This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.81 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 67,747 (3.0%) Reads that were too short: 26,148 (1.2%) Reads written (passing filters): 41,599 (1.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 10,253,678 bp (1.5%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 67747 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 546.6 0 172 7 372 546.6 0 372 8 12626 546.6 0 12626 9 10278 546.6 0 10278 10 75 546.6 0 75 11 56 546.6 0 56 12 67 546.6 0 67 13 50 546.6 0 50 14 99 546.6 0 99 15 87 546.6 0 87 16 75 546.6 0 75 17 55 546.6 0 55 18 77 546.6 0 77 19 71 546.6 0 71 20 63 546.6 0 63 21 67 546.6 0 67 22 73 546.6 0 73 23 81 546.6 0 81 24 74 546.6 0 74 25 84 546.6 0 84 26 67 546.6 0 67 27 53 546.6 0 53 28 47 546.6 0 47 29 74 546.6 0 74 30 66 546.6 0 66 31 56 546.6 0 56 32 58 546.6 0 58 33 48 546.6 0 48 34 73 546.6 0 73 35 70 546.6 0 70 36 74 546.6 0 74 37 83 546.6 0 83 38 57 546.6 0 57 39 62 546.6 0 62 40 57 546.6 0 57 41 69 546.6 0 69 42 57 546.6 0 57 43 53 546.6 0 53 44 74 546.6 0 74 45 86 546.6 0 86 46 71 546.6 0 71 47 73 546.6 0 73 48 79 546.6 0 79 49 74 546.6 0 74 50 59 546.6 0 59 51 66 546.6 0 66 52 58 546.6 0 58 53 76 546.6 0 76 54 79 546.6 0 79 55 72 546.6 0 72 56 73 546.6 0 73 57 45 546.6 0 45 58 78 546.6 0 78 59 209 546.6 0 209 60 158 546.6 0 158 61 152 546.6 0 152 62 80 546.6 0 80 63 72 546.6 0 72 64 62 546.6 0 62 65 61 546.6 0 61 66 91 546.6 0 91 67 94 546.6 0 94 68 65 546.6 0 65 69 91 546.6 0 91 70 160 546.6 0 160 71 89 546.6 0 89 72 107 546.6 0 107 73 69 546.6 0 69 74 84 546.6 0 84 75 106 546.6 0 106 76 170 546.6 0 170 77 169 546.6 0 169 78 298 546.6 0 298 79 61 546.6 0 61 80 131 546.6 0 131 81 90 546.6 0 90 82 96 546.6 0 96 83 74 546.6 0 74 84 91 546.6 0 91 85 108 546.6 0 108 86 65 546.6 0 65 87 61 546.6 0 61 88 63 546.6 0 63 89 78 546.6 0 78 90 77 546.6 0 77 91 88 546.6 0 88 92 108 546.6 0 108 93 73 546.6 0 73 94 95 546.6 0 95 95 81 546.6 0 81 96 95 546.6 0 95 97 67 546.6 0 67 98 86 546.6 0 86 99 73 546.6 0 73 100 75 546.6 0 75 101 72 546.6 0 72 102 81 546.6 0 81 103 109 546.6 0 109 104 56 546.6 0 56 105 78 546.6 0 78 106 77 546.6 0 77 107 83 546.6 0 83 108 77 546.6 0 77 109 85 546.6 0 85 110 77 546.6 0 77 111 98 546.6 0 98 112 135 546.6 0 135 113 95 546.6 0 95 114 97 546.6 0 97 115 94 546.6 0 94 116 69 546.6 0 69 117 91 546.6 0 91 118 86 546.6 0 86 119 83 546.6 0 83 120 72 546.6 0 72 121 77 546.6 0 77 122 62 546.6 0 62 123 64 546.6 0 64 124 89 546.6 0 89 125 75 546.6 0 75 126 91 546.6 0 91 127 76 546.6 0 76 128 75 546.6 0 75 129 104 546.6 0 104 130 157 546.6 0 157 131 140 546.6 0 140 132 170 546.6 0 170 133 159 546.6 0 159 134 163 546.6 0 163 135 116 546.6 0 116 136 91 546.6 0 91 137 75 546.6 0 75 138 223 546.6 0 223 139 187 546.6 0 187 140 118 546.6 0 118 141 108 546.6 0 108 142 87 546.6 0 87 143 124 546.6 0 124 144 149 546.6 0 149 145 120 546.6 0 120 146 154 546.6 0 154 147 101 546.6 0 101 148 82 546.6 0 82 149 78 546.6 0 78 150 64 546.6 0 64 151 108 546.6 0 108 152 115 546.6 0 115 153 105 546.6 0 105 154 82 546.6 0 82 155 105 546.6 0 105 156 110 546.6 0 110 157 83 546.6 0 83 158 340 546.6 0 340 159 106 546.6 0 106 160 73 546.6 0 73 161 71 546.6 0 71 162 104 546.6 0 104 163 65 546.6 0 65 164 82 546.6 0 82 165 90 546.6 0 90 166 66 546.6 0 66 167 62 546.6 0 62 168 79 546.6 0 79 169 74 546.6 0 74 170 66 546.6 0 66 171 76 546.6 0 76 172 80 546.6 0 80 173 85 546.6 0 85 174 78 546.6 0 78 175 70 546.6 0 70 176 86 546.6 0 86 177 89 546.6 0 89 178 75 546.6 0 75 179 63 546.6 0 63 180 89 546.6 0 89 181 89 546.6 0 89 182 81 546.6 0 81 183 96 546.6 0 96 184 66 546.6 0 66 185 63 546.6 0 63 186 75 546.6 0 75 187 