This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.97 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 123,060 (5.5%) Reads that were too short: 92,825 (4.1%) Reads written (passing filters): 30,235 (1.4%) Total basepairs processed: 673,916,425 bp Total written (filtered): 7,087,107 bp (1.1%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 123060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 303 546.6 0 303 7 3924 546.6 0 3924 8 3991 546.6 0 3991 9 19 546.6 0 19 10 17 546.6 0 17 11 15 546.6 0 15 12 13 546.6 0 13 13 20 546.6 0 20 14 19 546.6 0 19 15 21 546.6 0 21 16 10 546.6 0 10 17 13 546.6 0 13 18 16 546.6 0 16 19 15 546.6 0 15 20 15 546.6 0 15 21 26 546.6 0 26 22 16 546.6 0 16 23 13 546.6 0 13 24 17 546.6 0 17 25 13 546.6 0 13 26 11 546.6 0 11 27 11 546.6 0 11 28 16 546.6 0 16 29 16 546.6 0 16 30 17 546.6 0 17 31 12 546.6 0 12 32 10 546.6 0 10 33 11 546.6 0 11 34 12 546.6 0 12 35 20 546.6 0 20 36 10 546.6 0 10 37 6 546.6 0 6 38 13 546.6 0 13 39 19 546.6 0 19 40 22 546.6 0 22 41 11 546.6 0 11 42 23 546.6 0 23 43 19 546.6 0 19 44 13 546.6 0 13 45 8 546.6 0 8 46 10 546.6 0 10 47 11 546.6 0 11 48 25 546.6 0 25 49 21 546.6 0 21 50 27 546.6 0 27 51 27 546.6 0 27 52 19 546.6 0 19 53 24 546.6 0 24 54 31 546.6 0 31 55 32 546.6 0 32 56 44 546.6 0 44 57 41 546.6 0 41 58 26 546.6 0 26 59 33 546.6 0 33 60 45 546.6 0 45 61 62 546.6 0 62 62 156 546.6 0 156 63 60 546.6 0 60 64 59 546.6 0 59 65 122 546.6 0 122 66 36 546.6 0 36 67 37 546.6 0 37 68 75 546.6 0 75 69 63 546.6 0 63 70 78 546.6 0 78 71 86 546.6 0 86 72 91 546.6 0 91 73 153 546.6 0 153 74 160 546.6 0 160 75 2975 546.6 0 2975 76 3591 546.6 0 3591 77 8997 546.6 0 8997 78 37 546.6 0 37 79 15 546.6 0 15 80 15 546.6 0 15 81 17 546.6 0 17 82 16 546.6 0 16 83 29 546.6 0 29 84 11 546.6 0 11 85 11 546.6 0 11 86 9 546.6 0 9 87 17 546.6 0 17 88 15 546.6 0 15 89 12 546.6 0 12 90 12 546.6 0 12 91 30 546.6 0 30 92 19 546.6 0 19 93 5 546.6 0 5 94 16 546.6 0 16 95 30 546.6 0 30 96 25 546.6 0 25 97 15 546.6 0 15 98 21 546.6 0 21 99 9 546.6 0 9 100 12 546.6 0 12 101 16 546.6 0 16 102 8 546.6 0 8 103 20 546.6 0 20 104 17 546.6 0 17 105 17 546.6 0 17 106 6 546.6 0 6 107 11 546.6 0 11 108 12 546.6 0 12 109 7 546.6 0 7 110 12 546.6 0 12 111 15 546.6 0 15 112 21 546.6 0 21 113 12 546.6 0 12 114 18 546.6 0 18 115 21 546.6 0 21 116 20 546.6 0 20 117 15 546.6 0 15 118 19 546.6 0 19 119 18 546.6 0 18 120 29 546.6 0 29 121 60 546.6 0 60 122 17 546.6 0 17 123 28 546.6 0 28 124 21 546.6 0 21 125 29 546.6 0 29 126 70 546.6 0 70 127 635 546.6 0 635 128 31 546.6 0 31 129 17 546.6 0 17 130 10 546.6 0 10 131 12 546.6 0 12 132 11 546.6 0 11 133 17 546.6 0 17 134 12 546.6 0 12 135 72 546.6 0 72 136 44 546.6 0 44 137 118 546.6 0 118 138 21 546.6 0 21 139 19 546.6 0 19 140 23 546.6 0 23 141 18 546.6 0 18 142 30 546.6 0 30 143 26 546.6 0 26 144 34 546.6 0 34 145 37 546.6 0 37 146 15 546.6 0 15 147 14 546.6 0 14 148 15 546.6 0 15 149 16 546.6 0 16 150 27 546.6 0 27 151 9 546.6 0 9 152 8 546.6 0 8 153 15 546.6 0 15 154 18 546.6 0 18 155 9 546.6 0 9 156 8 546.6 0 8 157 31 546.6 0 31 158 25 546.6 0 25 159 19 546.6 0 19 160 17 546.6 0 17 161 17 546.6 0 17 162 23 546.6 0 23 163 24 546.6 0 24 164 30 546.6 0 30 165 28 546.6 0 28 166 30 546.6 0 30 167 31 546.6 0 31 168 50 546.6 0 50 169 26 546.6 0 26 170 29 546.6 0 29 171 26 546.6 0 26 172 13 546.6 0 13 173 16 546.6 0 16 174 15 546.6 0 15 175 19 546.6 0 19 176 25 546.6 0 25 177 22 546.6 0 22 178 20 546.6 0 20 179 33 546.6 