This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.71 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 62,938 (2.4%) Reads that were too short: 42,373 (1.6%) Reads written (passing filters): 20,565 (0.8%) Total basepairs processed: 800,111,879 bp Total written (filtered): 4,361,719 bp (0.5%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 62938 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 112 649.0 0 112 7 123 649.0 0 123 8 2506 649.0 0 2506 9 2276 649.0 0 2276 10 58 649.0 0 58 11 51 649.0 0 51 12 62 649.0 0 62 13 65 649.0 0 65 14 48 649.0 0 48 15 42 649.0 0 42 16 32 649.0 0 32 17 50 649.0 0 50 18 35 649.0 0 35 19 66 649.0 0 66 20 44 649.0 0 44 21 34 649.0 0 34 22 41 649.0 0 41 23 36 649.0 0 36 24 56 649.0 0 56 25 40 649.0 0 40 26 33 649.0 0 33 27 33 649.0 0 33 28 33 649.0 0 33 29 37 649.0 0 37 30 42 649.0 0 42 31 56 649.0 0 56 32 43 649.0 0 43 33 47 649.0 0 47 34 48 649.0 0 48 35 50 649.0 0 50 36 72 649.0 0 72 37 64 649.0 0 64 38 46 649.0 0 46 39 40 649.0 0 40 40 77 649.0 0 77 41 64 649.0 0 64 42 56 649.0 0 56 43 44 649.0 0 44 44 45 649.0 0 45 45 51 649.0 0 51 46 59 649.0 0 59 47 45 649.0 0 45 48 52 649.0 0 52 49 50 649.0 0 50 50 59 649.0 0 59 51 159 649.0 0 159 52 120 649.0 0 120 53 245 649.0 0 245 54 40 649.0 0 40 55 44 649.0 0 44 56 34 649.0 0 34 57 38 649.0 0 38 58 67 649.0 0 67 59 57 649.0 0 57 60 48 649.0 0 48 61 55 649.0 0 55 62 138 649.0 0 138 63 65 649.0 0 65 64 159 649.0 0 159 65 43 649.0 0 43 66 76 649.0 0 76 67 60 649.0 0 60 68 64 649.0 0 64 69 44 649.0 0 44 70 38 649.0 0 38 71 63 649.0 0 63 72 43 649.0 0 43 73 52 649.0 0 52 74 35 649.0 0 35 75 51 649.0 0 51 76 85 649.0 0 85 77 55 649.0 0 55 78 90 649.0 0 90 79 68 649.0 0 68 80 73 649.0 0 73 81 143 649.0 0 143 82 202 649.0 0 202 83 54 649.0 0 54 84 46 649.0 0 46 85 80 649.0 0 80 86 46 649.0 0 46 87 51 649.0 0 51 88 58 649.0 0 58 89 80 649.0 0 80 90 78 649.0 0 78 91 95 649.0 0 95 92 111 649.0 0 111 93 262 649.0 0 262 94 115 649.0 0 115 95 122 649.0 0 122 96 77 649.0 0 77 97 75 649.0 0 75 98 72 649.0 0 72 99 71 649.0 0 71 100 102 649.0 0 102 101 137 649.0 0 137 102 113 649.0 0 113 103 66 649.0 0 66 104 78 649.0 0 78 105 93 649.0 0 93 106 61 649.0 0 61 107 61 649.0 0 61 108 44 649.0 0 44 109 54 649.0 0 54 110 50 649.0 0 50 111 45 649.0 0 45 112 57 649.0 0 57 113 63 649.0 0 63 114 54 649.0 0 54 115 71 649.0 0 71 116 65 649.0 0 65 117 91 649.0 0 91 118 91 649.0 0 91 119 80 649.0 0 80 120 55 649.0 0 55 121 51 649.0 0 51 122 68 649.0 0 68 123 63 649.0 0 63 124 73 649.0 0 73 125 49 649.0 0 49 126 57 649.0 0 57 127 83 649.0 0 83 128 58 649.0 0 58 129 64 649.0 0 64 130 71 649.0 0 71 131 81 649.0 0 81 132 116 649.0 0 116 133 86 649.0 0 86 134 59 649.0 0 59 135 43 649.0 0 43 136 97 649.0 0 97 137 355 649.0 0 355 138 583 649.0 0 583 139 748 649.0 0 748 140 331 649.0 0 331 141 53 649.0 0 53 142 72 649.0 0 72 143 99 649.0 0 99 144 104 649.0 0 104 145 104 649.0 0 104 146 120 649.0 0 120 147 100 649.0 0 100 148 102 649.0 0 102 149 58 649.0 0 58 150 62 649.0 0 62 151 82 649.0 0 82 152 70 649.0 0 70 153 75 649.0 0 75 154 77 649.0 0 77 155 64 649.0 0 64 156 68 649.0 0 68 157 60 649.0 0 60 158 71 649.0 0 71 159 75 649.0 0 75 160 66 649.0 0 66 161 65 649.0 0 65 162 61 649.0 0 61 163 65 649.0 0 65 164 47 649.0 0 47 165 66 649.0 0 66 166 60 649.0 0 60 167 55 649.0 0 55 168 53 649.0 0 53 169 81 649.0 0 81 170 57 649.0 0 57 171 73 649.0 0 73 172 72 649.0 0 72 173 71 649.0 0 71 174 59 649.0 0 59 175 46 649.0 0 46 176 80 649.0 0 80 177 69 649.0 0 69 178 112 649.0 0 112 179 99 649.0 0 99 180 79 649.0 0 79 181 144 649.0 0 144 182 71 649.0 0 71 183 70 649.0 0 70 