This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.09 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 143,394 (6.4%) Reads that were too short: 45,019 (2.0%) Reads written (passing filters): 98,375 (4.4%) Total basepairs processed: 673,916,425 bp Total written (filtered): 22,965,656 bp (3.4%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 143394 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 236 546.6 0 236 7 10565 546.6 0 10565 8 7602 546.6 0 7602 9 40 546.6 0 40 10 46 546.6 0 46 11 46 546.6 0 46 12 16 546.6 0 16 13 36 546.6 0 36 14 23 546.6 0 23 15 20 546.6 0 20 16 47 546.6 0 47 17 27 546.6 0 27 18 44 546.6 0 44 19 61 546.6 0 61 20 16712 546.6 0 16712 21 9111 546.6 0 9111 22 149 546.6 0 149 23 103 546.6 0 103 24 45 546.6 0 45 25 42 546.6 0 42 26 172 546.6 0 172 27 26 546.6 0 26 28 18 546.6 0 18 29 42 546.6 0 42 30 23 546.6 0 23 31 38 546.6 0 38 32 26 546.6 0 26 33 7159 546.6 0 7159 34 767 546.6 0 767 35 38 546.6 0 38 36 37 546.6 0 37 37 19 546.6 0 19 38 53 546.6 0 53 39 57 546.6 0 57 40 39 546.6 0 39 41 39 546.6 0 39 42 35 546.6 0 35 43 49 546.6 0 49 44 53 546.6 0 53 45 42 546.6 0 42 46 48 546.6 0 48 47 51 546.6 0 51 48 89 546.6 0 89 49 74 546.6 0 74 50 85 546.6 0 85 51 24 546.6 0 24 52 44 546.6 0 44 53 44 546.6 0 44 54 41 546.6 0 41 55 50 546.6 0 50 56 59 546.6 0 59 57 52 546.6 0 52 58 55 546.6 0 55 59 49 546.6 0 49 60 64 546.6 0 64 61 65 546.6 0 65 62 61 546.6 0 61 63 92 546.6 0 92 64 61 546.6 0 61 65 96 546.6 0 96 66 85 546.6 0 85 67 98 546.6 0 98 68 87 546.6 0 87 69 208 546.6 0 208 70 133 546.6 0 133 71 164 546.6 0 164 72 108 546.6 0 108 73 128 546.6 0 128 74 177 546.6 0 177 75 458 546.6 0 458 76 670 546.6 0 670 77 3195 546.6 0 3195 78 1146 546.6 0 1146 79 158 546.6 0 158 80 292 546.6 0 292 81 428 546.6 0 428 82 457 546.6 0 457 83 94 546.6 0 94 84 98 546.6 0 98 85 140 546.6 0 140 86 145 546.6 0 145 87 153 546.6 0 153 88 829 546.6 0 829 89 147 546.6 0 147 90 465 546.6 0 465 91 133 546.6 0 133 92 121 546.6 0 121 93 126 546.6 0 126 94 131 546.6 0 131 95 139 546.6 0 139 96 102 546.6 0 102 97 137 546.6 0 137 98 114 546.6 0 114 99 130 546.6 0 130 100 169 546.6 0 169 101 190 546.6 0 190 102 114 546.6 0 114 103 116 546.6 0 116 104 136 546.6 0 136 105 121 546.6 0 121 106 118 546.6 0 118 107 136 546.6 0 136 108 177 546.6 0 177 109 157 546.6 0 157 110 167 546.6 0 167 111 192 546.6 0 192 112 178 546.6 0 178 113 168 546.6 0 168 114 166 546.6 0 166 115 181 546.6 0 181 116 243 546.6 0 243 117 195 546.6 0 195 118 169 546.6 0 169 119 196 546.6 0 196 120 196 546.6 0 196 121 175 546.6 0 175 122 209 546.6 0 209 123 293 546.6 0 293 124 281 546.6 0 281 125 241 546.6 0 241 126 200 546.6 0 200 127 231 546.6 0 231 128 234 546.6 0 234 129 284 546.6 0 284 130 1085 546.6 0 1085 131 2048 546.6 0 2048 132 2999 546.6 0 2999 133 262 546.6 0 262 134 269 546.6 0 269 135 324 546.6 0 324 136 1002 546.6 0 1002 137 1914 546.6 0 1914 138 2213 546.6 0 2213 139 1461 546.6 0 1461 140 513 546.6 0 513 141 280 546.6 0 280 142 182 546.6 0 182 143 161 546.6 0 161 144 135 546.6 0 135 145 157 546.6 0 157 146 183 546.6 0 183 147 182 546.6 0 182 148 242 546.6 0 242 149 192 546.6 0 192 150 165 546.6 0 165 151 200 546.6 0 200 152 257 546.6 0 257 153 220 546.6 0 220 154 178 546.6 0 178 155 148 546.6 0 148 156 135 546.6 0 135 157 139 546.6 0 139 158 181 546.6 0 181 159 130 546.6 0 130 160 130 546.6 0 130 161 189 546.6 0 189 162 230 546.6 0 230 163 188 546.6 0 188 164 224 546.6 0 224 165 210 546.6 0 210 166 228 546.6 0 228 167 140 546.6 0 140 168 148 546.6 0 148 169 125 546.6 0 125 170 259 546.6 0 259 171 1101 546.6 0 1101 172 157 546.6 0 157 173 145 546.6 0 145 174 141 546.6 0 141 175 153 546.6 0 153 176 173 546.6 0 173 177 160 546.6 0 160 178 145 546.6 0 145 179 210 546.6 0 210 180 148 546.6 0 148 181 161 546.6 0 161 182 125 546.6 0 125 183 170 546.6 0 170 184 185 546.6 0 185 185 196 546.6 0 196 186 180 546.6 0 180 187 227 546.6 0 227 188 183 546.6 0 183 189 478 546.6 0 478 190 231 546.6 0 231 191 206 546.6 0 206 192 191 546.6 0 191 193 199 546.6 0 199 194 188 546.6 0 188 195 242 546.6 0 242 196 382 546.6 0 382 197 301 546.6 0 301 198 209 546.6 0 209 199 143 546.6 0 143 200 143 546.6 0 143 201 138 546.6 0 138 202 153 546.6 0 153 203 164 546.6 0 164 204 161 546.6 0 161 205 171 546.6 0 171 206 179 546.6 0 179 207 175 546.6 0 175 208 178 546.6 0 178 209 224 546.6 0 224 210 169 546.6 0 169 211 207 