This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.35 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 76,098 (3.4%) Reads that were too short: 31,634 (1.4%) Reads written (passing filters): 44,464 (2.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 10,296,874 bp (1.5%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 76098 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 271 546.6 0 271 7 8134 546.6 0 8134 8 11177 546.6 0 11177 9 57 546.6 0 57 10 45 546.6 0 45 11 52 546.6 0 52 12 27 546.6 0 27 13 39 546.6 0 39 14 21 546.6 0 21 15 28 546.6 0 28 16 24 546.6 0 24 17 31 546.6 0 31 18 32 546.6 0 32 19 31 546.6 0 31 20 21 546.6 0 21 21 46 546.6 0 46 22 56 546.6 0 56 23 69 546.6 0 69 24 47 546.6 0 47 25 31 546.6 0 31 26 27 546.6 0 27 27 30 546.6 0 30 28 34 546.6 0 34 29 24 546.6 0 24 30 36 546.6 0 36 31 33 546.6 0 33 32 46 546.6 0 46 33 63 546.6 0 63 34 34 546.6 0 34 35 37 546.6 0 37 36 42 546.6 0 42 37 38 546.6 0 38 38 62 546.6 0 62 39 46 546.6 0 46 40 38 546.6 0 38 41 31 546.6 0 31 42 30 546.6 0 30 43 39 546.6 0 39 44 66 546.6 0 66 45 42 546.6 0 42 46 47 546.6 0 47 47 55 546.6 0 55 48 133 546.6 0 133 49 147 546.6 0 147 50 168 546.6 0 168 51 38 546.6 0 38 52 35 546.6 0 35 53 39 546.6 0 39 54 31 546.6 0 31 55 39 546.6 0 39 56 45 546.6 0 45 57 43 546.6 0 43 58 32 546.6 0 32 59 30 546.6 0 30 60 44 546.6 0 44 61 33 546.6 0 33 62 34 546.6 0 34 63 64 546.6 0 64 64 35 546.6 0 35 65 46 546.6 0 46 66 49 546.6 0 49 67 37 546.6 0 37 68 43 546.6 0 43 69 147 546.6 0 147 70 57 546.6 0 57 71 100 546.6 0 100 72 61 546.6 0 61 73 55 546.6 0 55 74 65 546.6 0 65 75 405 546.6 0 405 76 465 546.6 0 465 77 1994 546.6 0 1994 78 1021 546.6 0 1021 79 63 546.6 0 63 80 87 546.6 0 87 81 141 546.6 0 141 82 156 546.6 0 156 83 36 546.6 0 36 84 39 546.6 0 39 85 53 546.6 0 53 86 41 546.6 0 41 87 40 546.6 0 40 88 39 546.6 0 39 89 40 546.6 0 40 90 43 546.6 0 43 91 47 546.6 0 47 92 52 546.6 0 52 93 26 546.6 0 26 94 34 546.6 0 34 95 50 546.6 0 50 96 38 546.6 0 38 97 43 546.6 0 43 98 48 546.6 0 48 99 49 546.6 0 49 100 52 546.6 0 52 101 53 546.6 0 53 102 59 546.6 0 59 103 62 546.6 0 62 104 66 546.6 0 66 105 59 546.6 0 59 106 44 546.6 0 44 107 76 546.6 0 76 108 46 546.6 0 46 109 43 546.6 0 43 110 55 546.6 0 55 111 61 546.6 0 61 112 81 546.6 0 81 113 60 546.6 0 60 114 45 546.6 0 45 115 60 546.6 0 60 116 50 546.6 0 50 117 60 546.6 0 60 118 83 546.6 0 83 119 84 546.6 0 84 120 81 546.6 0 81 121 67 546.6 0 67 122 85 546.6 0 85 123 97 546.6 0 97 124 59 546.6 0 59 125 59 546.6 0 59 126 78 546.6 0 78 127 70 546.6 0 70 128 62 546.6 0 62 129 84 546.6 0 84 130 535 546.6 0 535 131 1502 546.6 0 1502 132 1964 546.6 0 1964 133 94 546.6 0 94 134 104 546.6 0 104 135 94 546.6 0 94 136 731 546.6 0 731 137 1868 546.6 0 1868 138 1757 546.6 0 1757 139 773 546.6 0 773 140 52 546.6 0 52 141 43 546.6 0 43 142 60 546.6 0 60 143 45 546.6 0 45 144 34 546.6 0 34 145 41 546.6 0 41 146 44 546.6 0 44 147 73 546.6 0 73 148 131 546.6 0 131 149 57 546.6 0 57 150 36 546.6 0 36 151 35 546.6 0 35 152 30 546.6 0 30 153 67 546.6 0 67 154 31 546.6 0 31 155 41 546.6 0 41 156 43 546.6 0 43 157 46 546.6 0 46 158 41 546.6 0 41 159 38 546.6 0 38 160 47 546.6 0 47 161 116 546.6 0 116 162 120 546.6 0 120 163 97 546.6 0 97 164 115 546.6 0 115 165 131 546.6 0 131 166 58 546.6 0 58 167 90 546.6 0 90 168 66 546.6 0 66 169 62 546.6 0 62 170 169 546.6 0 169 171 1442 546.6 0 1442 172 71 546.6 0 71 173 50 546.6 0 50 174 36 546.6 0 36 175 46 546.6 0 46 176 46 546.6 0 46 177 45 546.6 0 45 178 22 546.6 0 22 179 26 546.6 0 26 180 31 546.6 0 31 181 34 546.6 0 34 182 32 546.6 0 32 183 44 546.6 0 44 184 61 546.6 0 61 185 47 546.6 0 47 186 45 546.6 0 45 187 79 546.6 0 79 188 59 546.6 0 59 189 377 