This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.91 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 158,777 (6.0%) Reads that were too short: 21,892 (0.8%) Reads written (passing filters): 136,885 (5.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 36,021,411 bp (4.5%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 158777 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4609 649.0 0 4609 7 47458 649.0 0 47458 8 44027 649.0 0 44027 9 59 649.0 0 59 10 23 649.0 0 23 11 19 649.0 0 19 12 25 649.0 0 25 13 19 649.0 0 19 14 26 649.0 0 26 15 35 649.0 0 35 16 20 649.0 0 20 17 25 649.0 0 25 18 23 649.0 0 23 19 18 649.0 0 18 20 21 649.0 0 21 21 16 649.0 0 16 22 15 649.0 0 15 23 22 649.0 0 22 24 21 649.0 0 21 25 22 649.0 0 22 26 24 649.0 0 24 27 17 649.0 0 17 28 34 649.0 0 34 29 20 649.0 0 20 30 48 649.0 0 48 31 137 649.0 0 137 32 63 649.0 0 63 33 72 649.0 0 72 34 81 649.0 0 81 35 32 649.0 0 32 36 51 649.0 0 51 37 76 649.0 0 76 38 38 649.0 0 38 39 57 649.0 0 57 40 53 649.0 0 53 41 59 649.0 0 59 42 32 649.0 0 32 43 87 649.0 0 87 44 77 649.0 0 77 45 70 649.0 0 70 46 84 649.0 0 84 47 128 649.0 0 128 48 152 649.0 0 152 49 261 649.0 0 261 50 5541 649.0 0 5541 51 1553 649.0 0 1553 52 4946 649.0 0 4946 53 53 649.0 0 53 54 53 649.0 0 53 55 13 649.0 0 13 56 20 649.0 0 20 57 26 649.0 0 26 58 35 649.0 0 35 59 31 649.0 0 31 60 56 649.0 0 56 61 44 649.0 0 44 62 29 649.0 0 29 63 57 649.0 0 57 64 97 649.0 0 97 65 45 649.0 0 45 66 58 649.0 0 58 67 48 649.0 0 48 68 76 649.0 0 76 69 42 649.0 0 42 70 25 649.0 0 25 71 73 649.0 0 73 72 63 649.0 0 63 73 116 649.0 0 116 74 685 649.0 0 685 75 442 649.0 0 442 76 2092 649.0 0 2092 77 111 649.0 0 111 78 280 649.0 0 280 79 58 649.0 0 58 80 142 649.0 0 142 81 39 649.0 0 39 82 52 649.0 0 52 83 30 649.0 0 30 84 39 649.0 0 39 85 44 649.0 0 44 86 44 649.0 0 44 87 58 649.0 0 58 88 38 649.0 0 38 89 57 649.0 0 57 90 42 649.0 0 42 91 83 649.0 0 83 92 42 649.0 0 42 93 148 649.0 0 148 94 116 649.0 0 116 95 64 649.0 0 64 96 55 649.0 0 55 97 33 649.0 0 33 98 38 649.0 0 38 99 40 649.0 0 40 100 36 649.0 0 36 101 38 649.0 0 38 102 28 649.0 0 28 103 36 649.0 0 36 104 45 649.0 0 45 105 16 649.0 0 16 106 30 649.0 0 30 107 32 649.0 0 32 108 15 649.0 0 15 109 23 649.0 0 23 110 30 649.0 0 30 111 50 649.0 0 50 112 35 649.0 0 35 113 32 649.0 0 32 114 93 649.0 0 93 115 137 649.0 0 137 116 101 649.0 0 101 117 116 649.0 0 116 118 92 649.0 0 92 119 73 649.0 0 73 120 81 649.0 0 81 121 57 649.0 0 57 122 85 649.0 0 85 123 71 649.0 0 71 124 123 649.0 0 123 125 1050 649.0 0 1050 126 610 649.0 0 610 127 1706 649.0 0 1706 128 81 649.0 0 81 129 83 649.0 0 83 130 244 649.0 0 244 131 142 649.0 0 142 132 253 649.0 0 253 133 46 649.0 0 46 134 58 649.0 0 58 135 246 649.0 0 246 136 145 649.0 0 145 137 225 649.0 0 225 138 42 649.0 0 42 139 33 649.0 0 33 140 48 649.0 0 48 141 38 649.0 0 38 142 48 649.0 0 48 143 59 649.0 0 59 144 61 649.0 0 61 145 60 649.0 0 60 146 57 649.0 0 57 147 40 649.0 0 40 148 50 649.0 0 50 149 40 649.0 0 40 150 56 649.0 0 56 151 74 649.0 0 74 152 45 649.0 0 45 153 74 