This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.56 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 82,197 (3.7%) Reads that were too short: 12,681 (0.6%) Reads written (passing filters): 69,516 (3.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 16,382,458 bp (2.4%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 82197 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 188 546.6 0 188 7 318 546.6 0 318 8 15197 546.6 0 15197 9 13237 546.6 0 13237 10 44 546.6 0 44 11 34 546.6 0 34 12 33 546.6 0 33 13 71 546.6 0 71 14 14 546.6 0 14 15 25 546.6 0 25 16 21 546.6 0 21 17 28 546.6 0 28 18 29 546.6 0 29 19 22 546.6 0 22 20 19 546.6 0 19 21 18 546.6 0 18 22 20 546.6 0 20 23 23 546.6 0 23 24 21 546.6 0 21 25 30 546.6 0 30 26 25 546.6 0 25 27 36 546.6 0 36 28 23 546.6 0 23 29 32 546.6 0 32 30 32 546.6 0 32 31 170 546.6 0 170 32 137 546.6 0 137 33 136 546.6 0 136 34 32 546.6 0 32 35 45 546.6 0 45 36 20 546.6 0 20 37 46 546.6 0 46 38 44 546.6 0 44 39 59 546.6 0 59 40 31 546.6 0 31 41 33 546.6 0 33 42 43 546.6 0 43 43 40 546.6 0 40 44 24 546.6 0 24 45 22 546.6 0 22 46 36 546.6 0 36 47 44 546.6 0 44 48 52 546.6 0 52 49 60 546.6 0 60 50 41 546.6 0 41 51 110 546.6 0 110 52 35 546.6 0 35 53 55 546.6 0 55 54 38 546.6 0 38 55 42 546.6 0 42 56 58 546.6 0 58 57 43 546.6 0 43 58 46 546.6 0 46 59 44 546.6 0 44 60 26 546.6 0 26 61 28 546.6 0 28 62 32 546.6 0 32 63 32 546.6 0 32 64 38 546.6 0 38 65 35 546.6 0 35 66 55 546.6 0 55 67 54 546.6 0 54 68 52 546.6 0 52 69 44 546.6 0 44 70 34 546.6 0 34 71 38 546.6 0 38 72 45 546.6 0 45 73 40 546.6 0 40 74 41 546.6 0 41 75 43 546.6 0 43 76 79 546.6 0 79 77 53 546.6 0 53 78 42 546.6 0 42 79 36 546.6 0 36 80 54 546.6 0 54 81 34 546.6 0 34 82 59 546.6 0 59 83 47 546.6 0 47 84 58 546.6 0 58 85 120 546.6 0 120 86 868 546.6 0 868 87 185 546.6 0 185 88 383 546.6 0 383 89 92 546.6 0 92 90 214 546.6 0 214 91 80 546.6 0 80 92 52 546.6 0 52 93 60 546.6 0 60 94 60 546.6 0 60 95 59 546.6 0 59 96 90 546.6 0 90 97 55 546.6 0 55 98 115 546.6 0 115 99 151 546.6 0 151 100 214 546.6 0 214 101 399 546.6 0 399 102 1048 546.6 0 1048 103 17900 546.6 0 17900 104 2170 546.6 0 2170 105 5685 546.6 0 5685 106 468 546.6 0 468 107 497 546.6 0 497 108 372 546.6 0 372 109 41 546.6 0 41 110 54 546.6 0 54 111 44 546.6 0 44 112 94 546.6 0 94 113 59 546.6 0 59 114 29 546.6 0 29 115 15 546.6 0 15 116 21 546.6 0 21 117 32 546.6 0 32 118 48 546.6 0 48 119 44 546.6 0 44 120 39 546.6 0 39 121 30 546.6 0 30 122 31 546.6 0 31 123 39 546.6 0 39 124 44 546.6 0 44 125 47 546.6 0 47 126 48 546.6 0 48 127 55 546.6 0 55 128 40 546.6 0 40 129 54 546.6 0 54 130 30 546.6 0 30 131 52 546.6 0 52 132 53 546.6 0 53 133 45 546.6 0 45 134 45 546.6 0 45 135 64 546.6 0 64 136 37 546.6 0 37 137 37 546.6 0 37 138 58 546.6 0 58 139 127 546.6 0 127 140 114 546.6 0 114 141 72 546.6 0 72 142 57 546.6 0 57 143 262 546.6 0 262 144 74 546.6 0 74 145 188 546.6 0 188 146 49 546.6 0 49 147 41 546.6 0 41 148 55 546.6 0 55 149 129 546.6 0 129 150 244 546.6 0 244 151 164 546.6 0 164 152 114 546.6 0 114 153 115 546.6 0 115 154 68 546.6 0 68 155 81 546.6 0 81 156 61 546.6 0 61 157 56 546.6 0 56 158 69 546.6 0 69 159 75 546.6 0 75 160 89 546.6 0 89 161 58 546.6 0 58 162 33 546.6 0 33 163 26 546.6 0 26 164 43 546.6 0 43 165 48 546.6 0 48 166 72 546.6 0 72 167 45 546.6 0 45 168 29 546.6 0 29 169 33 546.6 0 33 170 38 546.6 0 38 171 50 546.6 0 50 172 62 546.6 0 62 173 66 546.6 0 66 174 25 546.6 0 25 175 38 546.6 0 38 176 39 546.6 0 