This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.54 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 61,427 (2.3%) Reads that were too short: 45,216 (1.7%) Reads written (passing filters): 16,211 (0.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 3,575,800 bp (0.4%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 61427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 56 649.0 0 56 7 88 649.0 0 88 8 2380 649.0 0 2380 9 2610 649.0 0 2610 10 49 649.0 0 49 11 27 649.0 0 27 12 30 649.0 0 30 13 58 649.0 0 58 14 28 649.0 0 28 15 18 649.0 0 18 16 37 649.0 0 37 17 33 649.0 0 33 18 30 649.0 0 30 19 54 649.0 0 54 20 39 649.0 0 39 21 38 649.0 0 38 22 28 649.0 0 28 23 21 649.0 0 21 24 47 649.0 0 47 25 28 649.0 0 28 26 28 649.0 0 28 27 32 649.0 0 32 28 42 649.0 0 42 29 30 649.0 0 30 30 31 649.0 0 31 31 44 649.0 0 44 32 42 649.0 0 42 33 40 649.0 0 40 34 37 649.0 0 37 35 53 649.0 0 53 36 55 649.0 0 55 37 45 649.0 0 45 38 55 649.0 0 55 39 36 649.0 0 36 40 54 649.0 0 54 41 42 649.0 0 42 42 31 649.0 0 31 43 27 649.0 0 27 44 53 649.0 0 53 45 40 649.0 0 40 46 49 649.0 0 49 47 35 649.0 0 35 48 27 649.0 0 27 49 31 649.0 0 31 50 54 649.0 0 54 51 114 649.0 0 114 52 56 649.0 0 56 53 74 649.0 0 74 54 36 649.0 0 36 55 58 649.0 0 58 56 30 649.0 0 30 57 26 649.0 0 26 58 39 649.0 0 39 59 33 649.0 0 33 60 48 649.0 0 48 61 49 649.0 0 49 62 91 649.0 0 91 63 36 649.0 0 36 64 126 649.0 0 126 65 48 649.0 0 48 66 50 649.0 0 50 67 59 649.0 0 59 68 67 649.0 0 67 69 54 649.0 0 54 70 40 649.0 0 40 71 38 649.0 0 38 72 35 649.0 0 35 73 41 649.0 0 41 74 51 649.0 0 51 75 45 649.0 0 45 76 34 649.0 0 34 77 41 649.0 0 41 78 44 649.0 0 44 79 52 649.0 0 52 80 44 649.0 0 44 81 98 649.0 0 98 82 196 649.0 0 196 83 29 649.0 0 29 84 35 649.0 0 35 85 51 649.0 0 51 86 38 649.0 0 38 87 34 649.0 0 34 88 30 649.0 0 30 89 55 649.0 0 55 90 55 649.0 0 55 91 49 649.0 0 49 92 94 649.0 0 94 93 215 649.0 0 215 94 78 649.0 0 78 95 53 649.0 0 53 96 72 649.0 0 72 97 66 649.0 0 66 98 49 649.0 0 49 99 74 649.0 0 74 100 98 649.0 0 98 101 86 649.0 0 86 102 101 649.0 0 101 103 45 649.0 0 45 104 45 649.0 0 45 105 62 649.0 0 62 106 37 649.0 0 37 107 50 649.0 0 50 108 43 649.0 0 43 109 63 649.0 0 63 110 51 649.0 0 51 111 31 649.0 0 31 112 41 649.0 0 41 113 43 649.0 0 43 114 50 649.0 0 50 115 90 649.0 0 90 116 31 649.0 0 31 117 63 649.0 0 63 118 64 649.0 0 64 119 63 649.0 0 63 120 41 649.0 0 41 121 39 649.0 0 39 122 43 649.0 0 43 123 45 649.0 0 45 124 48 649.0 0 48 125 42 649.0 0 42 126 33 649.0 0 33 127 92 649.0 0 92 128 55 649.0 0 55 129 48 649.0 0 48 130 63 649.0 0 63 131 56 649.0 0 56 132 51 649.0 0 51 133 42 649.0 0 42 134 44 649.0 0 44 135 38 649.0 0 38 136 58 649.0 0 58 137 131 649.0 0 131 138 346 649.0 0 346 139 354 649.0 0 354 140 217 649.0 0 217 141 38 649.0 0 38 142 45 649.0 0 45 143 47 649.0 0 47 144 76 649.0 0 76 145 58 649.0 0 58 146 60 649.0 0 60 147 59 649.0 0 59 148 56 649.0 0 56 149 39 649.0 0 39 150 35 649.0 0 35 151 67 649.0 0 67 152 49 649.0 0 49 153 48 649.0 0 48 154 49 649.0 0 49 155 48 649.0 0 48 156 40 649.0 0 40 157 53 649.0 0 53 158 53 649.0 0 53 159 50 649.0 0 50 160 38 649.0 0 38 161 40 649.0 0 40 162 49 649.0 0 49 163 49 649.0 0 49 164 47 649.0 0 47 165 59 649.0 0 59 166 50 649.0 0 50 167 47 649.0 0 47 168 43 649.0 0 43 169 40 649.0 0 40 170 48 649.0 0 48 171 72 649.0 0 72 172 53 649.0 0 53 173 56 649.0 0 56 174 51 649.0 0 51 175 44 649.0 0 44 176 64 649.0 0 64 177 51 649.0 0 51 178 110 649.0 0 110 179 83 649.0 