This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 347,477 (15.5%) Reads that were too short: 273,692 (12.2%) Reads written (passing filters): 73,785 (3.3%) Total basepairs processed: 673,916,425 bp Total written (filtered): 18,617,471 bp (2.8%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 347477 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1230 546.6 0 1230 7 23268 546.6 0 23268 8 977 546.6 0 977 9 27590 546.6 0 27590 10 67 546.6 0 67 11 41 546.6 0 41 12 26 546.6 0 26 13 33 546.6 0 33 14 20 546.6 0 20 15 25 546.6 0 25 16 18 546.6 0 18 17 26 546.6 0 26 18 37 546.6 0 37 19 35 546.6 0 35 20 29 546.6 0 29 21 42 546.6 0 42 22 27 546.6 0 27 23 26 546.6 0 26 24 12 546.6 0 12 25 16 546.6 0 16 26 12 546.6 0 12 27 10 546.6 0 10 28 20 546.6 0 20 29 20 546.6 0 20 30 11 546.6 0 11 31 22 546.6 0 22 32 23 546.6 0 23 33 19 546.6 0 19 34 18 546.6 0 18 35 20 546.6 0 20 36 28 546.6 0 28 37 15 546.6 0 15 38 22 546.6 0 22 39 18 546.6 0 18 40 22 546.6 0 22 41 24 546.6 0 24 42 22 546.6 0 22 43 25 546.6 0 25 44 25 546.6 0 25 45 35 546.6 0 35 46 52 546.6 0 52 47 201 546.6 0 201 48 89 546.6 0 89 49 55 546.6 0 55 50 20 546.6 0 20 51 28 546.6 0 28 52 23 546.6 0 23 53 41 546.6 0 41 54 48 546.6 0 48 55 93 546.6 0 93 56 28 546.6 0 28 57 19 546.6 0 19 58 30 546.6 0 30 59 36 546.6 0 36 60 15 546.6 0 15 61 25 546.6 0 25 62 29 546.6 0 29 63 51 546.6 0 51 64 24 546.6 0 24 65 19 546.6 0 19 66 23 546.6 0 23 67 18 546.6 0 18 68 24 546.6 0 24 69 37 546.6 0 37 70 36 546.6 0 36 71 94 546.6 0 94 72 131 546.6 0 131 73 125 546.6 0 125 74 192 546.6 0 192 75 19 546.6 0 19 76 20 546.6 0 20 77 18 546.6 0 18 78 18 546.6 0 18 79 11 546.6 0 11 80 26 546.6 0 26 81 12 546.6 0 12 82 18 546.6 0 18 83 24 546.6 0 24 84 41 546.6 0 41 85 76 546.6 0 76 86 47 546.6 0 47 87 37 546.6 0 37 88 31 546.6 0 31 89 31 546.6 0 31 90 18 546.6 0 18 91 23 546.6 0 23 92 14 546.6 0 14 93 17 546.6 0 17 94 14 546.6 0 14 95 18 546.6 0 18 96 11 546.6 0 11 97 13 546.6 0 13 98 15 546.6 0 15 99 16 546.6 0 16 100 13 546.6 0 13 101 10 546.6 0 10 102 10 546.6 0 10 103 19 546.6 0 19 104 28 546.6 0 28 105 31 546.6 0 31 106 18 546.6 0 18 107 22 546.6 0 22 108 16 546.6 0 16 109 25 546.6 0 25 110 12 546.6 0 12 111 14 546.6 0 14 112 25 546.6 0 25 113 89 546.6 0 89 114 66 546.6 0 66 115 39 546.6 0 39 116 107 546.6 0 107 117 60 546.6 0 60 118 56 546.6 0 56 119 20 546.6 0 20 120 30 546.6 0 30 121 26 546.6 0 26 122 50 546.6 0 50 123 123 546.6 0 123 124 213 546.6 0 213 125 844 546.6 0 844 126 126 546.6 0 126 127 53 546.6 0 53 128 71 546.6 0 71 129 37 546.6 0 37 130 55 546.6 0 55 131 43 546.6 0 43 132 22 546.6 0 22 133 19 546.6 0 19 134 27 546.6 0 27 135 34 546.6 0 34 136 36 546.6 0 36 137 41 546.6 0 41 138 47 546.6 0 47 139 38 546.6 0 38 140 34 546.6 0 34 141 35 546.6 0 35 142 48 546.6 0 48 143 35 546.6 0 35 144 29 