649.0 0 74 154 97 649.0 0 97 155 57 649.0 0 57 156 51 649.0 0 51 157 71 649.0 0 71 158 84 649.0 0 84 159 74 649.0 0 74 160 87 649.0 0 87 161 98 649.0 0 98 162 115 649.0 0 115 163 187 649.0 0 187 164 1929 649.0 0 1929 165 887 649.0 0 887 166 2621 649.0 0 2621 167 63 649.0 0 63 168 51 649.0 0 51 169 62 649.0 0 62 170 62 649.0 0 62 171 62 649.0 0 62 172 55 649.0 0 55 173 48 649.0 0 48 174 63 649.0 0 63 175 46 649.0 0 46 176 47 649.0 0 47 177 54 649.0 0 54 178 82 649.0 0 82 179 80 649.0 0 80 180 65 649.0 0 65 181 90 649.0 0 90 182 77 649.0 0 77 183 77 649.0 0 77 184 67 649.0 0 67 185 57 649.0 0 57 186 73 649.0 0 73 187 67 649.0 0 67 188 65 649.0 0 65 189 213 649.0 0 213 190 191 649.0 0 191 191 949 649.0 0 949 192 369 649.0 0 369 193 559 649.0 0 559 194 173 649.0 0 173 195 131 649.0 0 131 196 890 649.0 0 890 197 712 649.0 0 712 198 740 649.0 0 740 199 414 649.0 0 414 200 222 649.0 0 222 201 134 649.0 0 134 202 122 649.0 0 122 203 87 649.0 0 87 204 152 649.0 0 152 205 76 649.0 0 76 206 159 649.0 0 159 207 64 649.0 0 64 208 88 649.0 0 88 209 85 649.0 0 85 210 82 649.0 0 82 211 93 649.0 0 93 212 94 649.0 0 94 213 70 649.0 0 70 214 88 649.0 0 88 215 178 649.0 0 178 216 428 649.0 0 428 217 690 649.0 0 690 218 492 649.0 0 492 219 269 649.0 0 269 220 181 649.0 0 181 221 142 649.0 0 142 222 146 649.0 0 146 223 107 649.0 0 107 224 88 649.0 0 88 225 92 649.0 0 92 226 75 649.0 0 75 227 89 649.0 0 89 228 80 649.0 0 80 229 70 649.0 0 70 230 90 649.0 0 90 231 64 649.0 0 64 232 103 649.0 0 103 233 59 649.0 0 59 234 126 649.0 0 126 235 72 649.0 0 72 236 92 649.0 0 92 237 94 649.0 0 94 238 137 649.0 0 137 239 170 649.0 0 170 240 202 649.0 0 202 241 190 649.0 0 190 242 150 649.0 0 150 243 140 649.0 0 140 244 88 649.0 0 88 245 131 649.0 0 131 246 160 649.0 0 160 247 843 649.0 0 843 248 896 649.0 0 896 249 1376 649.0 0 1376 250 1523 649.0 0 1523 251 396 649.0 0 396 252 218 649.0 0 218 253 148 649.0 0 148 254 116 649.0 0 116 255 75 649.0 0 75 256 108 649.0 0 108 257 116 649.0 0 116 258 109 649.0 0 109 259 94 649.0 0 94 260 124 649.0 0 124 261 89 649.0 0 89 262 185 649.0 0 185 263 173 649.0 0 173 264 162 649.0 0 162 265 158 649.0 0 158 266 119 649.0 0 119 267 74 649.0 0 74 268 66 649.0 0 66 269 488 649.0 0 488 270 222 649.0 0 222 271 164 649.0 0 164 272 89 649.0 0 89 273 84 649.0 0 84 274 78 649.0 0 78 275 68 649.0 0 68 276 108 649.0 0 108 277 85 649.0 0 85 278 124 649.0 0 124 279 90 649.0 0 90 280 89 649.0 0 89 281 76 649.0 0 76 282 86 649.0 0 86 283 104 649.0 0 104 284 125 649.0 0 125 285 133 649.0 0 133 286 105 649.0 0 105 287 132 649.0 0 132 288 137 649.0 0 137 289 620 649.0 0 620 290 877 649.0 0 877 291 951 649.0 0 951 292 733 649.0 0 733 293 264 649.0 0 264 294 120 649.0 0 120 295 130 649.0 0 130 296 158 649.0 0 158 297 280 649.0 0 280 298 400 649.0 0 400 299 533 649.0 0 533 300 300 649.0 0 300 301 316 649.0 0 316 Finished in 6.35 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 136,885 Reads with adapters: 50,218 (36.7%) Reads that were too short: 231 (0.2%) Reads written (passing filters): 49,987 (36.5%) Total basepairs processed: 36,021,411 bp Total written (filtered): 13,772,339 bp (38.