This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 134.75 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 163,004 (5.5%) Reads that were too short: 41,331 (1.4%) Reads written (passing filters): 121,673 (4.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 21,719,761 bp (2.4%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 163004 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 638 720.2 0 638 7 15973 720.2 0 15973 8 352 720.2 0 352 9 16850 720.2 0 16850 10 38 720.2 0 38 11 15 720.2 0 15 12 18 720.2 0 18 13 12 720.2 0 12 14 12 720.2 0 12 15 15 720.2 0 15 16 17 720.2 0 17 17 17 720.2 0 17 18 10 720.2 0 10 19 9 720.2 0 9 20 28 720.2 0 28 21 11 720.2 0 11 22 11 720.2 0 11 23 19 720.2 0 19 24 9 720.2 0 9 25 16 720.2 0 16 26 15 720.2 0 15 27 22 720.2 0 22 28 15 720.2 0 15 29 25 720.2 0 25 30 9 720.2 0 9 31 13 720.2 0 13 32 23 720.2 0 23 33 30 720.2 0 30 34 21 720.2 0 21 35 18 720.2 0 18 36 34 720.2 0 34 37 37 720.2 0 37 38 20 720.2 0 20 39 21 720.2 0 21 40 12 720.2 0 12 41 19 720.2 0 19 42 175 720.2 0 175 43 28 720.2 0 28 44 14 720.2 0 14 45 12 720.2 0 12 46 20 720.2 0 20 47 21 720.2 0 21 48 15 720.2 0 15 49 12 720.2 0 12 50 22 720.2 0 22 51 18 720.2 0 18 52 13 720.2 0 13 53 8 720.2 0 8 54 28 720.2 0 28 55 31 720.2 0 31 56 51 720.2 0 51 57 15 720.2 0 15 58 20 720.2 0 20 59 39 720.2 0 39 60 23 720.2 0 23 61 17 720.2 0 17 62 24 720.2 0 24 63 32 720.2 0 32 64 93 720.2 0 93 65 157 720.2 0 157 66 126 720.2 0 126 67 11 720.2 0 11 68 22 720.2 0 22 69 41 720.2 0 41 70 30 720.2 0 30 71 87 720.2 0 87 72 108 720.2 0 108 73 113 720.2 0 113 74 197 720.2 0 197 75 160 720.2 0 160 76 248 720.2 0 248 77 27 720.2 0 27 78 60 720.2 0 60 79 83 720.2 0 83 80 174 720.2 0 174 81 87 720.2 0 87 82 64 720.2 0 64 83 77 720.2 0 77 84 490 720.2 0 490 85 143 720.2 0 143 86 18 720.2 0 18 87 27 720.2 0 27 88 49 720.2 0 49 89 31 720.2 0 31 90 32 720.2 0 32 91 46 720.2 0 46 92 24 720.2 0 24 93 18 720.2 0 18 94 16 720.2 0 16 95 36 720.2 0 36 96 22 720.2 0 22 97 31 720.2 0 31 98 33 720.2 0 33 99 24 720.2 0 24 100 21 720.2 0 21 101 55 720.2 0 55 102 59 720.2 0 59 103 75 720.2 0 75 104 122 720.2 0 122 105 134 720.2 0 134 106 148 720.2 0 148 107 1656 720.2 0 1656 108 346 720.2 0 346 109 1809 720.2 0 1809 110 68 720.2 0 68 111 102 720.2 0 102 112 76 720.2 0 76 113 94 720.2 0 94 114 66 720.2 0 66 115 106 720.2 0 106 116 137 720.2 0 137 117 222 720.2 0 222 118 116 720.2 0 116 119 145 720.2 0 145 120 406 720.2 0 406 121 791 720.2 0 791 122 881 720.2 0 881 123 223 720.2 0 223 124 279 720.2 0 279 125 347 720.2 0 347 126 241 720.2 0 241 127 139 720.2 0 139 128 118 720.2 0 118 129 59 720.2 0 59 130 99 720.2 0 99 131 94 720.2 0 94 132 75 720.2 0 75 133 66 720.2 0 66 134 58 720.2 0 58 135 89 720.2 0 89 136 86 720.2 0 86 137 109 720.2 0 109 138 110 720.2 0 110 139 124 720.2 0 124 140 65 720.2 0 65 141 127 720.2 0 127 142 91 720.2 0 91 143 145 720.2 0 145 144 96 720.2 0 96 145 85 720.2 0 85 146 190 720.2 0 190 147 143 720.2 0 143 148 113 720.2 0 113 149 103 720.2 0 103 150 90 720.2 0 90 151 98 720.2 0 98 152 96 720.2 0 96 153 97 720.2 0 97 154 90 720.2 0 90 155 91 720.2 0 91 156 77 720.2 0 77 157 133 720.2 0 133 158 71 720.2 0 71 159 109 720.2 0 109 160 154 720.2 0 154 161 110 720.2 0 110 162 110 720.2 0 110 163 119 720.2 0 119 164 217 720.2 0 217 165 215 720.2 0 215 166 1297 720.2 0 1297 167 1031 720.2 0 1031 168 3194 720.2 0 3194 169 283 720.2 0 283 170 1525 720.2 0 1525 171 2392 720.2 0 2392 172 1763 720.2 0 1763 173 219 720.2 0 219 174 160 720.2 0 160 175 193 720.2 0 193 176 249 720.2 0 249 177 358 720.2 0 358 178 347 720.2 0 347 179 463 720.2 0 463 180 763 720.2 0 763 181 17406 720.2 0 17406 182 29258 720.2 0 29258 183 4035 720.2 0 4035 184 1423 720.2 0 1423 185 1131 720.2 0 1131 186 805 720.2 0 805 187 80 720.2 0 80 188 76 720.2 0 76 189 97 720.2 0 97 190 90 720.2 0 90 191 81 720.2 0 81 192 95 720.2 0 95 193 67 720.2 0 67 194 79 720.2 0 79 195 79 720.2 0 79 196 118 720.2 0 118 197 100 720.2 0 100 198 317 720.2 0 317 199 328 720.2 0 328 200 517 720.2 0 517 201 221 720.2 0 221 202 117 720.2 0 117 203 95 720.2 0 95 204 260 720.2 0 260 205 656 720.2 0 656 206 646 