This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 131.99 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 44,174 (1.5%) Reads that were too short: 10,096 (0.3%) Reads written (passing filters): 34,078 (1.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 5,732,352 bp (0.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 44174 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 87 720.2 0 87 7 2873 720.2 0 2873 8 3247 720.2 0 3247 9 12 720.2 0 12 10 8 720.2 0 8 11 12 720.2 0 12 12 15 720.2 0 15 13 92 720.2 0 92 14 82 720.2 0 82 15 146 720.2 0 146 16 44 720.2 0 44 17 6 720.2 0 6 18 7 720.2 0 7 19 4 720.2 0 4 20 7 720.2 0 7 21 10 720.2 0 10 22 8 720.2 0 8 23 17 720.2 0 17 24 7 720.2 0 7 25 183 720.2 0 183 26 89 720.2 0 89 27 7 720.2 0 7 28 6 720.2 0 6 29 6 720.2 0 6 30 14 720.2 0 14 31 11 720.2 0 11 32 10 720.2 0 10 33 12 720.2 0 12 34 6 720.2 0 6 35 9 720.2 0 9 36 8 720.2 0 8 37 5 720.2 0 5 38 11 720.2 0 11 39 4 720.2 0 4 40 14 720.2 0 14 41 19 720.2 0 19 42 4 720.2 0 4 43 7 720.2 0 7 44 6 720.2 0 6 45 6 720.2 0 6 46 10 720.2 0 10 47 17 720.2 0 17 48 25 720.2 0 25 49 17 720.2 0 17 50 10 720.2 0 10 51 10 720.2 0 10 52 6 720.2 0 6 53 19 720.2 0 19 54 13 720.2 0 13 55 18 720.2 0 18 56 25 720.2 0 25 57 23 720.2 0 23 58 19 720.2 0 19 59 103 720.2 0 103 60 35 720.2 0 35 61 52 720.2 0 52 62 22 720.2 0 22 63 17 720.2 0 17 64 10 720.2 0 10 65 10 720.2 0 10 66 12 720.2 0 12 67 14 720.2 0 14 68 17 720.2 0 17 69 13 720.2 0 13 70 19 720.2 0 19 71 28 720.2 0 28 72 19 720.2 0 19 73 15 720.2 0 15 74 12 720.2 0 12 75 13 720.2 0 13 76 22 720.2 0 22 77 19 720.2 0 19 78 27 720.2 0 27 79 19 720.2 0 19 80 53 720.2 0 53 81 70 720.2 0 70 82 1219 720.2 0 1219 83 420 720.2 0 420 84 812 720.2 0 812 85 11 720.2 0 11 86 24 720.2 0 24 87 19 720.2 0 19 88 15 720.2 0 15 89 24 720.2 0 24 90 23 720.2 0 23 91 29 720.2 0 29 92 25 720.2 0 25 93 20 720.2 0 20 94 32 720.2 0 32 95 41 720.2 0 41 96 32 720.2 0 32 97 24 720.2 0 24 98 27 720.2 0 27 99 13 720.2 0 13 100 18 720.2 0 18 101 21 720.2 0 21 102 39 720.2 0 39 103 38 720.2 0 38 104 45 720.2 0 45 105 43 720.2 0 43 106 72 720.2 0 72 107 179 720.2 0 179 108 90 720.2 0 90 109 22 720.2 0 22 110 170 720.2 0 170 111 41 720.2 0 41 112 89 720.2 0 89 113 49 720.2 0 49 114 25 720.2 0 25 115 28 720.2 0 28 116 17 720.2 0 17 117 24 720.2 0 24 118 32 720.2 0 32 119 38 720.2 0 38 120 39 720.2 0 39 121 18 720.2 0 18 122 137 720.2 0 137 123 137 720.2 0 137 124 109 720.2 0 109 125 25 720.2 0 25 126 21 720.2 0 21 127 25 720.2 0 25 128 31 720.2 0 31 129 32 720.2 0 32 130 22 720.2 0 22 131 40 720.2 0 40 132 25 720.2 0 25 133 45 720.2 0 45 134 40 720.2 0 40 135 27 720.2 0 27 136 24 720.2 0 24 137 66 720.2 0 66 138 79 720.2 0 79 139 69 720.2 0 69 140 66 720.2 0 66 141 48 720.2 0 48 142 53 720.2 0 53 143 29 720.2 0 29 144 26 720.2 0 26 145 24 720.2 0 24 146 40 720.2 0 40 147 115 720.2 0 115 148 42 720.2 0 42 149 19 720.2 0 19 150 26 720.2 0 26 151 30 720.2 0 30 152 38 720.2 0 38 153 29 720.2 0 29 154 41 720.2 0 41 155 36 720.2 0 36 156 31 720.2 0 31 157 45 720.2 0 45 158 55 720.2 0 55 159 66 720.2 0 66 160 229 720.2 0 229 161 191 720.2 0 191 162 334 720.2 0 334 163 57 720.2 0 57 164 49 720.2 0 49 165 30 720.2 0 30 166 29 720.2 0 29 167 23 720.2 0 23 168 42 720.2 0 42 169 49 720.2 0 49 170 88 720.2 0 88 171 30 720.2 0 30 172 38 720.2 0 38 173 56 720.2 0 56 174 61 720.2 0 61 175 85 720.2 0 85 176 51 720.2 0 51 177 55 