This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.84 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 370,858 (14.0%) Reads that were too short: 20,346 (0.8%) Reads written (passing filters): 350,512 (13.2%) Total basepairs processed: 800,111,879 bp Total written (filtered): 72,605,760 bp (9.1%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 370858 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 66 649.0 0 66 7 67 649.0 0 67 8 60 649.0 0 60 9 100 649.0 0 100 10 63 649.0 0 63 11 65 649.0 0 65 12 80 649.0 0 80 13 73 649.0 0 73 14 58 649.0 0 58 15 66 649.0 0 66 16 46 649.0 0 46 17 72 649.0 0 72 18 97 649.0 0 97 19 86 649.0 0 86 20 88 649.0 0 88 21 77 649.0 0 77 22 87 649.0 0 87 23 107 649.0 0 107 24 118 649.0 0 118 25 88 649.0 0 88 26 71 649.0 0 71 27 73 649.0 0 73 28 77 649.0 0 77 29 82 649.0 0 82 30 85 649.0 0 85 31 100 649.0 0 100 32 109 649.0 0 109 33 99 649.0 0 99 34 79 649.0 0 79 35 121 649.0 0 121 36 161 649.0 0 161 37 129 649.0 0 129 38 216 649.0 0 216 39 157 649.0 0 157 40 159 649.0 0 159 41 115 649.0 0 115 42 154 649.0 0 154 43 131 649.0 0 131 44 166 649.0 0 166 45 199 649.0 0 199 46 327 649.0 0 327 47 499 649.0 0 499 48 725 649.0 0 725 49 1427 649.0 0 1427 50 384 649.0 0 384 51 494 649.0 0 494 52 842 649.0 0 842 53 651 649.0 0 651 54 524 649.0 0 524 55 732 649.0 0 732 56 763 649.0 0 763 57 1055 649.0 0 1055 58 1013 649.0 0 1013 59 2023 649.0 0 2023 60 2224 649.0 0 2224 61 4752 649.0 0 4752 62 88574 649.0 0 88574 63 53186 649.0 0 53186 64 55430 649.0 0 55430 65 745 649.0 0 745 66 237 649.0 0 237 67 70 649.0 0 70 68 108 649.0 0 108 69 92 649.0 0 92 70 168 649.0 0 168 71 433 649.0 0 433 72 341 649.0 0 341 73 365 649.0 0 365 74 299 649.0 0 299 75 499 649.0 0 499 76 5302 649.0 0 5302 77 4920 649.0 0 4920 78 2316 649.0 0 2316 79 1341 649.0 0 1341 80 129 649.0 0 129 81 167 649.0 0 167 82 87 649.0 0 87 83 98 649.0 0 98 84 95 649.0 0 95 85 113 649.0 0 113 86 79 649.0 0 79 87 97 649.0 0 97 88 97 649.0 0 97 89 89 649.0 0 89 90 78 649.0 0 78 91 80 649.0 0 80 92 89 649.0 0 89 93 148 649.0 0 148 94 131 649.0 0 131 95 111 649.0 0 111 96 71 649.0 0 71 97 77 649.0 0 77 98 150 649.0 0 150 99 745 649.0 0 745 100 414 649.0 0 414 101 74 649.0 0 74 102 110 649.0 0 110 103 156 649.0 0 156 104 128 649.0 0 128 105 94 649.0 0 94 106 156 649.0 0 156 107 558 649.0 0 558 108 923 649.0 0 923 109 813 649.0 0 813 110 159 649.0 0 159 111 121 649.0 0 121 112 116 649.0 0 116 113 55 649.0 0 55 114 69 649.0 0 69 115 94 649.0 0 94 116 83 649.0 0 83 117 101 649.0 0 101 118 88 649.0 0 88 119 82 649.0 0 82 120 97 649.0 0 97 121 96 649.0 0 96 122 62 649.0 0 62 123 99 649.0 0 99 124 109 649.0 0 109 125 114 649.0 0 114 126 203 649.0 0 203 127 236 649.0 0 236 128 179 649.0 0 179 129 247 649.0 0 247 130 130 649.0 0 130 131 116 649.0 0 116 132 93 649.0 0 93 133 100 649.0 0 100 134 115 649.0 0 115 135 111 649.0 0 111 136 120 649.0 0 120 137 207 649.0 0 207 138 164 649.0 0 164 139 168 649.0 0 168 140 211 649.0 0 211 141 200 649.0 0 200 142 373 649.0 0 373 143 611 649.0 0 611 144 5280 649.0 0 5280 145 6013 649.0 0 6013 146 4977 649.0 0 4977 147 274 649.0 0 274 148 193 649.0 0 193 149 228 649.0 0 228 150 163 649.0 0 163 151 193 649.0 0 193 152 249 649.0 0 249 153 166 649.0 0 166 154 267 649.0 0 267 155 264 649.0 0 264 156 1448 649.0 0 1448 157 690 649.0 0 690 158 891 649.0 0 891 159 334 649.0 0 334 160 622 649.0 0 622 