This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.78 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 118,931 (4.5%) Reads that were too short: 78,377 (2.9%) Reads written (passing filters): 40,554 (1.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 7,116,949 bp (0.9%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 118931 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 526 649.0 0 526 7 77 649.0 0 77 8 32 649.0 0 32 9 60 649.0 0 60 10 159 649.0 0 159 11 19 649.0 0 19 12 14 649.0 0 14 13 18 649.0 0 18 14 36 649.0 0 36 15 35 649.0 0 35 16 31 649.0 0 31 17 33 649.0 0 33 18 51 649.0 0 51 19 37 649.0 0 37 20 28 649.0 0 28 21 18 649.0 0 18 22 30 649.0 0 30 23 39 649.0 0 39 24 24 649.0 0 24 25 22 649.0 0 22 26 39 649.0 0 39 27 656 649.0 0 656 28 68 649.0 0 68 29 25 649.0 0 25 30 29 649.0 0 29 31 31 649.0 0 31 32 37 649.0 0 37 33 33 649.0 0 33 34 36 649.0 0 36 35 44 649.0 0 44 36 40 649.0 0 40 37 81 649.0 0 81 38 71 649.0 0 71 39 62 649.0 0 62 40 736 649.0 0 736 41 383 649.0 0 383 42 75 649.0 0 75 43 31 649.0 0 31 44 33 649.0 0 33 45 35 649.0 0 35 46 34 649.0 0 34 47 29 649.0 0 29 48 41 649.0 0 41 49 22 649.0 0 22 50 57 649.0 0 57 51 49 649.0 0 49 52 47 649.0 0 47 53 49 649.0 0 49 54 57 649.0 0 57 55 95 649.0 0 95 56 201 649.0 0 201 57 2178 649.0 0 2178 58 476 649.0 0 476 59 380 649.0 0 380 60 30 649.0 0 30 61 28 649.0 0 28 62 23 649.0 0 23 63 36 649.0 0 36 64 31 649.0 0 31 65 33 649.0 0 33 66 29 649.0 0 29 67 29 649.0 0 29 68 41 649.0 0 41 69 48 649.0 0 48 70 29 649.0 0 29 71 35 649.0 0 35 72 24 649.0 0 24 73 31 649.0 0 31 74 44 649.0 0 44 75 38 649.0 0 38 76 32 649.0 0 32 77 40 649.0 0 40 78 74 649.0 0 74 79 103 649.0 0 103 80 112 649.0 0 112 81 85 649.0 0 85 82 125 649.0 0 125 83 92 649.0 0 92 84 124 649.0 0 124 85 66 649.0 0 66 86 75 649.0 0 75 87 70 649.0 0 70 88 370 649.0 0 370 89 209 649.0 0 209 90 279 649.0 0 279 91 128 649.0 0 128 92 205 649.0 0 205 93 98 649.0 0 98 94 47 649.0 0 47 95 69 649.0 0 69 96 58 649.0 0 58 97 56 649.0 0 56 98 114 649.0 0 114 99 79 649.0 0 79 100 63 649.0 0 63 101 100 649.0 0 100 102 100 649.0 0 100 103 114 649.0 0 114 104 145 649.0 0 145 105 179 649.0 0 179 106 185 649.0 0 185 107 161 649.0 0 161 108 115 649.0 0 115 109 163 649.0 0 163 110 93 649.0 0 93 111 63 649.0 0 63 112 54 649.0 0 54 113 65 649.0 0 65 114 70 649.0 0 70 115 119 649.0 0 119 116 76 649.0 0 76 117 109 649.0 0 109 118 63 649.0 0 63 119 62 649.0 0 62 120 92 649.0 0 92 121 153 649.0 0 153 122 728 649.0 0 728 123 494 649.0 0 494 124 453 649.0 0 453 125 73 649.0 0 73 126 103 649.0 0 103 127 172 649.0 0 172 128 130 649.0 0 130 129 109 649.0 0 109 130 173 649.0 0 173 131 102 649.0 0 102 132 107 649.0 0 107 133 105 649.0 0 105 134 148 649.0 0 148 135 154 649.0 0 154 136 124 649.0 0 124 137 118 649.0 0 118 138 105 649.0 0 105 139 102 649.0 0 102 140 106 649.0 0 106 141 399 649.0 0 399 142 340 649.0 0 340 143 301 649.0 0 301 144 140 649.0 0 140 145 106 649.0 0 106 146 154 649.0 0 154 147 225 649.0 0 225 148 245 649.0 0 245 149 312 649.0 0 312 150 389 649.0 0 389 151 3247 649.0 0 3247 152 5731 649.0 0 5731 153 4431 649.0 0 4431 154 119 649.0 0 119 155 145 649.0 0 145 156 173 649.0 0 173 157 890 649.0 0 890 158 299 649.0 0 299 159 352 649.0 0 352 160 108 649.0 0 108 161 220 649.0 0 220 162 285 649.0 0 285 163 241 649.0 0 241 164 114 649.0 0 114 165 90 649.0 0 90 166 113 649.0 0 113 167 124 649.0 0 124 168 100 649.0 0 100 169 106 649.0 0 106 170 93 649.0 0 93 171 82 649.0 0 82 172 60 649.0 0 60 173 80 649.0 0 80 174 84 649.0 0 84 175 120 649.0 0 120 176 163 649.0 0 163 177 128 649.0 0 128 178 110 649.0 0 110 179 