This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.13 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 124,050 (5.5%) Reads that were too short: 81,646 (3.6%) Reads written (passing filters): 42,404 (1.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,486,753 bp (1.4%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 124050 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 287 546.6 0 287 7 4247 546.6 0 4247 8 3411 546.6 0 3411 9 14 546.6 0 14 10 23 546.6 0 23 11 12 546.6 0 12 12 27 546.6 0 27 13 19 546.6 0 19 14 13 546.6 0 13 15 25 546.6 0 25 16 12 546.6 0 12 17 18 546.6 0 18 18 19 546.6 0 19 19 22 546.6 0 22 20 27 546.6 0 27 21 39 546.6 0 39 22 14 546.6 0 14 23 7 546.6 0 7 24 27 546.6 0 27 25 18 546.6 0 18 26 11 546.6 0 11 27 14 546.6 0 14 28 16 546.6 0 16 29 40 546.6 0 40 30 84 546.6 0 84 31 24 546.6 0 24 32 15 546.6 0 15 33 17 546.6 0 17 34 11 546.6 0 11 35 18 546.6 0 18 36 7 546.6 0 7 37 20 546.6 0 20 38 19 546.6 0 19 39 18 546.6 0 18 40 30 546.6 0 30 41 17 546.6 0 17 42 20 546.6 0 20 43 14 546.6 0 14 44 14 546.6 0 14 45 14 546.6 0 14 46 25 546.6 0 25 47 38 546.6 0 38 48 32 546.6 0 32 49 45 546.6 0 45 50 72 546.6 0 72 51 64 546.6 0 64 52 37 546.6 0 37 53 21 546.6 0 21 54 34 546.6 0 34 55 43 546.6 0 43 56 46 546.6 0 46 57 47 546.6 0 47 58 45 546.6 0 45 59 46 546.6 0 46 60 78 546.6 0 78 61 93 546.6 0 93 62 179 546.6 0 179 63 76 546.6 0 76 64 85 546.6 0 85 65 151 546.6 0 151 66 53 546.6 0 53 67 50 546.6 0 50 68 121 546.6 0 121 69 111 546.6 0 111 70 164 546.6 0 164 71 174 546.6 0 174 72 200 546.6 0 200 73 388 546.6 0 388 74 476 546.6 0 476 75 5674 546.6 0 5674 76 5938 546.6 0 5938 77 11355 546.6 0 11355 78 59 546.6 0 59 79 31 546.6 0 31 80 22 546.6 0 22 81 19 546.6 0 19 82 22 546.6 0 22 83 19 546.6 0 19 84 26 546.6 0 26 85 9 546.6 0 9 86 21 546.6 0 21 87 12 546.6 0 12 88 26 546.6 0 26 89 15 546.6 0 15 90 35 546.6 0 35 91 23 546.6 0 23 92 32 546.6 0 32 93 11 546.6 0 11 94 21 546.6 0 21 95 27 546.6 0 27 96 11 546.6 0 11 97 12 546.6 0 12 98 17 546.6 0 17 99 8 546.6 0 8 100 9 546.6 0 9 101 11 546.6 0 11 102 10 546.6 0 10 103 13 546.6 0 13 104 7 546.6 0 7 105 7 546.6 0 7 106 15 546.6 0 15 107 11 546.6 0 11 108 11 546.6 0 11 109 11 546.6 0 11 110 19 546.6 0 19 111 16 546.6 0 16 112 18 546.6 0 18 113 15 546.6 0 15 114 18 546.6 0 18 115 30 546.6 0 30 116 26 546.6 0 26 117 31 546.6 0 31 118 22 546.6 0 22 119 22 546.6 0 22 120 39 546.6 0 39 121 205 546.6 0 205 122 23 546.6 0 23 123 29 546.6 0 29 124 17 546.6 0 17 125 36 546.6 0 36 126 90 546.6 0 90 127 738 546.6 0 738 128 55 546.6 0 55 129 45 546.6 0 45 130 57 546.6 0 57 131 52 546.6 0 52 132 28 546.6 0 28 133 34 546.6 0 34 134 35 546.6 0 35 135 110 546.6 0 110 136 110 546.6 0 110 137 180 546.6 0 180 138 28 546.6 0 28 139 21 546.6 0 21 140 19 546.6 0 19 141 16 546.6 0 16 142 44 546.6 0 44 143 49 546.6 0 49 144 55 546.6 0 55 145 51 546.6 0 51 146 10 546.6 0 10 147 15 546.6 0 15 148 20 546.6 