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50218 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 24 0.0 3 0 0 19 5 16 390 0.0 3 0 330 35 25 17 48670 0.0 3 45919 2256 400 95 18 183 0.0 3 39 70 12 62 19 126 0.0 3 2 0 82 42 20 108 0.0 3 2 0 1 105 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 26 9 0.0 3 0 0 0 9 27 111 0.0 3 0 0 0 111 28 5 0.0 3 0 0 1 4 29 1 0.0 3 0 0 0 1 30 6 0.0 3 0 0 0 6 32 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 76 0.0 3 0 0 1 75 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 9 0.0 3 0 0 0 9 50 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 5 0.0 3 0 0 0 5 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 12 0.0 3 0 0 1 11 64 2 0.0 3 0 0 0 2 65 4 0.0 3 0 0 0 4 66 2 0.0 3 0 0 0 2 67 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 73 2 0.0 3 0 0 1 1 74 1 0.0 3 0 0 0 1 75 54 0.0 3 0 0 2 52 76 1 0.0 3 0 0 0 1 77 5 0.0 3 0 0 0 5 78 5 0.0 3 0 0 0 5 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 15 0.0 3 0 0 1 14 88 2 0.0 3 0 0 0 2 89 4 0.0 3 0 0 0 4 90 9 0.0 3 0 0 0 9 91 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 9 0.0 3 0 0 2 7 96 3 0.0 3 0 0 0 3 97 18 0.0 3 0 0 0 18 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 4 0.0 3 0 0 0 4 115 9 0.0 3 0 0 1 8 116 7 0.0 3 0 0 0 7 117 37 0.0 3 0 0 0 37 118 17 0.0 3 0 0 1 16 119 4 0.0 3 0 0 0 4 120 11 0.0 3 0 0 0 11 121 14 0.0 3 0 0 1 13 122 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 8 0.0 3 0 0 1 7 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 1 3 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 158 10 0.0 3 0 0 0 10 159 1 0.0 3 0 0 0 1 160 4 0.0 3 0 0 0 4 164 4 0.0 3 0 1 0 3 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 4 0.0 3 0 0 0 4 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 177 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 1 180 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 1 2 194 4 0.0 3 0 0 0 4 195 2 0.0 3 0 0 0 2 203 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 209 2 0.0 3 0 0 0 2 214 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 4 0.0 3 0 0 0 4 219 3 0.0 3 0 0 0 3 220 2 0.0 3 0 0 1 1 222 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 248 4 0.0 3 0 0 0 4 249 1 0.0 3 0 0 0 1 250 4 0.0 3 0 0 0 4 264 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 6 0.0 3 0 0 1 5 281 10 0.0 3 0 0 1 9 282 8 0.0 3 0 0 0 8 283 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 1 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.75 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 49,987 Reads with adapters: 433 (0.9%) Reads written (passing filters): 49,987 (100.0%) Total basepairs processed: 13,772,339 bp Total written (filtered): 13,771,012 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 433 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.2% C: 55.4% G: 18.5% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 418 781.0 0 418 4 6 195.3 0 6 5 5 48.8 0 5 6 4 12.2 0 4