This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.15 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 98,310 (4.4%) Reads that were too short: 30,485 (1.4%) Reads written (passing filters): 67,825 (3.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,041,218 bp (1.3%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 98310 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 26 546.6 0 26 7 31 546.6 0 31 8 95 546.6 0 95 9 129 546.6 0 129 10 24 546.6 0 24 11 34 546.6 0 34 12 22 546.6 0 22 13 31 546.6 0 31 14 30 546.6 0 30 15 15 546.6 0 15 16 30 546.6 0 30 17 32 546.6 0 32 18 25 546.6 0 25 19 28 546.6 0 28 20 31 546.6 0 31 21 69 546.6 0 69 22 28 546.6 0 28 23 51 546.6 0 51 24 29 546.6 0 29 25 18 546.6 0 18 26 65 546.6 0 65 27 115 546.6 0 115 28 18 546.6 0 18 29 43 546.6 0 43 30 22 546.6 0 22 31 62 546.6 0 62 32 28 546.6 0 28 33 40 546.6 0 40 34 39 546.6 0 39 35 31 546.6 0 31 36 30 546.6 0 30 37 45 546.6 0 45 38 29 546.6 0 29 39 22 546.6 0 22 40 17 546.6 0 17 41 27 546.6 0 27 42 26 546.6 0 26 43 18 546.6 0 18 44 26 546.6 0 26 45 27 546.6 0 27 46 26 546.6 0 26 47 37 546.6 0 37 48 26 546.6 0 26 49 26 546.6 0 26 50 33 546.6 0 33 51 40 546.6 0 40 52 26 546.6 0 26 53 44 546.6 0 44 54 27 546.6 0 27 55 24 546.6 0 24 56 29 546.6 0 29 57 27 546.6 0 27 58 19 546.6 0 19 59 28 546.6 0 28 60 33 546.6 0 33 61 33 546.6 0 33 62 18 546.6 0 18 63 32 546.6 0 32 64 45 546.6 0 45 65 34 546.6 0 34 66 56 546.6 0 56 67 41 546.6 0 41 68 76 546.6 0 76 69 363 546.6 0 363 70 67 546.6 0 67 71 201 546.6 0 201 72 21 546.6 0 21 73 29 546.6 0 29 74 26 546.6 0 26 75 46 546.6 0 46 76 48 546.6 0 48 77 58 546.6 0 58 78 61 546.6 0 61 79 203 546.6 0 203 80 108 546.6 0 108 81 459 546.6 0 459 82 66 546.6 0 66 83 39 546.6 0 39 84 51 546.6 0 51 85 28 546.6 0 28 86 32 546.6 0 32 87 47 546.6 0 47 88 35 546.6 0 35 89 22 546.6 0 22 90 32 546.6 0 32 91 34 546.6 0 34 92 31 546.6 0 31 93 24 546.6 0 24 94 38 546.6 0 38 95 33 546.6 0 33 96 32 546.6 0 32 97 25 546.6 0 25 98 38 546.6 0 38 99 93 546.6 0 93 100 74 546.6 0 74 101 116 546.6 0 116 102 62 546.6 0 62 103 34 546.6 0 34 104 25 546.6 0 25 105 34 546.6 0 34 106 23 546.6 0 23 107 37 546.6 0 37 108 25 546.6 0 25 109 33 546.6 0 33 110 38 546.6 0 38 111 48 546.6 0 48 112 82 546.6 0 82 113 112 546.6 0 112 114 128 546.6 0 128 115 81 546.6 0 81 116 56 546.6 0 56 117 389 546.6 0 389 118 234 546.6 0 234 119 258 546.6 0 258 120 67 546.6 0 67 121 63 546.6 0 63 122 55 546.6 0 55 123 31 546.6 0 31 124 37 546.6 0 37 125 37 546.6 0 37 126 63 546.6 0 63 127 72 546.6 0 72 128 96 546.6 0 96 129 64 546.6 0 64 130 98 546.6 0 98 131 692 546.6 0 692 132 323 546.6 0 323 133 784 546.6 0 784 134 89 546.6 0 89 135 74 546.6 0 74 136 75 546.6 0 75 137 77 546.6 0 77 138 118 546.6 0 118 139 123 546.6 0 123 140 112 546.6 0 112 141 36 546.6 0 36 142 31 546.6 0 31 143 52 546.6 0 52 144 66 546.6 0 66 145 65 546.6 0 65 146 80 546.6 0 80 147 92 546.6 0 92 148 54 546.6 0 54 149 52 546.6 0 52 150 52 546.6 0 52 151 36 546.6 0 36 152 49 546.6 0 49 153 48 546.6 0 48 154 82 546.6 0 82 155 50 546.6 0 50 156 97 546.6 0 97 157 129 546.6 0 129 158 169 546.6 0 169 159 48 546.6 0 48 160 97 546.6 0 97 161 92 546.6 0 92 162 73 546.6 0 73 163 79 546.6 0 79 164 103 546.6 0 103 165 215 546.6 0 215 166 90 546.6 0 90 167 145 546.6 0 145 168 182 546.6 0 182 169 131 546.6 0 131 170 163 546.6 0 163 171 192 546.6 0 192 172 161 546.6 0 161 173 243 546.6 0 243 174 358 546.6 0 358 175 3698 546.6 0 3698 176 534 546.6 0 534 177 756 546.6 0 756 178 1554 546.6 0 1554 179 7851 546.6 0 7851 180 22627 546.6 0 22627 181 2717 546.6 0 2717 182 7335 546.6 0 7335 183 1345 546.6 0 1345 184 346 546.6 0 346 185 392 546.6 0 392 186 272 546.6 0 272 187 50 546.6 0 50 188 120 546.6 0 120 189 56 546.6 0 56 190 91 546.6 0 91 191 123 546.6 0 123 192 56 546.6 0 56 193 80 546.6 0 80 194 132 546.6 0 132 195 297 