This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.17 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 303,425 (10.7%) Reads that were too short: 5,705 (0.2%) Reads written (passing filters): 297,720 (10.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 63,542,330 bp (7.4%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 303425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1452 692.0 0 1452 7 61724 692.0 0 61724 8 57572 692.0 0 57572 9 41 692.0 0 41 10 11 692.0 0 11 11 75 692.0 0 75 12 54 692.0 0 54 13 185 692.0 0 185 14 10 692.0 0 10 15 7 692.0 0 7 16 8 692.0 0 8 17 11 692.0 0 11 18 10 692.0 0 10 19 12 692.0 0 12 20 11 692.0 0 11 21 15 692.0 0 15 22 11 692.0 0 11 23 6 692.0 0 6 24 15 692.0 0 15 25 27 692.0 0 27 26 13 692.0 0 13 27 23 692.0 0 23 28 15 692.0 0 15 29 4 692.0 0 4 30 5 692.0 0 5 31 26 692.0 0 26 32 11 692.0 0 11 33 18 692.0 0 18 34 24 692.0 0 24 35 10 692.0 0 10 36 14 692.0 0 14 37 29 692.0 0 29 38 37 692.0 0 37 39 11 692.0 0 11 40 24 692.0 0 24 41 12 692.0 0 12 42 14 692.0 0 14 43 22 692.0 0 22 44 20 692.0 0 20 45 41 692.0 0 41 46 4 692.0 0 4 47 21 692.0 0 21 48 7 692.0 0 7 49 2 692.0 0 2 50 9 692.0 0 9 51 23 692.0 0 23 52 7 692.0 0 7 53 10 692.0 0 10 54 11 692.0 0 11 55 7 692.0 0 7 56 18 692.0 0 18 57 6 692.0 0 6 58 14 692.0 0 14 59 20 692.0 0 20 60 20 692.0 0 20 61 10 692.0 0 10 62 5 692.0 0 5 63 10 692.0 0 10 64 7 692.0 0 7 65 8 692.0 0 8 66 17 692.0 0 17 67 11 692.0 0 11 68 6 692.0 0 6 69 13 692.0 0 13 70 19 692.0 0 19 71 28 692.0 0 28 72 100 692.0 0 100 73 34 692.0 0 34 74 272 692.0 0 272 75 38 692.0 0 38 76 10 692.0 0 10 77 11 692.0 0 11 78 20 692.0 0 20 79 13 692.0 0 13 80 34 692.0 0 34 81 19 692.0 0 19 82 19 692.0 0 19 83 19 692.0 0 19 84 17 692.0 0 17 85 15 692.0 0 15 86 12 692.0 0 12 87 70 692.0 0 70 88 14 692.0 0 14 89 30 692.0 0 30 90 28 692.0 0 28 91 35 692.0 0 35 92 22 692.0 0 22 93 35 692.0 0 35 94 24 692.0 0 24 95 12 692.0 0 12 96 48 692.0 0 48 97 49 692.0 0 49 98 69 692.0 0 69 99 86 692.0 0 86 100 48 692.0 0 48 101 13 692.0 0 13 102 75 692.0 0 75 103 48 692.0 0 48 104 56 692.0 0 56 105 106 692.0 0 106 106 53 692.0 0 53 107 89 692.0 0 89 108 81 692.0 0 81 109 43 692.0 0 43 110 69 692.0 0 69 111 56 692.0 0 56 112 126 692.0 0 126 113 69 692.0 0 69 114 50 692.0 0 50 115 381 692.0 0 381 116 39 692.0 0 39 117 23 692.0 0 23 118 43 692.0 0 43 119 36 692.0 0 36 120 29 692.0 0 29 121 51 692.0 0 51 122 256 692.0 0 256 123 134 692.0 0 134 124 310 692.0 0 310 125 497 692.0 0 497 126 138 692.0 0 138 127 279 692.0 0 279 128 134 692.0 0 134 129 149 692.0 0 149 130 295 692.0 0 295 131 153 692.0 0 153 132 159 692.0 0 159 133 105 692.0 0 105 134 153 692.0 0 153 135 280 692.0 0 280 136 346 692.0 0 346 137 584 692.0 0 584 138 1006 692.0 0 1006 139 1132 692.0 0 1132 140 2511 692.0 0 2511 141 4081 692.0 0 4081 142 79232 692.0 0 79232 143 22338 692.0 0 22338 144 48289 692.0 0 48289 145 223 692.0 0 223 146 57 692.0 0 57 147 76 692.0 0 76 148 55 692.0 0 55 149 56 692.0 0 56 150 45 692.0 0 45 151 52 692.0 0 52 152 35 692.0 0 35 153 31 692.0 0 31 154 32 692.0 0 32 155 50 692.0 0 50 156 46 692.0 0 46 157 66 692.0 0 66 158 61 692.0 0 61 159 76 692.0 0 76 160 174 692.0 0 174 161 309 692.0 0 309 162 5068 692.0 0 5068 163 941 692.0 0 941 164 1643 692.0 0 1643 165 30 692.0 0 30 166 21 692.0 0 21 167 46 692.0 0 46 168 37 692.0 0 37 169 47 692.0 0 47 170 38 692.0 0 38 171 20 692.0 0 20 172 18 692.0 0 18 173 12 692.0 0 12 174 11 692.0 0 11 175 34 692.0 0 34 176 49 692.0 0 49 177 92 692.0 0 92 178 45 692.0 0 45 179 28 692.0 0 28 180 19 692.0 0 19 181 24 692.0 0 24 182 18 692.0 0 18 183 29 692.0 0 29 184 32 692.0 0 32 185 26 692.0 0 26 186 20 692.0 0 20 187 29 692.0 0 29 188 221 692.0 0 221 189 51 692.0 0 51 190 120 692.0 0 120 191 15 692.0 0 15 192 9 692.0 0 9 193 22 692.0 0 22 194 25 692.0 0 25 195 25 