184 89 649.0 0 89 185 68 649.0 0 68 186 165 649.0 0 165 187 146 649.0 0 146 188 162 649.0 0 162 189 172 649.0 0 172 190 57 649.0 0 57 191 49 649.0 0 49 192 77 649.0 0 77 193 48 649.0 0 48 194 52 649.0 0 52 195 68 649.0 0 68 196 60 649.0 0 60 197 94 649.0 0 94 198 92 649.0 0 92 199 73 649.0 0 73 200 53 649.0 0 53 201 66 649.0 0 66 202 59 649.0 0 59 203 75 649.0 0 75 204 88 649.0 0 88 205 113 649.0 0 113 206 74 649.0 0 74 207 80 649.0 0 80 208 189 649.0 0 189 209 58 649.0 0 58 210 80 649.0 0 80 211 80 649.0 0 80 212 80 649.0 0 80 213 64 649.0 0 64 214 235 649.0 0 235 215 165 649.0 0 165 216 256 649.0 0 256 217 171 649.0 0 171 218 92 649.0 0 92 219 68 649.0 0 68 220 53 649.0 0 53 221 68 649.0 0 68 222 60 649.0 0 60 223 81 649.0 0 81 224 97 649.0 0 97 225 81 649.0 0 81 226 92 649.0 0 92 227 58 649.0 0 58 228 92 649.0 0 92 229 48 649.0 0 48 230 74 649.0 0 74 231 75 649.0 0 75 232 50 649.0 0 50 233 69 649.0 0 69 234 64 649.0 0 64 235 84 649.0 0 84 236 75 649.0 0 75 237 72 649.0 0 72 238 138 649.0 0 138 239 97 649.0 0 97 240 159 649.0 0 159 241 63 649.0 0 63 242 64 649.0 0 64 243 82 649.0 0 82 244 87 649.0 0 87 245 80 649.0 0 80 246 63 649.0 0 63 247 65 649.0 0 65 248 86 649.0 0 86 249 78 649.0 0 78 250 114 649.0 0 114 251 111 649.0 0 111 252 131 649.0 0 131 253 82 649.0 0 82 254 70 649.0 0 70 255 81 649.0 0 81 256 112 649.0 0 112 257 164 649.0 0 164 258 184 649.0 0 184 259 139 649.0 0 139 260 129 649.0 0 129 261 100 649.0 0 100 262 128 649.0 0 128 263 124 649.0 0 124 264 122 649.0 0 122 265 100 649.0 0 100 266 140 649.0 0 140 267 97 649.0 0 97 268 105 649.0 0 105 269 96 649.0 0 96 270 144 649.0 0 144 271 178 649.0 0 178 272 356 649.0 0 356 273 1494 649.0 0 1494 274 1356 649.0 0 1356 275 3136 649.0 0 3136 276 266 649.0 0 266 277 359 649.0 0 359 278 292 649.0 0 292 279 198 649.0 0 198 280 229 649.0 0 229 281 125 649.0 0 125 282 264 649.0 0 264 283 1150 649.0 0 1150 284 769 649.0 0 769 285 1419 649.0 0 1419 286 1088 649.0 0 1088 287 722 649.0 0 722 288 597 649.0 0 597 289 190 649.0 0 190 290 208 649.0 0 208 291 195 649.0 0 195 292 335 649.0 0 335 293 2556 649.0 0 2556 294 2026 649.0 0 2026 295 1439 649.0 0 1439 296 340 649.0 0 340 297 815 649.0 0 815 298 2490 649.0 0 2490 299 5003 649.0 0 5003 300 4345 649.0 0 4345 301 1578 649.0 0 1578 Finished in 0.85 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 20,565 Reads with adapters: 2,614 (12.7%) Reads that were too short: 40 (0.2%) Reads written (passing filters): 2,574 (12.5%) Total basepairs processed: 4,361,719 bp Total written (filtered): 701,419 bp (16.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2614 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 25 0.0 3 0 23 2 17 2428 0.0 3 2274 130 17 7 18 16 0.0 3 4 8 3 1 19 12 0.0 3 2 1 6 3 20 23 0.0 3 14 2 1 6 21 1 0.0 3 1 24 5 0.0 3 0 0 0 5 27 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 3 0.0 3 1 0 0 2 36 2 0.0 3 1 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 1 2 48 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 1 1 54 2 0.0 3 0 0 0 2 55 9 0.0 3 0 0 0 9 57 2 0.0 3 0 0 0 2 58 4 0.0 3 0 0 0 4 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 76 6 0.0 3 0 0 0 6 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 1 104 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 2,574 Reads with adapters: 35 (1.4%) Reads written (passing filters): 2,574 (100.0%) Total basepairs processed: 701,419 bp Total written (filtered): 701,306 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.3% C: 17.1% G: 45.7% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 28 40.2 0 28 4 6 10.1 0 6 5 1 2.5 0 1