0 33 180 47 546.6 0 47 181 35 546.6 0 35 182 47 546.6 0 47 183 36 546.6 0 36 184 27 546.6 0 27 185 28 546.6 0 28 186 73 546.6 0 73 187 59 546.6 0 59 188 92 546.6 0 92 189 49 546.6 0 49 190 30 546.6 0 30 191 36 546.6 0 36 192 34 546.6 0 34 193 61 546.6 0 61 194 65 546.6 0 65 195 65 546.6 0 65 196 43 546.6 0 43 197 123 546.6 0 123 198 139 546.6 0 139 199 150 546.6 0 150 200 51 546.6 0 51 201 105 546.6 0 105 202 80 546.6 0 80 203 95 546.6 0 95 204 101 546.6 0 101 205 75 546.6 0 75 206 33 546.6 0 33 207 35 546.6 0 35 208 29 546.6 0 29 209 21 546.6 0 21 210 40 546.6 0 40 211 66 546.6 0 66 212 33 546.6 0 33 213 39 546.6 0 39 214 51 546.6 0 51 215 52 546.6 0 52 216 56 546.6 0 56 217 52 546.6 0 52 218 125 546.6 0 125 219 165 546.6 0 165 220 139 546.6 0 139 221 54 546.6 0 54 222 63 546.6 0 63 223 243 546.6 0 243 224 398 546.6 0 398 225 226 546.6 0 226 226 106 546.6 0 106 227 39 546.6 0 39 228 45 546.6 0 45 229 183 546.6 0 183 230 141 546.6 0 141 231 82 546.6 0 82 232 90 546.6 0 90 233 137 546.6 0 137 234 229 546.6 0 229 235 348 546.6 0 348 236 460 546.6 0 460 237 842 546.6 0 842 238 9787 546.6 0 9787 239 2941 546.6 0 2941 240 10463 546.6 0 10463 241 295 546.6 0 295 242 155 546.6 0 155 243 166 546.6 0 166 244 254 546.6 0 254 245 269 546.6 0 269 246 398 546.6 0 398 247 490 546.6 0 490 248 4226 546.6 0 4226 249 24726 546.6 0 24726 250 17119 546.6 0 17119 251 7936 546.6 0 7936 252 56 546.6 0 56 253 26 546.6 0 26 254 21 546.6 0 21 255 19 546.6 0 19 256 27 546.6 0 27 257 26 546.6 0 26 258 20 546.6 0 20 259 91 546.6 0 91 260 30 546.6 0 30 261 25 546.6 0 25 262 12 546.6 0 12 263 112 546.6 0 112 264 60 546.6 0 60 265 24 546.6 0 24 266 40 546.6 0 40 267 24 546.6 0 24 268 30 546.6 0 30 269 47 546.6 0 47 270 41 546.6 0 41 271 63 546.6 0 63 272 33 546.6 0 33 273 42 546.6 0 42 274 22 546.6 0 22 275 48 546.6 0 48 276 20 546.6 0 20 277 37 546.6 0 37 278 67 546.6 0 67 279 106 546.6 0 106 280 173 546.6 0 173 281 200 546.6 0 200 282 433 546.6 0 433 283 885 546.6 0 885 284 2422 546.6 0 2422 285 408 546.6 0 408 286 79 546.6 0 79 287 73 546.6 0 73 288 94 546.6 0 94 289 45 546.6 0 45 290 32 546.6 0 32 291 72 546.6 0 72 292 46 546.6 0 46 293 56 546.6 0 56 294 204 546.6 0 204 295 444 546.6 0 444 296 694 546.6 0 694 297 378 546.6 0 378 298 89 546.6 0 89 299 145 546.6 0 145 300 240 546.6 0 240 301 246 546.6 0 246 Finished in 1.41 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 30,235 Reads with adapters: 4,460 (14.8%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 4,457 (14.7%) Total basepairs processed: 7,087,107 bp Total written (filtered): 1,213,177 bp (17.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4460 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 61 0.0 3 0 58 2 0 1 20 4167 0.0 4 4086 79 2 21 15 0.0 4 2 12 1 22 172 0.0 4 166 4 1 0 1 23 2 0.0 4 0 0 0 2 24 1 0.0 4 1 25 1 0.0 4 1 59 21 0.0 4 0 0 0 0 21 112 1 0.0 4 0 0 0 0 1 127 15 0.0 4 0 0 0 0 15 166 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 4,457 Reads with adapters: 362 (8.1%) Reads written (passing filters): 4,457 (100.0%) Total basepairs processed: 1,213,177 bp Total written (filtered): 1,209,183 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 362 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 3.9% G: 94.5% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 69.6 0 2 4 2 17.4 0 2 9 5 0.0 0 0 5 10 123 0.0 1 9 114 11 226 0.0 1 2 224 12 2 0.0 1 0 2 28 1 0.0 1 1 167 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.