This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.90 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 42,347 (1.6%) Reads that were too short: 16,694 (0.6%) Reads written (passing filters): 25,653 (1.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 5,454,546 bp (0.7%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 42347 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 141 649.0 0 141 7 8672 649.0 0 8672 8 99 649.0 0 99 9 904 649.0 0 904 10 11 649.0 0 11 11 14 649.0 0 14 12 14 649.0 0 14 13 19 649.0 0 19 14 12 649.0 0 12 15 161 649.0 0 161 16 26 649.0 0 26 17 10 649.0 0 10 18 11 649.0 0 11 19 5 649.0 0 5 20 15 649.0 0 15 21 14 649.0 0 14 22 14 649.0 0 14 23 23 649.0 0 23 24 16 649.0 0 16 25 12 649.0 0 12 26 10 649.0 0 10 27 7 649.0 0 7 28 12 649.0 0 12 29 21 649.0 0 21 30 21 649.0 0 21 31 10 649.0 0 10 32 18 649.0 0 18 33 25 649.0 0 25 34 31 649.0 0 31 35 16 649.0 0 16 36 27 649.0 0 27 37 9 649.0 0 9 38 14 649.0 0 14 39 28 649.0 0 28 40 14 649.0 0 14 41 15 649.0 0 15 42 27 649.0 0 27 43 17 649.0 0 17 44 24 649.0 0 24 45 14 649.0 0 14 46 11 649.0 0 11 47 18 649.0 0 18 48 13 649.0 0 13 49 8 649.0 0 8 50 25 649.0 0 25 51 47 649.0 0 47 52 22 649.0 0 22 53 14 649.0 0 14 54 17 649.0 0 17 55 23 649.0 0 23 56 12 649.0 0 12 57 12 649.0 0 12 58 13 649.0 0 13 59 9 649.0 0 9 60 25 649.0 0 25 61 14 649.0 0 14 62 13 649.0 0 13 63 13 649.0 0 13 64 20 649.0 0 20 65 19 649.0 0 19 66 22 649.0 0 22 67 16 649.0 0 16 68 24 649.0 0 24 69 104 649.0 0 104 70 51 649.0 0 51 71 115 649.0 0 115 72 35 649.0 0 35 73 20 649.0 0 20 74 22 649.0 0 22 75 9 649.0 0 9 76 15 649.0 0 15 77 19 649.0 0 19 78 23 649.0 0 23 79 17 649.0 0 17 80 24 649.0 0 24 81 32 649.0 0 32 82 53 649.0 0 53 83 26 649.0 0 26 84 45 649.0 0 45 85 26 649.0 0 26 86 33 649.0 0 33 87 78 649.0 0 78 88 38 649.0 0 38 89 49 649.0 0 49 90 86 649.0 0 86 91 83 649.0 0 83 92 44 649.0 0 44 93 50 649.0 0 50 94 42 649.0 0 42 95 34 649.0 0 34 96 35 649.0 0 35 97 39 649.0 0 39 98 50 649.0 0 50 99 47 649.0 0 47 100 245 649.0 0 245 101 321 649.0 0 321 102 804 649.0 0 804 103 360 649.0 0 360 104 75 649.0 0 75 105 47 649.0 0 47 106 50 649.0 0 50 107 45 649.0 0 45 108 31 649.0 0 31 109 31 649.0 0 31 110 51 649.0 0 51 111 44 649.0 0 44 112 40 649.0 0 40 113 42 649.0 0 42 114 35 649.0 0 35 115 32 649.0 0 32 116 69 649.0 0 69 117 36 649.0 0 36 118 97 649.0 0 97 119 49 649.0 0 49 120 59 649.0 0 59 121 50 649.0 0 50 122 67 649.0 0 67 123 93 649.0 0 93 124 128 649.0 0 128 125 64 649.0 0 64 126 47 649.0 0 47 127 32 649.0 0 32 128 45 649.0 0 45 129 45 649.0 0 45 130 91 649.0 0 91 131 204 649.0 0 204 132 272 649.0 0 272 133 179 649.0 0 179 134 139 649.0 0 139 135 65 649.0 0 65 136 75 649.0 0 75 137 79 649.0 0 79 138 85 649.0 0 85 139 79 649.0 0 79 140 95 649.0 0 95 141 87 649.0 0 87 142 109 649.0 0 109 143 137 649.0 0 137 144 189 649.0 0 189 145 228 649.0 0 228 146 63 649.0 0 63 147 64 649.0 0 64 148 82 649.0 0 82 149 98 649.0 0 98 150 73 649.0 0 73 151 76 649.0 0 76 152 112 649.0 0 112 153 87 649.0 0 87 154 73 649.0 0 73 155 74 649.0 0 74 156 91 649.0 0 91 157 189 649.0 0 189 158 421 649.0 0 421 159 553 649.0 0 553 160 271 649.0 0 271 161 221 649.0 0 221 162 202 649.0 0 202 163 61 649.0 0 61 164 63 649.0 0 63 165 56 649.0 0 56 166 42 649.0 0 42 167 51 649.0 0 51 168 104 649.0 0 104 169 99 649.0 0 99 170 125 649.0 0 125 171 92 649.0 0 92 172 86 649.0 0 86 173 87 649.0 0 87 174 72 649.0 0 72 175 72 649.0 0 72 176 378 649.0 0 378 177 253 649.0 0 253 178 362 649.0 0 362 179 121 649.0 0 121 180 90 649.0 0 90 181 73 649.0 0 73 182 78 649.0 0 78 183 90 649.0 0 90 184 101 649.0 0 101 185 124 649.0 0 