546.6 0 29 145 40 546.6 0 40 146 34 546.6 0 34 147 66 546.6 0 66 148 39 546.6 0 39 149 60 546.6 0 60 150 140 546.6 0 140 151 210 546.6 0 210 152 592 546.6 0 592 153 72 546.6 0 72 154 97 546.6 0 97 155 66 546.6 0 66 156 70 546.6 0 70 157 44 546.6 0 44 158 62 546.6 0 62 159 69 546.6 0 69 160 248 546.6 0 248 161 294 546.6 0 294 162 289 546.6 0 289 163 89 546.6 0 89 164 131 546.6 0 131 165 448 546.6 0 448 166 474 546.6 0 474 167 699 546.6 0 699 168 259 546.6 0 259 169 300 546.6 0 300 170 259 546.6 0 259 171 101 546.6 0 101 172 144 546.6 0 144 173 86 546.6 0 86 174 86 546.6 0 86 175 85 546.6 0 85 176 110 546.6 0 110 177 120 546.6 0 120 178 110 546.6 0 110 179 97 546.6 0 97 180 150 546.6 0 150 181 132 546.6 0 132 182 109 546.6 0 109 183 149 546.6 0 149 184 138 546.6 0 138 185 1247 546.6 0 1247 186 634 546.6 0 634 187 2205 546.6 0 2205 188 1412 546.6 0 1412 189 538 546.6 0 538 190 59 546.6 0 59 191 67 546.6 0 67 192 86 546.6 0 86 193 84 546.6 0 84 194 126 546.6 0 126 195 114 546.6 0 114 196 196 546.6 0 196 197 339 546.6 0 339 198 184 546.6 0 184 199 259 546.6 0 259 200 401 546.6 0 401 201 120 546.6 0 120 202 64 546.6 0 64 203 101 546.6 0 101 204 88 546.6 0 88 205 85 546.6 0 85 206 88 546.6 0 88 207 121 546.6 0 121 208 62 546.6 0 62 209 85 546.6 0 85 210 376 546.6 0 376 211 79 546.6 0 79 212 76 546.6 0 76 213 102 546.6 0 102 214 99 546.6 0 99 215 91 546.6 0 91 216 112 546.6 0 112 217 241 546.6 0 241 218 662 546.6 0 662 219 699 546.6 0 699 220 1244 546.6 0 1244 221 4056 546.6 0 4056 222 8306 546.6 0 8306 223 4122 546.6 0 4122 224 2574 546.6 0 2574 225 450 546.6 0 450 226 475 546.6 0 475 227 718 546.6 0 718 228 291 546.6 0 291 229 392 546.6 0 392 230 411 546.6 0 411 231 382 546.6 0 382 232 599 546.6 0 599 233 956 546.6 0 956 234 1289 546.6 0 1289 235 2869 546.6 0 2869 236 6383 546.6 0 6383 237 90143 546.6 0 90143 238 59116 546.6 0 59116 239 49025 546.6 0 49025 240 2597 546.6 0 2597 241 1012 546.6 0 1012 242 197 546.6 0 197 243 193 546.6 0 193 244 217 546.6 0 217 245 3562 546.6 0 3562 246 1859 546.6 0 1859 247 9300 546.6 0 9300 248 2595 546.6 0 2595 249 2383 546.6 0 2383 250 828 546.6 0 828 251 308 546.6 0 308 252 122 546.6 0 122 253 45 546.6 0 45 254 57 546.6 0 57 255 64 546.6 0 64 256 83 546.6 0 83 257 96 546.6 0 96 258 54 546.6 0 54 259 22 546.6 0 22 260 93 546.6 0 93 261 85 546.6 0 85 262 159 546.6 0 159 263 66 546.6 0 66 264 125 546.6 0 125 265 88 546.6 0 88 266 102 546.6 0 102 267 239 546.6 0 239 268 489 546.6 0 489 269 583 546.6 0 583 270 442 546.6 0 442 271 212 546.6 0 212 272 163 546.6 0 163 273 114 546.6 0 114 274 149 546.6 0 149 275 94 546.6 0 94 276 141 546.6 0 141 277 92 546.6 0 92 278 91 546.6 0 91 279 107 546.6 0 107 280 248 546.6 0 248 281 224 546.6 0 224 282 379 546.6 0 379 283 346 546.6 0 346 284 115 546.6 0 115 285 110 546.6 0 110 286 