720.2 0 55 178 36 720.2 0 36 179 39 720.2 0 39 180 148 720.2 0 148 181 276 720.2 0 276 182 357 720.2 0 357 183 201 720.2 0 201 184 141 720.2 0 141 185 223 720.2 0 223 186 317 720.2 0 317 187 5756 720.2 0 5756 188 2837 720.2 0 2837 189 8133 720.2 0 8133 190 59 720.2 0 59 191 28 720.2 0 28 192 44 720.2 0 44 193 20 720.2 0 20 194 30 720.2 0 30 195 26 720.2 0 26 196 24 720.2 0 24 197 28 720.2 0 28 198 34 720.2 0 34 199 36 720.2 0 36 200 33 720.2 0 33 201 41 720.2 0 41 202 26 720.2 0 26 203 61 720.2 0 61 204 62 720.2 0 62 205 69 720.2 0 69 206 100 720.2 0 100 207 82 720.2 0 82 208 42 720.2 0 42 209 30 720.2 0 30 210 69 720.2 0 69 211 133 720.2 0 133 212 211 720.2 0 211 213 98 720.2 0 98 214 73 720.2 0 73 215 43 720.2 0 43 216 40 720.2 0 40 217 44 720.2 0 44 218 35 720.2 0 35 219 70 720.2 0 70 220 117 720.2 0 117 221 123 720.2 0 123 222 50 720.2 0 50 223 44 720.2 0 44 224 55 720.2 0 55 225 95 720.2 0 95 226 123 720.2 0 123 227 71 720.2 0 71 228 64 720.2 0 64 229 69 720.2 0 69 230 446 720.2 0 446 231 655 720.2 0 655 232 467 720.2 0 467 233 75 720.2 0 75 234 31 720.2 0 31 235 26 720.2 0 26 236 31 720.2 0 31 237 37 720.2 0 37 238 24 720.2 0 24 239 43 720.2 0 43 240 49 720.2 0 49 241 45 720.2 0 45 242 36 720.2 0 36 243 44 720.2 0 44 244 39 720.2 0 39 245 30 720.2 0 30 246 27 720.2 0 27 247 14 720.2 0 14 248 26 720.2 0 26 249 26 720.2 0 26 250 31 720.2 0 31 251 39 720.2 0 39 252 29 720.2 0 29 253 34 720.2 0 34 254 66 720.2 0 66 255 47 720.2 0 47 256 34 720.2 0 34 257 52 720.2 0 52 258 50 720.2 0 50 259 33 720.2 0 33 260 55 720.2 0 55 261 50 720.2 0 50 262 92 720.2 0 92 263 46 720.2 0 46 264 52 720.2 0 52 265 39 720.2 0 39 266 34 720.2 0 34 267 49 720.2 0 49 268 52 720.2 0 52 269 53 720.2 0 53 270 35 720.2 0 35 271 42 720.2 0 42 272 35 720.2 0 35 273 63 720.2 0 63 274 108 720.2 0 108 275 442 720.2 0 442 276 298 720.2 0 298 277 727 720.2 0 727 278 188 720.2 0 188 279 152 720.2 0 152 280 182 720.2 0 182 281 119 720.2 0 119 282 54 720.2 0 54 283 62 720.2 0 62 284 57 720.2 0 57 285 95 720.2 0 95 286 41 720.2 0 41 287 66 720.2 0 66 288 128 720.2 0 128 289 408 720.2 0 408 290 583 720.2 0 583 291 130 720.2 0 130 292 30 720.2 0 30 293 75 720.2 0 75 294 69 720.2 0 69 295 69 720.2 0 69 296 80 720.2 0 80 297 70 720.2 0 70 298 56 720.2 0 56 299 85 720.2 0 85 300 192 720.2 0 192 301 148 720.2 0 148 Finished in 1.97 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 34,078 Reads with adapters: 3,416 (10.0%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 3,413 (10.0%) Total basepairs processed: 5,732,352 bp Total written (filtered): 924,569 bp (16.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3416 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 23 0.0 3 0 21 0 1 1 20 3291 0.0 4 3233 55 3 21 22 0.0 4 10 12 22 4 0.0 4 3 0 1 23 3 0.0 4 0 0 0 3 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 64 0.0 4 0 0 0 0 64 128 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 3,413 Reads with adapters: 267 (7.8%) Reads written (passing filters): 3,413 (100.0%) Total basepairs processed: 924,569 bp Total written (filtered): 921,085 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 267 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 2.2% G: 94.8% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 53.3 0 1 9 14 0.0 0 0 14 10 66 0.0 1 2 64 11 182 0.0 1 0 182 21 1 0.0 1 1 224 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.