This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 130.59 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 80,778 (2.7%) Reads that were too short: 26,648 (0.9%) Reads written (passing filters): 54,130 (1.8%) Total basepairs processed: 887,907,860 bp Total written (filtered): 8,619,303 bp (1.0%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 80778 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 122 720.2 0 122 7 105 720.2 0 105 8 29 720.2 0 29 9 147 720.2 0 147 10 695 720.2 0 695 11 26 720.2 0 26 12 18 720.2 0 18 13 23 720.2 0 23 14 34 720.2 0 34 15 17 720.2 0 17 16 23 720.2 0 23 17 18 720.2 0 18 18 23 720.2 0 23 19 20 720.2 0 20 20 25 720.2 0 25 21 18 720.2 0 18 22 25 720.2 0 25 23 24 720.2 0 24 24 22 720.2 0 22 25 22 720.2 0 22 26 20 720.2 0 20 27 26 720.2 0 26 28 24 720.2 0 24 29 30 720.2 0 30 30 18 720.2 0 18 31 28 720.2 0 28 32 22 720.2 0 22 33 30 720.2 0 30 34 32 720.2 0 32 35 56 720.2 0 56 36 33 720.2 0 33 37 40 720.2 0 40 38 110 720.2 0 110 39 42 720.2 0 42 40 174 720.2 0 174 41 38 720.2 0 38 42 39 720.2 0 39 43 50 720.2 0 50 44 28 720.2 0 28 45 35 720.2 0 35 46 30 720.2 0 30 47 37 720.2 0 37 48 18 720.2 0 18 49 35 720.2 0 35 50 43 720.2 0 43 51 26 720.2 0 26 52 28 720.2 0 28 53 202 720.2 0 202 54 117 720.2 0 117 55 200 720.2 0 200 56 209 720.2 0 209 57 2950 720.2 0 2950 58 69 720.2 0 69 59 30 720.2 0 30 60 44 720.2 0 44 61 23 720.2 0 23 62 28 720.2 0 28 63 42 720.2 0 42 64 28 720.2 0 28 65 25 720.2 0 25 66 98 720.2 0 98 67 53 720.2 0 53 68 120 720.2 0 120 69 89 720.2 0 89 70 474 720.2 0 474 71 78 720.2 0 78 72 102 720.2 0 102 73 62 720.2 0 62 74 47 720.2 0 47 75 26 720.2 0 26 76 27 720.2 0 27 77 20 720.2 0 20 78 29 720.2 0 29 79 24 720.2 0 24 80 32 720.2 0 32 81 31 720.2 0 31 82 31 720.2 0 31 83 25 720.2 0 25 84 26 720.2 0 26 85 23 720.2 0 23 86 44 720.2 0 44 87 38 720.2 0 38 88 40 720.2 0 40 89 30 720.2 0 30 90 30 720.2 0 30 91 51 720.2 0 51 92 46 720.2 0 46 93 43 720.2 0 43 94 58 720.2 0 58 95 73 720.2 0 73 96 32 720.2 0 32 97 17 720.2 0 17 98 18 720.2 0 18 99 33 720.2 0 33 100 27 720.2 0 27 101 45 720.2 0 45 102 55 720.2 0 55 103 126 720.2 0 126 104 114 720.2 0 114 105 191 720.2 0 191 106 86 720.2 0 86 107 58 720.2 0 58 108 39 720.2 0 39 109 57 720.2 0 57 110 50 720.2 0 50 111 72 720.2 0 72 112 89 720.2 0 89 113 140 720.2 0 140 114 49 720.2 0 49 115 68 720.2 0 68 116 66 720.2 0 66 117 35 720.2 0 35 118 35 720.2 0 35 119 42 720.2 0 42 120 81 720.2 0 81 121 59 720.2 0 59 122 61 720.2 0 61 123 108 720.2 0 108 124 111 720.2 0 111 125 1377 720.2 0 1377 126 596 720.2 0 596 127 1647 720.2 0 1647 128 58 720.2 0 58 129 59 720.2 0 59 130 99 720.2 0 99 131 66 720.2 0 66 132 68 720.2 0 68 133 98 720.2 0 98 134 86 720.2 0 86 135 90 720.2 0 90 136 58 720.2 0 58 137 81 720.2 0 81 138 175 720.2 0 175 139 95 720.2 0 95 140 77 720.2 0 77 141 68 720.2 0 68 142 79 720.2 0 79 143 85 720.2 0 85 144 290 720.2 0 290 145 687 720.2 0 687 146 911 720.2 0 911 147 407 720.2 0 407 148 102 720.2 0 102 149 110 720.2 0 110 150 405 720.2 0 405 151 238 720.2 0 238 152 452 720.2 0 452 153 129 720.2 0 129 154 227 720.2 0 227 155 433 720.2 0 433 156 826 720.2 0 826 157 1124 720.2 0 1124 158 1751 720.2 0 1751 159 1091 720.2 0 1091 160 6665 720.2 0 6665 161 2993 720.2 0 2993 162 9441 720.2 0 9441 163 178 720.2 0 178 164 146 720.2 0 146 165 90 720.2 0 90 166 138 720.2 0 138 167 71 720.2 0 71 168 91 720.2 0 91 169 83 720.2 0 83 170 92 720.2 0 92 171 129 720.2 0 129 172 57 720.2 0 57 173 112 720.2 0 112 174 107 720.2 0 107 175 428 720.2 0 428 176 501 720.2 0 501 177 559 720.2 0 559 178 117 720.2 0 117 179 189 720.2 0 189 180 1062 720.2 0 1062 181 1667 720.2 0 1667 182 2222 720.2 0 2222 183 1266 720.2 0 1266 184 82 720.2 0 82 185 109 720.2 0 109 186 112 720.2 0 112 187 85 720.2 0 85 188 83 720.2 0 83 189 63 720.2 0 63 190 64 720.2 0 64 191 64 720.2 0 64 192 66 720.2 0 66 193 51 720.2 0 51 194 31 720.2 0 31 195 53 720.2 0 53 196 51 