161 534 649.0 0 534 162 1039 649.0 0 1039 163 1656 649.0 0 1656 164 19973 649.0 0 19973 165 25723 649.0 0 25723 166 13274 649.0 0 13274 167 612 649.0 0 612 168 180 649.0 0 180 169 666 649.0 0 666 170 1068 649.0 0 1068 171 1275 649.0 0 1275 172 1366 649.0 0 1366 173 646 649.0 0 646 174 549 649.0 0 549 175 1832 649.0 0 1832 176 120 649.0 0 120 177 136 649.0 0 136 178 147 649.0 0 147 179 353 649.0 0 353 180 217 649.0 0 217 181 269 649.0 0 269 182 204 649.0 0 204 183 130 649.0 0 130 184 517 649.0 0 517 185 365 649.0 0 365 186 501 649.0 0 501 187 154 649.0 0 154 188 205 649.0 0 205 189 1363 649.0 0 1363 190 1732 649.0 0 1732 191 1421 649.0 0 1421 192 879 649.0 0 879 193 71 649.0 0 71 194 75 649.0 0 75 195 74 649.0 0 74 196 95 649.0 0 95 197 131 649.0 0 131 198 191 649.0 0 191 199 150 649.0 0 150 200 103 649.0 0 103 201 70 649.0 0 70 202 115 649.0 0 115 203 112 649.0 0 112 204 108 649.0 0 108 205 97 649.0 0 97 206 99 649.0 0 99 207 101 649.0 0 101 208 127 649.0 0 127 209 122 649.0 0 122 210 203 649.0 0 203 211 105 649.0 0 105 212 144 649.0 0 144 213 104 649.0 0 104 214 121 649.0 0 121 215 185 649.0 0 185 216 236 649.0 0 236 217 302 649.0 0 302 218 254 649.0 0 254 219 120 649.0 0 120 220 71 649.0 0 71 221 73 649.0 0 73 222 109 649.0 0 109 223 92 649.0 0 92 224 148 649.0 0 148 225 186 649.0 0 186 226 506 649.0 0 506 227 558 649.0 0 558 228 368 649.0 0 368 229 558 649.0 0 558 230 276 649.0 0 276 231 225 649.0 0 225 232 104 649.0 0 104 233 87 649.0 0 87 234 105 649.0 0 105 235 90 649.0 0 90 236 137 649.0 0 137 237 119 649.0 0 119 238 145 649.0 0 145 239 132 649.0 0 132 240 133 649.0 0 133 241 95 649.0 0 95 242 75 649.0 0 75 243 154 649.0 0 154 244 141 649.0 0 141 245 203 649.0 0 203 246 180 649.0 0 180 247 153 649.0 0 153 248 147 649.0 0 147 249 98 649.0 0 98 250 105 649.0 0 105 251 71 649.0 0 71 252 128 649.0 0 128 253 108 649.0 0 108 254 95 649.0 0 95 255 87 649.0 0 87 256 87 649.0 0 87 257 95 649.0 0 95 258 124 649.0 0 124 259 235 649.0 0 235 260 366 649.0 0 366 261 385 649.0 0 385 262 358 649.0 0 358 263 212 649.0 0 212 264 155 649.0 0 155 265 257 649.0 0 257 266 282 649.0 0 282 267 334 649.0 0 334 268 210 649.0 0 210 269 132 649.0 0 132 270 152 649.0 0 152 271 500 649.0 0 500 272 295 649.0 0 295 273 258 649.0 0 258 274 118 649.0 0 118 275 121 649.0 0 121 276 126 649.0 0 126 277 308 649.0 0 308 278 170 649.0 0 170 279 237 649.0 0 237 280 141 649.0 0 141 281 152 649.0 0 152 282 137 649.0 0 137 283 117 649.0 0 117 284 161 649.0 0 161 285 215 649.0 0 215 286 438 649.0 0 438 287 987 649.0 0 987 288 840 649.0 0 840 289 578 649.0 0 578 290 212 649.0 0 212 291 121 649.0 0 121 292 305 649.0 0 305 293 210 649.0 0 210 294 244 649.0 0 244 295 142 649.0 0 142 296 154 649.0 0 154 297 232 649.0 0 232 298 198 649.0 0 198 299 223 649.0 0 223 300 114 649.0 0 114 301 191 649.0 0 191 Finished in 12.34 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 350,512 Reads with adapters: 1,953 (0.6%) Reads that were too short: 644 (0.2%) Reads written (passing filters): 1,309 (0.4%) Total basepairs processed: 72,605,760 bp Total written (filtered): 151,532 bp (0.