This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.42 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 63,384 (2.8%) Reads that were too short: 20,684 (0.9%) Reads written (passing filters): 42,700 (1.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 11,178,850 bp (1.7%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 63384 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 340 546.6 0 340 7 18034 546.6 0 18034 8 181 546.6 0 181 9 13724 546.6 0 13724 10 14 546.6 0 14 11 10 546.6 0 10 12 10 546.6 0 10 13 15 546.6 0 15 14 11 546.6 0 11 15 8 546.6 0 8 16 15 546.6 0 15 17 10 546.6 0 10 18 9 546.6 0 9 19 19 546.6 0 19 20 6 546.6 0 6 21 24 546.6 0 24 22 18 546.6 0 18 23 11 546.6 0 11 24 14 546.6 0 14 25 11 546.6 0 11 26 10 546.6 0 10 27 18 546.6 0 18 28 8 546.6 0 8 29 12 546.6 0 12 30 11 546.6 0 11 31 6 546.6 0 6 32 15 546.6 0 15 33 15 546.6 0 15 34 11 546.6 0 11 35 13 546.6 0 13 36 15 546.6 0 15 37 9 546.6 0 9 38 12 546.6 0 12 39 13 546.6 0 13 40 14 546.6 0 14 41 12 546.6 0 12 42 17 546.6 0 17 43 13 546.6 0 13 44 18 546.6 0 18 45 9 546.6 0 9 46 5 546.6 0 5 47 18 546.6 0 18 48 23 546.6 0 23 49 3 546.6 0 3 50 7 546.6 0 7 51 9 546.6 0 9 52 7 546.6 0 7 53 8 546.6 0 8 54 10 546.6 0 10 55 14 546.6 0 14 56 11 546.6 0 11 57 10 546.6 0 10 58 7 546.6 0 7 59 6 546.6 0 6 60 13 546.6 0 13 61 8 546.6 0 8 62 8 546.6 0 8 63 10 546.6 0 10 64 11 546.6 0 11 65 21 546.6 0 21 66 19 546.6 0 19 67 4 546.6 0 4 68 12 546.6 0 12 69 8 546.6 0 8 70 24 546.6 0 24 71 15 546.6 0 15 72 27 546.6 0 27 73 12 546.6 0 12 74 15 546.6 0 15 75 13 546.6 0 13 76 11 546.6 0 11 77 13 546.6 0 13 78 36 546.6 0 36 79 37 546.6 0 37 80 39 546.6 0 39 81 190 546.6 0 190 82 70 546.6 0 70 83 99 546.6 0 99 84 38 546.6 0 38 85 63 546.6 0 63 86 63 546.6 0 63 87 57 546.6 0 57 88 36 546.6 0 36 89 32 546.6 0 32 90 39 546.6 0 39 91 39 546.6 0 39 92 32 546.6 0 32 93 40 546.6 0 40 94 46 546.6 0 46 95 46 546.6 0 46 96 33 546.6 0 33 97 24 546.6 0 24 98 35 546.6 0 35 99 21 546.6 0 21 100 23 546.6 0 23 101 47 546.6 0 47 102 98 546.6 0 98 103 104 546.6 0 104 104 75 546.6 0 75 105 34 546.6 0 34 106 34 546.6 0 34 107 45 546.6 0 45 108 37 546.6 0 37 109 41 546.6 0 41 110 65 546.6 0 65 111 60 546.6 0 60 112 73 546.6 0 73 113 45 546.6 0 45 114 46 546.6 0 46 115 63 546.6 0 63 116 30 546.6 0 30 117 44 546.6 0 44 118 80 546.6 0 80 119 72 546.6 0 72 120 55 546.6 0 55 121 35 546.6 0 35 122 59 546.6 0 59 123 58 546.6 0 58 124 128 546.6 0 128 125 74 546.6 0 74 126 53 546.6 0 53 127 71 546.6 0 71 128 29 546.6 0 29 129 39 546.6 0 39 130 51 546.6 0 51 131 49 546.6 0 49 132 58 546.6 0 58 133 128 546.6 0 128 134 115 546.6 0 115 135 149 546.6 0 149 136 74 546.6 0 74 137 144 546.6 0 144 138 413 546.6 0 413 139 156 546.6 0 156 140 106 546.6 0 106 141 68 546.6 0 68 142 32 546.6 0 32 143 54 546.6 0 54 144 93 546.6 0 93 145 86 546.6 0 86 146 284 546.6 0 284 147 109 546.6 0 109 148 253 546.6 0 253 149 70 546.6 0 70 150 40 546.6 0 40 151 71 546.6 0 71 152 67 546.6 0 67 153 83 546.6 0 83 154 84 546.6 0 84 155 67 546.6 0 67 156 134 546.6 0 134 157 87 546.6 0 87 158 127 546.6 0 127 159 71 546.6 0 71 160 60 546.6 0 60 161 60 546.6 0 60 162 49 546.6 0 49 163 51 546.6 0 51 164 86 546.6 0 86 165 72 546.6 0 72 166 82 546.6 0 82 167 535 546.6 0 535 168 63 546.6 0 63 169 140 546.6 0 140 170 54 546.6 0 54 171 78 546.6 0 78 172 72 546.6 0 72 173 66 546.6 0 66 174 50 546.6 0 50 175 40 546.6 0 40 176 40 546.6 0 40 177 37 546.6 0 37 178 45 546.6 0 45 179 76 546.6 0 76 180 172 546.6 0 172 181 140 546.6 0 140 