692.0 0 25 196 29 692.0 0 29 197 34 692.0 0 34 198 30 692.0 0 30 199 32 692.0 0 32 200 20 692.0 0 20 201 23 692.0 0 23 202 35 692.0 0 35 203 31 692.0 0 31 204 31 692.0 0 31 205 29 692.0 0 29 206 35 692.0 0 35 207 37 692.0 0 37 208 36 692.0 0 36 209 47 692.0 0 47 210 52 692.0 0 52 211 49 692.0 0 49 212 59 692.0 0 59 213 53 692.0 0 53 214 265 692.0 0 265 215 170 692.0 0 170 216 77 692.0 0 77 217 43 692.0 0 43 218 35 692.0 0 35 219 25 692.0 0 25 220 23 692.0 0 23 221 30 692.0 0 30 222 63 692.0 0 63 223 63 692.0 0 63 224 75 692.0 0 75 225 89 692.0 0 89 226 69 692.0 0 69 227 34 692.0 0 34 228 11 692.0 0 11 229 22 692.0 0 22 230 24 692.0 0 24 231 23 692.0 0 23 232 116 692.0 0 116 233 71 692.0 0 71 234 164 692.0 0 164 235 33 692.0 0 33 236 29 692.0 0 29 237 35 692.0 0 35 238 44 692.0 0 44 239 38 692.0 0 38 240 40 692.0 0 40 241 79 692.0 0 79 242 36 692.0 0 36 243 31 692.0 0 31 244 26 692.0 0 26 245 45 692.0 0 45 246 35 692.0 0 35 247 41 692.0 0 41 248 24 692.0 0 24 249 26 692.0 0 26 250 30 692.0 0 30 251 30 692.0 0 30 252 32 692.0 0 32 253 34 692.0 0 34 254 33 692.0 0 33 255 47 692.0 0 47 256 54 692.0 0 54 257 65 692.0 0 65 258 50 692.0 0 50 259 49 692.0 0 49 260 55 692.0 0 55 261 59 692.0 0 59 262 110 692.0 0 110 263 175 692.0 0 175 264 139 692.0 0 139 265 42 692.0 0 42 266 35 692.0 0 35 267 28 692.0 0 28 268 46 692.0 0 46 269 56 692.0 0 56 270 106 692.0 0 106 271 59 692.0 0 59 272 31 692.0 0 31 273 42 692.0 0 42 274 53 692.0 0 53 275 59 692.0 0 59 276 119 692.0 0 119 277 46 692.0 0 46 278 37 692.0 0 37 279 70 692.0 0 70 280 79 692.0 0 79 281 219 692.0 0 219 282 67 692.0 0 67 283 95 692.0 0 95 284 60 692.0 0 60 285 62 692.0 0 62 286 45 692.0 0 45 287 50 692.0 0 50 288 37 692.0 0 37 289 32 692.0 0 32 290 40 692.0 0 40 291 40 692.0 0 40 292 62 692.0 0 62 293 31 692.0 0 31 294 52 692.0 0 52 295 67 692.0 0 67 296 56 692.0 0 56 297 105 692.0 0 105 298 57 692.0 0 57 299 26 692.0 0 26 300 42 692.0 0 42 301 42 692.0 0 42 Finished in 11.25 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 297,720 Reads with adapters: 61,596 (20.7%) Reads that were too short: 136 (0.0%) Reads written (passing filters): 61,460 (20.6%) Total basepairs processed: 63,542,330 bp Total written (filtered): 17,019,579 bp (26.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 61596 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 52 0.0 3 0 0 24 28 16 901 0.0 3 0 792 70 39 17 59978 0.0 3 55740 3487 613 138 18 170 0.0 3 8 108 19 35 19 157 0.0 3 3 1 75 78 20 162 0.0 3 0 1 0 161 25 1 0.0 3 1 29 2 0.0 3 0 0 0 2 40 2 0.0 3 1 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 9 0.0 3 0 0 0 9 60 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 77 1 0.0 3 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 96 1 0.0 3 1 102 1 0.0 3 0 0 0 1 103 4 0.0 3 0 0 0 4 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 144 5 0.0 3 0 0 0 5 145 2 0.0 3 0 0 0 2 146 11 0.0 3 0 0 0 11 147 13 0.0 3 0 0 0 13 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 155 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 180 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 11 0.0 3 0 0 0 11 195 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 279 7 0.0 3 0 0 0 7 280 3 0.0 3 0 0 0 3 281 8 0.0 3 0 0 0 8 282 5 0.0 3 0 0 0 5 283 2 0.0 3 0 0 0 2 285 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.17 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 61,460 Reads with adapters: 987 (1.6%) Reads written (passing filters): 61,460 (100.0%) Total basepairs processed: 17,019,579 bp Total written (filtered): 17,016,571 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 987 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.2% C: 71.1% G: 8.9% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 957 960.3 0 957 4 14 240.1 0 14 5 15 60.0 0 15 6 1 15.0 0 1