This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.70 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 70,739 (3.2%) Reads that were too short: 24,884 (1.1%) Reads written (passing filters): 45,855 (2.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 12,101,164 bp (1.8%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 70739 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 564 546.6 0 564 7 16854 546.6 0 16854 8 15717 546.6 0 15717 9 50 546.6 0 50 10 44 546.6 0 44 11 31 546.6 0 31 12 34 546.6 0 34 13 38 546.6 0 38 14 26 546.6 0 26 15 32 546.6 0 32 16 15 546.6 0 15 17 19 546.6 0 19 18 32 546.6 0 32 19 41 546.6 0 41 20 37 546.6 0 37 21 37 546.6 0 37 22 41 546.6 0 41 23 40 546.6 0 40 24 32 546.6 0 32 25 30 546.6 0 30 26 41 546.6 0 41 27 31 546.6 0 31 28 33 546.6 0 33 29 18 546.6 0 18 30 17 546.6 0 17 31 31 546.6 0 31 32 41 546.6 0 41 33 22 546.6 0 22 34 29 546.6 0 29 35 25 546.6 0 25 36 30 546.6 0 30 37 27 546.6 0 27 38 57 546.6 0 57 39 117 546.6 0 117 40 293 546.6 0 293 41 947 546.6 0 947 42 39 546.6 0 39 43 98 546.6 0 98 44 31 546.6 0 31 45 23 546.6 0 23 46 31 546.6 0 31 47 54 546.6 0 54 48 27 546.6 0 27 49 23 546.6 0 23 50 31 546.6 0 31 51 49 546.6 0 49 52 82 546.6 0 82 53 39 546.6 0 39 54 48 546.6 0 48 55 21 546.6 0 21 56 27 546.6 0 27 57 37 546.6 0 37 58 44 546.6 0 44 59 30 546.6 0 30 60 45 546.6 0 45 61 37 546.6 0 37 62 24 546.6 0 24 63 28 546.6 0 28 64 24 546.6 0 24 65 43 546.6 0 43 66 33 546.6 0 33 67 27 546.6 0 27 68 33 546.6 0 33 69 33 546.6 0 33 70 51 546.6 0 51 71 51 546.6 0 51 72 61 546.6 0 61 73 29 546.6 0 29 74 68 546.6 0 68 75 30 546.6 0 30 76 27 546.6 0 27 77 33 546.6 0 33 78 25 546.6 0 25 79 29 546.6 0 29 80 38 546.6 0 38 81 29 546.6 0 29 82 40 546.6 0 40 83 46 546.6 0 46 84 71 546.6 0 71 85 57 546.6 0 57 86 50 546.6 0 50 87 30 546.6 0 30 88 23 546.6 0 23 89 36 546.6 0 36 90 49 546.6 0 49 91 50 546.6 0 50 92 57 546.6 0 57 93 94 546.6 0 94 94 47 546.6 0 47 95 36 546.6 0 36 96 53 546.6 0 53 97 48 546.6 0 48 98 175 546.6 0 175 99 206 546.6 0 206 100 351 546.6 0 351 101 75 546.6 0 75 102 89 546.6 0 89 103 31 546.6 0 31 104 38 546.6 0 38 105 28 546.6 0 28 106 29 546.6 0 29 107 41 546.6 0 41 108 38 546.6 0 38 109 37 546.6 0 37 110 55 546.6 0 55 111 76 546.6 0 76 112 132 546.6 0 132 113 212 546.6 0 212 114 41 546.6 0 41 115 49 546.6 0 49 116 38 546.6 0 38 117 41 546.6 0 41 118 52 546.6 0 52 119 34 546.6 0 34 120 54 546.6 0 54 121 40 546.6 0 40 122 34 546.6 0 34 123 47 546.6 0 47 124 56 546.6 0 56 125 56 546.6 0 56 126 33 546.6 0 33 127 28 546.6 0 28 128 21 546.6 0 21 129 33 546.6 0 33 130 50 546.6 0 50 131 32 546.6 0 32 132 74 546.6 0 74 133 81 546.6 0 81 134 75 546.6 0 75 135 193 546.6 0 193 136 48 546.6 0 48 137 72 546.6 0 72 138 65 546.6 0 65 139 76 546.6 0 76 140 30 546.6 0 30 141 39 546.6 0 39 142 52 546.6 0 52 143 86 546.6 0 86 144 80 546.6 0 80 145 58 546.6 0 58 146 51 546.6 0 51 147 54 546.6 0 54 148 305 546.6 0 305 149 50 546.6 0 50 150 50 546.6 0 50 151 45 546.6 0 45 152 38 546.6 0 38 153 40 546.6 0 40 154 54 546.6 0 54 155 48 546.6 0 48 156 62 546.6 0 62 157 60 546.6 0 60 158 227 546.6 0 227 159 84 546.6 0 84 160 212 546.6 0 212 161 30 546.6 0 30 162 55 546.6 0 55 163 60 546.6 0 60 164 56 546.6 0 56 165 50 546.6 0 50 166 56 546.6 0 56 167 56 546.6 0 56 168 46 546.6 0 46 169 61 546.6 0 61 170 90 546.6 0 90 171 98 546.6 0 98 172 95 546.6 0 95 173 38 546.6 0 38 174 34 546.6 0 34 175 36 546.6 0 36 176 52 546.6 0 52 177 135 546.6 0 135 178 159 546.6 0 159 179 147 546.6 0 147 180 94 546.6 0 94 181 48 546.6 0 48 182 58 546.6 0 58 183 227 546.6 0 227 184 124 546.6 0 124 185 222 546.6 0 222 186 72 546.6 0 72 187 62 546.6 0 62 188 108 546.6 0 108 189 173 546.6 0 173 190 103 546.6 0 103 191 57 546.6 0 57 192 78 546.6 0 78 193 84 546.6 0 84 194 51 546.6 0 51 195 58 546.6 0 58 196 52 546.6 0 52 197 44 546.6 0 44 198 55 546.6 0 55 199 70 546.6 0 70 200 