This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.95 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 426,683 (19.1%) Reads that were too short: 360,971 (16.1%) Reads written (passing filters): 65,712 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,660,104 bp (2.6%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 426683 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1460 546.6 0 1460 7 29441 546.6 0 29441 8 425 546.6 0 425 9 23251 546.6 0 23251 10 43 546.6 0 43 11 32 546.6 0 32 12 16 546.6 0 16 13 37 546.6 0 37 14 15 546.6 0 15 15 21 546.6 0 21 16 7 546.6 0 7 17 11 546.6 0 11 18 25 546.6 0 25 19 18 546.6 0 18 20 27 546.6 0 27 21 34 546.6 0 34 22 21 546.6 0 21 23 17 546.6 0 17 24 12 546.6 0 12 25 16 546.6 0 16 26 9 546.6 0 9 27 16 546.6 0 16 28 16 546.6 0 16 29 15 546.6 0 15 30 14 546.6 0 14 31 13 546.6 0 13 32 14 546.6 0 14 33 20 546.6 0 20 34 16 546.6 0 16 35 22 546.6 0 22 36 23 546.6 0 23 37 15 546.6 0 15 38 25 546.6 0 25 39 15 546.6 0 15 40 13 546.6 0 13 41 34 546.6 0 34 42 19 546.6 0 19 43 26 546.6 0 26 44 13 546.6 0 13 45 20 546.6 0 20 46 25 546.6 0 25 47 28 546.6 0 28 48 10 546.6 0 10 49 43 546.6 0 43 50 21 546.6 0 21 51 23 546.6 0 23 52 14 546.6 0 14 53 46 546.6 0 46 54 44 546.6 0 44 55 68 546.6 0 68 56 36 546.6 0 36 57 17 546.6 0 17 58 15 546.6 0 15 59 22 546.6 0 22 60 19 546.6 0 19 61 18 546.6 0 18 62 27 546.6 0 27 63 35 546.6 0 35 64 11 546.6 0 11 65 20 546.6 0 20 66 17 546.6 0 17 67 13 546.6 0 13 68 12 546.6 0 12 69 17 546.6 0 17 70 18 546.6 0 18 71 22 546.6 0 22 72 37 546.6 0 37 73 31 546.6 0 31 74 37 546.6 0 37 75 18 546.6 0 18 76 13 546.6 0 13 77 12 546.6 0 12 78 19 546.6 0 19 79 7 546.6 0 7 80 20 546.6 0 20 81 27 546.6 0 27 82 16 546.6 0 16 83 20 546.6 0 20 84 20 546.6 0 20 85 41 546.6 0 41 86 27 546.6 0 27 87 23 546.6 0 23 88 23 546.6 0 23 89 18 546.6 0 18 90 18 546.6 0 18 91 13 546.6 0 13 92 14 546.6 0 14 93 25 546.6 0 25 94 9 546.6 0 9 95 20 546.6 0 20 96 17 546.6 0 17 97 13 546.6 0 13 98 25 546.6 0 25 99 30 546.6 0 30 100 17 546.6 0 17 101 8 546.6 0 8 102 13 546.6 0 13 103 13 546.6 0 13 104 18 546.6 0 18 105 24 546.6 0 24 106 17 546.6 0 17 107 23 546.6 0 23 108 32 546.6 0 32 109 16 546.6 0 16 110 6 546.6 0 6 111 18 546.6 0 18 112 23 546.6 0 23 113 36 546.6 0 36 114 20 546.6 0 20 115 42 546.6 0 42 116 60 546.6 0 60 117 30 546.6 0 30 118 25 546.6 0 25 119 16 546.6 0 16 120 26 546.6 0 26 121 11 546.6 0 11 122 44 546.6 0 44 123 52 546.6 0 52 124 46 546.6 0 46 125 315 546.6 0 315 126 54 546.6 0 54 127 40 546.6 0 40 128 42 546.6 0 42 129 40 546.6 0 40 130 29 546.6 0 29 131 13 546.6 0 13 132 17 546.6 0 17 133 21 546.6 0 21 134 26 546.6 0 26 135 35 546.6 0 35 136 20 546.6 0 20 137 27 546.6 0 27 138 23 546.6 0 23 139 14 546.6 0 14 140 29 546.6 0 29 141 21 546.6 0 21 142 41 546.6 0 41 143 26 546.6 0 26 144 26 546.6 0 26 145 18 546.6 0 18 146 36 546.6 0 36 147 23 546.6 0 23 148 29 546.6 0 29 149 52 546.6 0 52 150 59 546.6 0 59 151 89 546.6 0 89 152 131 546.6 0 131 153 37 546.6 0 37 154 33 546.6 0 33 155 40 546.6 0 40 156 38 546.6 0 38 157 20 546.6 0 20 158 27 546.6 0 27 159 33 546.6 0 33 160 52 546.6 0 52 161 49 546.6 0 49 162 96 546.6 0 96 163 35 546.6 0 35 164 36 546.6 0 36 165 132 546.6 0 132 166 127 546.6 0 127 167 176 546.6 0 176 168 78 546.6 0 78 169 85 546.6 0 85 170 74 546.6 0 74 171 50 546.6 0 50 172 51 546.6 0 51 173 32 546.6 0 32 174 27 546.6 0 27 175 34 546.6 0 34 176 42 546.6 0 42 177 56 546.6 0 56 178 50 546.6 0 50 179 81 546.6 0 81 180 60 546.6 0 60 181 66 546.6 0 66 182 60 546.6 0 60 183 69 546.6 0 69 184 58 546.6 0 58 185 457 