This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.51 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 93,580 (4.2%) Reads that were too short: 9,459 (0.4%) Reads written (passing filters): 84,121 (3.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 13,330,886 bp (2.0%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 93580 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 473 546.6 0 473 7 3920 546.6 0 3920 8 3902 546.6 0 3902 9 50 546.6 0 50 10 54 546.6 0 54 11 56 546.6 0 56 12 67 546.6 0 67 13 32 546.6 0 32 14 37 546.6 0 37 15 39 546.6 0 39 16 32 546.6 0 32 17 44 546.6 0 44 18 53 546.6 0 53 19 101 546.6 0 101 20 89 546.6 0 89 21 75 546.6 0 75 22 56 546.6 0 56 23 63 546.6 0 63 24 39 546.6 0 39 25 44 546.6 0 44 26 63 546.6 0 63 27 53 546.6 0 53 28 32 546.6 0 32 29 52 546.6 0 52 30 55 546.6 0 55 31 68 546.6 0 68 32 46 546.6 0 46 33 40 546.6 0 40 34 49 546.6 0 49 35 61 546.6 0 61 36 59 546.6 0 59 37 49 546.6 0 49 38 170 546.6 0 170 39 125 546.6 0 125 40 160 546.6 0 160 41 52 546.6 0 52 42 50 546.6 0 50 43 49 546.6 0 49 44 48 546.6 0 48 45 47 546.6 0 47 46 60 546.6 0 60 47 78 546.6 0 78 48 52 546.6 0 52 49 63 546.6 0 63 50 44 546.6 0 44 51 52 546.6 0 52 52 50 546.6 0 50 53 50 546.6 0 50 54 65 546.6 0 65 55 47 546.6 0 47 56 60 546.6 0 60 57 75 546.6 0 75 58 61 546.6 0 61 59 52 546.6 0 52 60 66 546.6 0 66 61 65 546.6 0 65 62 46 546.6 0 46 63 44 546.6 0 44 64 50 546.6 0 50 65 48 546.6 0 48 66 45 546.6 0 45 67 30 546.6 0 30 68 64 546.6 0 64 69 56 546.6 0 56 70 51 546.6 0 51 71 53 546.6 0 53 72 57 546.6 0 57 73 69 546.6 0 69 74 43 546.6 0 43 75 62 546.6 0 62 76 239 546.6 0 239 77 96 546.6 0 96 78 171 546.6 0 171 79 89 546.6 0 89 80 61 546.6 0 61 81 55 546.6 0 55 82 52 546.6 0 52 83 42 546.6 0 42 84 66 546.6 0 66 85 41 546.6 0 41 86 44 546.6 0 44 87 52 546.6 0 52 88 61 546.6 0 61 89 59 546.6 0 59 90 51 546.6 0 51 91 59 546.6 0 59 92 64 546.6 0 64 93 111 546.6 0 111 94 178 546.6 0 178 95 273 546.6 0 273 96 293 546.6 0 293 97 114 546.6 0 114 98 588 546.6 0 588 99 44 546.6 0 44 100 35 546.6 0 35 101 46 546.6 0 46 102 36 546.6 0 36 103 35 546.6 0 35 104 37 546.6 0 37 105 28 546.6 0 28 106 42 546.6 0 42 107 54 546.6 0 54 108 46 546.6 0 46 109 42 546.6 0 42 110 47 546.6 0 47 111 40 546.6 0 40 112 30 546.6 0 30 113 44 546.6 0 44 114 102 546.6 0 102 115 53 546.6 0 53 116 57 546.6 0 57 117 60 546.6 0 60 118 45 546.6 0 45 119 29 546.6 0 29 120 41 546.6 0 41 121 39 546.6 0 39 122 34 546.6 0 34 123 47 546.6 0 47 124 38 546.6 0 38 125 57 546.6 0 57 126 47 546.6 0 47 127 46 546.6 0 46 128 51 546.6 0 51 129 103 546.6 0 103 130 432 546.6 0 432 131 180 546.6 0 180 132 1785 546.6 0 1785 133 57 546.6 0 57 134 86 546.6 0 86 135 53 546.6 0 53 136 124 546.6 0 124 137 75 546.6 0 75 138 111 546.6 0 111 139 35 546.6 0 35 140 42 546.6 0 42 141 38 546.6 0 38 142 148 546.6 0 148 143 118 546.6 0 118 144 139 546.6 0 139 145 146 546.6 0 146 146 38 546.6 0 38 147 51 546.6 0 51 148 38 546.6 0 38 149 57 546.6 0 57 150 39 546.6 0 39 151 60 546.6 0 60 152 93 546.6 0 93 153 54 546.6 0 54 154 69 546.6 0 69 155 49 546.6 0 49 156 59 546.6 0 59 157 114 546.6 0 114 158 82 546.6 0 82 159 50 546.6 0 50 160 49 546.6 0 49 161 57 546.6 0 57 162 96 546.6 0 96 163 108 546.6 0 108 164 137 546.6 0 137 165 256 546.6 0 256 166 348 546.6 0 348 167 631 546.6 0 631 168 1749 546.6 0 1749 169 39460 546.6 0 39460 170 1377 546.6 0 1377 171 17143 546.6 0 17143 172 288 546.6 0 288 173 35 546.6 0 35 174 90 546.6 0 90 175 51 546.6 0 51 176 44 546.6 0 44 177 35 546.6 0 35 178 165 546.6 0 165 179 42 546.6 0 42 180 41 546.6 0 41 181 47 546.6 0 47 182 37 546.6 0 37 183 31 546.6 0 31 184 56 546.6 0 56 185 55 546.6 0 55 186 46 546.6 0 46 