This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.71 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 121,649 (5.4%) Reads that were too short: 17,038 (0.8%) Reads written (passing filters): 104,611 (4.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 18,283,061 bp (2.7%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 121649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 150 546.6 0 150 7 109 546.6 0 109 8 117 546.6 0 117 9 190 546.6 0 190 10 77 546.6 0 77 11 117 546.6 0 117 12 96 546.6 0 96 13 89 546.6 0 89 14 86 546.6 0 86 15 108 546.6 0 108 16 90 546.6 0 90 17 107 546.6 0 107 18 89 546.6 0 89 19 86 546.6 0 86 20 92 546.6 0 92 21 130 546.6 0 130 22 152 546.6 0 152 23 156 546.6 0 156 24 165 546.6 0 165 25 326 546.6 0 326 26 758 546.6 0 758 27 94 546.6 0 94 28 100 546.6 0 100 29 78 546.6 0 78 30 102 546.6 0 102 31 74 546.6 0 74 32 86 546.6 0 86 33 88 546.6 0 88 34 81 546.6 0 81 35 92 546.6 0 92 36 95 546.6 0 95 37 89 546.6 0 89 38 83 546.6 0 83 39 98 546.6 0 98 40 100 546.6 0 100 41 91 546.6 0 91 42 73 546.6 0 73 43 94 546.6 0 94 44 89 546.6 0 89 45 101 546.6 0 101 46 95 546.6 0 95 47 92 546.6 0 92 48 93 546.6 0 93 49 108 546.6 0 108 50 92 546.6 0 92 51 113 546.6 0 113 52 72 546.6 0 72 53 99 546.6 0 99 54 117 546.6 0 117 55 122 546.6 0 122 56 115 546.6 0 115 57 278 546.6 0 278 58 138 546.6 0 138 59 110 546.6 0 110 60 89 546.6 0 89 61 170 546.6 0 170 62 406 546.6 0 406 63 294 546.6 0 294 64 103 546.6 0 103 65 98 546.6 0 98 66 107 546.6 0 107 67 104 546.6 0 104 68 205 546.6 0 205 69 132 546.6 0 132 70 175 546.6 0 175 71 164 546.6 0 164 72 82 546.6 0 82 73 102 546.6 0 102 74 103 546.6 0 103 75 94 546.6 0 94 76 85 546.6 0 85 77 104 546.6 0 104 78 294 546.6 0 294 79 112 546.6 0 112 80 192 546.6 0 192 81 145 546.6 0 145 82 213 546.6 0 213 83 346 546.6 0 346 84 827 546.6 0 827 85 970 546.6 0 970 86 88 546.6 0 88 87 111 546.6 0 111 88 142 546.6 0 142 89 112 546.6 0 112 90 114 546.6 0 114 91 73 546.6 0 73 92 89 546.6 0 89 93 95 546.6 0 95 94 98 546.6 0 98 95 88 546.6 0 88 96 112 546.6 0 112 97 113 546.6 0 113 98 150 546.6 0 150 99 208 546.6 0 208 100 121 546.6 0 121 101 169 546.6 0 169 102 186 546.6 0 186 103 126 546.6 0 126 104 112 546.6 0 112 105 158 546.6 0 158 106 118 546.6 0 118 107 236 546.6 0 236 108 176 546.6 0 176 109 182 546.6 0 182 110 276 546.6 0 276 111 262 546.6 0 262 112 3270 546.6 0 3270 113 4583 546.6 0 4583 114 17144 546.6 0 17144 115 185 546.6 0 185 116 114 546.6 0 114 117 128 546.6 0 128 118 262 546.6 0 262 119 180 546.6 0 180 120 1378 546.6 0 1378 121 1647 546.6 0 1647 122 3190 546.6 0 3190 123 309 546.6 0 309 124 356 546.6 0 356 125 1828 546.6 0 1828 126 18245 546.6 0 18245 127 7000 546.6 0 7000 128 143 546.6 0 143 129 126 546.6 0 126 130 93 546.6 0 93 131 136 546.6 0 136 132 102 546.6 0 102 133 107 546.6 0 107 134 117 546.6 0 117 135 94 546.6 0 94 136 88 546.6 0 88 137 115 546.6 0 115 138 158 546.6 0 158 139 115 546.6 0 115 140 122 546.6 0 122 141 121 546.6 0 121 142 155 546.6 0 155 143 219 546.6 0 219 144 262 546.6 0 262 145 124 546.6 0 124 146 133 546.6 0 133 147 390 546.6 0 390 148 536 546.6 0 536 149 1220 546.6 0 1220 150 518 546.6 0 518 151 390 546.6 0 390 152 396 546.6 0 396 153 127 546.6 0 127 154 128 546.6 0 128 155 103 546.6 0 103 156 107 546.6 0 107 157 142 546.6 0 142 158 138 546.6 0 138 159 130 546.6 0 130 160 149 546.6 0 149 161 145 546.6 0 145 162 115 546.6 0 115 163 150 546.6 0 150 164 138 546.6 0 138 165 274 546.6 0 274 166 322 546.6 0 322 167 467 546.6 0 467 168 544 546.6 0 544 169 482 546.6 0 482 170 497 546.6 0 497 171 1443 546.6 0 1443 172 750 546.6 0 750 173 1881 546.6 0 1881 174 335 546.6 