This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.41 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 68,035 (3.0%) Reads that were too short: 29,365 (1.3%) Reads written (passing filters): 38,670 (1.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,763,873 bp (1.4%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 68035 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 138 546.6 0 138 7 320 546.6 0 320 8 11674 546.6 0 11674 9 11980 546.6 0 11980 10 52 546.6 0 52 11 58 546.6 0 58 12 64 546.6 0 64 13 65 546.6 0 65 14 67 546.6 0 67 15 72 546.6 0 72 16 55 546.6 0 55 17 54 546.6 0 54 18 63 546.6 0 63 19 55 546.6 0 55 20 63 546.6 0 63 21 58 546.6 0 58 22 65 546.6 0 65 23 70 546.6 0 70 24 53 546.6 0 53 25 57 546.6 0 57 26 58 546.6 0 58 27 45 546.6 0 45 28 37 546.6 0 37 29 67 546.6 0 67 30 37 546.6 0 37 31 45 546.6 0 45 32 45 546.6 0 45 33 37 546.6 0 37 34 59 546.6 0 59 35 41 546.6 0 41 36 41 546.6 0 41 37 57 546.6 0 57 38 35 546.6 0 35 39 41 546.6 0 41 40 58 546.6 0 58 41 51 546.6 0 51 42 53 546.6 0 53 43 41 546.6 0 41 44 56 546.6 0 56 45 79 546.6 0 79 46 69 546.6 0 69 47 61 546.6 0 61 48 66 546.6 0 66 49 60 546.6 0 60 50 38 546.6 0 38 51 34 546.6 0 34 52 51 546.6 0 51 53 49 546.6 0 49 54 52 546.6 0 52 55 44 546.6 0 44 56 81 546.6 0 81 57 50 546.6 0 50 58 69 546.6 0 69 59 261 546.6 0 261 60 161 546.6 0 161 61 167 546.6 0 167 62 37 546.6 0 37 63 52 546.6 0 52 64 59 546.6 0 59 65 62 546.6 0 62 66 59 546.6 0 59 67 70 546.6 0 70 68 58 546.6 0 58 69 58 546.6 0 58 70 171 546.6 0 171 71 68 546.6 0 68 72 119 546.6 0 119 73 63 546.6 0 63 74 65 546.6 0 65 75 82 546.6 0 82 76 87 546.6 0 87 77 90 546.6 0 90 78 168 546.6 0 168 79 66 546.6 0 66 80 82 546.6 0 82 81 88 546.6 0 88 82 70 546.6 0 70 83 54 546.6 0 54 84 55 546.6 0 55 85 69 546.6 0 69 86 47 546.6 0 47 87 55 546.6 0 55 88 53 546.6 0 53 89 61 546.6 0 61 90 51 546.6 0 51 91 74 546.6 0 74 92 85 546.6 0 85 93 75 546.6 0 75 94 75 546.6 0 75 95 87 546.6 0 87 96 55 546.6 0 55 97 51 546.6 0 51 98 72 546.6 0 72 99 63 546.6 0 63 100 68 546.6 0 68 101 74 546.6 0 74 102 52 546.6 0 52 103 66 546.6 0 66 104 55 546.6 0 55 105 60 546.6 0 60 106 69 546.6 0 69 107 50 546.6 0 50 108 60 546.6 0 60 109 61 546.6 0 61 110 73 546.6 0 73 111 70 546.6 0 70 112 79 546.6 0 79 113 63 546.6 0 63 114 56 546.6 0 56 115 55 546.6 0 55 116 53 546.6 0 53 117 84 546.6 0 84 118 72 546.6 0 72 119 48 546.6 0 48 120 65 546.6 0 65 121 54 546.6 0 54 122 66 546.6 0 66 123 54 546.6 0 54 124 61 546.6 0 61 125 55 546.6 0 55 126 51 546.6 0 51 127 60 546.6 0 60 128 65 546.6 0 65 129 79 546.6 0 79 130 110 546.6 0 110 131 115 546.6 0 115 132 114 546.6 0 114 133 130 546.6 0 130 134 132 546.6 0 132 135 73 546.6 0 73 136 67 546.6 0 67 137 67 546.6 0 67 138 216 546.6 0 216 139 166 546.6 0 166 140 132 546.6 0 132 141 76 546.6 0 76 142 59 546.6 0 59 143 91 546.6 0 91 144 146 546.6 0 146 145 72 546.6 0 72 146 111 546.6 0 111 147 81 546.6 0 81 148 48 546.6 0 48 149 64 546.6 0 64 150 57 546.6 0 57 151 54 546.6 0 54 152 72 546.6 0 72 153 62 546.6 0 62 154 66 546.6 0 66 155 77 546.6 0 77 156 68 546.6 0 68 157 81 546.6 0 81 158 343 546.6 0 343 159 97 546.6 0 97 160 82 546.6 0 82 161 43 546.6 0 43 162 56 546.6 0 56 163 46 546.6 0 46 164 48 546.6 0 48 165 84 546.6 0 84 166 49 546.6 0 49 167 43 546.6 0 43 168 48 546.6 0 48 169 51 546.6 0 51 170 54 546.6 0 54 171 50 546.6 0 50 172 53 546.6 0 53 173 47 546.6 0 47 174 58 546.6 0 58 175 50 546.6 0 50 176 42 546.6 0 42 177 55 546.6 0 55 178 53 546.6 0 53 179 46 546.6 0 46 180 57 546.6 0 57 181 70 546.6 0 70 182 51 546.6 0 51 183 45 