This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.97 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 44,010 (2.0%) Reads that were too short: 7,710 (0.3%) Reads written (passing filters): 36,300 (1.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,710,155 bp (1.4%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 44010 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 232 546.6 0 232 7 291 546.6 0 291 8 14915 546.6 0 14915 9 12678 546.6 0 12678 10 20 546.6 0 20 11 7 546.6 0 7 12 7 546.6 0 7 13 2 546.6 0 2 14 3 546.6 0 3 15 4 546.6 0 4 16 5 546.6 0 5 17 2 546.6 0 2 18 2 546.6 0 2 19 4 546.6 0 4 20 3 546.6 0 3 21 3 546.6 0 3 22 9 546.6 0 9 23 21 546.6 0 21 24 3 546.6 0 3 25 6 546.6 0 6 26 2 546.6 0 2 27 5 546.6 0 5 28 2 546.6 0 2 29 10 546.6 0 10 30 13 546.6 0 13 31 5 546.6 0 5 32 6 546.6 0 6 33 26 546.6 0 26 34 27 546.6 0 27 35 24 546.6 0 24 36 17 546.6 0 17 37 5 546.6 0 5 38 56 546.6 0 56 39 21 546.6 0 21 40 14 546.6 0 14 41 4 546.6 0 4 43 3 546.6 0 3 44 6 546.6 0 6 45 5 546.6 0 5 46 3 546.6 0 3 47 1 546.6 0 1 48 6 546.6 0 6 50 7 546.6 0 7 51 4 546.6 0 4 52 7 546.6 0 7 53 10 546.6 0 10 54 3 546.6 0 3 55 6 546.6 0 6 56 9 546.6 0 9 57 5 546.6 0 5 58 49 546.6 0 49 59 23 546.6 0 23 60 82 546.6 0 82 61 2 546.6 0 2 62 6 546.6 0 6 63 8 546.6 0 8 64 8 546.6 0 8 65 7 546.6 0 7 66 7 546.6 0 7 67 18 546.6 0 18 68 4 546.6 0 4 69 8 546.6 0 8 70 18 546.6 0 18 71 60 546.6 0 60 72 8 546.6 0 8 73 15 546.6 0 15 74 27 546.6 0 27 75 14 546.6 0 14 76 68 546.6 0 68 77 427 546.6 0 427 78 479 546.6 0 479 79 7 546.6 0 7 80 157 546.6 0 157 81 83 546.6 0 83 82 286 546.6 0 286 83 17 546.6 0 17 84 8 546.6 0 8 85 22 546.6 0 22 86 8 546.6 0 8 87 8 546.6 0 8 88 22 546.6 0 22 89 12 546.6 0 12 90 11 546.6 0 11 91 19 546.6 0 19 92 6 546.6 0 6 93 20 546.6 0 20 94 18 546.6 0 18 95 10 546.6 0 10 96 20 546.6 0 20 97 14 546.6 0 14 98 9 546.6 0 9 99 43 546.6 0 43 100 640 546.6 0 640 101 708 546.6 0 708 102 632 546.6 0 632 103 12 546.6 0 12 104 6 546.6 0 6 105 6 546.6 0 6 106 8 546.6 0 8 107 7 546.6 0 7 108 14 546.6 0 14 109 116 546.6 0 116 110 228 546.6 0 228 111 177 546.6 0 177 112 136 546.6 0 136 113 9 546.6 0 9 114 6 546.6 0 6 115 4 546.6 0 4 116 5 546.6 0 5 117 13 546.6 0 13 118 9 546.6 0 9 119 4 546.6 0 4 120 12 546.6 0 12 121 7 546.6 0 7 122 6 546.6 0 6 123 8 546.6 0 8 124 3 546.6 0 3 125 4 546.6 0 4 126 14 546.6 0 14 127 9 546.6 0 9 128 23 546.6 0 23 129 13 546.6 0 13 130 13 546.6 0 13 131 23 546.6 0 23 132 31 546.6 0 31 133 22 546.6 0 22 134 11 546.6 0 11 135 16 546.6 0 16 136 10 546.6 0 10 137 19 546.6 0 19 138 8 546.6 0 8 139 2 546.6 0 2 140 17 546.6 0 17 141 7 546.6 0 7 142 19 546.6 0 19 143 6 546.6 0 6 144 25 546.6 0 25 145 19 546.6 0 19 146 9 546.6 0 9 147 43 546.6 0 43 148 38 546.6 0 38 149 29 546.6 0 29 150 37 546.6 0 37 151 95 546.6 0 95 152 47 546.6 0 47 153 24 546.6 0 24 154 8 546.6 0 8 155 6 546.6 0 6 156 9 546.6 0 9 157 27 546.6 0 27 158 48 546.6 0 48 159 46 546.6 0 46 160 11 546.6 0 11 161 12 546.6 0 12 162 15 546.6 0 15 163 9 546.6 0 9 164 11 546.6 0 11 165 8 546.6 0 8 166 10 546.6 0 10 167 14 546.6 0 14 168 60 546.6 0 60 169 22 546.6 0 22 170 137 546.6 0 137 171 7 546.6 0 7 172 6 546.6 0 6 173 8 546.6 0 8 174 8 546.6 0 8 175 8 546.6 0 8 176 11 546.6 0 11 177 17 546.6 0 17 178 16 546.6 0 16 179 14 546.6 0 14 180 17 546.6 0 17 181 18 546.6 0 18 182 31 546.6 0 31 183 38 546.6 0 38 184 93 546.6 0 93 