This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.65 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 77,511 (3.5%) Reads that were too short: 17,748 (0.8%) Reads written (passing filters): 59,763 (2.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 7,806,225 bp (1.2%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 77511 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 20 546.6 0 20 7 30 546.6 0 30 8 91 546.6 0 91 9 111 546.6 0 111 10 21 546.6 0 21 11 19 546.6 0 19 12 22 546.6 0 22 13 21 546.6 0 21 14 13 546.6 0 13 15 18 546.6 0 18 16 16 546.6 0 16 17 20 546.6 0 20 18 17 546.6 0 17 19 19 546.6 0 19 20 16 546.6 0 16 21 31 546.6 0 31 22 20 546.6 0 20 23 26 546.6 0 26 24 16 546.6 0 16 25 13 546.6 0 13 26 31 546.6 0 31 27 15 546.6 0 15 28 14 546.6 0 14 29 31 546.6 0 31 30 15 546.6 0 15 31 49 546.6 0 49 32 20 546.6 0 20 33 33 546.6 0 33 34 30 546.6 0 30 35 23 546.6 0 23 36 18 546.6 0 18 37 30 546.6 0 30 38 36 546.6 0 36 39 24 546.6 0 24 40 21 546.6 0 21 41 12 546.6 0 12 42 21 546.6 0 21 43 20 546.6 0 20 44 22 546.6 0 22 45 16 546.6 0 16 46 15 546.6 0 15 47 20 546.6 0 20 48 17 546.6 0 17 49 17 546.6 0 17 50 16 546.6 0 16 51 23 546.6 0 23 52 15 546.6 0 15 53 27 546.6 0 27 54 29 546.6 0 29 55 24 546.6 0 24 56 17 546.6 0 17 57 21 546.6 0 21 58 22 546.6 0 22 59 27 546.6 0 27 60 15 546.6 0 15 61 16 546.6 0 16 62 19 546.6 0 19 63 28 546.6 0 28 64 39 546.6 0 39 65 28 546.6 0 28 66 28 546.6 0 28 67 32 546.6 0 32 68 50 546.6 0 50 69 436 546.6 0 436 70 58 546.6 0 58 71 243 546.6 0 243 72 19 546.6 0 19 73 18 546.6 0 18 74 28 546.6 0 28 75 19 546.6 0 19 76 32 546.6 0 32 77 38 546.6 0 38 78 51 546.6 0 51 79 150 546.6 0 150 80 42 546.6 0 42 81 533 546.6 0 533 82 22 546.6 0 22 83 18 546.6 0 18 84 21 546.6 0 21 85 19 546.6 0 19 86 11 546.6 0 11 87 13 546.6 0 13 88 18 546.6 0 18 89 16 546.6 0 16 90 15 546.6 0 15 91 20 546.6 0 20 92 7 546.6 0 7 93 18 546.6 0 18 94 21 546.6 0 21 95 23 546.6 0 23 96 30 546.6 0 30 97 20 546.6 0 20 98 33 546.6 0 33 99 58 546.6 0 58 100 29 546.6 0 29 101 30 546.6 0 30 102 19 546.6 0 19 103 22 546.6 0 22 104 15 546.6 0 15 105 22 546.6 0 22 106 26 546.6 0 26 107 24 546.6 0 24 108 16 546.6 0 16 109 18 546.6 0 18 110 28 546.6 0 28 111 23 546.6 0 23 112 29 546.6 0 29 113 59 546.6 0 59 114 43 546.6 0 43 115 21 546.6 0 21 116 25 546.6 0 25 117 162 546.6 0 162 118 96 546.6 0 96 119 94 546.6 0 94 120 36 546.6 0 36 121 29 546.6 0 29 122 31 546.6 0 31 123 26 546.6 0 26 124 22 546.6 0 22 125 38 546.6 0 38 126 29 546.6 0 29 127 47 546.6 0 47 128 69 546.6 0 69 129 41 546.6 0 41 130 33 546.6 0 33 131 235 546.6 0 235 132 158 546.6 0 158 133 229 546.6 0 229 134 45 546.6 0 45 135 40 546.6 0 40 136 43 546.6 0 43 137 40 546.6 0 40 138 83 546.6 0 83 139 62 546.6 0 62 140 45 546.6 0 45 141 31 546.6 0 31 142 18 546.6 0 18 143 30 546.6 0 30 144 31 546.6 0 31 145 31 546.6 0 31 146 40 546.6 0 40 147 59 546.6 0 59 148 41 546.6 0 41 149 29 546.6 0 29 150 30 546.6 0 30 151 31 546.6 0 31 152 43 546.6 0 43 153 35 546.6 0 35 154 66 546.6 0 66 155 39 546.6 0 39 156 111 546.6 0 111 157 84 546.6 0 84 158 104 546.6 0 104 159 32 546.6 0 32 160 68 546.6 0 68 161 62 546.6 0 62 162 53 546.6 0 53 163 71 546.6 0 71 164 105 546.6 0 105 165 207 546.6 0 207 166 71 546.6 0 71 167 131 546.6 0 131 168 126 546.6 0 126 169 108 546.6 0 108 170 126 546.6 