This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.35 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 65,162 (2.9%) Reads that were too short: 22,854 (1.0%) Reads written (passing filters): 42,308 (1.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 10,944,394 bp (1.6%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 65162 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 533 546.6 0 533 7 15436 546.6 0 15436 8 224 546.6 0 224 9 14607 546.6 0 14607 10 14 546.6 0 14 11 13 546.6 0 13 12 11 546.6 0 11 13 6 546.6 0 6 14 9 546.6 0 9 15 7 546.6 0 7 16 7 546.6 0 7 17 9 546.6 0 9 18 11 546.6 0 11 19 11 546.6 0 11 20 4 546.6 0 4 21 11 546.6 0 11 22 8 546.6 0 8 23 4 546.6 0 4 24 13 546.6 0 13 25 6 546.6 0 6 26 11 546.6 0 11 27 9 546.6 0 9 28 6 546.6 0 6 29 5 546.6 0 5 30 4 546.6 0 4 31 15 546.6 0 15 32 4 546.6 0 4 33 5 546.6 0 5 34 15 546.6 0 15 35 18 546.6 0 18 36 25 546.6 0 25 37 17 546.6 0 17 38 10 546.6 0 10 39 18 546.6 0 18 40 4 546.6 0 4 41 10 546.6 0 10 42 11 546.6 0 11 43 15 546.6 0 15 44 6 546.6 0 6 45 15 546.6 0 15 46 11 546.6 0 11 47 7 546.6 0 7 48 11 546.6 0 11 49 8 546.6 0 8 50 5 546.6 0 5 51 7 546.6 0 7 52 8 546.6 0 8 53 9 546.6 0 9 54 11 546.6 0 11 55 521 546.6 0 521 56 48 546.6 0 48 57 10 546.6 0 10 58 6 546.6 0 6 59 9 546.6 0 9 60 6 546.6 0 6 61 17 546.6 0 17 62 11 546.6 0 11 63 5 546.6 0 5 64 10 546.6 0 10 65 13 546.6 0 13 66 20 546.6 0 20 67 6 546.6 0 6 68 10 546.6 0 10 69 14 546.6 0 14 70 43 546.6 0 43 71 24 546.6 0 24 72 44 546.6 0 44 73 16 546.6 0 16 74 12 546.6 0 12 75 22 546.6 0 22 76 41 546.6 0 41 77 40 546.6 0 40 78 35 546.6 0 35 79 29 546.6 0 29 80 55 546.6 0 55 81 151 546.6 0 151 82 59 546.6 0 59 83 81 546.6 0 81 84 23 546.6 0 23 85 33 546.6 0 33 86 23 546.6 0 23 87 20 546.6 0 20 88 18 546.6 0 18 89 20 546.6 0 20 90 29 546.6 0 29 91 17 546.6 0 17 92 25 546.6 0 25 93 19 546.6 0 19 94 21 546.6 0 21 95 56 546.6 0 56 96 20 546.6 0 20 97 25 546.6 0 25 98 35 546.6 0 35 99 21 546.6 0 21 100 26 546.6 0 26 101 77 546.6 0 77 102 138 546.6 0 138 103 240 546.6 0 240 104 97 546.6 0 97 105 44 546.6 0 44 106 45 546.6 0 45 107 34 546.6 0 34 108 50 546.6 0 50 109 55 546.6 0 55 110 68 546.6 0 68 111 75 546.6 0 75 112 90 546.6 0 90 113 67 546.6 0 67 114 43 546.6 0 43 115 44 546.6 0 44 116 43 546.6 0 43 117 39 546.6 0 39 118 82 546.6 0 82 119 72 546.6 0 72 120 42 546.6 0 42 121 43 546.6 0 43 122 101 546.6 0 101 123 71 546.6 0 71 124 157 546.6 0 157 125 58 546.6 0 58 126 52 546.6 0 52 127 65 546.6 0 65 128 45 546.6 0 45 129 49 546.6 0 49 130 32 546.6 0 32 131 78 546.6 0 78 132 83 546.6 0 83 133 197 546.6 0 197 134 124 546.6 0 124 135 165 546.6 0 165 136 77 546.6 0 77 137 151 546.6 0 151 138 405 546.6 0 405 139 169 546.6 0 169 140 104 546.6 0 104 141 76 546.6 0 76 142 40 546.6 0 40 143 56 546.6 0 56 144 75 546.6 0 75 145 75 546.6 0 75 146 200 546.6 0 200 147 130 546.6 0 130 148 285 546.6 0 285 149 73 546.6 0 73 150 59 546.6 0 59 151 86 546.6 0 86 152 57 546.6 0 57 153 72 546.6 0 72 154 72 546.6 0 72 155 69 546.6 0 69 156 166 546.6 0 166 157 100 546.6 0 100 158 137 546.6 0 137 159 111 546.6 0 111 160 94 546.6 0 94 161 90 546.6 0 90 162 82 546.6 0 82 163 62 546.6 0 62 164 70 546.6 0 70 165 94 546.6 0 94 166 65 546.6 0 65 167 434 546.6 0 434 168 56 546.6 0 56 169 100 546.6 0 100 170 44 546.6 0 44 171 51 546.6 0 51 172 68 546.6 0 68 173 74 546.6 0 74 174 42 546.6 0 42 175 36 546.6 0 36 176 28 546.6 0 28 177 37 546.6 0 37 178 37 546.6 0 37 179 76 546.6 0 76 180 236 546.6 0 236 181 312 546.6 0 312 182 230 546.6 0 230 183 51 546.6 0 51 184 62 546.6 0 62 185 91 546.6 0 91 186 45 546.6 0 45 187 43 546.6 0 43 188 