This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.34 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 48,455 (2.2%) Reads that were too short: 27,164 (1.2%) Reads written (passing filters): 21,291 (1.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 4,010,282 bp (0.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 48455 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 144 546.6 0 144 7 615 546.6 0 615 8 642 546.6 0 642 9 46 546.6 0 46 10 38 546.6 0 38 11 54 546.6 0 54 12 61 546.6 0 61 13 45 546.6 0 45 14 56 546.6 0 56 15 33 546.6 0 33 16 48 546.6 0 48 17 46 546.6 0 46 18 52 546.6 0 52 19 49 546.6 0 49 20 30 546.6 0 30 21 42 546.6 0 42 22 48 546.6 0 48 23 54 546.6 0 54 24 47 546.6 0 47 25 46 546.6 0 46 26 39 546.6 0 39 27 38 546.6 0 38 28 43 546.6 0 43 29 76 546.6 0 76 30 60 546.6 0 60 31 36 546.6 0 36 32 47 546.6 0 47 33 30 546.6 0 30 34 26 546.6 0 26 35 45 546.6 0 45 36 48 546.6 0 48 37 51 546.6 0 51 38 45 546.6 0 45 39 47 546.6 0 47 40 55 546.6 0 55 41 101 546.6 0 101 42 69 546.6 0 69 43 79 546.6 0 79 44 57 546.6 0 57 45 38 546.6 0 38 46 56 546.6 0 56 47 52 546.6 0 52 48 44 546.6 0 44 49 51 546.6 0 51 50 60 546.6 0 60 51 51 546.6 0 51 52 66 546.6 0 66 53 92 546.6 0 92 54 158 546.6 0 158 55 54 546.6 0 54 56 92 546.6 0 92 57 42 546.6 0 42 58 47 546.6 0 47 59 60 546.6 0 60 60 50 546.6 0 50 61 45 546.6 0 45 62 73 546.6 0 73 63 120 546.6 0 120 64 1420 546.6 0 1420 65 274 546.6 0 274 66 464 546.6 0 464 67 100 546.6 0 100 68 68 546.6 0 68 69 77 546.6 0 77 70 93 546.6 0 93 71 51 546.6 0 51 72 41 546.6 0 41 73 44 546.6 0 44 74 43 546.6 0 43 75 65 546.6 0 65 76 47 546.6 0 47 77 55 546.6 0 55 78 40 546.6 0 40 79 48 546.6 0 48 80 45 546.6 0 45 81 68 546.6 0 68 82 32 546.6 0 32 83 65 546.6 0 65 84 67 546.6 0 67 85 55 546.6 0 55 86 58 546.6 0 58 87 38 546.6 0 38 88 54 546.6 0 54 89 97 546.6 0 97 90 97 546.6 0 97 91 93 546.6 0 93 92 479 546.6 0 479 93 221 546.6 0 221 94 135 546.6 0 135 95 52 546.6 0 52 96 30 546.6 0 30 97 46 546.6 0 46 98 27 546.6 0 27 99 75 546.6 0 75 100 82 546.6 0 82 101 218 546.6 0 218 102 57 546.6 0 57 103 45 546.6 0 45 104 49 546.6 0 49 105 52 546.6 0 52 106 38 546.6 0 38 107 34 546.6 0 34 108 55 546.6 0 55 109 53 546.6 0 53 110 52 546.6 0 52 111 72 546.6 0 72 112 49 546.6 0 49 113 41 546.6 0 41 114 71 546.6 0 71 115 52 546.6 0 52 116 39 546.6 0 39 117 72 546.6 0 72 118 70 546.6 0 70 119 63 546.6 0 63 120 60 546.6 0 60 121 62 546.6 0 62 122 63 546.6 0 63 123 83 546.6 0 83 124 59 546.6 0 59 125 68 546.6 0 68 126 60 546.6 0 60 127 62 546.6 0 62 128 71 546.6 0 71 129 99 546.6 0 99 130 137 546.6 0 137 131 193 546.6 0 193 132 146 546.6 0 146 133 87 546.6 0 87 134 79 546.6 0 79 135 185 546.6 0 185 136 195 546.6 0 195 137 262 546.6 0 262 138 71 546.6 0 71 139 91 546.6 0 91 140 69 546.6 0 69 141 55 546.6 0 55 142 78 546.6 0 78 143 62 546.6 0 62 144 71 546.6 0 71 145 69 546.6 0 69 146 61 546.6 0 61 147 162 546.6 0 162 148 91 546.6 0 91 149 121 546.6 0 121 150 89 546.6 0 89 151 78 546.6 0 78 152 110 546.6 0 110 153 101 546.6 0 101 154 85 546.6 0 85 155 135 546.6 0 135 156 188 546.6 0 188 157 379 546.6 0 379 158 320 546.6 0 320 159 108 546.6 0 108 160 104 546.6 0 104 161 78 546.6 0 78 162 101 546.6 0 101 163 109 546.6 0 109 164 80 546.6 0 80 165 65 546.6 0 65 166 140 546.6 0 140 167 357 546.6 0 357 168 214 546.6 0 214 169 245 546.6 0 245 170 101 546.6 0 101 171 174 546.6 0 174 172 211 546.6 0 211 173 322 546.6 0 322 174 86 546.6 0 86 175 340 546.6 0 340 176 242 546.6 0 242 177 321 546.6 0 321 178 153 546.6 0 153 179 75 546.6 0 75 180 82 546.6 0 82 181 80 546.6 0 80 182 93 546.6 0 93 