55 546.6 0 55 201 53 546.6 0 53 202 54 546.6 0 54 203 63 546.6 0 63 204 59 546.6 0 59 205 50 546.6 0 50 206 47 546.6 0 47 207 57 546.6 0 57 208 68 546.6 0 68 209 68 546.6 0 68 210 132 546.6 0 132 211 189 546.6 0 189 212 118 546.6 0 118 213 136 546.6 0 136 214 596 546.6 0 596 215 633 546.6 0 633 216 1613 546.6 0 1613 217 168 546.6 0 168 218 55 546.6 0 55 219 61 546.6 0 61 220 67 546.6 0 67 221 58 546.6 0 58 222 109 546.6 0 109 223 716 546.6 0 716 224 249 546.6 0 249 225 1078 546.6 0 1078 226 72 546.6 0 72 227 56 546.6 0 56 228 90 546.6 0 90 229 54 546.6 0 54 230 71 546.6 0 71 231 48 546.6 0 48 232 60 546.6 0 60 233 51 546.6 0 51 234 52 546.6 0 52 235 55 546.6 0 55 236 66 546.6 0 66 237 82 546.6 0 82 238 113 546.6 0 113 239 129 546.6 0 129 240 118 546.6 0 118 241 144 546.6 0 144 242 137 546.6 0 137 243 57 546.6 0 57 244 55 546.6 0 55 245 72 546.6 0 72 246 42 546.6 0 42 247 89 546.6 0 89 248 56 546.6 0 56 249 59 546.6 0 59 250 86 546.6 0 86 251 265 546.6 0 265 252 60 546.6 0 60 253 68 546.6 0 68 254 55 546.6 0 55 255 82 546.6 0 82 256 92 546.6 0 92 257 120 546.6 0 120 258 155 546.6 0 155 259 104 546.6 0 104 260 107 546.6 0 107 261 64 546.6 0 64 262 101 546.6 0 101 263 249 546.6 0 249 264 375 546.6 0 375 265 478 546.6 0 478 266 72 546.6 0 72 267 69 546.6 0 69 268 63 546.6 0 63 269 91 546.6 0 91 270 174 546.6 0 174 271 433 546.6 0 433 272 4263 546.6 0 4263 273 2198 546.6 0 2198 274 1997 546.6 0 1997 275 101 546.6 0 101 276 242 546.6 0 242 277 357 546.6 0 357 278 518 546.6 0 518 279 131 546.6 0 131 280 129 546.6 0 129 281 57 546.6 0 57 282 65 546.6 0 65 283 120 546.6 0 120 284 236 546.6 0 236 285 361 546.6 0 361 286 236 546.6 0 236 287 116 546.6 0 116 288 159 546.6 0 159 289 101 546.6 0 101 290 68 546.6 0 68 291 59 546.6 0 59 292 56 546.6 0 56 293 76 546.6 0 76 294 76 546.6 0 76 295 72 546.6 0 72 296 68 546.6 0 68 297 103 546.6 0 103 298 186 546.6 0 186 299 447 546.6 0 447 300 324 546.6 0 324 301 327 546.6 0 327 Finished in 2.13 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 45,855 Reads with adapters: 17,052 (37.2%) Reads that were too short: 54 (0.1%) Reads written (passing filters): 16,998 (37.1%) Total basepairs processed: 12,101,164 bp Total written (filtered): 4,690,643 bp (38.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17052 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 4 5 16 141 0.0 3 0 108 23 10 17 16533 0.0 3 15385 924 182 42 18 61 0.0 3 4 47 7 3 19 49 0.0 3 0 0 29 20 20 80 0.0 3 4 1 0 75 21 2 0.0 3 0 0 0 2 24 5 0.0 3 0 0 0 5 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 6 0.0 3 0 0 0 6 62 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 8 0.0 3 0 0 0 8 75 3 0.0 3 0 0 0 3 76 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 8 0.0 3 0 0 1 7 84 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 1 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 2 0.0 3 1 0 0 1 132 19 0.0 3 0 0 0 19 133 3 0.0 3 0 0 0 3 134 3 0.0 3 0 0 1 2 135 5 0.0 3 0 0 0 5 136 4 0.0 3 0 0 0 4 137 2 0.0 3 0 0 1 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 158 4 0.0 3 0 0 0 4 160 2 0.0 3 0 0 1 1 165 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 1 1 192 2 0.0 3 0 0 0 2 194 5 0.0 3 0 0 0 5 201 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 3 0.0 3 0 0 0 3 248 2 0.0 3 0 0 1 1 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 1 270 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.59 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 16,998 Reads with adapters: 214 (1.3%) Reads written (passing filters): 16,998 (100.0%) Total basepairs processed: 4,690,643 bp Total written (filtered): 4,689,991 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 214 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.8% C: 63.1% G: 14.0% T: 5.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 208 265.6 0 208 4 3 66.4 0 3 5 2 16.6 0 2 6 1 4.1 0 1