230 546.6 0 230 287 468 546.6 0 468 288 500 546.6 0 500 289 885 546.6 0 885 290 616 546.6 0 616 291 688 546.6 0 688 292 201 546.6 0 201 293 140 546.6 0 140 294 342 546.6 0 342 295 463 546.6 0 463 296 607 546.6 0 607 297 266 546.6 0 266 298 218 546.6 0 218 299 180 546.6 0 180 300 94 546.6 0 94 301 112 546.6 0 112 Finished in 3.28 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 73,785 Reads with adapters: 29,256 (39.7%) Reads that were too short: 161 (0.2%) Reads written (passing filters): 29,095 (39.4%) Total basepairs processed: 18,617,471 bp Total written (filtered): 7,972,456 bp (42.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 29256 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 10 0.0 3 0 0 6 4 16 155 0.0 3 0 122 20 13 17 28522 0.0 3 26588 1582 288 64 18 56 0.0 3 2 41 5 8 19 51 0.0 3 3 0 31 17 20 92 0.0 3 18 3 1 70 21 58 0.0 3 0 0 0 58 22 1 0.0 3 0 0 0 1 24 10 0.0 3 0 0 0 10 28 1 0.0 3 0 0 0 1 30 3 0.0 3 0 0 0 3 31 2 0.0 3 2 32 35 0.0 3 0 0 0 35 33 2 0.0 3 0 0 0 2 35 3 0.0 3 0 0 0 3 37 4 0.0 3 0 0 0 4 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 2 0.0 3 1 0 0 1 44 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 47 2 0.0 3 0 0 0 2 48 29 0.0 3 0 0 0 29 49 4 0.0 3 0 0 0 4 50 5 0.0 3 0 0 0 5 51 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 10 0.0 3 0 0 0 10 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 66 6 0.0 3 0 0 0 6 67 1 0.0 3 0 0 0 1 68 6 0.0 3 0 0 0 6 72 3 0.0 3 0 0 0 3 74 5 0.0 3 0 0 0 5 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 0 3 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 1 1 106 1 0.0 3 0 0 0 1 107 7 0.0 3 0 0 0 7 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 13 0.0 3 0 0 0 13 119 6 0.0 3 0 0 1 5 120 2 0.0 3 0 0 0 2 121 8 0.0 3 0 0 0 8 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 1 1 128 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 6 0.0 3 0 0 1 5 142 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 160 6 0.0 3 0 0 0 6 161 1 0.0 3 0 0 1 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 1 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 3 0.0 3 0 0 0 3 197 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 221 5 0.0 3 0 0 0 5 232 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 1 2 282 7 0.0 3 0 0 0 7 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.00 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 29,095 Reads with adapters: 778 (2.7%) Reads written (passing filters): 29,095 (100.0%) Total basepairs processed: 7,972,456 bp Total written (filtered): 7,970,077 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 778 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.1% C: 12.7% G: 38.2% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 738 454.6 0 738 4 37 113.7 0 37 5 2 28.4 0 2 7 1 1.8 0 1