546.6 0 207 212 190 546.6 0 190 213 159 546.6 0 159 214 208 546.6 0 208 215 187 546.6 0 187 216 189 546.6 0 189 217 186 546.6 0 186 218 191 546.6 0 191 219 191 546.6 0 191 220 192 546.6 0 192 221 209 546.6 0 209 222 313 546.6 0 313 223 280 546.6 0 280 224 319 546.6 0 319 225 328 546.6 0 328 226 931 546.6 0 931 227 406 546.6 0 406 228 413 546.6 0 413 229 490 546.6 0 490 230 550 546.6 0 550 231 360 546.6 0 360 232 521 546.6 0 521 233 491 546.6 0 491 234 187 546.6 0 187 235 220 546.6 0 220 236 242 546.6 0 242 237 231 546.6 0 231 238 250 546.6 0 250 239 462 546.6 0 462 240 356 546.6 0 356 241 370 546.6 0 370 242 460 546.6 0 460 243 481 546.6 0 481 244 569 546.6 0 569 245 360 546.6 0 360 246 483 546.6 0 483 247 515 546.6 0 515 248 444 546.6 0 444 249 337 546.6 0 337 250 544 546.6 0 544 251 2470 546.6 0 2470 252 3241 546.6 0 3241 253 8993 546.6 0 8993 254 456 546.6 0 456 255 212 546.6 0 212 256 357 546.6 0 357 257 358 546.6 0 358 258 1033 546.6 0 1033 259 415 546.6 0 415 260 911 546.6 0 911 261 633 546.6 0 633 262 285 546.6 0 285 263 221 546.6 0 221 264 318 546.6 0 318 265 294 546.6 0 294 266 205 546.6 0 205 267 206 546.6 0 206 268 170 546.6 0 170 269 214 546.6 0 214 270 238 546.6 0 238 271 275 546.6 0 275 272 251 546.6 0 251 273 247 546.6 0 247 274 241 546.6 0 241 275 188 546.6 0 188 276 348 546.6 0 348 277 233 546.6 0 233 278 286 546.6 0 286 279 190 546.6 0 190 280 199 546.6 0 199 281 192 546.6 0 192 282 170 546.6 0 170 283 213 546.6 0 213 284 221 546.6 0 221 285 278 546.6 0 278 286 280 546.6 0 280 287 239 546.6 0 239 288 209 546.6 0 209 289 216 546.6 0 216 290 237 546.6 0 237 291 217 546.6 0 217 292 278 546.6 0 278 293 197 546.6 0 197 294 184 546.6 0 184 295 210 546.6 0 210 296 220 546.6 0 220 297 765 546.6 0 765 298 345 546.6 0 345 299 599 546.6 0 599 300 221 546.6 0 221 301 244 546.6 0 244 Finished in 3.95 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 98,375 Reads with adapters: 10,724 (10.9%) Reads that were too short: 151 (0.2%) Reads written (passing filters): 10,573 (10.7%) Total basepairs processed: 22,965,656 bp Total written (filtered): 2,890,820 bp (12.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10724 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 29 0.0 3 0 0 8 21 16 106 0.0 3 0 77 9 20 17 10040 0.0 3 9109 720 158 53 18 53 0.0 3 20 20 6 7 19 71 0.0 3 1 4 36 30 20 28 0.0 3 0 0 0 28 27 3 0.0 3 0 0 0 3 29 4 0.0 3 0 0 0 4 30 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 0 3 35 5 0.0 3 0 0 0 5 36 3 0.0 3 0 0 0 3 40 6 0.0 3 0 0 0 6 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 53 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 95 0.0 3 0 0 2 93 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 17 0.0 3 0 0 0 17 65 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 1 2 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 3 0.0 3 0 0 0 3 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 12 0.0 3 0 0 1 11 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 3 0.0 3 0 0 0 3 111 5 0.0 3 0 0 0 5 112 8 0.0 3 0 0 0 8 114 4 0.0 3 0 0 0 4 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 1 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 145 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 1 1 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 9 0.0 3 0 0 0 9 163 4 0.0 3 0 0 0 4 164 4 0.0 3 0 0 1 3 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 170 3 0.0 3 0 0 0 3 171 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 174 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 6 0.0 3 0 0 0 6 179 2 0.0 3 0 0 0 2 180 2 0.0 3 0 0 0 2 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 1 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 210 3 0.0 3 0 0 0 3 211 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 220 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 249 2 0.0 3 0 0 0 2 254 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 294 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 10,573 Reads with adapters: 408 (3.9%) Reads written (passing filters): 10,573 (100.0%) Total basepairs processed: 2,890,820 bp Total written (filtered): 2,889,590 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 408 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.5% C: 79.2% G: 7.1% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 405 165.2 0 405 4 1 41.3 0 1 5 1 10.3 0 1 6 1 2.6 0 1