This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.68 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 69,977 (3.1%) Reads that were too short: 7,730 (0.3%) Reads written (passing filters): 62,247 (2.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,077,924 bp (2.5%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 69977 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5255 546.6 0 5255 7 713 546.6 0 713 8 26311 546.6 0 26311 9 19620 546.6 0 19620 10 59 546.6 0 59 11 215 546.6 0 215 12 20 546.6 0 20 13 24 546.6 0 24 14 17 546.6 0 17 15 32 546.6 0 32 16 16 546.6 0 16 17 7 546.6 0 7 18 15 546.6 0 15 19 17 546.6 0 17 20 20 546.6 0 20 21 15 546.6 0 15 22 23 546.6 0 23 23 19 546.6 0 19 24 10 546.6 0 10 25 18 546.6 0 18 26 8 546.6 0 8 27 20 546.6 0 20 28 12 546.6 0 12 29 9 546.6 0 9 30 13 546.6 0 13 31 8 546.6 0 8 32 8 546.6 0 8 33 18 546.6 0 18 34 20 546.6 0 20 35 41 546.6 0 41 36 16 546.6 0 16 37 25 546.6 0 25 38 38 546.6 0 38 39 15 546.6 0 15 40 19 546.6 0 19 41 27 546.6 0 27 42 25 546.6 0 25 43 28 546.6 0 28 44 15 546.6 0 15 45 22 546.6 0 22 46 25 546.6 0 25 47 189 546.6 0 189 48 285 546.6 0 285 49 324 546.6 0 324 50 920 546.6 0 920 51 53 546.6 0 53 52 13 546.6 0 13 53 12 546.6 0 12 54 13 546.6 0 13 55 13 546.6 0 13 56 10 546.6 0 10 57 10 546.6 0 10 58 14 546.6 0 14 59 12 546.6 0 12 60 19 546.6 0 19 61 15 546.6 0 15 62 12 546.6 0 12 63 12 546.6 0 12 64 12 546.6 0 12 65 4 546.6 0 4 66 18 546.6 0 18 67 18 546.6 0 18 68 11 546.6 0 11 69 19 546.6 0 19 70 18 546.6 0 18 71 5 546.6 0 5 72 13 546.6 0 13 73 14 546.6 0 14 74 30 546.6 0 30 75 13 546.6 0 13 76 36 546.6 0 36 77 14 546.6 0 14 78 15 546.6 0 15 79 24 546.6 0 24 80 27 546.6 0 27 81 47 546.6 0 47 82 200 546.6 0 200 83 527 546.6 0 527 84 29 546.6 0 29 85 17 546.6 0 17 86 11 546.6 0 11 87 46 546.6 0 46 88 17 546.6 0 17 89 27 546.6 0 27 90 46 546.6 0 46 91 21 546.6 0 21 92 12 546.6 0 12 93 19 546.6 0 19 94 22 546.6 0 22 95 19 546.6 0 19 96 22 546.6 0 22 97 99 546.6 0 99 98 319 546.6 0 319 99 18 546.6 0 18 100 16 546.6 0 16 101 13 546.6 0 13 102 10 546.6 0 10 103 9 546.6 0 9 104 21 546.6 0 21 105 19 546.6 0 19 106 11 546.6 0 11 107 18 546.6 0 18 108 17 546.6 0 17 109 18 546.6 0 18 110 17 546.6 0 17 111 19 546.6 0 19 112 14 546.6 0 14 113 30 546.6 0 30 114 44 546.6 0 44 115 57 546.6 0 57 116 22 546.6 0 22 117 24 546.6 0 24 118 67 546.6 0 67 119 79 546.6 0 79 120 246 546.6 0 246 121 23 546.6 0 23 122 20 546.6 0 20 123 16 546.6 0 16 124 17 546.6 0 17 125 17 546.6 0 17 126 30 546.6 0 30 127 22 546.6 0 22 128 25 546.6 0 25 129 29 546.6 0 29 130 25 546.6 0 25 131 11 546.6 0 11 132 23 546.6 0 23 133 14 546.6 0 14 134 26 546.6 0 26 135 37 546.6 0 37 136 80 546.6 0 80 137 35 546.6 0 35 138 72 546.6 0 72 139 18 546.6 0 18 140 21 546.6 0 21 141 21 546.6 0 21 142 38 546.6 0 38 143 35 546.6 0 35 144 45 546.6 0 45 145 32 546.6 0 32 146 104 546.6 0 104 147 40 546.6 0 40 148 93 546.6 0 93 149 13 546.6 0 13 150 30 546.6 0 30 151 27 546.6 0 27 152 60 546.6 0 60 153 76 546.6 0 76 154 85 546.6 0 85 155 24 546.6 0 24 156 22 546.6 0 22 157 20 546.6 0 20 158 33 546.6 0 33 159 26 546.6 0 26 160 20 546.6 0 20 161 7 546.6 0 7 162 20 546.6 0 20 163 9 546.6 0 9 164 13 546.6 0 13 165 19 546.6 0 19 166 26 546.6 0 26 167 10 546.6 0 10 168 19 546.6 0 19 169 15 546.6 0 15 170 24 546.6 0 24 171 18 546.6 0 18 172 34 546.6 0 34 173 18 546.6 0 18 174 4 546.6 0 4 175 13 546.6 0 13 176 24 546.6 0 24 177 16 546.6 0 16 178 27 546.6 0 27 179 19 546.6 0 19 180 30 546.6 0 30 181 61 546.6 0 61 182 45 546.6 0 45 183 73 546.6 0 73 184 16 546.6 0 16 185 19 546.6 0 19 186 19 546.6 0 19 187 19 546.6 0 19 188 28 546.6 0 28 189 42 546.6 0 42 190 39 546.6 0 39 191 99 546.6 0 99 192 144 546.6 0 144 193 147 546.6 0 147 194 