This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 36,076 (1.6%) Reads that were too short: 2,259 (0.1%) Reads written (passing filters): 33,817 (1.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,471,914 bp (1.4%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 36076 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 546.6 0 208 7 257 546.6 0 257 8 12552 546.6 0 12552 9 16507 546.6 0 16507 10 13 546.6 0 13 11 4 546.6 0 4 12 2 546.6 0 2 13 2 546.6 0 2 14 3 546.6 0 3 15 3 546.6 0 3 16 3 546.6 0 3 17 2 546.6 0 2 18 1 546.6 0 1 19 4 546.6 0 4 20 3 546.6 0 3 23 2 546.6 0 2 25 1 546.6 0 1 26 2 546.6 0 2 27 2 546.6 0 2 29 4 546.6 0 4 30 2 546.6 0 2 31 5 546.6 0 5 32 4 546.6 0 4 33 4 546.6 0 4 34 6 546.6 0 6 35 21 546.6 0 21 36 1 546.6 0 1 37 3 546.6 0 3 38 29 546.6 0 29 39 18 546.6 0 18 40 10 546.6 0 10 41 1 546.6 0 1 42 2 546.6 0 2 43 2 546.6 0 2 44 3 546.6 0 3 45 4 546.6 0 4 46 2 546.6 0 2 47 1 546.6 0 1 48 4 546.6 0 4 49 2 546.6 0 2 50 3 546.6 0 3 51 1 546.6 0 1 52 4 546.6 0 4 53 1 546.6 0 1 54 3 546.6 0 3 55 6 546.6 0 6 56 4 546.6 0 4 57 6 546.6 0 6 58 54 546.6 0 54 59 21 546.6 0 21 60 47 546.6 0 47 61 2 546.6 0 2 62 1 546.6 0 1 63 4 546.6 0 4 64 3 546.6 0 3 65 2 546.6 0 2 66 2 546.6 0 2 67 4 546.6 0 4 68 3 546.6 0 3 69 9 546.6 0 9 70 8 546.6 0 8 71 62 546.6 0 62 72 11 546.6 0 11 73 13 546.6 0 13 74 16 546.6 0 16 75 11 546.6 0 11 76 50 546.6 0 50 77 411 546.6 0 411 78 390 546.6 0 390 79 5 546.6 0 5 80 42 546.6 0 42 81 33 546.6 0 33 82 100 546.6 0 100 83 3 546.6 0 3 84 1 546.6 0 1 85 5 546.6 0 5 87 4 546.6 0 4 88 4 546.6 0 4 89 5 546.6 0 5 90 6 546.6 0 6 91 6 546.6 0 6 92 8 546.6 0 8 93 2 546.6 0 2 94 2 546.6 0 2 95 12 546.6 0 12 96 4 546.6 0 4 97 4 546.6 0 4 98 8 546.6 0 8 99 22 546.6 0 22 100 366 546.6 0 366 101 356 546.6 0 356 102 276 546.6 0 276 103 9 546.6 0 9 104 5 546.6 0 5 105 6 546.6 0 6 106 5 546.6 0 5 107 6 546.6 0 6 108 15 546.6 0 15 109 121 546.6 0 121 110 248 546.6 0 248 111 165 546.6 0 165 112 133 546.6 0 133 113 2 546.6 0 2 114 4 546.6 0 4 115 18 546.6 0 18 116 5 546.6 0 5 117 2 546.6 0 2 118 5 546.6 0 5 119 3 546.6 0 3 120 3 546.6 0 3 121 4 546.6 0 4 122 8 546.6 0 8 123 2 546.6 0 2 124 2 546.6 0 2 125 4 546.6 0 4 126 9 546.6 0 9 127 4 546.6 0 4 128 26 546.6 0 26 129 7 546.6 0 7 130 7 546.6 0 7 131 14 546.6 0 14 132 4 546.6 0 4 133 3 546.6 0 3 134 3 546.6 0 3 135 1 546.6 0 1 136 4 546.6 0 4 137 11 546.6 0 11 138 8 546.6 0 8 139 7 546.6 0 7 140 6 546.6 0 6 141 6 546.6 0 6 142 2 546.6 0 2 143 5 546.6 0 5 144 1 546.6 0 1 145 2 546.6 0 2 146 2 546.6 0 2 147 23 546.6 0 23 148 19 546.6 0 19 149 16 546.6 0 16 150 16 546.6 0 16 151 83 546.6 0 83 152 31 546.6 0 31 153 7 546.6 0 7 154 4 546.6 0 4 155 4 546.6 0 4 156 7 546.6 0 7 157 7 546.6 0 7 158 7 546.6 0 7 159 7 546.6 0 7 160 6 546.6 0 6 161 7 546.6 0 7 162 6 546.6 0 6 163 6 546.6 0 6 164 3 546.6 0 3 165 2 546.6 0 2 166 2 546.6 0 2 167 7 546.6 0 7 168 50 546.6 0 50 169 10 546.6 0 10 170 129 546.6 0 129 171 5 546.6 0 5 172 4 546.6 0 4 174 2 546.6 0 2 176 3 546.6 0 3 177 9 546.6 0 9 178 6 546.6 0 6 179 8 546.6 0 8 180 6 546.6 0 6 181 5 546.6 0 5 182 6 546.6 0 6 183 8 546.6 0 8 184 9 546.6 0 9 185 7 546.6 0 7 186 12 546.6 0 12 187 3 546.6 0 3 188 5 546.6 0 5 189 2 