0 126 171 166 546.6 0 166 172 143 546.6 0 143 173 202 546.6 0 202 174 291 546.6 0 291 175 3713 546.6 0 3713 176 378 546.6 0 378 177 700 546.6 0 700 178 1248 546.6 0 1248 179 7572 546.6 0 7572 180 22790 546.6 0 22790 181 2859 546.6 0 2859 182 8342 546.6 0 8342 183 854 546.6 0 854 184 105 546.6 0 105 185 94 546.6 0 94 186 57 546.6 0 57 187 36 546.6 0 36 188 81 546.6 0 81 189 32 546.6 0 32 190 37 546.6 0 37 191 97 546.6 0 97 192 39 546.6 0 39 193 47 546.6 0 47 194 60 546.6 0 60 195 147 546.6 0 147 196 480 546.6 0 480 197 512 546.6 0 512 198 316 546.6 0 316 199 99 546.6 0 99 200 223 546.6 0 223 201 212 546.6 0 212 202 558 546.6 0 558 203 597 546.6 0 597 204 232 546.6 0 232 205 24 546.6 0 24 206 35 546.6 0 35 207 27 546.6 0 27 208 17 546.6 0 17 209 22 546.6 0 22 210 21 546.6 0 21 211 20 546.6 0 20 212 29 546.6 0 29 213 27 546.6 0 27 214 22 546.6 0 22 215 114 546.6 0 114 216 59 546.6 0 59 217 174 546.6 0 174 218 28 546.6 0 28 219 28 546.6 0 28 220 30 546.6 0 30 221 42 546.6 0 42 222 86 546.6 0 86 223 131 546.6 0 131 224 201 546.6 0 201 225 152 546.6 0 152 226 34 546.6 0 34 227 35 546.6 0 35 228 21 546.6 0 21 229 30 546.6 0 30 230 30 546.6 0 30 231 40 546.6 0 40 232 32 546.6 0 32 233 49 546.6 0 49 234 462 546.6 0 462 235 170 546.6 0 170 236 458 546.6 0 458 237 44 546.6 0 44 238 30 546.6 0 30 239 238 546.6 0 238 240 580 546.6 0 580 241 565 546.6 0 565 242 108 546.6 0 108 243 137 546.6 0 137 244 123 546.6 0 123 245 137 546.6 0 137 246 45 546.6 0 45 247 25 546.6 0 25 248 26 546.6 0 26 249 38 546.6 0 38 250 33 546.6 0 33 251 43 546.6 0 43 252 75 546.6 0 75 253 65 546.6 0 65 254 66 546.6 0 66 255 99 546.6 0 99 256 166 546.6 0 166 257 191 546.6 0 191 258 159 546.6 0 159 259 860 546.6 0 860 260 802 546.6 0 802 261 1267 546.6 0 1267 262 549 546.6 0 549 263 154 546.6 0 154 264 536 546.6 0 536 265 868 546.6 0 868 266 1340 546.6 0 1340 267 889 546.6 0 889 268 25 546.6 0 25 269 22 546.6 0 22 270 35 546.6 0 35 271 23 546.6 0 23 272 39 546.6 0 39 273 37 546.6 0 37 274 40 546.6 0 40 275 43 546.6 0 43 276 69 546.6 0 69 277 60 546.6 0 60 278 96 546.6 0 96 279 126 546.6 0 126 280 33 546.6 0 33 281 29 546.6 0 29 282 17 546.6 0 17 283 37 546.6 0 37 284 25 546.6 0 25 285 25 546.6 0 25 286 36 546.6 0 36 287 51 546.6 0 51 288 36 546.6 0 36 289 39 546.6 0 39 290 55 546.6 0 55 291 417 546.6 0 417 292 464 546.6 0 464 293 756 546.6 0 756 294 147 546.6 0 147 295 114 546.6 0 114 296 101 546.6 0 101 297 119 546.6 0 119 298 60 546.6 0 60 299 71 546.6 0 71 300 91 546.6 0 91 301 115 546.6 0 115 Finished in 1.89 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 59,763 Reads with adapters: 111 (0.2%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 105 (0.2%) Total basepairs processed: 7,806,225 bp Total written (filtered): 28,348 bp (0.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 111 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 2 0.0 3 0 2 20 95 0.0 4 94 1 21 3 0.0 4 1 1 1 22 2 0.0 4 0 0 0 2 27 1 0.0 4 0 0 0 1 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (95 us/read; 0.63 M reads/minute). === Summary === Total reads processed: 105 Reads with adapters: 6 (5.7%) Reads written (passing filters): 105 (100.0%) Total basepairs processed: 28,348 bp Total written (filtered): 28,290 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 4 0.0 1 0 4