546.6 0 45 184 43 546.6 0 43 185 49 546.6 0 49 186 65 546.6 0 65 187 47 546.6 0 47 188 56 546.6 0 56 189 74 546.6 0 74 190 69 546.6 0 69 191 186 546.6 0 186 192 287 546.6 0 287 193 177 546.6 0 177 194 274 546.6 0 274 195 59 546.6 0 59 196 72 546.6 0 72 197 314 546.6 0 314 198 153 546.6 0 153 199 292 546.6 0 292 200 51 546.6 0 51 201 61 546.6 0 61 202 47 546.6 0 47 203 44 546.6 0 44 204 206 546.6 0 206 205 56 546.6 0 56 206 191 546.6 0 191 207 59 546.6 0 59 208 46 546.6 0 46 209 62 546.6 0 62 210 44 546.6 0 44 211 71 546.6 0 71 212 54 546.6 0 54 213 71 546.6 0 71 214 80 546.6 0 80 215 72 546.6 0 72 216 53 546.6 0 53 217 51 546.6 0 51 218 75 546.6 0 75 219 55 546.6 0 55 220 58 546.6 0 58 221 57 546.6 0 57 222 96 546.6 0 96 223 300 546.6 0 300 224 156 546.6 0 156 225 519 546.6 0 519 226 75 546.6 0 75 227 71 546.6 0 71 228 37 546.6 0 37 229 56 546.6 0 56 230 45 546.6 0 45 231 84 546.6 0 84 232 78 546.6 0 78 233 112 546.6 0 112 234 98 546.6 0 98 235 88 546.6 0 88 236 85 546.6 0 85 237 120 546.6 0 120 238 126 546.6 0 126 239 84 546.6 0 84 240 91 546.6 0 91 241 91 546.6 0 91 242 74 546.6 0 74 243 65 546.6 0 65 244 130 546.6 0 130 245 70 546.6 0 70 246 93 546.6 0 93 247 98 546.6 0 98 248 55 546.6 0 55 249 58 546.6 0 58 250 55 546.6 0 55 251 66 546.6 0 66 252 62 546.6 0 62 253 69 546.6 0 69 254 102 546.6 0 102 255 139 546.6 0 139 256 169 546.6 0 169 257 130 546.6 0 130 258 191 546.6 0 191 259 138 546.6 0 138 260 114 546.6 0 114 261 152 546.6 0 152 262 93 546.6 0 93 263 119 546.6 0 119 264 87 546.6 0 87 265 298 546.6 0 298 266 295 546.6 0 295 267 644 546.6 0 644 268 160 546.6 0 160 269 46 546.6 0 46 270 48 546.6 0 48 271 77 546.6 0 77 272 115 546.6 0 115 273 151 546.6 0 151 274 128 546.6 0 128 275 104 546.6 0 104 276 77 546.6 0 77 277 94 546.6 0 94 278 119 546.6 0 119 279 112 546.6 0 112 280 172 546.6 0 172 281 145 546.6 0 145 282 146 546.6 0 146 283 146 546.6 0 146 284 145 546.6 0 145 285 161 546.6 0 161 286 149 546.6 0 149 287 152 546.6 0 152 288 241 546.6 0 241 289 273 546.6 0 273 290 280 546.6 0 280 291 512 546.6 0 512 292 458 546.6 0 458 293 417 546.6 0 417 294 507 546.6 0 507 295 449 546.6 0 449 296 420 546.6 0 420 297 701 546.6 0 701 298 1956 546.6 0 1956 299 3081 546.6 0 3081 300 6326 546.6 0 6326 301 3867 546.6 0 3867 Finished in 1.94 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 38,670 Reads with adapters: 14,042 (36.3%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 14,031 (36.3%) Total basepairs processed: 9,763,873 bp Total written (filtered): 3,661,798 bp (37.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14042 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 202 0.0 3 0 195 6 0 1 20 12311 0.0 4 12047 253 11 21 28 0.0 4 5 23 22 3 0.0 4 1 0 2 23 12 0.0 4 1 0 0 10 1 46 1 0.0 4 1 93 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 10 0.0 4 0 0 0 0 10 127 1448 0.0 4 0 0 0 0 1448 128 6 0.0 4 0 0 0 0 6 137 1 0.0 4 0 0 0 0 1 183 1 0.0 4 1 185 2 0.0 4 1 0 1 186 3 0.0 4 3 187 1 0.0 4 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 14,031 Reads with adapters: 2,037 (14.5%) Reads written (passing filters): 14,031 (100.0%) Total basepairs processed: 3,661,798 bp Total written (filtered): 3,642,485 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2037 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 2.4% G: 95.2% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 219.2 0 4 4 4 54.8 0 4 5 1 13.7 0 1 9 1145 0.1 0 23 1122 10 756 0.0 1 38 718 11 115 0.0 1 1 114 12 6 0.0 1 0 6 13 6 0.0 1 0 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.