This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.73 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 513,525 (22.9%) Reads that were too short: 62,666 (2.8%) Reads written (passing filters): 450,859 (20.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 101,430,575 bp (15.1%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 513525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5095 546.6 0 5095 7 54603 546.6 0 54603 8 1072 546.6 0 1072 9 68376 546.6 0 68376 10 441 546.6 0 441 11 34 546.6 0 34 12 34 546.6 0 34 13 30 546.6 0 30 14 50 546.6 0 50 15 53 546.6 0 53 16 73 546.6 0 73 17 67 546.6 0 67 18 59 546.6 0 59 19 82 546.6 0 82 20 84 546.6 0 84 21 49 546.6 0 49 22 66 546.6 0 66 23 49 546.6 0 49 24 57 546.6 0 57 25 52 546.6 0 52 26 39 546.6 0 39 27 47 546.6 0 47 28 44 546.6 0 44 29 60 546.6 0 60 30 42 546.6 0 42 31 52 546.6 0 52 32 36 546.6 0 36 33 38 546.6 0 38 34 70 546.6 0 70 35 89 546.6 0 89 36 93 546.6 0 93 37 60 546.6 0 60 38 64 546.6 0 64 39 47 546.6 0 47 40 54 546.6 0 54 41 110 546.6 0 110 42 129 546.6 0 129 43 92 546.6 0 92 44 66 546.6 0 66 45 107 546.6 0 107 46 96 546.6 0 96 47 168 546.6 0 168 48 109 546.6 0 109 49 251 546.6 0 251 50 1192 546.6 0 1192 51 606 546.6 0 606 52 70 546.6 0 70 53 122 546.6 0 122 54 107 546.6 0 107 55 107 546.6 0 107 56 67 546.6 0 67 57 75 546.6 0 75 58 120 546.6 0 120 59 203 546.6 0 203 60 1516 546.6 0 1516 61 1192 546.6 0 1192 62 2943 546.6 0 2943 63 139 546.6 0 139 64 66 546.6 0 66 65 122 546.6 0 122 66 87 546.6 0 87 67 190 546.6 0 190 68 236 546.6 0 236 69 329 546.6 0 329 70 499 546.6 0 499 71 977 546.6 0 977 72 1408 546.6 0 1408 73 661 546.6 0 661 74 494 546.6 0 494 75 1039 546.6 0 1039 76 430 546.6 0 430 77 383 546.6 0 383 78 727 546.6 0 727 79 726 546.6 0 726 80 964 546.6 0 964 81 1081 546.6 0 1081 82 1447 546.6 0 1447 83 2169 546.6 0 2169 84 2503 546.6 0 2503 85 45978 546.6 0 45978 86 55493 546.6 0 55493 87 119488 546.6 0 119488 88 471 546.6 0 471 89 43 546.6 0 43 90 49 546.6 0 49 91 55 546.6 0 55 92 59 546.6 0 59 93 43 546.6 0 43 94 49 546.6 0 49 95 42 546.6 0 42 96 55 546.6 0 55 97 37 546.6 0 37 98 43 546.6 0 43 99 48 546.6 0 48 100 42 546.6 0 42 101 28 546.6 0 28 102 41 546.6 0 41 103 51 546.6 0 51 104 24 546.6 0 24 105 31 546.6 0 31 106 44 546.6 0 44 107 58 546.6 0 58 108 56 546.6 0 56 109 51 546.6 0 51 110 39 546.6 0 39 111 38 546.6 0 38 112 29 546.6 0 29 113 34 546.6 0 34 114 45 546.6 0 45 115 41 546.6 0 41 116 44 546.6 0 44 117 43 546.6 0 43 118 47 546.6 0 47 119 39 546.6 0 39 120 54 546.6 0 54 121 77 546.6 0 77 122 74 546.6 0 74 123 130 546.6 0 130 124 138 546.6 0 138 125 61 546.6 0 61 126 51 546.6 0 51 127 57 546.6 0 57 128 69 546.6 0 69 129 103 546.6 0 103 130 182 546.6 0 182 131 495 546.6 0 495 132 772 546.6 0 772 133 109 546.6 0 109 134 102 546.6 0 102 135 155 