546.6 0 377 190 150 546.6 0 150 191 62 546.6 0 62 192 29 546.6 0 29 193 35 546.6 0 35 194 31 546.6 0 31 195 52 546.6 0 52 196 70 546.6 0 70 197 38 546.6 0 38 198 34 546.6 0 34 199 30 546.6 0 30 200 31 546.6 0 31 201 48 546.6 0 48 202 45 546.6 0 45 203 36 546.6 0 36 204 35 546.6 0 35 205 36 546.6 0 36 206 23 546.6 0 23 207 39 546.6 0 39 208 39 546.6 0 39 209 54 546.6 0 54 210 32 546.6 0 32 211 42 546.6 0 42 212 33 546.6 0 33 213 44 546.6 0 44 214 34 546.6 0 34 215 29 546.6 0 29 216 35 546.6 0 35 217 35 546.6 0 35 218 38 546.6 0 38 219 43 546.6 0 43 220 47 546.6 0 47 221 49 546.6 0 49 222 166 546.6 0 166 223 133 546.6 0 133 224 162 546.6 0 162 225 60 546.6 0 60 226 152 546.6 0 152 227 259 546.6 0 259 228 291 546.6 0 291 229 360 546.6 0 360 230 311 546.6 0 311 231 256 546.6 0 256 232 266 546.6 0 266 233 173 546.6 0 173 234 68 546.6 0 68 235 86 546.6 0 86 236 99 546.6 0 99 237 95 546.6 0 95 238 153 546.6 0 153 239 109 546.6 0 109 240 282 546.6 0 282 241 187 546.6 0 187 242 172 546.6 0 172 243 177 546.6 0 177 244 235 546.6 0 235 245 172 546.6 0 172 246 326 546.6 0 326 247 346 546.6 0 346 248 400 546.6 0 400 249 218 546.6 0 218 250 463 546.6 0 463 251 2877 546.6 0 2877 252 4089 546.6 0 4089 253 11791 546.6 0 11791 254 394 546.6 0 394 255 85 546.6 0 85 256 93 546.6 0 93 257 88 546.6 0 88 258 231 546.6 0 231 259 80 546.6 0 80 260 175 546.6 0 175 261 170 546.6 0 170 262 102 546.6 0 102 263 91 546.6 0 91 264 106 546.6 0 106 265 84 546.6 0 84 266 77 546.6 0 77 267 74 546.6 0 74 268 66 546.6 0 66 269 73 546.6 0 73 270 76 546.6 0 76 271 90 546.6 0 90 272 89 546.6 0 89 273 78 546.6 0 78 274 53 546.6 0 53 275 54 546.6 0 54 276 70 546.6 0 70 277 95 546.6 0 95 278 71 546.6 0 71 279 56 546.6 0 56 280 56 546.6 0 56 281 74 546.6 0 74 282 68 546.6 0 68 283 91 546.6 0 91 284 114 546.6 0 114 285 168 546.6 0 168 286 190 546.6 0 190 287 138 546.6 0 138 288 98 546.6 0 98 289 125 546.6 0 125 290 128 546.6 0 128 291 120 546.6 0 120 292 125 546.6 0 125 293 94 546.6 0 94 294 100 546.6 0 100 295 156 546.6 0 156 296 103 546.6 0 103 297 536 546.6 0 536 298 189 546.6 0 189 299 324 546.6 0 324 300 86 546.6 0 86 301 128 546.6 0 128 Finished in 2.05 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 44,464 Reads with adapters: 11,948 (26.9%) Reads that were too short: 38 (0.1%) Reads written (passing filters): 11,910 (26.8%) Total basepairs processed: 10,296,874 bp Total written (filtered): 3,219,082 bp (31.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11948 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 1 19 64 0.0 3 0 61 3 20 11495 0.0 4 11314 177 3 1 21 24 0.0 4 6 17 1 22 4 0.0 4 1 1 2 23 11 0.0 4 0 0 0 10 1 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 38 1 0.0 4 1 54 6 0.0 4 0 0 0 0 6 62 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 99 3 0.0 4 0 0 0 0 3 100 4 0.0 4 0 0 0 0 4 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 291 0.0 4 0 0 0 0 291 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 152 3 0.0 4 3 153 1 0.0 4 0 1 154 7 0.0 4 3 2 1 1 155 5 0.0 4 4 0 0 1 156 2 0.0 4 1 0 1 159 4 0.0 4 0 0 0 0 4 160 2 0.0 4 1 0 0 1 161 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 11,910 Reads with adapters: 1,105 (9.3%) Reads written (passing filters): 11,910 (100.0%) Total basepairs processed: 3,219,082 bp Total written (filtered): 3,206,052 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1105 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 3.0% G: 93.7% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 186.1 0 5 4 3 46.5 0 3 9 16 0.0 0 0 16 10 287 0.0 1 9 278 11 785 0.0 1 4 781 12 2 0.0 1 0 2 151 1 0.0 1 0 1 190 3 0.0 1 2 1 191 1 0.0 1 1 209 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.