This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.69 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 61,003 (2.7%) Reads that were too short: 3,726 (0.2%) Reads written (passing filters): 57,277 (2.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 16,210,214 bp (2.4%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 61003 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4406 546.6 0 4406 7 645 546.6 0 645 8 23544 546.6 0 23544 9 24061 546.6 0 24061 10 49 546.6 0 49 11 32 546.6 0 32 12 12 546.6 0 12 13 13 546.6 0 13 14 3 546.6 0 3 15 12 546.6 0 12 16 7 546.6 0 7 17 3 546.6 0 3 18 8 546.6 0 8 19 6 546.6 0 6 20 6 546.6 0 6 21 8 546.6 0 8 22 12 546.6 0 12 23 7 546.6 0 7 24 8 546.6 0 8 25 4 546.6 0 4 26 3 546.6 0 3 27 10 546.6 0 10 28 6 546.6 0 6 29 8 546.6 0 8 30 7 546.6 0 7 31 8 546.6 0 8 32 5 546.6 0 5 33 10 546.6 0 10 34 6 546.6 0 6 35 7 546.6 0 7 36 6 546.6 0 6 37 6 546.6 0 6 38 14 546.6 0 14 39 6 546.6 0 6 40 8 546.6 0 8 41 8 546.6 0 8 42 15 546.6 0 15 43 13 546.6 0 13 44 7 546.6 0 7 45 10 546.6 0 10 46 9 546.6 0 9 47 16 546.6 0 16 48 80 546.6 0 80 49 145 546.6 0 145 50 324 546.6 0 324 51 20 546.6 0 20 52 8 546.6 0 8 53 4 546.6 0 4 54 10 546.6 0 10 55 4 546.6 0 4 56 4 546.6 0 4 57 5 546.6 0 5 58 12 546.6 0 12 59 5 546.6 0 5 60 5 546.6 0 5 61 6 546.6 0 6 62 10 546.6 0 10 63 5 546.6 0 5 64 13 546.6 0 13 65 5 546.6 0 5 66 2 546.6 0 2 67 19 546.6 0 19 68 5 546.6 0 5 69 10 546.6 0 10 70 1 546.6 0 1 71 8 546.6 0 8 72 5 546.6 0 5 73 14 546.6 0 14 74 12 546.6 0 12 75 10 546.6 0 10 76 21 546.6 0 21 77 8 546.6 0 8 78 8 546.6 0 8 79 3 546.6 0 3 80 12 546.6 0 12 81 7 546.6 0 7 82 52 546.6 0 52 83 84 546.6 0 84 84 14 546.6 0 14 85 12 546.6 0 12 86 13 546.6 0 13 87 5 546.6 0 5 88 5 546.6 0 5 89 10 546.6 0 10 90 15 546.6 0 15 91 6 546.6 0 6 92 11 546.6 0 11 93 7 546.6 0 7 94 8 546.6 0 8 95 12 546.6 0 12 96 13 546.6 0 13 97 39 546.6 0 39 98 164 546.6 0 164 99 10 546.6 0 10 100 11 546.6 0 11 101 4 546.6 0 4 102 6 546.6 0 6 103 6 546.6 0 6 104 8 546.6 0 8 105 12 546.6 0 12 106 7 546.6 0 7 107 16 546.6 0 16 108 12 546.6 0 12 109 14 546.6 0 14 110 8 546.6 0 8 111 11 546.6 0 11 112 13 546.6 0 13 113 9 546.6 0 9 114 27 546.6 0 27 115 13 546.6 0 13 116 16 546.6 0 16 117 9 546.6 0 9 118 26 546.6 0 26 119 36 546.6 0 36 120 103 546.6 0 103 121 16 546.6 0 16 122 13 546.6 0 13 123 5 546.6 0 5 124 3 546.6 0 3 125 5 546.6 0 5 126 15 546.6 0 15 127 17 546.6 0 17 128 12 546.6 0 12 129 35 546.6 0 35 130 9 546.6 0 9 131 12 546.6 0 12 132 14 546.6 0 14 133 6 546.6 0 6 134 9 546.6 0 9 135 12 546.6 0 12 136 23 546.6 0 23 137 14 546.6 0 14 138 25 546.6 0 25 139 12 546.6 0 12 140 4 546.6 0 4 141 14 546.6 0 14 142 23 546.6 0 23 143 11 546.6 0 11 144 11 546.6 0 11 145 16 546.6 0 16 146 87 546.6 0 87 147 17 546.6 0 17 148 78 546.6 0 78 149 9 546.6 0 9 150 17 546.6 0 17 151 7 546.6 0 7 152 65 546.6 0 65 153 69 546.6 0 69 154 85 546.6 0 85 155 12 546.6 0 12 156 6 546.6 0 6 157 9 546.6 0 9 158 9 546.6 0 9 159 8 546.6 0 8 160 11 546.6 0 11 161 12 546.6 0 12 162 17 546.6 0 17 163 6 546.6 0 6 164 9 546.6 0 9 165 12 546.6 0 12 166 7 546.6 0 7 167 11 546.6 0 11 168 16 546.6 0 16 169 3 546.6 0 3 170 12 546.6 0 12 171 6 546.6 0 6 172 14 546.6 0 14 173 8 546.6 0 8 174 11 546.6 0 11 175 14 546.6 0 14 176 13 546.6 0 13 177 8 546.6 0 8 178 15 546.6 0 15 179 12 546.6 0 12 180 22 546.6 0 22 181 73 546.6 0 73 182 16 546.6 0 16 183 58 546.6 0 58 184 9 546.6 0 9 185 9 546.6 