86 546.6 0 86 189 49 546.6 0 49 190 97 546.6 0 97 191 48 546.6 0 48 192 51 546.6 0 51 193 61 546.6 0 61 194 62 546.6 0 62 195 47 546.6 0 47 196 50 546.6 0 50 197 51 546.6 0 51 198 91 546.6 0 91 199 114 546.6 0 114 200 97 546.6 0 97 201 51 546.6 0 51 202 36 546.6 0 36 203 49 546.6 0 49 204 73 546.6 0 73 205 114 546.6 0 114 206 108 546.6 0 108 207 90 546.6 0 90 208 115 546.6 0 115 209 133 546.6 0 133 210 320 546.6 0 320 211 2514 546.6 0 2514 212 3469 546.6 0 3469 213 2562 546.6 0 2562 214 1654 546.6 0 1654 215 129 546.6 0 129 216 54 546.6 0 54 217 57 546.6 0 57 218 87 546.6 0 87 219 83 546.6 0 83 220 72 546.6 0 72 221 59 546.6 0 59 222 58 546.6 0 58 223 96 546.6 0 96 224 164 546.6 0 164 225 180 546.6 0 180 226 162 546.6 0 162 227 70 546.6 0 70 228 62 546.6 0 62 229 68 546.6 0 68 230 50 546.6 0 50 231 60 546.6 0 60 232 42 546.6 0 42 233 62 546.6 0 62 234 76 546.6 0 76 235 59 546.6 0 59 236 63 546.6 0 63 237 63 546.6 0 63 238 69 546.6 0 69 239 73 546.6 0 73 240 76 546.6 0 76 241 83 546.6 0 83 242 79 546.6 0 79 243 54 546.6 0 54 244 86 546.6 0 86 245 87 546.6 0 87 246 121 546.6 0 121 247 50 546.6 0 50 248 38 546.6 0 38 249 40 546.6 0 40 250 44 546.6 0 44 251 77 546.6 0 77 252 64 546.6 0 64 253 68 546.6 0 68 254 96 546.6 0 96 255 101 546.6 0 101 256 185 546.6 0 185 257 107 546.6 0 107 258 88 546.6 0 88 259 76 546.6 0 76 260 85 546.6 0 85 261 76 546.6 0 76 262 84 546.6 0 84 263 66 546.6 0 66 264 71 546.6 0 71 265 71 546.6 0 71 266 131 546.6 0 131 267 186 546.6 0 186 268 136 546.6 0 136 269 115 546.6 0 115 270 174 546.6 0 174 271 218 546.6 0 218 272 662 546.6 0 662 273 148 546.6 0 148 274 208 546.6 0 208 275 135 546.6 0 135 276 143 546.6 0 143 277 168 546.6 0 168 278 94 546.6 0 94 279 222 546.6 0 222 280 143 546.6 0 143 281 83 546.6 0 83 282 68 546.6 0 68 283 53 546.6 0 53 284 63 546.6 0 63 285 60 546.6 0 60 286 83 546.6 0 83 287 85 546.6 0 85 288 125 546.6 0 125 289 137 546.6 0 137 290 90 546.6 0 90 291 93 546.6 0 93 292 357 546.6 0 357 293 690 546.6 0 690 294 1452 546.6 0 1452 295 403 546.6 0 403 296 129 546.6 0 129 297 69 546.6 0 69 298 75 546.6 0 75 299 155 546.6 0 155 300 138 546.6 0 138 301 235 546.6 0 235 Finished in 1.92 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 42,308 Reads with adapters: 15,103 (35.7%) Reads that were too short: 57 (0.1%) Reads written (passing filters): 15,046 (35.6%) Total basepairs processed: 10,944,394 bp Total written (filtered): 4,128,247 bp (37.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15103 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 12 0.0 3 0 0 9 3 16 254 0.0 3 0 233 16 5 17 14599 0.0 3 13637 802 136 24 18 46 0.0 3 0 28 3 15 19 36 0.0 3 0 1 22 13 20 30 0.0 3 1 0 0 29 21 5 0.0 3 1 1 0 3 25 2 0.0 3 0 0 0 2 33 4 0.0 3 0 0 0 4 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 54 11 0.0 3 0 0 2 9 55 9 0.0 3 0 0 0 9 57 1 0.0 3 0 0 0 1 59 8 0.0 3 0 0 0 8 62 3 0.0 3 1 1 0 1 64 2 0.0 3 0 0 0 2 69 6 0.0 3 0 0 0 6 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 112 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 1 149 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 196 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 1 4 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 15,046 Reads with adapters: 935 (6.2%) Reads written (passing filters): 15,046 (100.0%) Total basepairs processed: 4,128,247 bp Total written (filtered): 4,125,417 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 935 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.5% C: 19.8% G: 12.1% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 910 235.1 0 910 4 25 58.8 0 25