546.6 0 155 136 110 546.6 0 110 137 103 546.6 0 103 138 81 546.6 0 81 139 69 546.6 0 69 140 91 546.6 0 91 141 88 546.6 0 88 142 86 546.6 0 86 143 109 546.6 0 109 144 86 546.6 0 86 145 86 546.6 0 86 146 107 546.6 0 107 147 89 546.6 0 89 148 141 546.6 0 141 149 217 546.6 0 217 150 356 546.6 0 356 151 434 546.6 0 434 152 407 546.6 0 407 153 2185 546.6 0 2185 154 2067 546.6 0 2067 155 2020 546.6 0 2020 156 352 546.6 0 352 157 102 546.6 0 102 158 69 546.6 0 69 159 91 546.6 0 91 160 91 546.6 0 91 161 140 546.6 0 140 162 230 546.6 0 230 163 89 546.6 0 89 164 135 546.6 0 135 165 195 546.6 0 195 166 113 546.6 0 113 167 115 546.6 0 115 168 308 546.6 0 308 169 218 546.6 0 218 170 273 546.6 0 273 171 343 546.6 0 343 172 447 546.6 0 447 173 767 546.6 0 767 174 1180 546.6 0 1180 175 8567 546.6 0 8567 176 24061 546.6 0 24061 177 19102 546.6 0 19102 178 130 546.6 0 130 179 53 546.6 0 53 180 80 546.6 0 80 181 137 546.6 0 137 182 101 546.6 0 101 183 156 546.6 0 156 184 80 546.6 0 80 185 81 546.6 0 81 186 102 546.6 0 102 187 111 546.6 0 111 188 55 546.6 0 55 189 108 546.6 0 108 190 75 546.6 0 75 191 107 546.6 0 107 192 77 546.6 0 77 193 59 546.6 0 59 194 80 546.6 0 80 195 64 546.6 0 64 196 84 546.6 0 84 197 66 546.6 0 66 198 80 546.6 0 80 199 71 546.6 0 71 200 69 546.6 0 69 201 120 546.6 0 120 202 71 546.6 0 71 203 87 546.6 0 87 204 329 546.6 0 329 205 140 546.6 0 140 206 157 546.6 0 157 207 91 546.6 0 91 208 152 546.6 0 152 209 193 546.6 0 193 210 354 546.6 0 354 211 515 546.6 0 515 212 1412 546.6 0 1412 213 22901 546.6 0 22901 214 1147 546.6 0 1147 215 11591 546.6 0 11591 216 208 546.6 0 208 217 193 546.6 0 193 218 3279 546.6 0 3279 219 98 546.6 0 98 220 102 546.6 0 102 221 112 546.6 0 112 222 154 546.6 0 154 223 44 546.6 0 44 224 67 546.6 0 67 225 80 546.6 0 80 226 85 546.6 0 85 227 94 546.6 0 94 228 189 546.6 0 189 229 178 546.6 0 178 230 219 546.6 0 219 231 198 546.6 0 198 232 362 546.6 0 362 233 253 546.6 0 253 234 211 546.6 0 211 235 119 546.6 0 119 236 77 546.6 0 77 237 97 546.6 0 97 238 99 546.6 0 99 239 84 546.6 0 84 240 97 546.6 0 97 241 87 546.6 0 87 242 66 546.6 0 66 243 76 546.6 0 76 244 61 546.6 0 61 245 78 546.6 0 78 246 99 546.6 0 99 247 227 546.6 0 227 248 247 546.6 0 247 249 297 546.6 0 297 250 219 546.6 0 219 251 184 546.6 0 184 252 183 546.6 0 183 253 1020 546.6 0 1020 254 722 546.6 0 722 255 1536 546.6 0 1536 256 417 546.6 0 417 257 220 546.6 0 220 258 294 546.6 0 294 259 155 546.6 0 155 260 88 546.6 0 88 261 94 546.6 0 94 262 78 546.6 0 78 263 64 546.6 0 64 264 62 546.6 0 62 265 52 546.6 0 52 266 86 546.6 0 86 267 92 546.6 0 92 268 124 546.6 0 124 269 142 546.6 0 142 270 105 546.6 0 105 271 136 546.6 0 136 272 96 546.6 0 96 273 68 546.6 0 68 274 88 546.6 0 88 275 129 546.6 0 129 276 113 546.6 0 113 277 212 546.6 0 212 278 307 546.6 