0 20 149 22 546.6 0 22 150 22 546.6 0 22 151 14 546.6 0 14 152 14 546.6 0 14 153 24 546.6 0 24 154 18 546.6 0 18 155 16 546.6 0 16 156 25 546.6 0 25 157 40 546.6 0 40 158 22 546.6 0 22 159 14 546.6 0 14 160 12 546.6 0 12 161 23 546.6 0 23 162 30 546.6 0 30 163 59 546.6 0 59 164 43 546.6 0 43 165 37 546.6 0 37 166 56 546.6 0 56 167 73 546.6 0 73 168 152 546.6 0 152 169 89 546.6 0 89 170 79 546.6 0 79 171 42 546.6 0 42 172 15 546.6 0 15 173 18 546.6 0 18 174 15 546.6 0 15 175 27 546.6 0 27 176 32 546.6 0 32 177 22 546.6 0 22 178 23 546.6 0 23 179 37 546.6 0 37 180 62 546.6 0 62 181 45 546.6 0 45 182 64 546.6 0 64 183 53 546.6 0 53 184 59 546.6 0 59 185 51 546.6 0 51 186 216 546.6 0 216 187 155 546.6 0 155 188 346 546.6 0 346 189 156 546.6 0 156 190 58 546.6 0 58 191 45 546.6 0 45 192 77 546.6 0 77 193 182 546.6 0 182 194 204 546.6 0 204 195 296 546.6 0 296 196 145 546.6 0 145 197 310 546.6 0 310 198 303 546.6 0 303 199 406 546.6 0 406 200 132 546.6 0 132 201 248 546.6 0 248 202 176 546.6 0 176 203 466 546.6 0 466 204 343 546.6 0 343 205 281 546.6 0 281 206 58 546.6 0 58 207 33 546.6 0 33 208 46 546.6 0 46 209 64 546.6 0 64 210 78 546.6 0 78 211 74 546.6 0 74 212 43 546.6 0 43 213 82 546.6 0 82 214 140 546.6 0 140 215 194 546.6 0 194 216 122 546.6 0 122 217 118 546.6 0 118 218 185 546.6 0 185 219 294 546.6 0 294 220 213 546.6 0 213 221 75 546.6 0 75 222 108 546.6 0 108 223 231 546.6 0 231 224 433 546.6 0 433 225 278 546.6 0 278 226 166 546.6 0 166 227 91 546.6 0 91 228 66 546.6 0 66 229 132 546.6 0 132 230 114 546.6 0 114 231 99 546.6 0 99 232 96 546.6 0 96 233 156 546.6 0 156 234 241 546.6 0 241 235 342 546.6 0 342 236 435 546.6 0 435 237 815 546.6 0 815 238 7158 546.6 0 7158 239 2209 546.6 0 2209 240 7472 546.6 0 7472 241 268 546.6 0 268 242 160 546.6 0 160 243 195 546.6 0 195 244 282 546.6 0 282 245 261 546.6 0 261 246 516 546.6 0 516 247 580 546.6 0 580 248 4038 546.6 0 4038 249 18643 546.6 0 18643 250 13967 546.6 0 13967 251 6150 546.6 0 6150 252 185 546.6 0 185 253 182 546.6 0 182 254 181 546.6 0 181 255 98 546.6 0 98 256 108 546.6 0 108 257 123 546.6 0 123 258 50 546.6 0 50 259 118 546.6 0 118 260 51 546.6 0 51 261 79 546.6 0 79 262 65 546.6 0 65 263 167 546.6 0 167 264 67 546.6 0 67 265 60 546.6 0 60 266 73 546.6 0 73 267 55 546.6 0 55 268 79 546.6 0 79 269 107 546.6 0 107 270 116 546.6 0 116 271 150 546.6 0 150 272 69 546.6 0 69 273 89 546.6 0 89 274 66 546.6 0 66 275 77 546.6 0 77 276 67 546.6 0 67 277 95 546.6 0 95 278 143 546.6 0 143 279 195 546.6 0 195 280 285 546.6 0 285 281 271 546.6 0 271 282 538 546.6 0 538 283 863 546.6 0 863 284 2266 546.6 0 2266 285 963 546.6 0 963 286 255 546.6 0 255 287 263 546.6 0 263 288 277 546.6 0 277 289 147 546.6 0 147 290 117 546.6 0 117 291 176 546.6 0 176 292 173 546.6 0 173 293 166 546.6 0 166 294 218 546.6 0 218 295 387 546.6 0 387 296 690 546.6 0 690 297 570 546.6 0 570 298 403 546.6 0 403 299 307 546.6 0 307 300 278 546.6 0 278 301 331 546.6 0 331 Finished in 1.73 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 42,404 Reads with adapters: 5,233 (12.3%) Reads that were too short: 211 (0.5%) Reads written (passing filters): 5,022 (11.8%) Total basepairs processed: 9,486,753 bp Total written (filtered): 1,239,144 bp (13.