71 546.6 0 71 188 84 546.6 0 84 189 76 546.6 0 76 190 106 546.6 0 106 191 202 546.6 0 202 192 222 546.6 0 222 193 146 546.6 0 146 194 285 546.6 0 285 195 77 546.6 0 77 196 87 546.6 0 87 197 285 546.6 0 285 198 192 546.6 0 192 199 272 546.6 0 272 200 68 546.6 0 68 201 73 546.6 0 73 202 88 546.6 0 88 203 70 546.6 0 70 204 144 546.6 0 144 205 92 546.6 0 92 206 166 546.6 0 166 207 66 546.6 0 66 208 56 546.6 0 56 209 66 546.6 0 66 210 63 546.6 0 63 211 102 546.6 0 102 212 86 546.6 0 86 213 66 546.6 0 66 214 91 546.6 0 91 215 72 546.6 0 72 216 72 546.6 0 72 217 67 546.6 0 67 218 89 546.6 0 89 219 65 546.6 0 65 220 78 546.6 0 78 221 91 546.6 0 91 222 89 546.6 0 89 223 211 546.6 0 211 224 135 546.6 0 135 225 363 546.6 0 363 226 66 546.6 0 66 227 92 546.6 0 92 228 76 546.6 0 76 229 73 546.6 0 73 230 55 546.6 0 55 231 78 546.6 0 78 232 92 546.6 0 92 233 128 546.6 0 128 234 177 546.6 0 177 235 149 546.6 0 149 236 99 546.6 0 99 237 185 546.6 0 185 238 203 546.6 0 203 239 141 546.6 0 141 240 136 546.6 0 136 241 120 546.6 0 120 242 131 546.6 0 131 243 150 546.6 0 150 244 202 546.6 0 202 245 124 546.6 0 124 246 120 546.6 0 120 247 163 546.6 0 163 248 117 546.6 0 117 249 104 546.6 0 104 250 97 546.6 0 97 251 78 546.6 0 78 252 88 546.6 0 88 253 63 546.6 0 63 254 151 546.6 0 151 255 167 546.6 0 167 256 246 546.6 0 246 257 180 546.6 0 180 258 308 546.6 0 308 259 186 546.6 0 186 260 170 546.6 0 170 261 197 546.6 0 197 262 183 546.6 0 183 263 185 546.6 0 185 264 177 546.6 0 177 265 1049 546.6 0 1049 266 891 546.6 0 891 267 2077 546.6 0 2077 268 344 546.6 0 344 269 57 546.6 0 57 270 98 546.6 0 98 271 141 546.6 0 141 272 197 546.6 0 197 273 237 546.6 0 237 274 225 546.6 0 225 275 182 546.6 0 182 276 108 546.6 0 108 277 115 546.6 0 115 278 111 546.6 0 111 279 142 546.6 0 142 280 157 546.6 0 157 281 158 546.6 0 158 282 121 546.6 0 121 283 145 546.6 0 145 284 118 546.6 0 118 285 148 546.6 0 148 286 159 546.6 0 159 287 154 546.6 0 154 288 203 546.6 0 203 289 197 546.6 0 197 290 221 546.6 0 221 291 289 546.6 0 289 292 242 546.6 0 242 293 286 546.6 0 286 294 342 546.6 0 342 295 298 546.6 0 298 296 260 546.6 0 260 297 459 546.6 0 459 298 1151 546.6 0 1151 299 1826 546.6 0 1826 300 3740 546.6 0 3740 301 1555 546.6 0 1555 Finished in 1.82 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 41,599 Reads with adapters: 12,349 (29.7%) Reads that were too short: 55 (0.1%) Reads written (passing filters): 12,294 (29.6%) Total basepairs processed: 10,253,678 bp Total written (filtered): 3,388,031 bp (33.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12349 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 0 2 16 94 0.0 3 0 77 14 3 17 12037 0.0 3 11164 703 145 25 18 55 0.0 3 3 28 1 23 19 46 0.0 3 0 0 26 20 20 23 0.0 3 3 0 0 20 24 1 0.0 3 1 27 2 0.0 3 1 0 0 1 39 3 0.0 3 0 0 0 3 40 3 0.0 3 0 0 1 2 43 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 1 1 48 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 115 8 0.0 3 0 0 0 8 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 128 4 0.0 3 0 0 1 3 137 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 1 189 19 0.0 3 0 0 0 19 192 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 208 1 0.0 3 0 0 0 1 209 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 12,294 Reads with adapters: 66 (0.5%) Reads written (passing filters): 12,294 (100.0%) Total basepairs processed: 3,388,031 bp Total written (filtered): 3,387,804 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 66 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.2% C: 10.6% G: 60.6% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 43 192.1 0 43 4 19 48.0 0 19 5 3 12.0 0 3 7 1 0.8 0 1