124 186 105 649.0 0 105 187 866 649.0 0 866 188 459 649.0 0 459 189 183 649.0 0 183 190 114 649.0 0 114 191 102 649.0 0 102 192 61 649.0 0 61 193 50 649.0 0 50 194 62 649.0 0 62 195 75 649.0 0 75 196 106 649.0 0 106 197 118 649.0 0 118 198 136 649.0 0 136 199 84 649.0 0 84 200 72 649.0 0 72 201 76 649.0 0 76 202 97 649.0 0 97 203 122 649.0 0 122 204 213 649.0 0 213 205 81 649.0 0 81 206 74 649.0 0 74 207 84 649.0 0 84 208 84 649.0 0 84 209 92 649.0 0 92 210 128 649.0 0 128 211 163 649.0 0 163 212 164 649.0 0 164 213 130 649.0 0 130 214 93 649.0 0 93 215 107 649.0 0 107 216 132 649.0 0 132 217 118 649.0 0 118 218 97 649.0 0 97 219 101 649.0 0 101 220 87 649.0 0 87 221 95 649.0 0 95 222 120 649.0 0 120 223 148 649.0 0 148 224 221 649.0 0 221 225 191 649.0 0 191 226 188 649.0 0 188 227 100 649.0 0 100 228 103 649.0 0 103 229 106 649.0 0 106 230 99 649.0 0 99 231 92 649.0 0 92 232 82 649.0 0 82 233 92 649.0 0 92 234 118 649.0 0 118 235 133 649.0 0 133 236 122 649.0 0 122 237 137 649.0 0 137 238 439 649.0 0 439 239 466 649.0 0 466 240 306 649.0 0 306 241 141 649.0 0 141 242 151 649.0 0 151 243 197 649.0 0 197 244 162 649.0 0 162 245 201 649.0 0 201 246 187 649.0 0 187 247 165 649.0 0 165 248 159 649.0 0 159 249 137 649.0 0 137 250 135 649.0 0 135 251 179 649.0 0 179 252 147 649.0 0 147 253 150 649.0 0 150 254 129 649.0 0 129 255 155 649.0 0 155 256 172 649.0 0 172 257 137 649.0 0 137 258 141 649.0 0 141 259 125 649.0 0 125 260 139 649.0 0 139 261 126 649.0 0 126 262 310 649.0 0 310 263 348 649.0 0 348 264 416 649.0 0 416 265 278 649.0 0 278 266 235 649.0 0 235 267 134 649.0 0 134 268 103 649.0 0 103 269 107 649.0 0 107 270 146 649.0 0 146 271 174 649.0 0 174 272 209 649.0 0 209 273 170 649.0 0 170 274 240 649.0 0 240 275 201 649.0 0 201 276 213 649.0 0 213 277 191 649.0 0 191 278 174 649.0 0 174 279 146 649.0 0 146 280 160 649.0 0 160 281 136 649.0 0 136 282 171 649.0 0 171 283 188 649.0 0 188 284 221 649.0 0 221 285 203 649.0 0 203 286 174 649.0 0 174 287 186 649.0 0 186 288 153 649.0 0 153 289 101 649.0 0 101 290 154 649.0 0 154 291 166 649.0 0 166 292 268 649.0 0 268 293 229 649.0 0 229 294 214 649.0 0 214 295 157 649.0 0 157 296 112 649.0 0 112 297 160 649.0 0 160 298 188 649.0 0 188 299 278 649.0 0 278 300 280 649.0 0 280 301 240 649.0 0 240 Finished in 1.15 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 25,653 Reads with adapters: 1,242 (4.8%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 1,220 (4.8%) Total basepairs processed: 5,454,546 bp Total written (filtered): 322,853 bp (5.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 16 0.0 3 0 14 2 20 1084 0.0 4 1064 17 2 0 1 21 4 0.0 4 1 3 22 6 0.0 4 6 23 3 0.0 4 1 0 0 1 1 34 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 1 0 0 1 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 129 99 0.0 4 0 0 0 0 99 134 2 0.0 4 0 0 0 1 1 135 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 1 170 1 0.0 4 0 0 0 0 1 179 2 0.0 4 0 0 0 0 2 215 1 0.0 4 1 230 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (25 us/read; 2.44 M reads/minute). === Summary === Total reads processed: 1,220 Reads with adapters: 47 (3.9%) Reads written (passing filters): 1,220 (100.0%) Total basepairs processed: 322,853 bp Total written (filtered): 313,964 bp (97.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 47 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.5% C: 0.0% G: 10.6% T: 78.7% none/other: 2.1% Overview of removed sequences length count expect max.err error counts 3 3 19.1 0 3 11 4 0.0 1 0 4 12 1 0.0 1 0 1 224 1 0.0 1 0 1 225 36 0.0 1 34 2 226 1 0.0 1 1 274 1 0.0 1 1