This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.39 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 129,795 (4.9%) Reads that were too short: 85,677 (3.2%) Reads written (passing filters): 44,118 (1.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 7,667,138 bp (1.0%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 129795 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 463 649.0 0 463 7 71 649.0 0 71 8 70 649.0 0 70 9 50 649.0 0 50 10 293 649.0 0 293 11 34 649.0 0 34 12 50 649.0 0 50 13 32 649.0 0 32 14 33 649.0 0 33 15 61 649.0 0 61 16 107 649.0 0 107 17 63 649.0 0 63 18 56 649.0 0 56 19 42 649.0 0 42 20 30 649.0 0 30 21 32 649.0 0 32 22 40 649.0 0 40 23 44 649.0 0 44 24 31 649.0 0 31 25 36 649.0 0 36 26 40 649.0 0 40 27 43 649.0 0 43 28 59 649.0 0 59 29 40 649.0 0 40 30 48 649.0 0 48 31 39 649.0 0 39 32 55 649.0 0 55 33 41 649.0 0 41 34 49 649.0 0 49 35 64 649.0 0 64 36 36 649.0 0 36 37 97 649.0 0 97 38 106 649.0 0 106 39 90 649.0 0 90 40 735 649.0 0 735 41 340 649.0 0 340 42 76 649.0 0 76 43 46 649.0 0 46 44 36 649.0 0 36 45 43 649.0 0 43 46 36 649.0 0 36 47 40 649.0 0 40 48 51 649.0 0 51 49 50 649.0 0 50 50 71 649.0 0 71 51 58 649.0 0 58 52 51 649.0 0 51 53 61 649.0 0 61 54 55 649.0 0 55 55 100 649.0 0 100 56 237 649.0 0 237 57 2002 649.0 0 2002 58 495 649.0 0 495 59 444 649.0 0 444 60 38 649.0 0 38 61 37 649.0 0 37 62 32 649.0 0 32 63 52 649.0 0 52 64 35 649.0 0 35 65 53 649.0 0 53 66 50 649.0 0 50 67 50 649.0 0 50 68 62 649.0 0 62 69 62 649.0 0 62 70 41 649.0 0 41 71 41 649.0 0 41 72 45 649.0 0 45 73 56 649.0 0 56 74 68 649.0 0 68 75 107 649.0 0 107 76 84 649.0 0 84 77 48 649.0 0 48 78 88 649.0 0 88 79 93 649.0 0 93 80 107 649.0 0 107 81 68 649.0 0 68 82 109 649.0 0 109 83 100 649.0 0 100 84 92 649.0 0 92 85 56 649.0 0 56 86 62 649.0 0 62 87 59 649.0 0 59 88 164 649.0 0 164 89 120 649.0 0 120 90 124 649.0 0 124 91 117 649.0 0 117 92 81 649.0 0 81 93 92 649.0 0 92 94 70 649.0 0 70 95 62 649.0 0 62 96 88 649.0 0 88 97 71 649.0 0 71 98 76 649.0 0 76 99 85 649.0 0 85 100 125 649.0 0 125 101 115 649.0 0 115 102 80 649.0 0 80 103 93 649.0 0 93 104 165 649.0 0 165 105 205 649.0 0 205 106 151 649.0 0 151 107 280 649.0 0 280 108 134 649.0 0 134 109 192 649.0 0 192 110 97 649.0 0 97 111 56 649.0 0 56 112 63 649.0 0 63 113 84 649.0 0 84 114 75 649.0 0 75 115 117 649.0 0 117 116 106 649.0 0 106 117 122 649.0 0 122 118 99 649.0 0 99 119 116 649.0 0 116 120 170 649.0 0 170 121 356 649.0 0 356 122 1747 649.0 0 1747 123 695 649.0 0 695 124 772 649.0 0 772 125 103 649.0 0 103 126 135 649.0 0 135 127 211 649.0 0 211 128 148 649.0 0 148 129 117 649.0 0 117 130 223 649.0 0 223 131 144 649.0 0 144 132 128 649.0 0 128 133 111 649.0 0 111 134 130 649.0 0 130 135 153 649.0 0 153 136 201 649.0 0 201 137 158 649.0 0 158 138 112 649.0 0 112 139 104 649.0 0 104 140 151 649.0 0 151 141 523 649.0 0 523 142 450 649.0 0 450 143 367 649.0 0 367 144 199 649.0 0 199 145 138 649.0 0 138 146 160 649.0 0 160 147 292 649.0 0 292 148 279 649.0 0 279 149 393 649.0 0 393 150 395 649.0 0 395 151 3125 649.0 0 3125 152 5262 649.0 0 5262 153 4087 649.0 0 4087 154 164 649.0 0 164 155 151 649.0 0 151 156 169 649.0 0 169 157 772 649.0 0 772 158 337 649.0 0 337 159 381 649.0 0 381 160 159 649.0 0 159 161 292 649.0 0 292 162 473 649.0 0 473 163 354 649.0 0 354 164 155 649.0 0 155 165 116 649.0 0 116 166 124 649.0 0 124 167 158 649.0 0 158 168 151 649.0 0 151 169 130 649.0 0 130 170 108 649.0 0 108 171 84 649.0 0 84 172 67 649.0 0 67 173 101 649.0 0 101 174 108 649.0 0 108 175 142 649.0 0 142 176 244 649.0 0 244 177 189 649.0 0 189 178 147 649.0 0 147 179 87 649.0 0 87 180 88 649.0 0 88 181 111 649.0 0 111 182 320 649.0 0 320 183 262 649.0 0 262 184 254 649.0 0 254 185 147 649.0 0 147 186 198 649.0 0 198 187 283 649.0 0 283 188 286 649.0 0 286 189 213 649.0 0 213 190 102 649.0 0 102 191 89 649.0 0 89 192 81 649.0 0 81 193 116 649.0 0 116 194 181 649.0 0 181 