This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 133.53 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 55,087 (1.9%) Reads that were too short: 17,695 (0.6%) Reads written (passing filters): 37,392 (1.3%) Total basepairs processed: 887,907,860 bp Total written (filtered): 6,238,681 bp (0.7%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 55087 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 720.2 0 129 7 3166 720.2 0 3166 8 2686 720.2 0 2686 9 17 720.2 0 17 10 31 720.2 0 31 11 59 720.2 0 59 12 38 720.2 0 38 13 167 720.2 0 167 14 100 720.2 0 100 15 278 720.2 0 278 16 44 720.2 0 44 17 13 720.2 0 13 18 14 720.2 0 14 19 13 720.2 0 13 20 17 720.2 0 17 21 22 720.2 0 22 22 10 720.2 0 10 23 21 720.2 0 21 24 34 720.2 0 34 25 75 720.2 0 75 26 79 720.2 0 79 27 184 720.2 0 184 28 18 720.2 0 18 29 12 720.2 0 12 30 20 720.2 0 20 31 15 720.2 0 15 32 20 720.2 0 20 33 13 720.2 0 13 34 7 720.2 0 7 35 20 720.2 0 20 36 15 720.2 0 15 37 17 720.2 0 17 38 18 720.2 0 18 39 24 720.2 0 24 40 10 720.2 0 10 41 14 720.2 0 14 42 19 720.2 0 19 43 30 720.2 0 30 44 12 720.2 0 12 45 16 720.2 0 16 46 10 720.2 0 10 47 21 720.2 0 21 48 24 720.2 0 24 49 22 720.2 0 22 50 31 720.2 0 31 51 15 720.2 0 15 52 5 720.2 0 5 53 16 720.2 0 16 54 34 720.2 0 34 55 24 720.2 0 24 56 32 720.2 0 32 57 30 720.2 0 30 58 28 720.2 0 28 59 138 720.2 0 138 60 46 720.2 0 46 61 77 720.2 0 77 62 41 720.2 0 41 63 21 720.2 0 21 64 24 720.2 0 24 65 21 720.2 0 21 66 24 720.2 0 24 67 10 720.2 0 10 68 23 720.2 0 23 69 17 720.2 0 17 70 15 720.2 0 15 71 27 720.2 0 27 72 16 720.2 0 16 73 21 720.2 0 21 74 22 720.2 0 22 75 29 720.2 0 29 76 21 720.2 0 21 77 30 720.2 0 30 78 34 720.2 0 34 79 38 720.2 0 38 80 90 720.2 0 90 81 58 720.2 0 58 82 697 720.2 0 697 83 266 720.2 0 266 84 461 720.2 0 461 85 7 720.2 0 7 86 27 720.2 0 27 87 25 720.2 0 25 88 13 720.2 0 13 89 26 720.2 0 26 90 21 720.2 0 21 91 21 720.2 0 21 92 13 720.2 0 13 93 32 720.2 0 32 94 23 720.2 0 23 95 24 720.2 0 24 96 34 720.2 0 34 97 33 720.2 0 33 98 19 720.2 0 19 99 27 720.2 0 27 100 19 720.2 0 19 101 30 720.2 0 30 102 75 720.2 0 75 103 96 720.2 0 96 104 60 720.2 0 60 105 54 720.2 0 54 106 79 720.2 0 79 107 189 720.2 0 189 108 81 720.2 0 81 109 29 720.2 0 29 110 168 720.2 0 168 111 63 720.2 0 63 112 104 720.2 0 104 113 50 720.2 0 50 114 33 720.2 0 33 115 30 720.2 0 30 116 26 720.2 0 26 117 21 720.2 0 21 118 32 720.2 0 32 119 39 720.2 0 39 120 70 720.2 0 70 121 36 720.2 0 36 122 174 720.2 0 174 123 288 720.2 0 288 124 183 720.2 0 183 125 25 720.2 0 25 126 28 720.2 0 28 127 41 720.2 0 41 128 46 720.2 0 46 129 38 720.2 0 38 130 26 720.2 0 26 131 41 720.2 0 41 132 38 720.2 0 38 133 49 720.2 0 49 134 53 720.2 0 53 135 49 720.2 0 49 136 69 720.2 0 69 137 142 720.2 0 142 138 253 720.2 0 253 139 161 720.2 0 161 140 105 720.2 0 105 141 79 720.2 0 79 142 102 720.2 0 102 143 33 720.2 0 33 144 33 720.2 0 33 145 19 720.2 0 19 146 53 720.2 0 53 147 109 720.2 0 109 148 49 720.2 0 49 149 36 720.2 0 36 150 50 720.2 0 50 151 39 720.2 0 39 152 40 720.2 0 40 153 46 720.2 0 46 154 47 720.2 0 47 155 57 720.2 0 57 156 76 720.2 0 76 157 85 720.2 0 85 158 120 720.2 0 120 159 141 720.2 0 141 160 500 720.2 0 500 161 724 720.2 0 724 162 979 720.2 0 979 163 263 720.2 0 263 164 96 720.2 0 96 165 78 720.2 0 78 166 31 720.2 0 31 167 64 720.2 0 64 168 62 720.2 0 62 169 62 720.2 0 62 170 110 720.2 0 110 171 31 720.2 0 31 172 58 720.2 0 58 173 80 720.2 0 80 174 97 720.2 0 97 175 124 720.2 0 124 176 119 720.2 0 119 177 124 720.2 0 124 178 88 720.2 0 88 179 95 720.2 0 95 180 389 720.2 0 389 181 893 720.2 0 893 182 1215 720.2 0 1215 183 667 720.2 0 667 184 162 720.2 0 162 185 235 720.2 0 235 186 337 720.2 0 337 187 4884 720.2 0 4884 188 2593 720.2 0 2593 189 6900 720.2 0 6900 190 50 720.2 0 50 191 43 720.2 0 43 192 32 720.2 0 32 