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1953 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 3 0 1 1 18 2 0.0 3 1 0 0 1 19 2 0.0 3 0 0 1 1 20 1 0.0 3 0 0 0 1 21 17 0.0 3 0 0 2 15 22 2 0.0 3 0 0 0 2 25 4 0.0 3 0 0 1 3 26 4 0.0 3 0 0 0 4 27 2 0.0 3 0 0 0 2 28 91 0.0 3 0 0 5 86 29 28 0.0 3 0 0 0 28 31 3 0.0 3 0 0 0 3 32 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 37 5 0.0 3 0 0 0 5 38 13 0.0 3 0 0 0 13 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 1 1 51 4 0.0 3 0 0 0 4 53 6 0.0 3 0 0 0 6 54 3 0.0 3 0 0 0 3 55 5 0.0 3 0 0 0 5 56 7 0.0 3 0 0 5 2 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 60 4 0.0 3 0 0 0 4 61 8 0.0 3 0 0 1 7 62 10 0.0 3 0 0 0 10 64 9 0.0 3 0 0 0 9 65 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 1 2 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 1 81 5 0.0 3 0 0 0 5 83 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 90 2 0.0 3 0 0 0 2 91 3 0.0 3 0 0 0 3 94 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 1 103 3 0.0 3 0 0 0 3 104 20 0.0 3 0 0 0 20 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 107 8 0.0 3 0 0 0 8 108 3 0.0 3 0 0 0 3 109 41 0.0 3 0 0 0 41 110 1 0.0 3 0 0 0 1 111 4 0.0 3 0 0 0 4 112 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 114 8 0.0 3 0 0 0 8 115 4 0.0 3 0 0 0 4 116 2 0.0 3 0 0 0 2 117 14 0.0 3 0 0 0 14 118 5 0.0 3 0 0 0 5 119 3 0.0 3 0 0 0 3 120 2 0.0 3 0 0 0 2 121 6 0.0 3 0 0 0 6 122 8 0.0 3 0 0 0 8 123 19 0.0 3 0 0 1 18 124 31 0.0 3 0 0 1 30 125 8 0.0 3 0 0 0 8 126 2 0.0 3 0 0 0 2 127 12 0.0 3 0 0 0 12 128 36 0.0 3 0 0 1 35 129 656 0.0 3 0 0 5 651 130 111 0.0 3 0 0 2 109 131 10 0.0 3 0 0 0 10 132 88 0.0 3 0 0 2 86 133 30 0.0 3 1 0 1 28 134 3 0.0 3 2 0 1 135 3 0.0 3 0 0 1 2 136 1 0.0 3 1 138 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 6 0.0 3 0 0 1 5 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 147 4 0.0 3 0 0 0 4 148 7 0.0 3 0 0 0 7 149 2 0.0 3 0 0 1 1 151 1 0.0 3 0 0 0 1 152 5 0.0 3 0 0 1 4 153 7 0.0 3 0 0 1 6 154 8 0.0 3 0 0 1 7 155 35 0.0 3 0 0 1 34 156 8 0.0 3 0 0 0 8 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 164 11 0.0 3 0 0 0 11 165 1 0.0 3 0 0 0 1 167 5 0.0 3 0 0 0 5 168 2 0.0 3 0 1 0 1 169 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 173 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 1 180 37 0.0 3 0 0 1 36 181 13 0.0 3 0 0 0 13 182 5 0.0 3 0 0 0 5 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 19 0.0 3 0 0 0 19 190 8 0.0 3 0 0 0 8 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 1 201 7 0.0 3 0 0 1 6 202 4 0.0 3 0 0 0 4 203 4 0.0 3 0 0 0 4 204 3 0.0 3 0 0 0 3 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 210 3 0.0 3 0 0 0 3 211 5 0.0 3 0 0 0 5 212 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 215 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 219 4 0.0 3 0 0 0 4 220 2 0.0 3 0 0 0 2 221 4 0.0 3 0 0 0 4 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 1 229 5 0.0 3 0 0 0 5 230 50 0.0 3 0 0 3 47 231 118 0.0 3 0 0 8 110 232 10 0.0 3 0 0 0 10 233 4 0.0 3 0 0 0 4 234 4 0.0 3 0 0 0 4 235 1 0.0 3 0 0 0 1 236 10 0.0 3 0 0 1 9 237 3 0.0 3 0 0 0 3 239 5 0.0 3 0 0 0 5 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 2 0.0 3 0 0 0 2 261 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (23 us/read; 2.62 M reads/minute). === Summary === Total reads processed: 1,309 Reads with adapters: 13 (1.0%) Reads written (passing filters): 1,309 (100.0%) Total basepairs processed: 151,532 bp Total written (filtered): 151,490 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.1% C: 23.1% G: 46.2% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10 20.5 0 10 4 3 5.1 0 3