0 307 279 432 546.6 0 432 280 353 546.6 0 353 281 163 546.6 0 163 282 145 546.6 0 145 283 155 546.6 0 155 284 109 546.6 0 109 285 876 546.6 0 876 286 305 546.6 0 305 287 828 546.6 0 828 288 177 546.6 0 177 289 208 546.6 0 208 290 256 546.6 0 256 291 138 546.6 0 138 292 160 546.6 0 160 293 333 546.6 0 333 294 361 546.6 0 361 295 336 546.6 0 336 296 354 546.6 0 354 297 856 546.6 0 856 298 755 546.6 0 755 299 589 546.6 0 589 300 359 546.6 0 359 301 255 546.6 0 255 Finished in 16.96 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 450,859 Reads with adapters: 81,278 (18.0%) Reads that were too short: 2,070 (0.5%) Reads written (passing filters): 79,208 (17.6%) Total basepairs processed: 101,430,575 bp Total written (filtered): 20,493,142 bp (20.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 81278 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 39 0.0 3 0 0 17 22 16 401 0.0 3 0 315 54 32 17 69695 0.0 3 64000 4037 757 901 18 201 0.0 3 12 152 16 21 19 369 0.0 3 219 9 76 65 20 289 0.0 3 1 3 3 282 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 0 2 23 3 0.0 3 0 0 0 3 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 1 0.0 3 1 30 1 0.0 3 1 32 1 0.0 3 0 0 1 33 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 8 0.0 3 0 0 0 8 37 1334 0.0 3 0 0 9 1325 38 144 0.0 3 0 0 1 143 39 9 0.0 3 0 0 0 9 40 1 0.0 3 0 0 0 1 41 11 0.0 3 0 0 0 11 43 3 0.0 3 0 0 1 2 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 6 0.0 3 0 0 0 6 52 1 0.0 3 0 0 0 1 54 9 0.0 3 0 0 0 9 56 4 0.0 3 0 0 0 4 58 3 0.0 3 0 0 0 3 60 2 0.0 3 0 0 0 2 62 4 0.0 3 0 0 0 4 64 20 0.0 3 0 0 0 20 65 66 0.0 3 0 0 1 65 66 7 0.0 3 0 0 0 7 67 9 0.0 3 0 0 0 9 68 20 0.0 3 0 0 0 20 69 4 0.0 3 0 0 1 3 70 3 0.0 3 0 0 1 2 72 2 0.0 3 0 0 0 2 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 23 0.0 3 0 0 0 23 80 1 0.0 3 0 0 0 1 81 26 0.0 3 0 0 0 26 82 10 0.0 3 1 0 0 9 83 6 0.0 3 0 0 0 6 84 4 0.0 3 0 0 0 4 85 8 0.0 3 0 0 0 8 86 2786 0.0 3 0 0 32 2754 87 505 0.0 3 0 0 8 497 88 848 0.0 3 0 0 12 836 89 1053 0.0 3 0 0 15 1038 90 137 0.0 3 0 0 4 133 91 177 0.0 3 0 0 1 176 92 73 0.0 3 0 0 3 70 93 13 0.0 3 0 0 0 13 94 41 0.0 3 0 0 0 41 95 5 0.0 3 0 0 0 5 96 7 0.0 3 0 0 0 7 97 10 0.0 3 0 0 0 10 98 15 0.0 3 0 0 0 15 99 19 0.0 3 0 0 0 19 100 17 0.0 3 0 0 1 16 101 29 0.0 3 0 0 0 29 102 16 0.0 3 0 0 0 16 103 12 0.0 3 0 0 1 11 104 6 0.0 3 0 0 0 6 105 13 0.0 3 0 0 0 13 106 78 0.0 3 0 0 1 77 107 164 0.0 3 0 0 4 160 108 4 0.0 3 0 0 1 3 109 16 0.0 3 0 0 1 15 110 49 0.0 3 0 0 4 45 111 4 0.0 3 0 0 0 4 112 89 0.0 3 0 0 1 88 113 23 0.0 3 0 0 1 22 114 21 0.0 3 0 0 2 19 115 13 0.0 3 0 0 0 13 116 3 0.0 3 0 0 0 3 117 5 0.0 3 0 0 0 5 118 14 0.0 3 0 0 0 14 119 8 0.0 3 0 0 0 8 120 5 0.0 3 0 0 0 5 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 13 0.0 3 0 0 0 13 125 2 0.0 3 0 0 1 