187 25 546.6 0 25 188 41 546.6 0 41 189 40 546.6 0 40 190 46 546.6 0 46 191 35 546.6 0 35 192 76 546.6 0 76 193 48 546.6 0 48 194 123 546.6 0 123 195 34 546.6 0 34 196 32 546.6 0 32 197 30 546.6 0 30 198 46 546.6 0 46 199 43 546.6 0 43 200 42 546.6 0 42 201 38 546.6 0 38 202 91 546.6 0 91 203 139 546.6 0 139 204 82 546.6 0 82 205 75 546.6 0 75 206 101 546.6 0 101 207 36 546.6 0 36 208 34 546.6 0 34 209 37 546.6 0 37 210 41 546.6 0 41 211 47 546.6 0 47 212 35 546.6 0 35 213 42 546.6 0 42 214 47 546.6 0 47 215 52 546.6 0 52 216 37 546.6 0 37 217 41 546.6 0 41 218 48 546.6 0 48 219 43 546.6 0 43 220 39 546.6 0 39 221 93 546.6 0 93 222 299 546.6 0 299 223 230 546.6 0 230 224 212 546.6 0 212 225 33 546.6 0 33 226 33 546.6 0 33 227 44 546.6 0 44 228 40 546.6 0 40 229 46 546.6 0 46 230 107 546.6 0 107 231 61 546.6 0 61 232 73 546.6 0 73 233 50 546.6 0 50 234 55 546.6 0 55 235 75 546.6 0 75 236 104 546.6 0 104 237 172 546.6 0 172 238 131 546.6 0 131 239 60 546.6 0 60 240 42 546.6 0 42 241 44 546.6 0 44 242 44 546.6 0 44 243 52 546.6 0 52 244 32 546.6 0 32 245 44 546.6 0 44 246 52 546.6 0 52 247 35 546.6 0 35 248 35 546.6 0 35 249 46 546.6 0 46 250 39 546.6 0 39 251 45 546.6 0 45 252 40 546.6 0 40 253 49 546.6 0 49 254 49 546.6 0 49 255 66 546.6 0 66 256 40 546.6 0 40 257 39 546.6 0 39 258 45 546.6 0 45 259 51 546.6 0 51 260 40 546.6 0 40 261 69 546.6 0 69 262 117 546.6 0 117 263 216 546.6 0 216 264 57 546.6 0 57 265 39 546.6 0 39 266 51 546.6 0 51 267 51 546.6 0 51 268 46 546.6 0 46 269 65 546.6 0 65 270 78 546.6 0 78 271 185 546.6 0 185 272 725 546.6 0 725 273 803 546.6 0 803 274 1036 546.6 0 1036 275 45 546.6 0 45 276 38 546.6 0 38 277 48 546.6 0 48 278 39 546.6 0 39 279 71 546.6 0 71 280 49 546.6 0 49 281 72 546.6 0 72 282 77 546.6 0 77 283 60 546.6 0 60 284 48 546.6 0 48 285 49 546.6 0 49 286 50 546.6 0 50 287 40 546.6 0 40 288 54 546.6 0 54 289 55 546.6 0 55 290 42 546.6 0 42 291 61 546.6 0 61 292 93 546.6 0 93 293 64 546.6 0 64 294 102 546.6 0 102 295 60 546.6 0 60 296 144 546.6 0 144 297 285 546.6 0 285 298 200 546.6 0 200 299 72 546.6 0 72 300 45 546.6 0 45 301 44 546.6 0 44 Finished in 2.93 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 84,121 Reads with adapters: 4,564 (5.4%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 4,557 (5.4%) Total basepairs processed: 13,330,886 bp Total written (filtered): 1,229,234 bp (9.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4564 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 0 10 18 1 0.0 3 0 0 1 19 47 0.0 3 0 45 1 1 20 4057 0.0 4 3991 62 4 21 10 0.0 4 1 9 22 7 0.0 4 0 7 23 267 0.0 4 257 9 0 1 24 1 0.0 4 0 1 25 1 0.0 4 1 26 1 0.0 4 1 41 16 0.0 4 0 0 0 0 16 42 4 0.0 4 0 0 0 0 4 74 2 0.0 4 2 75 5 0.0 4 5 76 8 0.0 4 8 77 3 0.0 4 3 78 2 0.0 4 2 86 2 0.0 4 2 87 4 0.0 4 4 88 2 0.0 4 2 89 4 0.0 4 4 93 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 101 0.0 4 0 0 0 0 101 162 1 0.0 4 0 0 0 0 1 184 3 0.0 4 0 0 0 0 3 209 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 4,557 Reads with adapters: 1,470 (32.3%) Reads written (passing filters): 4,557 (100.0%) Total basepairs processed: 1,229,234 bp Total written (filtered): 913,261 bp (74.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1470 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 28.0% C: 52.2% G: 18.8% T: 0.6% none/other: 0.4% Overview of removed sequences length count expect max.err error counts 3 4 71.2 0 4 4 2 17.8 0 2 9 5 0.0 0 0 5 10 42 0.0 1 0 42 11 242 0.0 1 1 241 170 1 0.0 1 1 258 1 0.0 1 1 259 377 0.0 1 355 22 260 16 0.0 1 14 2 261 10 0.0 1 9 1 269 3 0.0 1 2 1 270 710 0.0 1 666 44 271 33 0.0 1 30 3 272 18 0.0 1 17 1 273 5 0.0 1 4 1 274 1 0.0 1 1