0 0 145 171 162 649.0 0 162 172 127 649.0 0 127 173 130 649.0 0 130 174 94 649.0 0 94 175 122 649.0 0 122 176 426 649.0 0 426 177 358 649.0 0 358 178 519 649.0 0 519 179 189 649.0 0 189 180 150 649.0 0 150 181 131 649.0 0 131 182 160 649.0 0 160 183 171 649.0 0 171 184 207 649.0 0 207 185 215 649.0 0 215 186 158 649.0 0 158 187 838 649.0 0 838 188 534 649.0 0 534 189 269 649.0 0 269 190 184 649.0 0 184 191 147 649.0 0 147 192 113 649.0 0 113 193 83 649.0 0 83 194 124 649.0 0 124 195 119 649.0 0 119 196 205 649.0 0 205 197 188 649.0 0 188 198 200 649.0 0 200 199 185 649.0 0 185 200 147 649.0 0 147 201 116 649.0 0 116 202 224 649.0 0 224 203 266 649.0 0 266 204 336 649.0 0 336 205 207 649.0 0 207 206 142 649.0 0 142 207 148 649.0 0 148 208 99 649.0 0 99 209 156 649.0 0 156 210 194 649.0 0 194 211 262 649.0 0 262 212 271 649.0 0 271 213 208 649.0 0 208 214 182 649.0 0 182 215 208 649.0 0 208 216 229 649.0 0 229 217 225 649.0 0 225 218 206 649.0 0 206 219 203 649.0 0 203 220 146 649.0 0 146 221 149 649.0 0 149 222 282 649.0 0 282 223 422 649.0 0 422 224 562 649.0 0 562 225 424 649.0 0 424 226 324 649.0 0 324 227 202 649.0 0 202 228 155 649.0 0 155 229 181 649.0 0 181 230 141 649.0 0 141 231 118 649.0 0 118 232 132 649.0 0 132 233 153 649.0 0 153 234 174 649.0 0 174 235 218 649.0 0 218 236 191 649.0 0 191 237 228 649.0 0 228 238 656 649.0 0 656 239 567 649.0 0 567 240 411 649.0 0 411 241 187 649.0 0 187 242 336 649.0 0 336 243 430 649.0 0 430 244 442 649.0 0 442 245 426 649.0 0 426 246 296 649.0 0 296 247 306 649.0 0 306 248 227 649.0 0 227 249 204 649.0 0 204 250 193 649.0 0 193 251 343 649.0 0 343 252 219 649.0 0 219 253 306 649.0 0 306 254 204 649.0 0 204 255 310 649.0 0 310 256 331 649.0 0 331 257 301 649.0 0 301 258 242 649.0 0 242 259 186 649.0 0 186 260 231 649.0 0 231 261 197 649.0 0 197 262 482 649.0 0 482 263 545 649.0 0 545 264 748 649.0 0 748 265 573 649.0 0 573 266 469 649.0 0 469 267 256 649.0 0 256 268 181 649.0 0 181 269 194 649.0 0 194 270 300 649.0 0 300 271 438 649.0 0 438 272 393 649.0 0 393 273 362 649.0 0 362 274 415 649.0 0 415 275 358 649.0 0 358 276 381 649.0 0 381 277 353 649.0 0 353 278 342 649.0 0 342 279 336 649.0 0 336 280 267 649.0 0 267 281 253 649.0 0 253 282 273 649.0 0 273 283 364 649.0 0 364 284 466 649.0 0 466 285 391 649.0 0 391 286 371 649.0 0 371 287 359 649.0 0 359 288 266 649.0 0 266 289 227 649.0 0 227 290 271 649.0 0 271 291 371 649.0 0 371 292 533 649.0 0 533 293 419 649.0 0 419 294 388 649.0 0 388 295 202 649.0 0 202 296 187 649.0 0 187 297 239 649.0 0 239 298 290 649.0 0 290 299 401 649.0 0 401 300 369 649.0 0 369 301 350 649.0 0 350 Finished in 1.43 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 34,897 Reads with adapters: 9,373 (26.9%) Reads that were too short: 129 (0.4%) Reads written (passing filters): 9,244 (26.5%) Total basepairs processed: 6,995,424 bp Total written (filtered): 2,507,270 bp (35.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9373 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 0 2 16 11 0.0 3 0 5 0 6 17 999 0.0 3 926 59 10 4 18 92 0.0 3 4 75 8 5 19 7830 0.0 3 7338 390 86 16 20 30 0.0 3 4 22 3 1 21 15 0.0 3 0 0 7 8 22 53 0.0 3 0 0 0 53 23 5 0.0 3 0 0 0 5 24 2 0.0 3 0 0 0 2 25 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 2 0.0 3 1 0 0 1 30 6 0.0 3 0 0 0 6 33 2 0.0 3 0 0 1 1 34 20 0.0 3 0 0 0 20 35 2 0.0 3 0 0 0 2 37 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 18 0.0 3 0 0 0 18 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 8 0.0 3 0 0 1 7 56 1 0.0 3 0 0 1 57 3 0.0 3 0 0 0 3 58 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 62 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 3 0.0 3 0 0 0 3 67 5 0.0 3 0 0 0 5 69 1 0.0 3 0 0 0 1 71 35 0.0 3 0 0 1 34 72 18 0.0 3 0 0 0 18 73 12 0.0 3 0 0 1 11 74 7 0.0 3 0 0 0 7 75 4 0.0 3 0 0 1 3 76 3 0.0 3 0 0 0 3 77 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 86 3 0.0 3 0 0 0 3 87 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 9 0.0 3 0 0 0 9 92 5 0.0 3 0 0 0 5 93 3 0.0 3 0 0 0 3 94 7 0.0 3 0 0 1 6 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 5 0.0 3 0 0 0 5 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 1 108 4 0.0 3 0 0 0 4 109 3 0.0 3 0 0 0 3 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 124 4 0.0 3 0 0 0 4 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 135 3 0.0 3 0 0 0 3 136 3 0.0 3 0 0 0 3 138 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 152 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 1 155 2 0.0 3 0 0 1 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 4 0.0 3 0 0 0 4 185 4 0.0 3 0 0 0 4 186 1 0.0 3 0 0 0 1 187 7 0.0 3 0 0 1 6 188 3 0.0 3 0 0 0 3 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 9,244 Reads with adapters: 218 (2.4%) Reads written (passing filters): 9,244 (100.0%) Total basepairs processed: 2,507,270 bp Total written (filtered): 2,506,600 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 218 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.3% C: 19.3% G: 51.8% T: 4.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 202 144.4 0 202 4 16 36.1 0 16