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 5 0.0 3 0 0 0 5 131 156 0.0 3 0 0 4 152 132 17 0.0 3 0 0 0 17 133 6 0.0 3 0 0 1 5 134 26 0.0 3 0 0 1 25 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 45 0.0 3 0 0 0 45 138 24 0.0 3 0 0 0 24 139 37 0.0 3 0 0 0 37 140 15 0.0 3 0 0 1 14 141 9 0.0 3 0 0 0 9 142 6 0.0 3 0 0 0 6 143 36 0.0 3 0 0 0 36 145 1 0.0 3 0 0 0 1 146 8 0.0 3 0 0 0 8 147 4 0.0 3 0 0 0 4 148 6 0.0 3 0 0 0 6 149 2 0.0 3 0 0 0 2 150 5 0.0 3 0 0 0 5 151 1 0.0 3 0 0 0 1 152 5 0.0 3 0 0 0 5 153 3 0.0 3 0 0 0 3 154 2 0.0 3 0 0 0 2 156 5 0.0 3 0 0 0 5 157 12 0.0 3 0 0 1 11 158 24 0.0 3 0 0 0 24 159 2 0.0 3 0 0 0 2 160 4 0.0 3 0 0 0 4 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 6 0.0 3 0 0 0 6 164 1 0.0 3 0 0 0 1 166 4 0.0 3 0 0 0 4 167 5 0.0 3 0 0 1 4 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 1 170 2 0.0 3 0 0 0 2 171 2 0.0 3 0 0 1 1 172 3 0.0 3 0 0 0 3 173 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 178 4 0.0 3 0 0 0 4 179 5 0.0 3 0 0 0 5 180 1 0.0 3 0 0 0 1 181 5 0.0 3 0 0 0 5 182 10 0.0 3 0 0 0 10 183 8 0.0 3 0 0 0 8 184 7 0.0 3 0 0 1 6 185 2 0.0 3 0 0 0 2 186 7 0.0 3 0 0 0 7 187 7 0.0 3 0 0 0 7 188 22 0.0 3 0 0 2 20 189 10 0.0 3 0 0 1 9 190 5 0.0 3 0 0 0 5 191 5 0.0 3 0 0 0 5 192 3 0.0 3 0 0 0 3 193 7 0.0 3 0 0 0 7 194 10 0.0 3 0 0 2 8 195 2 0.0 3 0 0 0 2 197 11 0.0 3 0 0 0 11 198 319 0.0 3 0 0 0 319 199 134 0.0 3 0 0 6 128 200 183 0.0 3 0 0 6 177 201 215 0.0 3 0 0 7 208 202 122 0.0 3 0 0 8 114 203 118 0.0 3 0 0 9 109 204 81 0.0 3 0 1 3 77 205 18 0.0 3 0 0 0 18 206 64 0.0 3 0 0 2 62 207 51 0.0 3 0 0 2 49 208 31 0.0 3 0 0 2 29 209 10 0.0 3 0 0 0 10 210 5 0.0 3 0 0 0 5 211 5 0.0 3 0 0 0 5 212 6 0.0 3 0 0 0 6 213 6 0.0 3 0 0 0 6 214 8 0.0 3 0 0 1 7 215 16 0.0 3 0 0 0 16 216 63 0.0 3 0 0 0 63 217 13 0.0 3 0 0 0 13 218 3 0.0 3 0 0 0 3 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 1 1 223 4 0.0 3 0 0 1 3 226 14 0.0 3 0 0 0 14 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 7 0.0 3 0 0 0 7 233 3 0.0 3 0 0 0 3 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 270 2 0.0 3 0 0 0 2 277 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 279 11 0.0 3 0 0 0 11 280 10 0.0 3 0 0 0 10 281 14 0.0 3 0 0 0 14 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 1 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.67 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 79,208 Reads with adapters: 3,187 (4.0%) Reads written (passing filters): 79,208 (100.0%) Total basepairs processed: 20,493,142 bp Total written (filtered): 20,483,521 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3187 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.1% C: 29.7% G: 35.0% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3141 1237.6 0 3141 4 40 309.4 0 40 5 4 77.4 0 4 6 1 19.3 0 1 12 1 0.0 1 0 1