This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.10 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 57,996 (2.6%) Reads that were too short: 24,025 (1.1%) Reads written (passing filters): 33,971 (1.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 6,923,188 bp (1.0%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 57996 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 494 546.6 0 494 7 2178 546.6 0 2178 8 1951 546.6 0 1951 9 55 546.6 0 55 10 118 546.6 0 118 11 28 546.6 0 28 12 38 546.6 0 38 13 33 546.6 0 33 14 58 546.6 0 58 15 27 546.6 0 27 16 30 546.6 0 30 17 54 546.6 0 54 18 67 546.6 0 67 19 72 546.6 0 72 20 32 546.6 0 32 21 34 546.6 0 34 22 19 546.6 0 19 23 43 546.6 0 43 24 29 546.6 0 29 25 41 546.6 0 41 26 43 546.6 0 43 27 25 546.6 0 25 28 33 546.6 0 33 29 41 546.6 0 41 30 36 546.6 0 36 31 36 546.6 0 36 32 25 546.6 0 25 33 33 546.6 0 33 34 22 546.6 0 22 35 36 546.6 0 36 36 25 546.6 0 25 37 21 546.6 0 21 38 27 546.6 0 27 39 40 546.6 0 40 40 32 546.6 0 32 41 34 546.6 0 34 42 38 546.6 0 38 43 43 546.6 0 43 44 38 546.6 0 38 45 29 546.6 0 29 46 57 546.6 0 57 47 47 546.6 0 47 48 82 546.6 0 82 49 76 546.6 0 76 50 38 546.6 0 38 51 39 546.6 0 39 52 44 546.6 0 44 53 76 546.6 0 76 54 74 546.6 0 74 55 35 546.6 0 35 56 42 546.6 0 42 57 38 546.6 0 38 58 31 546.6 0 31 59 45 546.6 0 45 60 88 546.6 0 88 61 62 546.6 0 62 62 107 546.6 0 107 63 78 546.6 0 78 64 76 546.6 0 76 65 54 546.6 0 54 66 55 546.6 0 55 67 77 546.6 0 77 68 58 546.6 0 58 69 62 546.6 0 62 70 119 546.6 0 119 71 89 546.6 0 89 72 93 546.6 0 93 73 418 546.6 0 418 74 640 546.6 0 640 75 1162 546.6 0 1162 76 262 546.6 0 262 77 3133 546.6 0 3133 78 2063 546.6 0 2063 79 2789 546.6 0 2789 80 36 546.6 0 36 81 33 546.6 0 33 82 33 546.6 0 33 83 23 546.6 0 23 84 40 546.6 0 40 85 42 546.6 0 42 86 65 546.6 0 65 87 31 546.6 0 31 88 39 546.6 0 39 89 41 546.6 0 41 90 43 546.6 0 43 91 33 546.6 0 33 92 44 546.6 0 44 93 39 546.6 0 39 94 46 546.6 0 46 95 37 546.6 0 37 96 38 546.6 0 38 97 54 546.6 0 54 98 26 546.6 0 26 99 60 546.6 0 60 100 28 546.6 0 28 101 34 546.6 0 34 102 32 546.6 0 32 103 28 546.6 0 28 104 38 546.6 0 38 105 69 546.6 0 69 106 286 546.6 0 286 107 101 546.6 0 101 108 643 546.6 0 643 109 37 546.6 0 37 110 38 546.6 0 38 111 27 546.6 0 27 112 73 546.6 0 73 113 43 546.6 0 43 114 49 546.6 0 49 115 73 546.6 0 73 116 56 546.6 0 56 117 59 546.6 0 59 118 51 546.6 0 51 119 37 546.6 0 37 120 38 546.6 0 38 121 54 546.6 0 54 122 40 546.6 0 40 123 51 546.6 0 51 124 32 546.6 0 32 125 61 546.6 0 61 126 66 546.6 0 66 127 62 546.6 0 62 128 125 546.6 0 125 129 746 546.6 0 746 130 301 546.6 0 301 131 75 546.6 0 75 132 80 546.6 0 80 133 190 546.6 0 190 134 224 546.6 0 224 135 255 546.6 0 255 136 177 546.6 0 177 137 65 546.6 0 65 138 67 546.6 0 67 139 56 546.6 0 56 140 111 546.6 0 111 141 68 546.6 0 68 142 75 546.6 0 75 143 58 546.6 0 58 144 88 546.6 0 88 145 180 546.6 0 180 146 735 546.6 0 735 147 1602 546.6 0 1602 148 1799 546.6 0 1799 149 58 546.6 0 58 150 46 546.6 0 46 151 56 546.6 0 56 152 45 546.6 0 45 153 50 546.6 0 50 154 61 546.6 0 61 155 235 546.6 0 235 156 197 546.6 0 197 157 191 546.6 0 191 158 98 546.6 0 98 159 61 546.6 0 61 160 69 546.6 0 69 161 77 546.6 0 77 162 40 546.6 0 40 163 43 546.6 0 43 164 98 546.6 0 98 165 134 546.6 0 134 166 197 546.6 0 197 167 113 546.6 0 113 168 150 546.6 0 150 169 92 546.6 0 92 170 62 546.6 0 62 171 37 546.6 0 37 172 31 546.6 0 31 173 48 546.6 0 48 174 54 546.6 0 54 175 34 546.6 0 34 176 25 546.6 0 25 177 49 546.6 0 49 178 44 546.6 0 44 179 60 546.6 0 60 180 69 546.6 0 69 181 61 546.6 0 61 182 62 546.6 0 62 183 44 546.6 0 44 184 279 546.6 0 279 185 334 546.6 0 334 186 577 546.6 0 577 187 115 546.6 0 115 188 61 546.6 0 61 189 40 546.6 0 40 190 42 546.6 0 42 191 49 546.6 0 49 192 60 546.6 0 60 193 46 546.6 0 46 194 58 546.6 0 58 195 179 546.6 0 179 196 181 546.6 0 181 197 292 546.6 0 292 198 134 546.6 0 134 199 199 546.6 0 199 200 116 546.6 0 116 201 65 546.6 