185 33 546.6 0 33 186 50 546.6 0 50 187 38 546.6 0 38 188 12 546.6 0 12 189 14 546.6 0 14 190 17 546.6 0 17 191 20 546.6 0 20 192 26 546.6 0 26 193 23 546.6 0 23 194 13 546.6 0 13 195 33 546.6 0 33 196 86 546.6 0 86 197 58 546.6 0 58 198 77 546.6 0 77 199 191 546.6 0 191 200 318 546.6 0 318 201 470 546.6 0 470 202 212 546.6 0 212 203 175 546.6 0 175 204 485 546.6 0 485 205 98 546.6 0 98 206 57 546.6 0 57 207 45 546.6 0 45 208 40 546.6 0 40 209 24 546.6 0 24 210 27 546.6 0 27 211 23 546.6 0 23 212 43 546.6 0 43 213 47 546.6 0 47 214 25 546.6 0 25 215 36 546.6 0 36 216 28 546.6 0 28 217 54 546.6 0 54 218 143 546.6 0 143 219 191 546.6 0 191 220 74 546.6 0 74 221 53 546.6 0 53 222 1039 546.6 0 1039 223 286 546.6 0 286 224 1142 546.6 0 1142 225 86 546.6 0 86 226 31 546.6 0 31 227 17 546.6 0 17 228 22 546.6 0 22 229 30 546.6 0 30 230 18 546.6 0 18 231 22 546.6 0 22 232 30 546.6 0 30 233 17 546.6 0 17 234 18 546.6 0 18 235 29 546.6 0 29 236 26 546.6 0 26 237 24 546.6 0 24 238 37 546.6 0 37 239 34 546.6 0 34 240 29 546.6 0 29 241 49 546.6 0 49 242 46 546.6 0 46 243 65 546.6 0 65 244 24 546.6 0 24 245 26 546.6 0 26 246 20 546.6 0 20 247 17 546.6 0 17 248 17 546.6 0 17 249 19 546.6 0 19 250 31 546.6 0 31 251 48 546.6 0 48 252 28 546.6 0 28 253 33 546.6 0 33 254 36 546.6 0 36 255 52 546.6 0 52 256 69 546.6 0 69 257 114 546.6 0 114 258 79 546.6 0 79 259 43 546.6 0 43 260 53 546.6 0 53 261 26 546.6 0 26 262 63 546.6 0 63 263 54 546.6 0 54 264 79 546.6 0 79 265 53 546.6 0 53 266 63 546.6 0 63 267 25 546.6 0 25 268 28 546.6 0 28 269 30 546.6 0 30 270 30 546.6 0 30 271 53 546.6 0 53 272 36 546.6 0 36 273 48 546.6 0 48 274 36 546.6 0 36 275 35 546.6 0 35 276 72 546.6 0 72 277 71 546.6 0 71 278 119 546.6 0 119 279 46 546.6 0 46 280 69 546.6 0 69 281 25 546.6 0 25 282 44 546.6 0 44 283 48 546.6 0 48 284 35 546.6 0 35 285 50 546.6 0 50 286 27 546.6 0 27 287 33 546.6 0 33 288 41 546.6 0 41 289 42 546.6 0 42 290 46 546.6 0 46 291 73 546.6 0 73 292 88 546.6 0 88 293 58 546.6 0 58 294 54 546.6 0 54 295 37 546.6 0 37 296 26 546.6 0 26 297 52 546.6 0 52 298 55 546.6 0 55 299 54 546.6 0 54 300 66 546.6 0 66 301 34 546.6 0 34 Finished in 1.79 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 36,300 Reads with adapters: 15,032 (41.4%) Reads that were too short: 37 (0.1%) Reads written (passing filters): 14,995 (41.3%) Total basepairs processed: 9,710,155 bp Total written (filtered): 4,131,744 bp (42.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15032 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 5 0.0 3 0 0 1 4 16 115 0.0 3 0 93 13 9 17 14656 0.0 3 13634 854 134 34 18 56 0.0 3 4 43 5 4 19 64 0.0 3 0 0 36 28 20 51 0.0 3 4 0 1 46 23 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 82 6 0.0 3 0 0 0 6 84 2 0.0 3 0 0 1 1 85 3 0.0 3 0 0 1 2 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 10 0.0 3 0 0 0 10 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 1 157 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 14,995 Reads with adapters: 120 (0.8%) Reads written (passing filters): 14,995 (100.0%) Total basepairs processed: 4,131,744 bp Total written (filtered): 4,131,348 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 120 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.0% C: 15.0% G: 31.7% T: 8.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 87 234.3 0 87 4 30 58.6 0 30 5 3 14.6 0 3