720.2 0 51 197 45 720.2 0 45 198 51 720.2 0 51 199 109 720.2 0 109 200 69 720.2 0 69 201 64 720.2 0 64 202 53 720.2 0 53 203 71 720.2 0 71 204 57 720.2 0 57 205 56 720.2 0 56 206 51 720.2 0 51 207 54 720.2 0 54 208 78 720.2 0 78 209 87 720.2 0 87 210 140 720.2 0 140 211 148 720.2 0 148 212 1272 720.2 0 1272 213 615 720.2 0 615 214 1801 720.2 0 1801 215 112 720.2 0 112 216 76 720.2 0 76 217 86 720.2 0 86 218 133 720.2 0 133 219 152 720.2 0 152 220 106 720.2 0 106 221 117 720.2 0 117 222 72 720.2 0 72 223 92 720.2 0 92 224 170 720.2 0 170 225 211 720.2 0 211 226 184 720.2 0 184 227 74 720.2 0 74 228 74 720.2 0 74 229 88 720.2 0 88 230 54 720.2 0 54 231 65 720.2 0 65 232 53 720.2 0 53 233 88 720.2 0 88 234 92 720.2 0 92 235 117 720.2 0 117 236 80 720.2 0 80 237 105 720.2 0 105 238 157 720.2 0 157 239 193 720.2 0 193 240 100 720.2 0 100 241 160 720.2 0 160 242 110 720.2 0 110 243 170 720.2 0 170 244 170 720.2 0 170 245 199 720.2 0 199 246 196 720.2 0 196 247 180 720.2 0 180 248 254 720.2 0 254 249 133 720.2 0 133 250 77 720.2 0 77 251 102 720.2 0 102 252 120 720.2 0 120 253 109 720.2 0 109 254 158 720.2 0 158 255 307 720.2 0 307 256 870 720.2 0 870 257 955 720.2 0 955 258 1206 720.2 0 1206 259 259 720.2 0 259 260 165 720.2 0 165 261 114 720.2 0 114 262 135 720.2 0 135 263 123 720.2 0 123 264 121 720.2 0 121 265 105 720.2 0 105 266 383 720.2 0 383 267 618 720.2 0 618 268 1018 720.2 0 1018 269 302 720.2 0 302 270 146 720.2 0 146 271 177 720.2 0 177 272 394 720.2 0 394 273 1267 720.2 0 1267 274 1099 720.2 0 1099 275 1355 720.2 0 1355 276 332 720.2 0 332 277 318 720.2 0 318 278 265 720.2 0 265 279 191 720.2 0 191 280 322 720.2 0 322 281 355 720.2 0 355 282 274 720.2 0 274 283 166 720.2 0 166 284 191 720.2 0 191 285 186 720.2 0 186 286 218 720.2 0 218 287 361 720.2 0 361 288 465 720.2 0 465 289 471 720.2 0 471 290 110 720.2 0 110 291 117 720.2 0 117 292 193 720.2 0 193 293 295 720.2 0 295 294 216 720.2 0 216 295 174 720.2 0 174 296 98 720.2 0 98 297 92 720.2 0 92 298 138 720.2 0 138 299 115 720.2 0 115 300 118 720.2 0 118 301 276 720.2 0 276 Finished in 2.67 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 54,130 Reads with adapters: 302 (0.6%) Reads that were too short: 102 (0.2%) Reads written (passing filters): 200 (0.4%) Total basepairs processed: 8,619,303 bp Total written (filtered): 43,871 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 302 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 17 119 0.0 3 114 5 18 4 0.0 3 0 2 0 2 19 2 0.0 3 0 0 0 2 20 1 0.0 3 1 28 1 0.0 3 1 33 4 0.0 3 0 0 0 4 34 3 0.0 3 0 0 0 3 35 5 0.0 3 0 0 0 5 36 1 0.0 3 0 0 0 1 37 6 0.0 3 0 0 0 6 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 8 0.0 3 0 0 0 8 49 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 54 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 6 0.0 3 0 0 3 3 61 1 0.0 3 0 0 0 1 62 11 0.0 3 0 0 0 11 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 6 0.0 3 0 1 0 5 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 1 0 1 84 4 0.0 3 0 0 0 4 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 1 99 3 0.0 3 0 1 2 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 7 0.0 3 0 0 0 7 114 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 5 0.0 3 0 0 0 5 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 127 3 0.0 3 0 0 0 3 128 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 165 4 0.0 3 0 0 1 3 166 4 0.0 3 0 0 0 4 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 204 1 0.0 3 0 1 205 1 0.0 3 0 0 1 214 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 200 Reads with adapters: 8 (4.0%) Reads written (passing filters): 200 (100.0%) Total basepairs processed: 43,871 bp Total written (filtered): 43,845 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 8 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 12.5% G: 37.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 3.1 0 6 4 2 0.8 0 2