193 38 720.2 0 38 194 44 720.2 0 44 195 25 720.2 0 25 196 34 720.2 0 34 197 62 720.2 0 62 198 42 720.2 0 42 199 52 720.2 0 52 200 60 720.2 0 60 201 42 720.2 0 42 202 85 720.2 0 85 203 135 720.2 0 135 204 187 720.2 0 187 205 156 720.2 0 156 206 253 720.2 0 253 207 167 720.2 0 167 208 85 720.2 0 85 209 49 720.2 0 49 210 176 720.2 0 176 211 357 720.2 0 357 212 611 720.2 0 611 213 249 720.2 0 249 214 153 720.2 0 153 215 101 720.2 0 101 216 48 720.2 0 48 217 79 720.2 0 79 218 51 720.2 0 51 219 153 720.2 0 153 220 226 720.2 0 226 221 168 720.2 0 168 222 130 720.2 0 130 223 66 720.2 0 66 224 78 720.2 0 78 225 176 720.2 0 176 226 189 720.2 0 189 227 173 720.2 0 173 228 107 720.2 0 107 229 168 720.2 0 168 230 1036 720.2 0 1036 231 1649 720.2 0 1649 232 1195 720.2 0 1195 233 121 720.2 0 121 234 40 720.2 0 40 235 29 720.2 0 29 236 27 720.2 0 27 237 23 720.2 0 23 238 33 720.2 0 33 239 43 720.2 0 43 240 54 720.2 0 54 241 73 720.2 0 73 242 43 720.2 0 43 243 31 720.2 0 31 244 40 720.2 0 40 245 42 720.2 0 42 246 31 720.2 0 31 247 26 720.2 0 26 248 21 720.2 0 21 249 29 720.2 0 29 250 27 720.2 0 27 251 28 720.2 0 28 252 33 720.2 0 33 253 60 720.2 0 60 254 87 720.2 0 87 255 58 720.2 0 58 256 64 720.2 0 64 257 57 720.2 0 57 258 62 720.2 0 62 259 52 720.2 0 52 260 63 720.2 0 63 261 50 720.2 0 50 262 54 720.2 0 54 263 65 720.2 0 65 264 67 720.2 0 67 265 55 720.2 0 55 266 54 720.2 0 54 267 57 720.2 0 57 268 94 720.2 0 94 269 78 720.2 0 78 270 47 720.2 0 47 271 60 720.2 0 60 272 62 720.2 0 62 273 124 720.2 0 124 274 160 720.2 0 160 275 475 720.2 0 475 276 401 720.2 0 401 277 672 720.2 0 672 278 250 720.2 0 250 279 311 720.2 0 311 280 301 720.2 0 301 281 210 720.2 0 210 282 84 720.2 0 84 283 134 720.2 0 134 284 165 720.2 0 165 285 195 720.2 0 195 286 100 720.2 0 100 287 108 720.2 0 108 288 225 720.2 0 225 289 683 720.2 0 683 290 926 720.2 0 926 291 218 720.2 0 218 292 59 720.2 0 59 293 67 720.2 0 67 294 124 720.2 0 124 295 115 720.2 0 115 296 90 720.2 0 90 297 72 720.2 0 72 298 129 720.2 0 129 299 86 720.2 0 86 300 273 720.2 0 273 301 442 720.2 0 442 Finished in 2.05 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 37,392 Reads with adapters: 3,462 (9.3%) Reads that were too short: 103 (0.3%) Reads written (passing filters): 3,359 (9.0%) Total basepairs processed: 6,238,681 bp Total written (filtered): 892,754 bp (14.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3462 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 37 0.0 3 0 31 4 2 17 3062 0.0 3 2894 138 24 6 18 22 0.0 3 6 8 0 8 19 6 0.0 3 0 0 3 3 20 7 0.0 3 0 0 0 7 24 1 0.0 3 1 29 1 0.0 3 0 0 1 31 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 35 5 0.0 3 0 0 0 5 36 2 0.0 3 0 0 0 2 37 6 0.0 3 0 0 0 6 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 13 0.0 3 0 0 0 13 55 1 0.0 3 0 0 0 1 57 8 0.0 3 0 0 1 7 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 62 9 0.0 3 0 0 0 9 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 69 15 0.0 3 0 0 0 15 70 3 0.0 3 0 0 0 3 72 3 0.0 3 0 0 1 2 73 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 1 101 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 4 0.0 3 0 0 1 3 114 3 0.0 3 0 0 0 3 120 2 0.0 3 0 0 0 2 123 7 0.0 3 0 0 1 6 126 5 0.0 3 0 0 1 4 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 141 164 0.0 3 0 0 0 164 146 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 1 2 181 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 10 0.0 3 0 0 2 8 263 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 3,359 Reads with adapters: 28 (0.8%) Reads written (passing filters): 3,359 (100.0%) Total basepairs processed: 892,754 bp Total written (filtered): 892,670 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.7% C: 46.4% G: 10.7% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 28 52.5 0 28