0 335 175 317 546.6 0 317 176 240 546.6 0 240 177 137 546.6 0 137 178 109 546.6 0 109 179 110 546.6 0 110 180 127 546.6 0 127 181 149 546.6 0 149 182 238 546.6 0 238 183 1874 546.6 0 1874 184 359 546.6 0 359 185 1351 546.6 0 1351 186 166 546.6 0 166 187 255 546.6 0 255 188 268 546.6 0 268 189 201 546.6 0 201 190 138 546.6 0 138 191 1012 546.6 0 1012 192 976 546.6 0 976 193 2459 546.6 0 2459 194 892 546.6 0 892 195 157 546.6 0 157 196 288 546.6 0 288 197 192 546.6 0 192 198 1199 546.6 0 1199 199 314 546.6 0 314 200 346 546.6 0 346 201 298 546.6 0 298 202 139 546.6 0 139 203 98 546.6 0 98 204 91 546.6 0 91 205 109 546.6 0 109 206 103 546.6 0 103 207 142 546.6 0 142 208 101 546.6 0 101 209 131 546.6 0 131 210 113 546.6 0 113 211 90 546.6 0 90 212 90 546.6 0 90 213 116 546.6 0 116 214 105 546.6 0 105 215 211 546.6 0 211 216 147 546.6 0 147 217 147 546.6 0 147 218 154 546.6 0 154 219 135 546.6 0 135 220 104 546.6 0 104 221 122 546.6 0 122 222 91 546.6 0 91 223 112 546.6 0 112 224 136 546.6 0 136 225 154 546.6 0 154 226 174 546.6 0 174 227 265 546.6 0 265 228 414 546.6 0 414 229 225 546.6 0 225 230 423 546.6 0 423 231 114 546.6 0 114 232 99 546.6 0 99 233 88 546.6 0 88 234 84 546.6 0 84 235 119 546.6 0 119 236 83 546.6 0 83 237 124 546.6 0 124 238 106 546.6 0 106 239 108 546.6 0 108 240 99 546.6 0 99 241 98 546.6 0 98 242 106 546.6 0 106 243 237 546.6 0 237 244 156 546.6 0 156 245 154 546.6 0 154 246 148 546.6 0 148 247 181 546.6 0 181 248 129 546.6 0 129 249 77 546.6 0 77 250 80 546.6 0 80 251 121 546.6 0 121 252 144 546.6 0 144 253 153 546.6 0 153 254 93 546.6 0 93 255 136 546.6 0 136 256 139 546.6 0 139 257 181 546.6 0 181 258 77 546.6 0 77 259 93 546.6 0 93 260 100 546.6 0 100 261 111 546.6 0 111 262 124 546.6 0 124 263 135 546.6 0 135 264 124 546.6 0 124 265 140 546.6 0 140 266 117 546.6 0 117 267 705 546.6 0 705 268 1337 546.6 0 1337 269 1915 546.6 0 1915 270 107 546.6 0 107 271 106 546.6 0 106 272 97 546.6 0 97 273 76 546.6 0 76 274 83 546.6 0 83 275 100 546.6 0 100 276 121 546.6 0 121 277 170 546.6 0 170 278 103 546.6 0 103 279 143 546.6 0 143 280 117 546.6 0 117 281 157 546.6 0 157 282 175 546.6 0 175 283 99 546.6 0 99 284 149 546.6 0 149 285 71 546.6 0 71 286 107 546.6 0 107 287 77 546.6 0 77 288 92 546.6 0 92 289 88 546.6 0 88 290 113 546.6 0 113 291 104 546.6 0 104 292 80 546.6 0 80 293 106 546.6 0 106 294 101 546.6 0 101 295 137 546.6 0 137 296 113 546.6 0 113 297 80 546.6 0 80 298 271 546.6 0 271 299 665 546.6 0 665 300 154 546.6 0 154 301 99 546.6 0 99 Finished in 3.97 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 104,611 Reads with adapters: 113 (0.1%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 85 (0.1%) Total basepairs processed: 18,283,061 bp Total written (filtered): 16,831 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 113 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 20 21 0.0 4 20 1 22 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 41 1 0.0 4 0 0 0 0 1 49 4 0.0 4 0 0 0 0 4 51 45 0.0 4 0 0 0 0 45 60 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 10 0.0 4 0 0 0 0 10 113 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 1 1 140 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (118 us/read; 0.51 M reads/minute). === Summary === Total reads processed: 85 Reads with adapters: 7 (8.2%) Reads written (passing filters): 85 (100.0%) Total basepairs processed: 16,831 bp Total written (filtered): 16,503 bp (98.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 85.7% C: 0.0% G: 0.0% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 1.3 0 2 4 2 0.3 0 2 9 1 0.0 0 0 1 127 1 0.0 1 1 178 1 0.0 1 1