This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.66 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 76,696 (3.4%) Reads that were too short: 7,815 (0.3%) Reads written (passing filters): 68,881 (3.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 16,348,635 bp (2.4%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 76696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 153 546.6 0 153 7 289 546.6 0 289 8 13669 546.6 0 13669 9 15724 546.6 0 15724 10 33 546.6 0 33 11 18 546.6 0 18 12 18 546.6 0 18 13 16 546.6 0 16 14 23 546.6 0 23 15 21 546.6 0 21 16 22 546.6 0 22 17 18 546.6 0 18 18 27 546.6 0 27 19 11 546.6 0 11 20 14 546.6 0 14 21 17 546.6 0 17 22 25 546.6 0 25 23 21 546.6 0 21 24 9 546.6 0 9 25 17 546.6 0 17 26 26 546.6 0 26 27 14 546.6 0 14 28 16 546.6 0 16 29 26 546.6 0 26 30 19 546.6 0 19 31 18 546.6 0 18 32 21 546.6 0 21 33 29 546.6 0 29 34 17 546.6 0 17 35 20 546.6 0 20 36 14 546.6 0 14 37 20 546.6 0 20 38 20 546.6 0 20 39 33 546.6 0 33 40 14 546.6 0 14 41 20 546.6 0 20 42 29 546.6 0 29 43 27 546.6 0 27 44 16 546.6 0 16 45 19 546.6 0 19 46 24 546.6 0 24 47 26 546.6 0 26 48 28 546.6 0 28 49 22 546.6 0 22 50 32 546.6 0 32 51 123 546.6 0 123 52 27 546.6 0 27 53 32 546.6 0 32 54 19 546.6 0 19 55 22 546.6 0 22 56 15 546.6 0 15 57 32 546.6 0 32 58 27 546.6 0 27 59 33 546.6 0 33 60 23 546.6 0 23 61 24 546.6 0 24 62 27 546.6 0 27 63 20 546.6 0 20 64 18 546.6 0 18 65 18 546.6 0 18 66 33 546.6 0 33 67 30 546.6 0 30 68 25 546.6 0 25 69 34 546.6 0 34 70 27 546.6 0 27 71 26 546.6 0 26 72 26 546.6 0 26 73 38 546.6 0 38 74 26 546.6 0 26 75 15 546.6 0 15 76 50 546.6 0 50 77 20 546.6 0 20 78 17 546.6 0 17 79 22 546.6 0 22 80 22 546.6 0 22 81 21 546.6 0 21 82 25 546.6 0 25 83 37 546.6 0 37 84 32 546.6 0 32 85 83 546.6 0 83 86 444 546.6 0 444 87 149 546.6 0 149 88 279 546.6 0 279 89 54 546.6 0 54 90 126 546.6 0 126 91 83 546.6 0 83 92 52 546.6 0 52 93 43 546.6 0 43 94 43 546.6 0 43 95 36 546.6 0 36 96 66 546.6 0 66 97 56 546.6 0 56 98 87 546.6 0 87 99 153 546.6 0 153 100 160 546.6 0 160 101 359 546.6 0 359 102 906 546.6 0 906 103 20221 546.6 0 20221 104 2610 546.6 0 2610 105 6594 546.6 0 6594 106 278 546.6 0 278 107 222 546.6 0 222 108 157 546.6 0 157 109 40 546.6 0 40 110 22 546.6 0 22 111 30 546.6 0 30 112 29 546.6 0 29 113 26 546.6 0 26 114 25 546.6 0 25 115 17 546.6 0 17 116 19 546.6 0 19 117 14 546.6 0 14 118 27 546.6 0 27 119 26 546.6 0 26 120 27 546.6 0 27 121 22 546.6 0 22 122 13 546.6 0 13 123 18 546.6 0 18 124 21 546.6 0 21 125 22 546.6 0 22 126 35 546.6 0 35 127 37 546.6 0 37 128 21 546.6 0 21 129 27 546.6 0 27 130 19 546.6 0 19 131 28 546.6 0 28 132 33 546.6 0 33 133 42 546.6 0 42 134 46 546.6 0 46 135 41 546.6 0 41 136 19 546.6 0 19 137 13 546.6 0 13 138 20 546.6 0 20 139 81 546.6 0 81 140 30 546.6 0 30 141 28 546.6 0 28 142 23 546.6 0 23 143 227 546.6 0 227 144 36 546.6 0 36 145 98 546.6 0 98 146 24 546.6 0 24 147 22 546.6 0 22 148 28 546.6 0 28 149 97 546.6 0 97 150 191 546.6 0 191 151 132 546.6 0 132 152 115 546.6 0 115 153 114 546.6 0 114 154 52 546.6 0 52 155 78 546.6 0 78 156 37 546.6 0 37 157 34 546.6 0 34 158 55 546.6 0 55 159 46 546.6 0 46 160 51 546.6 0 51 161 24 546.6 0 24 162 28 546.6 0 28 163 15 546.6 0 15 164 27 546.6 0 27 165 27 546.6 0 27 166 30 546.6 0 30 167 22 546.6 0 22 168 23 546.6 0 23 169 23 546.6 0 23 170 35 546.6 0 35 171 37 546.6 0 37 172 36 546.6 0 36 173 53 546.6 0 53 174 38 546.6 0 38 175 20 546.6 0 20 176 20 546.6 0 20 177 15 546.6 0 15 178 80 546.6 0 80 179 53 546.6 0 53 180 145 546.6 0 145 181 47 546.6 0 47 182 