This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.25 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 345,292 (13.0%) Reads that were too short: 12,014 (0.5%) Reads written (passing filters): 333,278 (12.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 69,464,932 bp (8.7%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 345292 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 60 649.0 0 60 7 50 649.0 0 50 8 48 649.0 0 48 9 78 649.0 0 78 10 42 649.0 0 42 11 51 649.0 0 51 12 48 649.0 0 48 13 45 649.0 0 45 14 52 649.0 0 52 15 47 649.0 0 47 16 35 649.0 0 35 17 48 649.0 0 48 18 82 649.0 0 82 19 75 649.0 0 75 20 92 649.0 0 92 21 56 649.0 0 56 22 92 649.0 0 92 23 77 649.0 0 77 24 70 649.0 0 70 25 49 649.0 0 49 26 52 649.0 0 52 27 60 649.0 0 60 28 42 649.0 0 42 29 515 649.0 0 515 30 125 649.0 0 125 31 61 649.0 0 61 32 68 649.0 0 68 33 68 649.0 0 68 34 67 649.0 0 67 35 68 649.0 0 68 36 113 649.0 0 113 37 110 649.0 0 110 38 189 649.0 0 189 39 128 649.0 0 128 40 108 649.0 0 108 41 68 649.0 0 68 42 114 649.0 0 114 43 74 649.0 0 74 44 134 649.0 0 134 45 172 649.0 0 172 46 284 649.0 0 284 47 478 649.0 0 478 48 718 649.0 0 718 49 1306 649.0 0 1306 50 353 649.0 0 353 51 434 649.0 0 434 52 754 649.0 0 754 53 404 649.0 0 404 54 406 649.0 0 406 55 695 649.0 0 695 56 755 649.0 0 755 57 872 649.0 0 872 58 801 649.0 0 801 59 1690 649.0 0 1690 60 1700 649.0 0 1700 61 3845 649.0 0 3845 62 84441 649.0 0 84441 63 52063 649.0 0 52063 64 55724 649.0 0 55724 65 666 649.0 0 666 66 212 649.0 0 212 67 79 649.0 0 79 68 95 649.0 0 95 69 87 649.0 0 87 70 113 649.0 0 113 71 316 649.0 0 316 72 253 649.0 0 253 73 315 649.0 0 315 74 286 649.0 0 286 75 442 649.0 0 442 76 6212 649.0 0 6212 77 6045 649.0 0 6045 78 2654 649.0 0 2654 79 1318 649.0 0 1318 80 105 649.0 0 105 81 130 649.0 0 130 82 59 649.0 0 59 83 51 649.0 0 51 84 53 649.0 0 53 85 72 649.0 0 72 86 64 649.0 0 64 87 57 649.0 0 57 88 59 649.0 0 59 89 46 649.0 0 46 90 63 649.0 0 63 91 81 649.0 0 81 92 63 649.0 0 63 93 110 649.0 0 110 94 81 649.0 0 81 95 81 649.0 0 81 96 65 649.0 0 65 97 54 649.0 0 54 98 116 649.0 0 116 99 712 649.0 0 712 100 362 649.0 0 362 101 57 649.0 0 57 102 67 649.0 0 67 103 70 649.0 0 70 104 105 649.0 0 105 105 66 649.0 0 66 106 79 649.0 0 79 107 234 649.0 0 234 108 243 649.0 0 243 109 249 649.0 0 249 110 93 649.0 0 93 111 85 649.0 0 85 112 91 649.0 0 91 113 64 649.0 0 64 114 43 649.0 0 43 115 96 649.0 0 96 116 70 649.0 0 70 117 72 649.0 0 72 118 78 649.0 0 78 119 80 649.0 0 80 120 83 649.0 0 83 121 67 649.0 0 67 122 48 649.0 0 48 123 65 649.0 0 65 124 105 649.0 0 105 125 84 649.0 0 84 126 149 649.0 0 149 127 158 649.0 0 158 128 150 649.0 0 150 129 224 649.0 0 224 130 122 649.0 