183 143 546.6 0 143 184 93 546.6 0 93 185 94 546.6 0 94 186 154 546.6 0 154 187 144 546.6 0 144 188 419 546.6 0 419 189 179 546.6 0 179 190 122 546.6 0 122 191 78 546.6 0 78 192 74 546.6 0 74 193 94 546.6 0 94 194 80 546.6 0 80 195 97 546.6 0 97 196 158 546.6 0 158 197 108 546.6 0 108 198 100 546.6 0 100 199 98 546.6 0 98 200 135 546.6 0 135 201 116 546.6 0 116 202 125 546.6 0 125 203 149 546.6 0 149 204 130 546.6 0 130 205 77 546.6 0 77 206 77 546.6 0 77 207 70 546.6 0 70 208 91 546.6 0 91 209 79 546.6 0 79 210 164 546.6 0 164 211 112 546.6 0 112 212 107 546.6 0 107 213 99 546.6 0 99 214 140 546.6 0 140 215 192 546.6 0 192 216 171 546.6 0 171 217 212 546.6 0 212 218 240 546.6 0 240 219 215 546.6 0 215 220 265 546.6 0 265 221 417 546.6 0 417 222 669 546.6 0 669 223 1090 546.6 0 1090 224 583 546.6 0 583 225 364 546.6 0 364 226 336 546.6 0 336 227 164 546.6 0 164 228 169 546.6 0 169 229 101 546.6 0 101 230 97 546.6 0 97 231 100 546.6 0 100 232 94 546.6 0 94 233 90 546.6 0 90 234 137 546.6 0 137 235 106 546.6 0 106 236 115 546.6 0 115 237 162 546.6 0 162 238 119 546.6 0 119 239 152 546.6 0 152 240 161 546.6 0 161 241 704 546.6 0 704 242 532 546.6 0 532 243 1253 546.6 0 1253 244 584 546.6 0 584 245 311 546.6 0 311 246 196 546.6 0 196 247 137 546.6 0 137 248 135 546.6 0 135 249 341 546.6 0 341 250 198 546.6 0 198 251 166 546.6 0 166 252 243 546.6 0 243 253 564 546.6 0 564 254 1125 546.6 0 1125 255 187 546.6 0 187 256 194 546.6 0 194 257 145 546.6 0 145 258 105 546.6 0 105 259 68 546.6 0 68 260 91 546.6 0 91 261 148 546.6 0 148 262 198 546.6 0 198 263 227 546.6 0 227 264 241 546.6 0 241 265 219 546.6 0 219 266 304 546.6 0 304 267 111 546.6 0 111 268 261 546.6 0 261 269 414 546.6 0 414 270 1304 546.6 0 1304 271 451 546.6 0 451 272 559 546.6 0 559 273 356 546.6 0 356 274 301 546.6 0 301 275 262 546.6 0 262 276 138 546.6 0 138 277 161 546.6 0 161 278 181 546.6 0 181 279 131 546.6 0 131 280 135 546.6 0 135 281 141 546.6 0 141 282 231 546.6 0 231 283 232 546.6 0 232 284 275 546.6 0 275 285 189 546.6 0 189 286 191 546.6 0 191 287 237 546.6 0 237 288 153 546.6 0 153 289 265 546.6 0 265 290 206 546.6 0 206 291 500 546.6 0 500 292 740 546.6 0 740 293 453 546.6 0 453 294 195 546.6 0 195 295 113 546.6 0 113 296 162 546.6 0 162 297 173 546.6 0 173 298 281 546.6 0 281 299 452 546.6 0 452 300 299 546.6 0 299 301 354 546.6 0 354 Finished in 0.76 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 21,291 Reads with adapters: 837 (3.9%) Reads that were too short: 84 (0.4%) Reads written (passing filters): 753 (3.5%) Total basepairs processed: 4,010,282 bp Total written (filtered): 195,601 bp (4.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 837 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 3 0.0 3 0 1 0 2 17 590 0.0 3 550 30 7 3 18 1 0.0 3 0 1 19 4 0.0 3 2 2 20 93 0.0 3 68 4 2 19 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 1 23 5 0.0 3 0 0 1 4 24 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 36 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 41 6 0.0 3 0 0 0 6 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 15 0.0 3 0 0 0 15 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 1 0 1 64 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 160 21 0.0 3 0 0 1 20 161 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 753 Reads with adapters: 5 (0.7%) Reads written (passing filters): 753 (100.0%) Total basepairs processed: 195,601 bp Total written (filtered): 195,586 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 40.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 11.8 0 5