546.6 0 297 196 1062 546.6 0 1062 197 1148 546.6 0 1148 198 635 546.6 0 635 199 224 546.6 0 224 200 261 546.6 0 261 201 734 546.6 0 734 202 1196 546.6 0 1196 203 1696 546.6 0 1696 204 713 546.6 0 713 205 31 546.6 0 31 206 42 546.6 0 42 207 33 546.6 0 33 208 52 546.6 0 52 209 57 546.6 0 57 210 51 546.6 0 51 211 36 546.6 0 36 212 34 546.6 0 34 213 48 546.6 0 48 214 48 546.6 0 48 215 225 546.6 0 225 216 108 546.6 0 108 217 274 546.6 0 274 218 55 546.6 0 55 219 67 546.6 0 67 220 72 546.6 0 72 221 67 546.6 0 67 222 240 546.6 0 240 223 316 546.6 0 316 224 453 546.6 0 453 225 247 546.6 0 247 226 68 546.6 0 68 227 42 546.6 0 42 228 41 546.6 0 41 229 39 546.6 0 39 230 48 546.6 0 48 231 61 546.6 0 61 232 62 546.6 0 62 233 64 546.6 0 64 234 374 546.6 0 374 235 170 546.6 0 170 236 415 546.6 0 415 237 64 546.6 0 64 238 71 546.6 0 71 239 320 546.6 0 320 240 763 546.6 0 763 241 735 546.6 0 735 242 294 546.6 0 294 243 370 546.6 0 370 244 382 546.6 0 382 245 221 546.6 0 221 246 76 546.6 0 76 247 58 546.6 0 58 248 41 546.6 0 41 249 55 546.6 0 55 250 67 546.6 0 67 251 71 546.6 0 71 252 144 546.6 0 144 253 118 546.6 0 118 254 126 546.6 0 126 255 156 546.6 0 156 256 154 546.6 0 154 257 344 546.6 0 344 258 350 546.6 0 350 259 1386 546.6 0 1386 260 1018 546.6 0 1018 261 2077 546.6 0 2077 262 1049 546.6 0 1049 263 248 546.6 0 248 264 676 546.6 0 676 265 1561 546.6 0 1561 266 2473 546.6 0 2473 267 1732 546.6 0 1732 268 38 546.6 0 38 269 44 546.6 0 44 270 69 546.6 0 69 271 59 546.6 0 59 272 61 546.6 0 61 273 74 546.6 0 74 274 84 546.6 0 84 275 145 546.6 0 145 276 207 546.6 0 207 277 244 546.6 0 244 278 321 546.6 0 321 279 340 546.6 0 340 280 56 546.6 0 56 281 34 546.6 0 34 282 40 546.6 0 40 283 46 546.6 0 46 284 40 546.6 0 40 285 72 546.6 0 72 286 57 546.6 0 57 287 59 546.6 0 59 288 46 546.6 0 46 289 59 546.6 0 59 290 103 546.6 0 103 291 571 546.6 0 571 292 632 546.6 0 632 293 1068 546.6 0 1068 294 202 546.6 0 202 295 154 546.6 0 154 296 157 546.6 0 157 297 99 546.6 0 99 298 101 546.6 0 101 299 147 546.6 0 147 300 142 546.6 0 142 301 169 546.6 0 169 Finished in 1.95 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 67,825 Reads with adapters: 372 (0.5%) Reads that were too short: 154 (0.2%) Reads written (passing filters): 218 (0.3%) Total basepairs processed: 9,041,218 bp Total written (filtered): 32,274 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 372 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 7 0.0 3 0 0 0 7 17 24 0.0 3 16 6 1 1 18 9 0.0 3 2 2 3 2 19 5 0.0 3 3 0 1 1 20 9 0.0 3 7 2 27 6 0.0 3 0 0 0 6 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 30 4 0.0 3 0 0 0 4 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 7 0.0 3 0 0 0 7 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 41 6 0.0 3 0 0 0 6 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 50 5 0.0 3 0 0 0 5 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 68 0.0 3 0 0 2 66 61 6 0.0 3 0 0 0 6 62 4 0.0 3 0 0 0 4 63 17 0.0 3 0 0 2 15 64 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 14 0.0 3 0 0 0 14 75 2 0.0 3 0 0 0 2 77 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 79 4 0.0 3 0 0 0 4 80 7 0.0 3 0 0 0 7 83 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 9 0.0 3 0 0 0 9 92 5 0.0 3 0 0 0 5 93 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 17 0.0 3 0 0 0 17 103 4 0.0 3 0 0 0 4 104 3 0.0 3 0 0 0 3 105 15 0.0 3 0 0 0 15 106 1 0.0 3 0 0 0 1 110 4 0.0 3 0 0 0 4 111 5 0.0 3 0 0 0 5 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 159 5 0.0 3 0 0 0 5 160 2 0.0 3 0 0 0 2 161 5 0.0 3 0 0 0 5 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 218 Reads with adapters: 0 (0.0%) Reads written (passing filters): 218 (100.0%) Total basepairs processed: 32,274 bp Total written (filtered): 32,274 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.