195 265 649.0 0 265 196 328 649.0 0 328 197 198 649.0 0 198 198 163 649.0 0 163 199 104 649.0 0 104 200 135 649.0 0 135 201 200 649.0 0 200 202 181 649.0 0 181 203 166 649.0 0 166 204 160 649.0 0 160 205 206 649.0 0 206 206 183 649.0 0 183 207 286 649.0 0 286 208 559 649.0 0 559 209 1596 649.0 0 1596 210 1339 649.0 0 1339 211 1514 649.0 0 1514 212 2916 649.0 0 2916 213 26388 649.0 0 26388 214 8736 649.0 0 8736 215 11337 649.0 0 11337 216 562 649.0 0 562 217 216 649.0 0 216 218 196 649.0 0 196 219 240 649.0 0 240 220 653 649.0 0 653 221 2805 649.0 0 2805 222 3248 649.0 0 3248 223 2441 649.0 0 2441 224 293 649.0 0 293 225 132 649.0 0 132 226 160 649.0 0 160 227 139 649.0 0 139 228 120 649.0 0 120 229 110 649.0 0 110 230 87 649.0 0 87 231 209 649.0 0 209 232 210 649.0 0 210 233 211 649.0 0 211 234 193 649.0 0 193 235 252 649.0 0 252 236 170 649.0 0 170 237 163 649.0 0 163 238 180 649.0 0 180 239 185 649.0 0 185 240 163 649.0 0 163 241 136 649.0 0 136 242 213 649.0 0 213 243 263 649.0 0 263 244 393 649.0 0 393 245 332 649.0 0 332 246 203 649.0 0 203 247 102 649.0 0 102 248 101 649.0 0 101 249 114 649.0 0 114 250 122 649.0 0 122 251 119 649.0 0 119 252 112 649.0 0 112 253 141 649.0 0 141 254 158 649.0 0 158 255 187 649.0 0 187 256 325 649.0 0 325 257 672 649.0 0 672 258 560 649.0 0 560 259 531 649.0 0 531 260 273 649.0 0 273 261 223 649.0 0 223 262 251 649.0 0 251 263 608 649.0 0 608 264 706 649.0 0 706 265 625 649.0 0 625 266 215 649.0 0 215 267 128 649.0 0 128 268 152 649.0 0 152 269 246 649.0 0 246 270 397 649.0 0 397 271 323 649.0 0 323 272 277 649.0 0 277 273 179 649.0 0 179 274 445 649.0 0 445 275 446 649.0 0 446 276 298 649.0 0 298 277 314 649.0 0 314 278 257 649.0 0 257 279 168 649.0 0 168 280 143 649.0 0 143 281 268 649.0 0 268 282 275 649.0 0 275 283 408 649.0 0 408 284 366 649.0 0 366 285 357 649.0 0 357 286 283 649.0 0 283 287 141 649.0 0 141 288 176 649.0 0 176 289 301 649.0 0 301 290 344 649.0 0 344 291 362 649.0 0 362 292 185 649.0 0 185 293 110 649.0 0 110 294 152 649.0 0 152 295 203 649.0 0 203 296 319 649.0 0 319 297 267 649.0 0 267 298 300 649.0 0 300 299 204 649.0 0 204 300 142 649.0 0 142 301 151 649.0 0 151 Finished in 1.56 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 44,118 Reads with adapters: 1,202 (2.7%) Reads that were too short: 129 (0.3%) Reads written (passing filters): 1,073 (2.4%) Total basepairs processed: 7,667,138 bp Total written (filtered): 116,996 bp (1.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1202 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 4 0.0 3 0 0 4 17 22 0.0 3 17 5 18 2 0.0 3 0 0 0 2 19 7 0.0 3 6 1 20 1 0.0 3 1 30 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 4 0.0 3 0 0 0 4 44 1 0.0 3 0 0 1 45 2 0.0 3 0 0 0 2 47 3 0.0 3 0 0 0 3 50 4 0.0 3 0 0 0 4 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 1 1 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 80 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 1 88 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 16 0.0 3 0 0 0 16 133 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 1 0.0 3 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 160 1013 0.0 3 0 0 4 1009 161 1 0.0 3 0 0 1 162 4 0.0 3 0 0 0 4 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 1 166 3 0.0 3 0 0 1 2 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 1 3 187 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 1 203 1 0.0 3 0 0 0 1 213 1 0.0 3 1 214 1 0.0 3 0 0 1 215 1 0.0 3 0 0 1 216 1 0.0 3 1 232 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (19 us/read; 3.22 M reads/minute). === Summary === Total reads processed: 1,073 Reads with adapters: 13 (1.2%) Reads written (passing filters): 1,073 (100.0%) Total basepairs processed: 116,996 bp Total written (filtered): 116,955 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.2% C: 23.1% G: 7.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 16.8 0 12 5 1 1.0 0 1