89 649.0 0 89 180 78 649.0 0 78 181 101 649.0 0 101 182 262 649.0 0 262 183 203 649.0 0 203 184 194 649.0 0 194 185 108 649.0 0 108 186 174 649.0 0 174 187 214 649.0 0 214 188 192 649.0 0 192 189 142 649.0 0 142 190 83 649.0 0 83 191 84 649.0 0 84 192 79 649.0 0 79 193 94 649.0 0 94 194 144 649.0 0 144 195 247 649.0 0 247 196 244 649.0 0 244 197 134 649.0 0 134 198 121 649.0 0 121 199 97 649.0 0 97 200 131 649.0 0 131 201 182 649.0 0 182 202 149 649.0 0 149 203 125 649.0 0 125 204 122 649.0 0 122 205 142 649.0 0 142 206 187 649.0 0 187 207 224 649.0 0 224 208 371 649.0 0 371 209 906 649.0 0 906 210 820 649.0 0 820 211 1351 649.0 0 1351 212 2739 649.0 0 2739 213 28755 649.0 0 28755 214 10055 649.0 0 10055 215 13101 649.0 0 13101 216 581 649.0 0 581 217 180 649.0 0 180 218 114 649.0 0 114 219 146 649.0 0 146 220 351 649.0 0 351 221 1487 649.0 0 1487 222 1469 649.0 0 1469 223 1176 649.0 0 1176 224 193 649.0 0 193 225 129 649.0 0 129 226 106 649.0 0 106 227 109 649.0 0 109 228 105 649.0 0 105 229 91 649.0 0 91 230 80 649.0 0 80 231 143 649.0 0 143 232 143 649.0 0 143 233 151 649.0 0 151 234 149 649.0 0 149 235 235 649.0 0 235 236 148 649.0 0 148 237 110 649.0 0 110 238 158 649.0 0 158 239 145 649.0 0 145 240 119 649.0 0 119 241 112 649.0 0 112 242 169 649.0 0 169 243 166 649.0 0 166 244 214 649.0 0 214 245 159 649.0 0 159 246 116 649.0 0 116 247 85 649.0 0 85 248 99 649.0 0 99 249 94 649.0 0 94 250 104 649.0 0 104 251 95 649.0 0 95 252 101 649.0 0 101 253 119 649.0 0 119 254 114 649.0 0 114 255 167 649.0 0 167 256 201 649.0 0 201 257 347 649.0 0 347 258 336 649.0 0 336 259 343 649.0 0 343 260 233 649.0 0 233 261 149 649.0 0 149 262 169 649.0 0 169 263 343 649.0 0 343 264 346 649.0 0 346 265 325 649.0 0 325 266 179 649.0 0 179 267 76 649.0 0 76 268 97 649.0 0 97 269 189 649.0 0 189 270 272 649.0 0 272 271 185 649.0 0 185 272 240 649.0 0 240 273 175 649.0 0 175 274 456 649.0 0 456 275 346 649.0 0 346 276 227 649.0 0 227 277 215 649.0 0 215 278 171 649.0 0 171 279 151 649.0 0 151 280 117 649.0 0 117 281 187 649.0 0 187 282 196 649.0 0 196 283 282 649.0 0 282 284 229 649.0 0 229 285 198 649.0 0 198 286 186 649.0 0 186 287 138 649.0 0 138 288 132 649.0 0 132 289 184 649.0 0 184 290 251 649.0 0 251 291 204 649.0 0 204 292 134 649.0 0 134 293 100 649.0 0 100 294 154 649.0 0 154 295 177 649.0 0 177 296 231 649.0 0 231 297 211 649.0 0 211 298 214 649.0 0 214 299 126 649.0 0 126 300 93 649.0 0 93 301 83 649.0 0 83 Finished in 1.55 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 40,554 Reads with adapters: 655 (1.6%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 643 (1.6%) Total basepairs processed: 7,116,949 bp Total written (filtered): 160,943 bp (2.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 655 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 1 0.0 3 0 1 20 39 0.0 4 38 1 21 2 0.0 4 0 0 1 0 1 22 11 0.0 4 1 10 23 492 0.0 4 485 4 3 26 1 0.0 4 0 0 0 1 27 93 0.0 4 0 0 0 1 92 43 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 126 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 1 218 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 643 Reads with adapters: 105 (16.3%) Reads written (passing filters): 643 (100.0%) Total basepairs processed: 160,943 bp Total written (filtered): 159,696 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 105 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 3.8% G: 91.4% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 10.0 0 2 5 1 0.6 0 1 9 65 0.0 0 0 65 10 30 0.0 1 2 28 11 5 0.0 1 0 5 125 1 0.0 1 0 1 171 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.