720.2 0 646 207 549 720.2 0 549 208 123 720.2 0 123 209 153 720.2 0 153 210 167 720.2 0 167 211 226 720.2 0 226 212 278 720.2 0 278 213 3371 720.2 0 3371 214 2213 720.2 0 2213 215 7838 720.2 0 7838 216 339 720.2 0 339 217 94 720.2 0 94 218 162 720.2 0 162 219 152 720.2 0 152 220 145 720.2 0 145 221 111 720.2 0 111 222 91 720.2 0 91 223 101 720.2 0 101 224 110 720.2 0 110 225 471 720.2 0 471 226 287 720.2 0 287 227 463 720.2 0 463 228 419 720.2 0 419 229 454 720.2 0 454 230 660 720.2 0 660 231 79 720.2 0 79 232 66 720.2 0 66 233 64 720.2 0 64 234 60 720.2 0 60 235 48 720.2 0 48 236 73 720.2 0 73 237 83 720.2 0 83 238 98 720.2 0 98 239 182 720.2 0 182 240 113 720.2 0 113 241 108 720.2 0 108 242 178 720.2 0 178 243 249 720.2 0 249 244 175 720.2 0 175 245 227 720.2 0 227 246 319 720.2 0 319 247 277 720.2 0 277 248 197 720.2 0 197 249 122 720.2 0 122 250 121 720.2 0 121 251 114 720.2 0 114 252 113 720.2 0 113 253 142 720.2 0 142 254 189 720.2 0 189 255 228 720.2 0 228 256 210 720.2 0 210 257 139 720.2 0 139 258 111 720.2 0 111 259 89 720.2 0 89 260 145 720.2 0 145 261 168 720.2 0 168 262 307 720.2 0 307 263 463 720.2 0 463 264 384 720.2 0 384 265 218 720.2 0 218 266 256 720.2 0 256 267 2517 720.2 0 2517 268 2739 720.2 0 2739 269 2889 720.2 0 2889 270 510 720.2 0 510 271 81 720.2 0 81 272 66 720.2 0 66 273 105 720.2 0 105 274 97 720.2 0 97 275 154 720.2 0 154 276 156 720.2 0 156 277 201 720.2 0 201 278 173 720.2 0 173 279 196 720.2 0 196 280 755 720.2 0 755 281 984 720.2 0 984 282 669 720.2 0 669 283 128 720.2 0 128 284 83 720.2 0 83 285 71 720.2 0 71 286 96 720.2 0 96 287 115 720.2 0 115 288 113 720.2 0 113 289 110 720.2 0 110 290 106 720.2 0 106 291 81 720.2 0 81 292 138 720.2 0 138 293 130 720.2 0 130 294 115 720.2 0 115 295 102 720.2 0 102 296 100 720.2 0 100 297 86 720.2 0 86 298 120 720.2 0 120 299 134 720.2 0 134 300 128 720.2 0 128 301 217 720.2 0 217 Finished in 6.96 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 121,673 Reads with adapters: 17,914 (14.7%) Reads that were too short: 145 (0.1%) Reads written (passing filters): 17,769 (14.6%) Total basepairs processed: 21,719,761 bp Total written (filtered): 4,835,684 bp (22.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17914 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 5 2 16 167 0.0 3 0 147 15 5 17 17139 0.0 3 16194 799 123 23 18 54 0.0 3 1 40 3 10 19 28 0.0 3 8 0 12 8 20 54 0.0 3 1 0 0 53 21 161 0.0 3 0 0 0 161 24 49 0.0 3 0 0 2 47 25 7 0.0 3 0 0 0 7 27 4 0.0 3 0 0 1 3 28 5 0.0 3 0 0 0 5 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 47 6 0.0 3 0 0 0 6 48 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 50 4 0.0 3 0 0 0 4 51 4 0.0 3 0 0 1 3 52 3 0.0 3 0 0 0 3 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 1 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 71 20 0.0 3 0 0 0 20 72 14 0.0 3 0 0 0 14 73 2 0.0 3 0 0 0 2 74 6 0.0 3 0 0 2 4 75 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 9 0.0 3 0 0 0 9 85 3 0.0 3 0 0 0 3 86 2 0.0 3 0 0 0 2 89 4 0.0 3 0 0 0 4 90 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 1 1 97 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 1 1 101 2 0.0 3 0 0 0 2 102 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 1 119 3 0.0 3 0 0 0 3 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 17 0.0 3 0 0 0 17 123 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 1 1 131 2 0.0 3 0 0 0 2 133 8 0.0 3 0 0 0 8 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 1 0 1 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 173 4 0.0 3 0 0 0 4 175 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 1 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.01 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 17,769 Reads with adapters: 423 (2.4%) Reads written (passing filters): 17,769 (100.0%) Total basepairs processed: 4,835,684 bp Total written (filtered): 4,834,370 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 423 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.4% C: 11.1% G: 28.8% T: 5.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 379 277.6 0 379 4 43 69.4 0 43 5 1 17.4 0 1