This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 55.88 s (25 us/read; 2.40 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 260,855 (11.7%) Reads that were too short: 225,470 (10.1%) Reads written (passing filters): 35,385 (1.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 8,008,601 bp (1.2%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 260855 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 207 546.6 0 207 7 7347 546.6 0 7347 8 261 546.6 0 261 9 6742 546.6 0 6742 10 82 546.6 0 82 11 73 546.6 0 73 12 54 546.6 0 54 13 60 546.6 0 60 14 78 546.6 0 78 15 76 546.6 0 76 16 50 546.6 0 50 17 54 546.6 0 54 18 61 546.6 0 61 19 56 546.6 0 56 20 67 546.6 0 67 21 51 546.6 0 51 22 44 546.6 0 44 23 58 546.6 0 58 24 61 546.6 0 61 25 68 546.6 0 68 26 61 546.6 0 61 27 88 546.6 0 88 28 78 546.6 0 78 29 79 546.6 0 79 30 90 546.6 0 90 31 83 546.6 0 83 32 74 546.6 0 74 33 70 546.6 0 70 34 59 546.6 0 59 35 84 546.6 0 84 36 154 546.6 0 154 37 206 546.6 0 206 38 195 546.6 0 195 39 54 546.6 0 54 40 63 546.6 0 63 41 47 546.6 0 47 42 38 546.6 0 38 43 59 546.6 0 59 44 54 546.6 0 54 45 54 546.6 0 54 46 52 546.6 0 52 47 61 546.6 0 61 48 40 546.6 0 40 49 46 546.6 0 46 50 43 546.6 0 43 51 29 546.6 0 29 52 43 546.6 0 43 53 49 546.6 0 49 54 50 546.6 0 50 55 26 546.6 0 26 56 299 546.6 0 299 57 88 546.6 0 88 58 41 546.6 0 41 59 45 546.6 0 45 60 53 546.6 0 53 61 46 546.6 0 46 62 43 546.6 0 43 63 29 546.6 0 29 64 44 546.6 0 44 65 49 546.6 0 49 66 52 546.6 0 52 67 66 546.6 0 66 68 48 546.6 0 48 69 84 546.6 0 84 70 69 546.6 0 69 71 148 546.6 0 148 72 61 546.6 0 61 73 96 546.6 0 96 74 59 546.6 0 59 75 89 546.6 0 89 76 87 546.6 0 87 77 91 546.6 0 91 78 88 546.6 0 88 79 67 546.6 0 67 80 60 546.6 0 60 81 82 546.6 0 82 82 81 546.6 0 81 83 96 546.6 0 96 84 69 546.6 0 69 85 79 546.6 0 79 86 78 546.6 0 78 87 62 546.6 0 62 88 89 546.6 0 89 89 47 546.6 0 47 90 53 546.6 0 53 91 48 546.6 0 48 92 55 546.6 0 55 93 50 546.6 0 50 94 65 546.6 0 65 95 65 546.6 0 65 96 140 546.6 0 140 97 82 546.6 0 82 98 66 546.6 0 66 99 103 546.6 0 103 100 73 546.6 0 73 101 66 546.6 0 66 102 70 546.6 0 70 103 73 546.6 0 73 104 78 546.6 0 78 105 62 546.6 0 62 106 78 546.6 0 78 107 91 546.6 0 91 108 81 546.6 0 81 109 77 546.6 0 77 110 96 546.6 0 96 111 95 546.6 0 95 112 112 546.6 0 112 113 77 546.6 0 77 114 79 546.6 0 79 115 104 546.6 0 104 116 80 546.6 0 80 117 58 546.6 0 58 118 508 546.6 0 508 119 83 546.6 0 83 120 132 546.6 0 132 121 92 546.6 0 92 122 121 546.6 0 121 123 157 546.6 0 157 124 202 546.6 0 202 125 116 546.6 0 116 126 99 546.6 0 99 127 75 546.6 0 75 128 94 546.6 0 94 129 64 546.6 0 64 130 62 546.6 0 62 131 114 546.6 0 114 132 81 546.6 0 81 133 109 546.6 0 109 134 92 546.6 0 92 135 78 546.6 0 78 136 85 546.6 0 85 137 78 546.6 0 78 138 133 546.6 0 133 139 221 546.6 0 221 140 205 546.6 0 205 141 157 546.6 0 157 142 75 546.6 0 75 143 74 546.6 0 74 144 70 546.6 0 70 145 91 546.6 0 91 146 69 546.6 0 69 147 90 546.6 0 90 148 100 546.6 0 100 149 54 546.6 0 54 150 55 546.6 0 55 151 37 546.6 0 37 152 39 546.6 0 39 153 62 546.6 0 62 154 76 546.6 0 76 155 101 546.6 0 101 156 96 546.6 0 96 157 119 546.6 0 119 158 240 546.6 0 240 159 2067 546.6 0 2067 160 1281 546.6 0 1281 161 2396 546.6 0 2396 162 85 546.6 0 85 163 68 546.6 0 68 164 45 546.6 0 45 165 72 546.6 0 72 166 69 546.6 0 69 167 48 546.6 0 48 168 66 546.6 0 66 169 53 546.6 0 53 170 60 546.6 0 60 171 45 546.6 0 45 172 58 546.6 0 58 173 75 546.6 0 75 174 74 546.6 0 74 175 117 546.6 0 