159 546.6 0 159 195 857 546.6 0 857 196 519 546.6 0 519 197 676 546.6 0 676 198 86 546.6 0 86 199 43 546.6 0 43 200 15 546.6 0 15 201 27 546.6 0 27 202 19 546.6 0 19 203 22 546.6 0 22 204 16 546.6 0 16 205 17 546.6 0 17 206 26 546.6 0 26 207 18 546.6 0 18 208 17 546.6 0 17 209 27 546.6 0 27 210 24 546.6 0 24 211 17 546.6 0 17 212 28 546.6 0 28 213 35 546.6 0 35 214 102 546.6 0 102 215 89 546.6 0 89 216 81 546.6 0 81 217 34 546.6 0 34 218 28 546.6 0 28 219 29 546.6 0 29 220 31 546.6 0 31 221 35 546.6 0 35 222 36 546.6 0 36 223 28 546.6 0 28 224 27 546.6 0 27 225 28 546.6 0 28 226 29 546.6 0 29 227 18 546.6 0 18 228 18 546.6 0 18 229 35 546.6 0 35 230 32 546.6 0 32 231 81 546.6 0 81 232 49 546.6 0 49 233 39 546.6 0 39 234 70 546.6 0 70 235 103 546.6 0 103 236 147 546.6 0 147 237 41 546.6 0 41 238 62 546.6 0 62 239 96 546.6 0 96 240 114 546.6 0 114 241 109 546.6 0 109 242 30 546.6 0 30 243 132 546.6 0 132 244 128 546.6 0 128 245 311 546.6 0 311 246 82 546.6 0 82 247 77 546.6 0 77 248 72 546.6 0 72 249 147 546.6 0 147 250 76 546.6 0 76 251 89 546.6 0 89 252 106 546.6 0 106 253 139 546.6 0 139 254 91 546.6 0 91 255 33 546.6 0 33 256 35 546.6 0 35 257 55 546.6 0 55 258 46 546.6 0 46 259 90 546.6 0 90 260 90 546.6 0 90 261 181 546.6 0 181 262 240 546.6 0 240 263 266 546.6 0 266 264 60 546.6 0 60 265 55 546.6 0 55 266 52 546.6 0 52 267 32 546.6 0 32 268 67 546.6 0 67 269 125 546.6 0 125 270 162 546.6 0 162 271 128 546.6 0 128 272 54 546.6 0 54 273 35 546.6 0 35 274 45 546.6 0 45 275 44 546.6 0 44 276 42 546.6 0 42 277 25 546.6 0 25 278 43 546.6 0 43 279 42 546.6 0 42 280 30 546.6 0 30 281 57 546.6 0 57 282 52 546.6 0 52 283 70 546.6 0 70 284 81 546.6 0 81 285 125 546.6 0 125 286 152 546.6 0 152 287 61 546.6 0 61 288 220 546.6 0 220 289 267 546.6 0 267 290 112 546.6 0 112 291 178 546.6 0 178 292 90 546.6 0 90 293 118 546.6 0 118 294 56 546.6 0 56 295 52 546.6 0 52 296 58 546.6 0 58 297 104 546.6 0 104 298 142 546.6 0 142 299 166 546.6 0 166 300 82 546.6 0 82 301 73 546.6 0 73 Finished in 2.94 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 62,247 Reads with adapters: 30,059 (48.3%) Reads that were too short: 59 (0.1%) Reads written (passing filters): 30,000 (48.2%) Total basepairs processed: 17,077,924 bp Total written (filtered): 8,256,706 bp (48.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30059 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 12 0.0 3 0 0 6 6 16 231 0.0 3 0 180 32 19 17 24708 0.0 3 22939 1430 276 63 18 88 0.0 3 4 63 12 9 19 138 0.0 3 6 36 57 39 20 4659 0.0 3 4234 263 49 113 21 12 0.0 3 1 7 3 1 22 11 0.0 3 0 0 4 7 23 15 0.0 3 0 0 0 15 26 2 0.0 3 0 0 0 2 29 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 31 10 0.0 3 0 0 0 10 33 1 0.0 3 0 0 0 1 34 2 0.0 3 1 0 0 1 35 3 0.0 3 0 0 0 3 38 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 3 0.0 3 0 0 0 3 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 5 0.0 3 0 0 0 5 54 3 0.0 3 0 0 0 3 55 5 0.0 3 0 0 0 5 56 3 0.0 3 0 0 0 3 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 143 22 0.0 3 0 0 0 22 144 5 0.0 3 0 0 0 5 146 5 0.0 3 0 0 0 5 147 4 0.0 3 0 0 1 3 150 3 0.0 3 0 0 1 2 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 5 0.0 3 0 0 0 5 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.05 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 30,000 Reads with adapters: 243 (0.8%) Reads written (passing filters): 30,000 (100.0%) Total basepairs processed: 8,256,706 bp Total written (filtered): 8,255,949 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 243 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.7% C: 21.8% G: 18.5% T: 4.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 220 468.8 0 220 4 18 117.2 0 18 5 5 29.3 0 5