This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 132.71 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 60,551 (2.1%) Reads that were too short: 31,912 (1.1%) Reads written (passing filters): 28,639 (1.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 4,838,048 bp (0.5%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 60551 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 30 720.2 0 30 7 67 720.2 0 67 8 439 720.2 0 439 9 379 720.2 0 379 10 35 720.2 0 35 11 31 720.2 0 31 12 40 720.2 0 40 13 40 720.2 0 40 14 47 720.2 0 47 15 23 720.2 0 23 16 25 720.2 0 25 17 31 720.2 0 31 18 33 720.2 0 33 19 22 720.2 0 22 20 39 720.2 0 39 21 36 720.2 0 36 22 30 720.2 0 30 23 32 720.2 0 32 24 37 720.2 0 37 25 38 720.2 0 38 26 22 720.2 0 22 27 28 720.2 0 28 28 27 720.2 0 27 29 34 720.2 0 34 30 27 720.2 0 27 31 27 720.2 0 27 32 23 720.2 0 23 33 29 720.2 0 29 34 34 720.2 0 34 35 39 720.2 0 39 36 48 720.2 0 48 37 43 720.2 0 43 38 40 720.2 0 40 39 39 720.2 0 39 40 48 720.2 0 48 41 57 720.2 0 57 42 43 720.2 0 43 43 24 720.2 0 24 44 33 720.2 0 33 45 40 720.2 0 40 46 50 720.2 0 50 47 24 720.2 0 24 48 39 720.2 0 39 49 43 720.2 0 43 50 55 720.2 0 55 51 45 720.2 0 45 52 41 720.2 0 41 53 75 720.2 0 75 54 55 720.2 0 55 55 128 720.2 0 128 56 61 720.2 0 61 57 36 720.2 0 36 58 54 720.2 0 54 59 65 720.2 0 65 60 31 720.2 0 31 61 60 720.2 0 60 62 39 720.2 0 39 63 118 720.2 0 118 64 100 720.2 0 100 65 130 720.2 0 130 66 38 720.2 0 38 67 55 720.2 0 55 68 76 720.2 0 76 69 115 720.2 0 115 70 57 720.2 0 57 71 88 720.2 0 88 72 475 720.2 0 475 73 1332 720.2 0 1332 74 1060 720.2 0 1060 75 1144 720.2 0 1144 76 32 720.2 0 32 77 37 720.2 0 37 78 40 720.2 0 40 79 51 720.2 0 51 80 51 720.2 0 51 81 19 720.2 0 19 82 45 720.2 0 45 83 37 720.2 0 37 84 25 720.2 0 25 85 56 720.2 0 56 86 71 720.2 0 71 87 79 720.2 0 79 88 201 720.2 0 201 89 80 720.2 0 80 90 36 720.2 0 36 91 67 720.2 0 67 92 55 720.2 0 55 93 25 720.2 0 25 94 41 720.2 0 41 95 45 720.2 0 45 96 53 720.2 0 53 97 34 720.2 0 34 98 35 720.2 0 35 99 41 720.2 0 41 100 87 720.2 0 87 101 35 720.2 0 35 102 40 720.2 0 40 103 41 720.2 0 41 104 38 720.2 0 38 105 204 720.2 0 204 106 54 720.2 0 54 107 80 720.2 0 80 108 72 720.2 0 72 109 67 720.2 0 67 110 52 720.2 0 52 111 60 720.2 0 60 112 30 720.2 0 30 113 33 720.2 0 33 114 33 720.2 0 33 115 54 720.2 0 54 116 44 720.2 0 44 117 30 720.2 0 30 118 50 720.2 0 50 119 42 720.2 0 42 120 32 720.2 0 32 121 46 720.2 0 46 122 40 720.2 0 40 123 32 720.2 0 32 124 33 720.2 0 33 125 29 720.2 0 29 126 48 720.2 0 48 127 45 720.2 0 45 128 98 720.2 0 98 129 100 720.2 0 100 130 78 720.2 0 78 131 57 720.2 0 57 132 50 720.2 0 50 133 51 720.2 0 51 134 71 720.2 0 71 135 57 720.2 0 57 136 96 720.2 0 96 137 108 720.2 0 108 138 140 720.2 0 140 139 37 720.2 0 37 140 40 720.2 0 40 141 80 720.2 0 80 142 59 720.2 0 59 143 86 720.2 0 86 144 69 720.2 0 69 145 225 720.2 0 225 146 354 720.2 0 354 147 617 720.2 0 617 148 278 720.2 0 278 149 123 720.2 0 123 150 116 720.2 0 116 151 76 720.2 0 76 152 57 720.2 0 57 153 78 720.2 0 78 154 92 720.2 0 92 155 146 720.2 0 146 156 247 720.2 0 247 157 192 720.2 0 192 158 645 720.2 0 645 159 695 720.2 0 695 160 1298 720.2 0 1298 161 165 720.2 0 165 162 130 720.2 0 130 163 145 720.2 0 145 164 62 720.2 0 62 165 51 720.2 0 51 166 85 720.2 0 85 167 174 720.2 0 174 168 99 720.2 0 99 169 136 720.2 0 136 170 119 720.2 0 119 171 170 720.2 0 170 172 132 720.2 0 132 173 120 720.2 0 120 174 1377 720.2 0 1377 175 615 720.2 0 615 176 1905 720.2 0 1905 177 146 720.2 0 146 178 71 720.2 0 71 179 54 720.2 0 54 180 110 720.2 0 110 181 169 720.2 0 169 182 197 720.2 0 197 183 102 720.2 0 102 184 107 