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 42 0.0 3 0 32 7 3 17 3979 0.0 3 3691 241 39 8 18 13 0.0 3 3 7 1 2 19 11 0.0 3 2 0 6 3 20 7 0.0 3 0 0 0 7 21 2 0.0 3 0 0 0 2 23 2 0.0 3 0 1 0 1 24 1 0.0 3 0 0 0 1 25 4 0.0 3 0 0 0 4 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 47 12 0.0 3 0 0 1 11 48 3 0.0 3 0 0 0 3 51 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 6 0.0 3 0 0 0 6 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 22 0.0 3 0 0 0 22 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 12 0.0 3 0 0 0 12 76 2 0.0 3 0 0 1 1 77 4 0.0 3 0 0 0 4 78 3 0.0 3 0 0 1 2 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 6 0.0 3 0 0 0 6 87 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 1 3 90 2 0.0 3 0 0 0 2 91 50 0.0 3 0 0 0 50 92 21 0.0 3 0 0 0 21 93 11 0.0 3 0 0 0 11 94 9 0.0 3 0 0 1 8 95 3 0.0 3 0 0 0 3 96 114 0.0 3 0 0 4 110 97 3 0.0 3 0 0 0 3 98 49 0.0 3 0 0 2 47 99 42 0.0 3 0 0 0 42 100 19 0.0 3 0 0 2 17 101 7 0.0 3 0 0 0 7 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 5 0.0 3 0 0 0 5 105 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 107 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 109 69 0.0 3 0 0 2 67 110 1 0.0 3 0 0 0 1 111 7 0.0 3 0 0 0 7 112 7 0.0 3 0 0 1 6 113 4 0.0 3 0 0 0 4 114 3 0.0 3 0 0 0 3 115 14 0.0 3 0 0 0 14 116 296 0.0 3 0 0 2 294 117 45 0.0 3 0 0 1 44 118 11 0.0 3 0 0 0 11 119 46 0.0 3 0 0 3 43 120 13 0.0 3 0 0 0 13 121 1 0.0 3 0 0 0 1 122 12 0.0 3 0 0 0 12 123 1 0.0 3 0 0 0 1 124 9 0.0 3 0 0 1 8 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 142 9 0.0 3 0 0 0 9 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 167 7 0.0 3 0 0 0 7 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 7 0.0 3 0 0 0 7 177 2 0.0 3 0 0 0 2 178 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 6 0.0 3 0 0 0 6 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 203 7 0.0 3 0 0 0 7 205 1 0.0 3 0 0 0 1 206 4 0.0 3 0 0 0 4 207 10 0.0 3 0 0 0 10 208 4 0.0 3 0 0 0 4 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 0 4 217 21 0.0 3 0 0 2 19 218 4 0.0 3 0 0 0 4 219 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 226 6 0.0 3 0 0 0 6 228 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 5,022 Reads with adapters: 97 (1.9%) Reads written (passing filters): 5,022 (100.0%) Total basepairs processed: 1,239,144 bp Total written (filtered): 1,238,850 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 97 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.5% C: 71.1% G: 11.3% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 96 78.5 0 96 6 1 1.2 0 1