117 176 84 546.6 0 84 177 59 546.6 0 59 178 40 546.6 0 40 179 67 546.6 0 67 180 59 546.6 0 59 181 95 546.6 0 95 182 60 546.6 0 60 183 48 546.6 0 48 184 59 546.6 0 59 185 73 546.6 0 73 186 51 546.6 0 51 187 76 546.6 0 76 188 48 546.6 0 48 189 55 546.6 0 55 190 48 546.6 0 48 191 43 546.6 0 43 192 66 546.6 0 66 193 77 546.6 0 77 194 70 546.6 0 70 195 59 546.6 0 59 196 52 546.6 0 52 197 63 546.6 0 63 198 100 546.6 0 100 199 90 546.6 0 90 200 66 546.6 0 66 201 51 546.6 0 51 202 70 546.6 0 70 203 68 546.6 0 68 204 62 546.6 0 62 205 90 546.6 0 90 206 121 546.6 0 121 207 217 546.6 0 217 208 172 546.6 0 172 209 354 546.6 0 354 210 194 546.6 0 194 211 240 546.6 0 240 212 215 546.6 0 215 213 285 546.6 0 285 214 229 546.6 0 229 215 69 546.6 0 69 216 80 546.6 0 80 217 101 546.6 0 101 218 302 546.6 0 302 219 145 546.6 0 145 220 95 546.6 0 95 221 142 546.6 0 142 222 121 546.6 0 121 223 114 546.6 0 114 224 119 546.6 0 119 225 224 546.6 0 224 226 85 546.6 0 85 227 174 546.6 0 174 228 437 546.6 0 437 229 202 546.6 0 202 230 110 546.6 0 110 231 127 546.6 0 127 232 1011 546.6 0 1011 233 341 546.6 0 341 234 1310 546.6 0 1310 235 208 546.6 0 208 236 146 546.6 0 146 237 256 546.6 0 256 238 628 546.6 0 628 239 164 546.6 0 164 240 266 546.6 0 266 241 168 546.6 0 168 242 204 546.6 0 204 243 260 546.6 0 260 244 149 546.6 0 149 245 118 546.6 0 118 246 79 546.6 0 79 247 118 546.6 0 118 248 271 546.6 0 271 249 299 546.6 0 299 250 576 546.6 0 576 251 923 546.6 0 923 252 1298 546.6 0 1298 253 2874 546.6 0 2874 254 11279 546.6 0 11279 255 99457 546.6 0 99457 256 56877 546.6 0 56877 257 36740 546.6 0 36740 258 1480 546.6 0 1480 259 98 546.6 0 98 260 189 546.6 0 189 261 121 546.6 0 121 262 128 546.6 0 128 263 82 546.6 0 82 264 78 546.6 0 78 265 62 546.6 0 62 266 115 546.6 0 115 267 243 546.6 0 243 268 172 546.6 0 172 269 35 546.6 0 35 270 33 546.6 0 33 271 48 546.6 0 48 272 39 546.6 0 39 273 59 546.6 0 59 274 55 546.6 0 55 275 53 546.6 0 53 276 48 546.6 0 48 277 39 546.6 0 39 278 34 546.6 0 34 279 63 546.6 0 63 280 61 546.6 0 61 281 49 546.6 0 49 282 52 546.6 0 52 283 51 546.6 0 51 284 72 546.6 0 72 285 32 546.6 0 32 286 37 546.6 0 37 287 36 546.6 0 36 288 44 546.6 0 44 289 29 546.6 0 29 290 49 546.6 0 49 291 46 546.6 0 46 292 44 546.6 0 44 293 43 546.6 0 43 294 54 546.6 0 54 295 65 546.6 0 65 296 49 546.6 0 49 297 48 546.6 0 48 298 45 546.6 0 45 299 74 546.6 0 74 300 114 546.6 0 114 301 118 546.6 0 118 Finished in 1.46 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 35,385 Reads with adapters: 6,938 (19.6%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 6,896 (19.5%) Total basepairs processed: 8,008,601 bp Total written (filtered): 1,893,404 bp (23.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6938 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 91 0.0 3 0 74 10 7 17 6701 0.0 3 6257 363 65 16 18 36 0.0 3 1 12 2 21 19 29 0.0 3 0 1 16 12 20 15 0.0 3 1 0 0 14 22 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 111 3 0.0 3 1 1 0 1 113 1 0.0 3 0 0 0 1 115 9 0.0 3 0 0 0 9 137 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 6,896 Reads with adapters: 498 (7.2%) Reads written (passing filters): 6,896 (100.0%) Total basepairs processed: 1,893,404 bp Total written (filtered): 1,891,901 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 498 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.2% C: 15.5% G: 21.3% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 491 107.8 0 491 4 5 26.9 0 5 5 2 6.7 0 2