720.2 0 107 185 239 720.2 0 239 186 313 720.2 0 313 187 1579 720.2 0 1579 188 994 720.2 0 994 189 1307 720.2 0 1307 190 71 720.2 0 71 191 46 720.2 0 46 192 58 720.2 0 58 193 52 720.2 0 52 194 58 720.2 0 58 195 65 720.2 0 65 196 67 720.2 0 67 197 53 720.2 0 53 198 36 720.2 0 36 199 36 720.2 0 36 200 72 720.2 0 72 201 81 720.2 0 81 202 78 720.2 0 78 203 81 720.2 0 81 204 165 720.2 0 165 205 119 720.2 0 119 206 118 720.2 0 118 207 90 720.2 0 90 208 54 720.2 0 54 209 67 720.2 0 67 210 46 720.2 0 46 211 56 720.2 0 56 212 57 720.2 0 57 213 55 720.2 0 55 214 57 720.2 0 57 215 70 720.2 0 70 216 88 720.2 0 88 217 81 720.2 0 81 218 95 720.2 0 95 219 73 720.2 0 73 220 77 720.2 0 77 221 70 720.2 0 70 222 55 720.2 0 55 223 81 720.2 0 81 224 75 720.2 0 75 225 85 720.2 0 85 226 69 720.2 0 69 227 134 720.2 0 134 228 100 720.2 0 100 229 213 720.2 0 213 230 76 720.2 0 76 231 117 720.2 0 117 232 131 720.2 0 131 233 173 720.2 0 173 234 110 720.2 0 110 235 98 720.2 0 98 236 102 720.2 0 102 237 138 720.2 0 138 238 173 720.2 0 173 239 197 720.2 0 197 240 130 720.2 0 130 241 88 720.2 0 88 242 86 720.2 0 86 243 89 720.2 0 89 244 124 720.2 0 124 245 132 720.2 0 132 246 139 720.2 0 139 247 141 720.2 0 141 248 293 720.2 0 293 249 351 720.2 0 351 250 561 720.2 0 561 251 13556 720.2 0 13556 252 1808 720.2 0 1808 253 1662 720.2 0 1662 254 141 720.2 0 141 255 127 720.2 0 127 256 89 720.2 0 89 257 63 720.2 0 63 258 119 720.2 0 119 259 149 720.2 0 149 260 259 720.2 0 259 261 325 720.2 0 325 262 528 720.2 0 528 263 441 720.2 0 441 264 251 720.2 0 251 265 57 720.2 0 57 266 41 720.2 0 41 267 65 720.2 0 65 268 159 720.2 0 159 269 263 720.2 0 263 270 144 720.2 0 144 271 97 720.2 0 97 272 209 720.2 0 209 273 130 720.2 0 130 274 150 720.2 0 150 275 153 720.2 0 153 276 112 720.2 0 112 277 129 720.2 0 129 278 153 720.2 0 153 279 250 720.2 0 250 280 216 720.2 0 216 281 241 720.2 0 241 282 232 720.2 0 232 283 211 720.2 0 211 284 100 720.2 0 100 285 190 720.2 0 190 286 179 720.2 0 179 287 114 720.2 0 114 288 109 720.2 0 109 289 81 720.2 0 81 290 110 720.2 0 110 291 241 720.2 0 241 292 219 720.2 0 219 293 160 720.2 0 160 294 180 720.2 0 180 295 305 720.2 0 305 296 164 720.2 0 164 297 228 720.2 0 228 298 223 720.2 0 223 299 316 720.2 0 316 300 332 720.2 0 332 301 273 720.2 0 273 Finished in 1.53 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 28,639 Reads with adapters: 535 (1.9%) Reads that were too short: 71 (0.2%) Reads written (passing filters): 464 (1.6%) Total basepairs processed: 4,838,048 bp Total written (filtered): 119,106 bp (2.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 535 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 16 0.0 3 0 6 0 10 17 379 0.0 3 358 14 6 1 18 1 0.0 3 0 0 1 19 12 0.0 3 8 2 1 1 20 3 0.0 3 1 0 0 2 21 1 0.0 3 1 22 2 0.0 3 0 0 0 2 25 2 0.0 3 0 0 0 2 26 3 0.0 3 0 0 1 2 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 32 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 5 0.0 3 0 0 0 5 38 1 0.0 3 0 0 0 1 40 1 0.0 3 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 51 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 1 1 57 3 0.0 3 0 0 0 3 58 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 87 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 1 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 5 0.0 3 0 0 0 5 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 1 174 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 464 Reads with adapters: 0 (0.0%) Reads written (passing filters): 464 (100.0%) Total basepairs processed: 119,106 bp Total written (filtered): 119,106 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.