546.6 0 2 190 2 546.6 0 2 191 6 546.6 0 6 192 1 546.6 0 1 193 8 546.6 0 8 194 8 546.6 0 8 195 6 546.6 0 6 196 7 546.6 0 7 197 16 546.6 0 16 198 14 546.6 0 14 199 41 546.6 0 41 200 48 546.6 0 48 201 69 546.6 0 69 202 27 546.6 0 27 203 19 546.6 0 19 204 77 546.6 0 77 205 22 546.6 0 22 206 15 546.6 0 15 207 12 546.6 0 12 208 13 546.6 0 13 209 13 546.6 0 13 210 5 546.6 0 5 211 5 546.6 0 5 212 11 546.6 0 11 213 14 546.6 0 14 214 10 546.6 0 10 215 10 546.6 0 10 216 5 546.6 0 5 217 21 546.6 0 21 218 47 546.6 0 47 219 53 546.6 0 53 220 12 546.6 0 12 221 13 546.6 0 13 222 205 546.6 0 205 223 66 546.6 0 66 224 250 546.6 0 250 225 23 546.6 0 23 226 7 546.6 0 7 227 7 546.6 0 7 228 7 546.6 0 7 229 13 546.6 0 13 230 6 546.6 0 6 231 8 546.6 0 8 232 9 546.6 0 9 233 12 546.6 0 12 234 7 546.6 0 7 235 10 546.6 0 10 236 15 546.6 0 15 237 10 546.6 0 10 238 17 546.6 0 17 239 16 546.6 0 16 240 27 546.6 0 27 241 16 546.6 0 16 242 17 546.6 0 17 243 15 546.6 0 15 244 16 546.6 0 16 245 11 546.6 0 11 246 8 546.6 0 8 247 10 546.6 0 10 248 13 546.6 0 13 249 11 546.6 0 11 250 10 546.6 0 10 251 30 546.6 0 30 252 7 546.6 0 7 253 11 546.6 0 11 254 10 546.6 0 10 255 19 546.6 0 19 256 28 546.6 0 28 257 38 546.6 0 38 258 23 546.6 0 23 259 17 546.6 0 17 260 16 546.6 0 16 261 11 546.6 0 11 262 11 546.6 0 11 263 28 546.6 0 28 264 29 546.6 0 29 265 21 546.6 0 21 266 19 546.6 0 19 267 9 546.6 0 9 268 13 546.6 0 13 269 15 546.6 0 15 270 11 546.6 0 11 271 19 546.6 0 19 272 15 546.6 0 15 273 24 546.6 0 24 274 10 546.6 0 10 275 13 546.6 0 13 276 25 546.6 0 25 277 16 546.6 0 16 278 30 546.6 0 30 279 10 546.6 0 10 280 9 546.6 0 9 281 15 546.6 0 15 282 14 546.6 0 14 283 18 546.6 0 18 284 28 546.6 0 28 285 21 546.6 0 21 286 21 546.6 0 21 287 18 546.6 0 18 288 33 546.6 0 33 289 29 546.6 0 29 290 20 546.6 0 20 291 31 546.6 0 31 292 42 546.6 0 42 293 19 546.6 0 19 294 21 546.6 0 21 295 12 546.6 0 12 296 18 546.6 0 18 297 21 546.6 0 21 298 24 546.6 0 24 299 27 546.6 0 27 300 21 546.6 0 21 301 23 546.6 0 23 Finished in 1.75 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 33,817 Reads with adapters: 16,986 (50.2%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 16,984 (50.2%) Total basepairs processed: 9,471,914 bp Total written (filtered): 4,620,191 bp (48.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16986 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 3 0 1 19 64 0.0 3 0 62 1 0 1 20 16857 0.0 4 16530 317 9 1 21 43 0.0 4 9 34 22 1 0.0 4 0 0 1 23 7 0.0 4 0 0 0 4 3 24 5 0.0 4 5 76 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.59 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 16,984 Reads with adapters: 2,248 (13.2%) Reads written (passing filters): 16,984 (100.0%) Total basepairs processed: 4,620,191 bp Total written (filtered): 4,596,375 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2248 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.7% G: 94.0% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 265.4 0 2 4 3 66.3 0 3 6 1 4.1 0 1 8 1 0.3 0 1 9 994 0.1 0 22 972 10 1095 0.0 1 42 1053 11 135 0.0 1 1 134 12 4 0.0 1 0 4 13 1 0.0 1 0 1 138 1 0.0 1 1 150 3 0.0 1 0 3 208 2 0.0 1 0 2 223 6 0.0 1 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.