182 145 546.6 0 145 183 46 546.6 0 46 184 67 546.6 0 67 185 77 546.6 0 77 186 64 546.6 0 64 187 60 546.6 0 60 188 70 546.6 0 70 189 54 546.6 0 54 190 99 546.6 0 99 191 62 546.6 0 62 192 63 546.6 0 63 193 54 546.6 0 54 194 49 546.6 0 49 195 57 546.6 0 57 196 47 546.6 0 47 197 52 546.6 0 52 198 77 546.6 0 77 199 76 546.6 0 76 200 88 546.6 0 88 201 53 546.6 0 53 202 59 546.6 0 59 203 61 546.6 0 61 204 58 546.6 0 58 205 86 546.6 0 86 206 90 546.6 0 90 207 92 546.6 0 92 208 112 546.6 0 112 209 130 546.6 0 130 210 290 546.6 0 290 211 2736 546.6 0 2736 212 3683 546.6 0 3683 213 2765 546.6 0 2765 214 1588 546.6 0 1588 215 141 546.6 0 141 216 55 546.6 0 55 217 59 546.6 0 59 218 92 546.6 0 92 219 75 546.6 0 75 220 69 546.6 0 69 221 56 546.6 0 56 222 55 546.6 0 55 223 108 546.6 0 108 224 159 546.6 0 159 225 174 546.6 0 174 226 201 546.6 0 201 227 46 546.6 0 46 228 44 546.6 0 44 229 62 546.6 0 62 230 51 546.6 0 51 231 46 546.6 0 46 232 50 546.6 0 50 233 59 546.6 0 59 234 67 546.6 0 67 235 76 546.6 0 76 236 74 546.6 0 74 237 59 546.6 0 59 238 57 546.6 0 57 239 77 546.6 0 77 240 75 546.6 0 75 241 79 546.6 0 79 242 70 546.6 0 70 243 76 546.6 0 76 244 92 546.6 0 92 245 83 546.6 0 83 246 143 546.6 0 143 247 73 546.6 0 73 248 45 546.6 0 45 249 51 546.6 0 51 250 43 546.6 0 43 251 53 546.6 0 53 252 61 546.6 0 61 253 66 546.6 0 66 254 52 546.6 0 52 255 73 546.6 0 73 256 97 546.6 0 97 257 88 546.6 0 88 258 71 546.6 0 71 259 69 546.6 0 69 260 76 546.6 0 76 261 58 546.6 0 58 262 72 546.6 0 72 263 60 546.6 0 60 264 52 546.6 0 52 265 65 546.6 0 65 266 92 546.6 0 92 267 102 546.6 0 102 268 95 546.6 0 95 269 56 546.6 0 56 270 104 546.6 0 104 271 133 546.6 0 133 272 394 546.6 0 394 273 124 546.6 0 124 274 120 546.6 0 120 275 98 546.6 0 98 276 88 546.6 0 88 277 130 546.6 0 130 278 79 546.6 0 79 279 128 546.6 0 128 280 86 546.6 0 86 281 73 546.6 0 73 282 57 546.6 0 57 283 65 546.6 0 65 284 83 546.6 0 83 285 77 546.6 0 77 286 82 546.6 0 82 287 79 546.6 0 79 288 119 546.6 0 119 289 102 546.6 0 102 290 77 546.6 0 77 291 76 546.6 0 76 292 266 546.6 0 266 293 408 546.6 0 408 294 789 546.6 0 789 295 242 546.6 0 242 296 95 546.6 0 95 297 70 546.6 0 70 298 63 546.6 0 63 299 99 546.6 0 99 300 121 546.6 0 121 301 207 546.6 0 207 Finished in 2.11 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 42,700 Reads with adapters: 18,429 (43.2%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 18,413 (43.1%) Total basepairs processed: 11,178,850 bp Total written (filtered): 5,018,570 bp (44.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18429 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 153 0.0 3 0 148 4 1 20 17958 0.0 4 17552 384 18 4 21 36 0.0 4 6 30 22 4 0.0 4 2 0 2 23 10 0.0 4 0 0 1 8 1 24 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 65 2 0.0 4 1 1 66 2 0.0 4 1 1 67 2 0.0 4 2 68 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 241 0.0 4 0 0 0 0 241 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 18,413 Reads with adapters: 555 (3.0%) Reads written (passing filters): 18,413 (100.0%) Total basepairs processed: 5,018,570 bp Total written (filtered): 5,011,894 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 555 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.5% C: 5.2% G: 87.9% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 23 287.7 0 23 4 4 71.9 0 4 9 2 0.1 0 0 2 10 4 0.0 1 1 3 11 483 0.0 1 5 478 12 34 0.0 1 1 33 15 1 0.0 1 0 1 152 1 0.0 1 0 1 210 2 0.0 1 2 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.