0 83 180 58 649.0 0 58 181 128 649.0 0 128 182 42 649.0 0 42 183 59 649.0 0 59 184 79 649.0 0 79 185 73 649.0 0 73 186 102 649.0 0 102 187 88 649.0 0 88 188 111 649.0 0 111 189 82 649.0 0 82 190 47 649.0 0 47 191 59 649.0 0 59 192 47 649.0 0 47 193 36 649.0 0 36 194 56 649.0 0 56 195 60 649.0 0 60 196 51 649.0 0 51 197 65 649.0 0 65 198 68 649.0 0 68 199 44 649.0 0 44 200 53 649.0 0 53 201 54 649.0 0 54 202 53 649.0 0 53 203 60 649.0 0 60 204 48 649.0 0 48 205 45 649.0 0 45 206 53 649.0 0 53 207 56 649.0 0 56 208 201 649.0 0 201 209 66 649.0 0 66 210 57 649.0 0 57 211 53 649.0 0 53 212 60 649.0 0 60 213 60 649.0 0 60 214 93 649.0 0 93 215 94 649.0 0 94 216 107 649.0 0 107 217 155 649.0 0 155 218 52 649.0 0 52 219 43 649.0 0 43 220 59 649.0 0 59 221 48 649.0 0 48 222 42 649.0 0 42 223 52 649.0 0 52 224 67 649.0 0 67 225 58 649.0 0 58 226 64 649.0 0 64 227 49 649.0 0 49 228 60 649.0 0 60 229 49 649.0 0 49 230 50 649.0 0 50 231 60 649.0 0 60 232 53 649.0 0 53 233 64 649.0 0 64 234 58 649.0 0 58 235 50 649.0 0 50 236 58 649.0 0 58 237 39 649.0 0 39 238 93 649.0 0 93 239 67 649.0 0 67 240 87 649.0 0 87 241 52 649.0 0 52 242 56 649.0 0 56 243 57 649.0 0 57 244 53 649.0 0 53 245 66 649.0 0 66 246 57 649.0 0 57 247 50 649.0 0 50 248 66 649.0 0 66 249 84 649.0 0 84 250 55 649.0 0 55 251 66 649.0 0 66 252 54 649.0 0 54 253 56 649.0 0 56 254 62 649.0 0 62 255 78 649.0 0 78 256 55 649.0 0 55 257 103 649.0 0 103 258 83 649.0 0 83 259 80 649.0 0 80 260 103 649.0 0 103 261 67 649.0 0 67 262 95 649.0 0 95 263 86 649.0 0 86 264 72 649.0 0 72 265 67 649.0 0 67 266 95 649.0 0 95 267 95 649.0 0 95 268 91 649.0 0 91 269 93 649.0 0 93 270 114 649.0 0 114 271 114 649.0 0 114 272 268 649.0 0 268 273 1542 649.0 0 1542 274 1403 649.0 0 1403 275 3699 649.0 0 3699 276 112 649.0 0 112 277 94 649.0 0 94 278 114 649.0 0 114 279 127 649.0 0 127 280 143 649.0 0 143 281 83 649.0 0 83 282 204 649.0 0 204 283 519 649.0 0 519 284 357 649.0 0 357 285 700 649.0 0 700 286 584 649.0 0 584 287 454 649.0 0 454 288 299 649.0 0 299 289 123 649.0 0 123 290 133 649.0 0 133 291 164 649.0 0 164 292 304 649.0 0 304 293 3857 649.0 0 3857 294 2911 649.0 0 2911 295 2139 649.0 0 2139 296 247 649.0 0 247 297 778 649.0 0 778 298 2235 649.0 0 2235 299 6077 649.0 0 6077 300 7154 649.0 0 7154 301 3484 649.0 0 3484 Finished in 0.76 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 16,211 Reads with adapters: 2,718 (16.8%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 2,712 (16.7%) Total basepairs processed: 3,575,800 bp Total written (filtered): 731,408 bp (20.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2718 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 27 0.0 3 0 26 0 1 20 2608 0.0 4 2547 59 1 0 1 21 6 0.0 4 3 3 22 1 0.0 4 1 23 6 0.0 4 3 0 0 3 72 1 0.0 4 1 78 1 0.0 4 1 94 1 0.0 4 0 0 0 0 1 103 2 0.0 4 2 106 1 0.0 4 0 0 0 0 1 127 57 0.0 4 0 0 0 0 57 128 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 2,712 Reads with adapters: 329 (12.1%) Reads written (passing filters): 2,712 (100.0%) Total basepairs processed: 731,408 bp Total written (filtered): 718,802 bp (98.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 329 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 5.8% G: 80.2% T: 12.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 0.7 0 1 9 88 0.0 0 4 84 10 163 0.0 1 5 158 11 32 0.0 1 0 32 12 1 0.0 1 0 1 222 1 0.0 1 0 1 223 41 0.0 1 40 1 224 1 0.0 1 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.