0 65 202 42 546.6 0 42 203 53 546.6 0 53 204 168 546.6 0 168 205 60 546.6 0 60 206 90 546.6 0 90 207 55 546.6 0 55 208 70 546.6 0 70 209 56 546.6 0 56 210 50 546.6 0 50 211 84 546.6 0 84 212 151 546.6 0 151 213 60 546.6 0 60 214 62 546.6 0 62 215 94 546.6 0 94 216 133 546.6 0 133 217 182 546.6 0 182 218 82 546.6 0 82 219 97 546.6 0 97 220 73 546.6 0 73 221 93 546.6 0 93 222 56 546.6 0 56 223 46 546.6 0 46 224 69 546.6 0 69 225 104 546.6 0 104 226 105 546.6 0 105 227 90 546.6 0 90 228 58 546.6 0 58 229 65 546.6 0 65 230 54 546.6 0 54 231 61 546.6 0 61 232 75 546.6 0 75 233 111 546.6 0 111 234 174 546.6 0 174 235 372 546.6 0 372 236 3103 546.6 0 3103 237 1271 546.6 0 1271 238 3749 546.6 0 3749 239 153 546.6 0 153 240 74 546.6 0 74 241 106 546.6 0 106 242 74 546.6 0 74 243 49 546.6 0 49 244 67 546.6 0 67 245 78 546.6 0 78 246 228 546.6 0 228 247 374 546.6 0 374 248 401 546.6 0 401 249 189 546.6 0 189 250 184 546.6 0 184 251 493 546.6 0 493 252 440 546.6 0 440 253 310 546.6 0 310 254 161 546.6 0 161 255 243 546.6 0 243 256 128 546.6 0 128 257 105 546.6 0 105 258 85 546.6 0 85 259 93 546.6 0 93 260 60 546.6 0 60 261 68 546.6 0 68 262 48 546.6 0 48 263 69 546.6 0 69 264 75 546.6 0 75 265 86 546.6 0 86 266 78 546.6 0 78 267 80 546.6 0 80 268 70 546.6 0 70 269 68 546.6 0 68 270 83 546.6 0 83 271 93 546.6 0 93 272 101 546.6 0 101 273 113 546.6 0 113 274 119 546.6 0 119 275 84 546.6 0 84 276 64 546.6 0 64 277 90 546.6 0 90 278 215 546.6 0 215 279 147 546.6 0 147 280 382 546.6 0 382 281 350 546.6 0 350 282 275 546.6 0 275 283 259 546.6 0 259 284 321 546.6 0 321 285 229 546.6 0 229 286 329 546.6 0 329 287 152 546.6 0 152 288 127 546.6 0 127 289 136 546.6 0 136 290 105 546.6 0 105 291 121 546.6 0 121 292 94 546.6 0 94 293 163 546.6 0 163 294 198 546.6 0 198 295 412 546.6 0 412 296 1054 546.6 0 1054 297 808 546.6 0 808 298 662 546.6 0 662 299 238 546.6 0 238 300 132 546.6 0 132 301 314 546.6 0 314 Finished in 1.28 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 33,971 Reads with adapters: 2,820 (8.3%) Reads that were too short: 124 (0.4%) Reads written (passing filters): 2,696 (7.9%) Total basepairs processed: 6,923,188 bp Total written (filtered): 661,552 bp (9.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2820 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 17 0.0 3 0 14 3 17 2129 0.0 3 1954 145 27 3 18 10 0.0 3 1 6 0 3 19 5 0.0 3 0 1 2 2 20 14 0.0 3 3 0 0 11 23 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 39 29 0.0 3 0 0 0 29 40 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 4 0.0 3 0 0 0 4 44 1 0.0 3 0 0 0 1 45 57 0.0 3 0 0 0 57 46 15 0.0 3 0 0 0 15 48 8 0.0 3 0 0 0 8 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 61 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 20 0.0 3 0 0 0 20 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 1 1 88 6 0.0 3 0 0 0 6 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 96 3 0.0 3 0 0 2 1 98 4 0.0 3 0 0 0 4 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 6 0.0 3 0 0 0 6 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 228 0.0 3 0 0 2 226 115 33 0.0 3 0 0 0 33 116 3 0.0 3 0 0 0 3 117 40 0.0 3 0 0 0 40 118 21 0.0 3 0 0 0 21 119 3 0.0 3 0 0 0 3 120 2 0.0 3 0 0 0 2 121 5 0.0 3 0 0 0 5 122 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 5 0.0 3 0 0 0 5 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 7 0.0 3 0 0 0 7 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 215 17 0.0 3 0 0 2 15 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 1 258 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 2,696 Reads with adapters: 21 (0.8%) Reads written (passing filters): 2,696 (100.0%) Total basepairs processed: 661,552 bp Total written (filtered): 661,487 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 21 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.8% C: 38.1% G: 33.3% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 42.1 0 20 5 1 2.6 0 1