0 122 131 85 649.0 0 85 132 108 649.0 0 108 133 75 649.0 0 75 134 95 649.0 0 95 135 78 649.0 0 78 136 71 649.0 0 71 137 186 649.0 0 186 138 145 649.0 0 145 139 122 649.0 0 122 140 143 649.0 0 143 141 158 649.0 0 158 142 361 649.0 0 361 143 533 649.0 0 533 144 5084 649.0 0 5084 145 6101 649.0 0 6101 146 5110 649.0 0 5110 147 228 649.0 0 228 148 160 649.0 0 160 149 217 649.0 0 217 150 122 649.0 0 122 151 174 649.0 0 174 152 261 649.0 0 261 153 156 649.0 0 156 154 227 649.0 0 227 155 194 649.0 0 194 156 1148 649.0 0 1148 157 431 649.0 0 431 158 638 649.0 0 638 159 316 649.0 0 316 160 557 649.0 0 557 161 443 649.0 0 443 162 801 649.0 0 801 163 1367 649.0 0 1367 164 20154 649.0 0 20154 165 27431 649.0 0 27431 166 13843 649.0 0 13843 167 611 649.0 0 611 168 104 649.0 0 104 169 368 649.0 0 368 170 476 649.0 0 476 171 606 649.0 0 606 172 582 649.0 0 582 173 301 649.0 0 301 174 272 649.0 0 272 175 2020 649.0 0 2020 176 80 649.0 0 80 177 70 649.0 0 70 178 104 649.0 0 104 179 254 649.0 0 254 180 195 649.0 0 195 181 212 649.0 0 212 182 178 649.0 0 178 183 107 649.0 0 107 184 256 649.0 0 256 185 202 649.0 0 202 186 168 649.0 0 168 187 63 649.0 0 63 188 101 649.0 0 101 189 468 649.0 0 468 190 598 649.0 0 598 191 547 649.0 0 547 192 432 649.0 0 432 193 53 649.0 0 53 194 43 649.0 0 43 195 35 649.0 0 35 196 46 649.0 0 46 197 64 649.0 0 64 198 109 649.0 0 109 199 75 649.0 0 75 200 70 649.0 0 70 201 55 649.0 0 55 202 74 649.0 0 74 203 79 649.0 0 79 204 72 649.0 0 72 205 72 649.0 0 72 206 68 649.0 0 68 207 53 649.0 0 53 208 82 649.0 0 82 209 107 649.0 0 107 210 148 649.0 0 148 211 94 649.0 0 94 212 68 649.0 0 68 213 76 649.0 0 76 214 74 649.0 0 74 215 111 649.0 0 111 216 134 649.0 0 134 217 158 649.0 0 158 218 141 649.0 0 141 219 75 649.0 0 75 220 64 649.0 0 64 221 53 649.0 0 53 222 52 649.0 0 52 223 58 649.0 0 58 224 93 649.0 0 93 225 129 649.0 0 129 226 289 649.0 0 289 227 305 649.0 0 305 228 198 649.0 0 198 229 548 649.0 0 548 230 289 649.0 0 289 231 224 649.0 0 224 232 77 649.0 0 77 233 50 649.0 0 50 234 53 649.0 0 53 235 66 649.0 0 66 236 67 649.0 0 67 237 74 649.0 0 74 238 74 649.0 0 74 239 77 649.0 0 77 240 64 649.0 0 64 241 68 649.0 0 68 242 42 649.0 0 42 243 81 649.0 0 81 244 65 649.0 0 65 245 86 649.0 0 86 246 79 649.0 0 79 247 90 649.0 0 90 248 139 649.0 0 139 249 70 649.0 0 70 250 59 649.0 0 59 251 56 649.0 0 56 252 77 649.0 0 77 253 60 649.0 0 60 254 72 649.0 0 72 255 73 649.0 0 73 256 64 649.0 0 64 257 64 649.0 0 64 258 69 649.0 0 69 259 99 649.0 0 99 260 158 649.0 0 158 261 142 649.0 0 142 262 168 649.0 0 168 263 80 649.0 0 80 264 88 649.0 0 88 265 113 649.0 0 113 266 156 649.0 0 156 267 168 649.0 0 168 268 104 649.0 0 104 269 74 649.0 0 74 270 