546.6 0 457 186 276 546.6 0 276 187 1595 546.6 0 1595 188 1440 546.6 0 1440 189 645 546.6 0 645 190 30 546.6 0 30 191 38 546.6 0 38 192 44 546.6 0 44 193 50 546.6 0 50 194 46 546.6 0 46 195 34 546.6 0 34 196 34 546.6 0 34 197 49 546.6 0 49 198 52 546.6 0 52 199 100 546.6 0 100 200 383 546.6 0 383 201 82 546.6 0 82 202 59 546.6 0 59 203 72 546.6 0 72 204 75 546.6 0 75 205 34 546.6 0 34 206 83 546.6 0 83 207 140 546.6 0 140 208 48 546.6 0 48 209 81 546.6 0 81 210 532 546.6 0 532 211 46 546.6 0 46 212 28 546.6 0 28 213 42 546.6 0 42 214 51 546.6 0 51 215 41 546.6 0 41 216 43 546.6 0 43 217 110 546.6 0 110 218 192 546.6 0 192 219 332 546.6 0 332 220 1061 546.6 0 1061 221 1604 546.6 0 1604 222 3581 546.6 0 3581 223 1853 546.6 0 1853 224 1229 546.6 0 1229 225 437 546.6 0 437 226 218 546.6 0 218 227 315 546.6 0 315 228 150 546.6 0 150 229 249 546.6 0 249 230 397 546.6 0 397 231 381 546.6 0 381 232 697 546.6 0 697 233 1298 546.6 0 1298 234 1653 546.6 0 1653 235 3882 546.6 0 3882 236 8461 546.6 0 8461 237 147741 546.6 0 147741 238 99254 546.6 0 99254 239 71388 546.6 0 71388 240 2495 546.6 0 2495 241 961 546.6 0 961 242 114 546.6 0 114 243 76 546.6 0 76 244 74 546.6 0 74 245 1092 546.6 0 1092 246 453 546.6 0 453 247 2764 546.6 0 2764 248 933 546.6 0 933 249 510 546.6 0 510 250 232 546.6 0 232 251 57 546.6 0 57 252 45 546.6 0 45 253 12 546.6 0 12 254 16 546.6 0 16 255 25 546.6 0 25 256 25 546.6 0 25 257 26 546.6 0 26 258 20 546.6 0 20 259 12 546.6 0 12 260 38 546.6 0 38 261 28 546.6 0 28 262 39 546.6 0 39 263 26 546.6 0 26 264 25 546.6 0 25 265 23 546.6 0 23 266 31 546.6 0 31 267 83 546.6 0 83 268 173 546.6 0 173 269 244 546.6 0 244 270 172 546.6 0 172 271 61 546.6 0 61 272 37 546.6 0 37 273 40 546.6 0 40 274 65 546.6 0 65 275 35 546.6 0 35 276 41 546.6 0 41 277 37 546.6 0 37 278 28 546.6 0 28 279 26 546.6 0 26 280 37 546.6 0 37 281 57 546.6 0 57 282 87 546.6 0 87 283 103 546.6 0 103 284 51 546.6 0 51 285 36 546.6 0 36 286 51 546.6 0 51 287 139 546.6 0 139 288 152 546.6 0 152 289 195 546.6 0 195 290 136 546.6 0 136 291 135 546.6 0 135 292 44 546.6 0 44 293 25 546.6 0 25 294 116 546.6 0 116 295 144 546.6 0 144 296 149 546.6 0 149 297 74 546.6 0 74 298 53 546.6 0 53 299 58 546.6 0 58 300 37 546.6 0 37 301 40 546.6 0 40 Finished in 3.24 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 65,712 Reads with adapters: 31,224 (47.5%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 31,218 (47.5%) Total basepairs processed: 17,660,104 bp Total written (filtered): 8,481,647 bp (48.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31224 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 4 19 179 0.0 3 0 177 2 20 30262 0.0 4 29736 506 18 1 1 21 75 0.0 4 8 67 22 9 0.0 4 5 0 3 0 1 23 16 0.0 4 0 0 1 14 1 24 1 0.0 4 0 0 0 0 1 28 2 0.0 4 2 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 657 0.0 4 0 0 0 0 657 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.05 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 31,218 Reads with adapters: 934 (3.0%) Reads written (passing filters): 31,218 (100.0%) Total basepairs processed: 8,481,647 bp Total written (filtered): 8,471,341 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 934 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.5% C: 3.3% G: 88.3% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 53 487.8 0 53 4 26 121.9 0 26 5 1 30.5 0 1 9 3 0.1 0 1 2 10 3 0.0 1 0 3 11 794 0.0 1 8 786 12 43 0.0 1 1 42 13 4 0.0 1 0 4 15 2 0.0 1 0 2 20 1 0.0 1 1 57 1 0.0 1 0 1 152 1 0.0 1 0 1 210 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.