28 546.6 0 28 183 34 546.6 0 34 184 46 546.6 0 46 185 58 546.6 0 58 186 26 546.6 0 26 187 30 546.6 0 30 188 27 546.6 0 27 189 37 546.6 0 37 190 44 546.6 0 44 191 133 546.6 0 133 192 26 546.6 0 26 193 26 546.6 0 26 194 20 546.6 0 20 195 29 546.6 0 29 196 19 546.6 0 19 197 51 546.6 0 51 198 23 546.6 0 23 199 36 546.6 0 36 200 38 546.6 0 38 201 32 546.6 0 32 202 40 546.6 0 40 203 23 546.6 0 23 204 36 546.6 0 36 205 93 546.6 0 93 206 122 546.6 0 122 207 94 546.6 0 94 208 50 546.6 0 50 209 28 546.6 0 28 210 44 546.6 0 44 211 65 546.6 0 65 212 40 546.6 0 40 213 51 546.6 0 51 214 57 546.6 0 57 215 45 546.6 0 45 216 44 546.6 0 44 217 33 546.6 0 33 218 32 546.6 0 32 219 53 546.6 0 53 220 50 546.6 0 50 221 65 546.6 0 65 222 42 546.6 0 42 223 48 546.6 0 48 224 71 546.6 0 71 225 65 546.6 0 65 226 45 546.6 0 45 227 35 546.6 0 35 228 23 546.6 0 23 229 32 546.6 0 32 230 30 546.6 0 30 231 44 546.6 0 44 232 39 546.6 0 39 233 32 546.6 0 32 234 53 546.6 0 53 235 56 546.6 0 56 236 31 546.6 0 31 237 29 546.6 0 29 238 41 546.6 0 41 239 37 546.6 0 37 240 54 546.6 0 54 241 63 546.6 0 63 242 51 546.6 0 51 243 50 546.6 0 50 244 35 546.6 0 35 245 35 546.6 0 35 246 28 546.6 0 28 247 42 546.6 0 42 248 40 546.6 0 40 249 28 546.6 0 28 250 35 546.6 0 35 251 28 546.6 0 28 252 46 546.6 0 46 253 32 546.6 0 32 254 40 546.6 0 40 255 40 546.6 0 40 256 48 546.6 0 48 257 80 546.6 0 80 258 62 546.6 0 62 259 203 546.6 0 203 260 189 546.6 0 189 261 612 546.6 0 612 262 1219 546.6 0 1219 263 1052 546.6 0 1052 264 39 546.6 0 39 265 25 546.6 0 25 266 22 546.6 0 22 267 44 546.6 0 44 268 33 546.6 0 33 269 25 546.6 0 25 270 30 546.6 0 30 271 20 546.6 0 20 272 32 546.6 0 32 273 25 546.6 0 25 274 77 546.6 0 77 275 45 546.6 0 45 276 37 546.6 0 37 277 29 546.6 0 29 278 45 546.6 0 45 279 100 546.6 0 100 280 119 546.6 0 119 281 124 546.6 0 124 282 77 546.6 0 77 283 62 546.6 0 62 284 99 546.6 0 99 285 37 546.6 0 37 286 36 546.6 0 36 287 44 546.6 0 44 288 54 546.6 0 54 289 35 546.6 0 35 290 29 546.6 0 29 291 17 546.6 0 17 292 27 546.6 0 27 293 77 546.6 0 77 294 43 546.6 0 43 295 66 546.6 0 66 296 57 546.6 0 57 297 29 546.6 0 29 298 40 546.6 0 40 299 53 546.6 0 53 300 51 546.6 0 51 301 81 546.6 0 81 Finished in 3.20 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 68,881 Reads with adapters: 16,317 (23.7%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 16,312 (23.7%) Total basepairs processed: 16,348,635 bp Total written (filtered): 4,411,297 bp (27.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16317 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 5 0.0 3 0 0 4 0 1 19 61 0.0 3 0 60 1 20 15936 0.0 4 15584 327 15 5 5 21 41 0.0 4 6 31 1 0 3 22 2 0.0 4 1 1 23 20 0.0 4 1 0 0 15 4 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 56 1 0.0 4 0 0 0 0 1 70 1 0.0 4 1 87 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 240 0.0 4 0 0 0 0 240 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 16,312 Reads with adapters: 2,566 (15.7%) Reads written (passing filters): 16,312 (100.0%) Total basepairs processed: 4,411,297 bp Total written (filtered): 4,382,309 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2566 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.8% G: 94.1% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 254.9 0 5 4 7 63.7 0 7 5 1 15.9 0 1 6 1 4.0 0 1 8 1 0.2 0 1 9 1282 0.1 0 47 1235 10 1086 0.0 1 39 1047 11 155 0.0 1 0 155 12 2 0.0 1 0 2 13 3 0.0 1 0 3 14 1 0.0 1 0 1 150 1 0.0 1 0 1 151 1 0.0 1 0 1 208 1 0.0 1 1 223 19 0.0 1 18 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.