39 177 33 546.6 0 33 178 102 546.6 0 102 179 92 546.6 0 92 180 158 546.6 0 158 181 72 546.6 0 72 182 47 546.6 0 47 183 53 546.6 0 53 184 54 546.6 0 54 185 126 546.6 0 126 186 60 546.6 0 60 187 53 546.6 0 53 188 42 546.6 0 42 189 89 546.6 0 89 190 55 546.6 0 55 191 123 546.6 0 123 192 40 546.6 0 40 193 53 546.6 0 53 194 25 546.6 0 25 195 49 546.6 0 49 196 41 546.6 0 41 197 53 546.6 0 53 198 37 546.6 0 37 199 46 546.6 0 46 200 59 546.6 0 59 201 54 546.6 0 54 202 49 546.6 0 49 203 43 546.6 0 43 204 58 546.6 0 58 205 129 546.6 0 129 206 172 546.6 0 172 207 156 546.6 0 156 208 101 546.6 0 101 209 65 546.6 0 65 210 63 546.6 0 63 211 128 546.6 0 128 212 73 546.6 0 73 213 104 546.6 0 104 214 120 546.6 0 120 215 99 546.6 0 99 216 106 546.6 0 106 217 97 546.6 0 97 218 88 546.6 0 88 219 107 546.6 0 107 220 89 546.6 0 89 221 152 546.6 0 152 222 61 546.6 0 61 223 97 546.6 0 97 224 89 546.6 0 89 225 108 546.6 0 108 226 66 546.6 0 66 227 70 546.6 0 70 228 40 546.6 0 40 229 61 546.6 0 61 230 53 546.6 0 53 231 70 546.6 0 70 232 53 546.6 0 53 233 84 546.6 0 84 234 147 546.6 0 147 235 157 546.6 0 157 236 91 546.6 0 91 237 56 546.6 0 56 238 55 546.6 0 55 239 67 546.6 0 67 240 73 546.6 0 73 241 86 546.6 0 86 242 72 546.6 0 72 243 74 546.6 0 74 244 51 546.6 0 51 245 70 546.6 0 70 246 41 546.6 0 41 247 74 546.6 0 74 248 56 546.6 0 56 249 25 546.6 0 25 250 48 546.6 0 48 251 72 546.6 0 72 252 76 546.6 0 76 253 50 546.6 0 50 254 67 546.6 0 67 255 68 546.6 0 68 256 91 546.6 0 91 257 128 546.6 0 128 258 99 546.6 0 99 259 376 546.6 0 376 260 312 546.6 0 312 261 798 546.6 0 798 262 1238 546.6 0 1238 263 1473 546.6 0 1473 264 79 546.6 0 79 265 66 546.6 0 66 266 51 546.6 0 51 267 91 546.6 0 91 268 60 546.6 0 60 269 27 546.6 0 27 270 45 546.6 0 45 271 55 546.6 0 55 272 76 546.6 0 76 273 66 546.6 0 66 274 149 546.6 0 149 275 69 546.6 0 69 276 76 546.6 0 76 277 69 546.6 0 69 278 88 546.6 0 88 279 183 546.6 0 183 280 273 546.6 0 273 281 272 546.6 0 272 282 246 546.6 0 246 283 104 546.6 0 104 284 191 546.6 0 191 285 55 546.6 0 55 286 64 546.6 0 64 287 81 546.6 0 81 288 79 546.6 0 79 289 62 546.6 0 62 290 62 546.6 0 62 291 46 546.6 0 46 292 53 546.6 0 53 293 118 546.6 0 118 294 88 546.6 0 88 295 93 546.6 0 93 296 68 546.6 0 68 297 85 546.6 0 85 298 66 546.6 0 66 299 90 546.6 0 90 300 121 546.6 0 121 301 172 546.6 0 172 Finished in 2.92 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 69,516 Reads with adapters: 15,254 (21.9%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 15,190 (21.9%) Total basepairs processed: 16,382,458 bp Total written (filtered): 4,187,419 bp (25.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15254 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 3 0.0 3 0 0 1 2 16 139 0.0 3 0 109 20 10 17 14864 0.0 3 13929 771 124 40 18 47 0.0 3 3 27 9 8 19 47 0.0 3 0 0 26 21 20 52 0.0 3 0 1 0 51 21 2 0.0 3 1 0 0 1 26 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 1 65 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 67 4 0.0 3 0 0 0 4 68 2 0.0 3 0 0 0 2 69 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 15,190 Reads with adapters: 58 (0.4%) Reads written (passing filters): 15,190 (100.0%) Total basepairs processed: 4,187,419 bp Total written (filtered): 4,187,229 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 58 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.3% C: 25.9% G: 29.3% T: 15.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 237.3 0 47 4 6 59.3 0 6 5 5 14.8 0 5