0 9 186 8 546.6 0 8 187 7 546.6 0 7 188 16 546.6 0 16 189 18 546.6 0 18 190 15 546.6 0 15 191 86 546.6 0 86 192 132 546.6 0 132 193 140 546.6 0 140 194 49 546.6 0 49 195 354 546.6 0 354 196 214 546.6 0 214 197 257 546.6 0 257 198 26 546.6 0 26 199 10 546.6 0 10 200 15 546.6 0 15 201 21 546.6 0 21 202 12 546.6 0 12 203 8 546.6 0 8 204 11 546.6 0 11 205 6 546.6 0 6 206 6 546.6 0 6 207 4 546.6 0 4 208 12 546.6 0 12 209 13 546.6 0 13 210 9 546.6 0 9 211 15 546.6 0 15 212 14 546.6 0 14 213 9 546.6 0 9 214 22 546.6 0 22 215 26 546.6 0 26 216 21 546.6 0 21 217 8 546.6 0 8 218 22 546.6 0 22 219 6 546.6 0 6 220 10 546.6 0 10 221 13 546.6 0 13 222 14 546.6 0 14 223 13 546.6 0 13 224 12 546.6 0 12 225 9 546.6 0 9 226 9 546.6 0 9 227 14 546.6 0 14 228 5 546.6 0 5 229 6 546.6 0 6 230 14 546.6 0 14 231 29 546.6 0 29 232 22 546.6 0 22 233 18 546.6 0 18 234 44 546.6 0 44 235 101 546.6 0 101 236 143 546.6 0 143 237 25 546.6 0 25 238 16 546.6 0 16 239 33 546.6 0 33 240 32 546.6 0 32 241 23 546.6 0 23 242 12 546.6 0 12 243 32 546.6 0 32 244 49 546.6 0 49 245 88 546.6 0 88 246 27 546.6 0 27 247 26 546.6 0 26 248 22 546.6 0 22 249 69 546.6 0 69 250 32 546.6 0 32 251 59 546.6 0 59 252 36 546.6 0 36 253 60 546.6 0 60 254 23 546.6 0 23 255 11 546.6 0 11 256 17 546.6 0 17 257 24 546.6 0 24 258 26 546.6 0 26 259 45 546.6 0 45 260 57 546.6 0 57 261 65 546.6 0 65 262 109 546.6 0 109 263 111 546.6 0 111 264 13 546.6 0 13 265 14 546.6 0 14 266 9 546.6 0 9 267 10 546.6 0 10 268 21 546.6 0 21 269 26 546.6 0 26 270 47 546.6 0 47 271 28 546.6 0 28 272 13 546.6 0 13 273 11 546.6 0 11 274 48 546.6 0 48 275 21 546.6 0 21 276 8 546.6 0 8 277 10 546.6 0 10 278 9 546.6 0 9 279 14 546.6 0 14 280 19 546.6 0 19 281 17 546.6 0 17 282 26 546.6 0 26 283 39 546.6 0 39 284 65 546.6 0 65 285 46 546.6 0 46 286 66 546.6 0 66 287 41 546.6 0 41 288 340 546.6 0 340 289 267 546.6 0 267 290 84 546.6 0 84 291 82 546.6 0 82 292 54 546.6 0 54 293 102 546.6 0 102 294 22 546.6 0 22 295 26 546.6 0 26 296 38 546.6 0 38 297 30 546.6 0 30 298 57 546.6 0 57 299 78 546.6 0 78 300 66 546.6 0 66 301 30 546.6 0 30 Finished in 3.00 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 57,277 Reads with adapters: 26,468 (46.2%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 26,459 (46.2%) Total basepairs processed: 16,210,214 bp Total written (filtered): 7,147,043 bp (44.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26468 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 23 0.0 3 0 0 23 19 418 0.0 3 0 411 6 0 1 20 25430 0.0 4 24963 462 5 21 57 0.0 4 16 39 0 2 22 7 0.0 4 4 0 3 23 23 0.0 4 0 0 0 22 1 25 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 7 0.0 4 0 0 0 0 7 127 441 0.0 4 0 0 0 0 441 130 42 0.0 4 0 0 0 0 42 133 1 0.0 4 1 200 1 0.0 4 1 209 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 26,459 Reads with adapters: 2,846 (10.8%) Reads written (passing filters): 26,459 (100.0%) Total basepairs processed: 7,147,043 bp Total written (filtered): 7,117,523 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2846 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 3.1% G: 93.7% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 413.4 0 5 4 3 103.4 0 3 5 1 25.8 0 1 6 2 6.5 0 2 7 2 1.6 0 2 9 725 0.1 0 23 702 10 1838 0.0 1 56 1782 11 254 0.0 1 0 254 12 2 0.0 1 0 2 13 3 0.0 1 0 3 138 1 0.0 1 1 150 7 0.0 1 1 6 152 2 0.0 1 0 2 208 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.