104 649.0 0 104 271 210 649.0 0 210 272 155 649.0 0 155 273 129 649.0 0 129 274 69 649.0 0 69 275 80 649.0 0 80 276 76 649.0 0 76 277 247 649.0 0 247 278 109 649.0 0 109 279 243 649.0 0 243 280 86 649.0 0 86 281 115 649.0 0 115 282 80 649.0 0 80 283 72 649.0 0 72 284 70 649.0 0 70 285 125 649.0 0 125 286 229 649.0 0 229 287 526 649.0 0 526 288 389 649.0 0 389 289 277 649.0 0 277 290 120 649.0 0 120 291 84 649.0 0 84 292 131 649.0 0 131 293 123 649.0 0 123 294 142 649.0 0 142 295 106 649.0 0 106 296 82 649.0 0 82 297 220 649.0 0 220 298 69 649.0 0 69 299 123 649.0 0 123 300 74 649.0 0 74 301 95 649.0 0 95 Finished in 14.22 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 333,278 Reads with adapters: 18,460 (5.5%) Reads that were too short: 144 (0.0%) Reads written (passing filters): 18,316 (5.5%) Total basepairs processed: 69,464,932 bp Total written (filtered): 3,055,525 bp (4.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18460 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 1 0.0 3 0 1 20 36 0.0 4 35 1 25 1 0.0 4 1 34 56 0.0 4 0 0 0 0 56 51 2 0.0 4 1 0 0 0 1 54 1 0.0 4 1 57 1 0.0 4 1 58 1 0.0 4 0 1 68 2 0.0 4 0 0 0 0 2 69 3 0.0 4 0 0 0 0 3 70 18 0.0 4 0 0 0 0 18 71 18 0.0 4 0 0 0 0 18 72 18158 0.0 4 0 0 0 0 18158 74 1 0.0 4 0 0 0 0 1 75 14 0.0 4 0 0 0 1 13 85 1 0.0 4 0 0 0 0 1 103 1 0.0 4 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 1 119 1 0.0 4 1 120 1 0.0 4 1 121 1 0.0 4 0 1 122 1 0.0 4 1 131 1 0.0 4 0 0 0 1 135 2 0.0 4 1 1 137 2 0.0 4 1 1 138 1 0.0 4 1 139 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 166 4 0.0 4 0 0 0 0 4 168 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 173 67 0.0 4 0 0 0 1 66 174 19 0.0 4 0 0 0 0 19 175 3 0.0 4 0 0 0 0 3 177 4 0.0 4 0 0 0 0 4 178 2 0.0 4 0 0 0 0 2 179 1 0.0 4 0 0 0 0 1 180 2 0.0 4 0 0 0 0 2 181 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 187 3 0.0 4 0 0 0 0 3 195 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 227 5 0.0 4 0 0 0 0 5 228 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 1 2 233 4 0.0 4 0 0 0 0 4 239 1 0.0 4 1 266 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.49 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 18,316 Reads with adapters: 178 (1.0%) Reads written (passing filters): 18,316 (100.0%) Total basepairs processed: 3,055,525 bp Total written (filtered): 3,054,404 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 178 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 91.6% C: 2.2% G: 5.6% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 48 286.2 0 48 4 101 71.5 0 101 5 17 17.9 0 17 9 3 0.1 0 0 3 10 5 0.0 1 0 5 11 2 0.0 1 0 2 169 1 0.0 1 1 220 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.