This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.45 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 320,033 (11.3%) Reads that were too short: 4,315 (0.2%) Reads written (passing filters): 315,718 (11.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 66,857,941 bp (7.8%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 320033 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1127 692.0 0 1127 7 56150 692.0 0 56150 8 67265 692.0 0 67265 9 43 692.0 0 43 10 9 692.0 0 9 11 33 692.0 0 33 12 37 692.0 0 37 13 70 692.0 0 70 14 6 692.0 0 6 15 6 692.0 0 6 16 6 692.0 0 6 17 3 692.0 0 3 18 7 692.0 0 7 19 12 692.0 0 12 20 16 692.0 0 16 21 16 692.0 0 16 22 13 692.0 0 13 23 3 692.0 0 3 24 7 692.0 0 7 25 4 692.0 0 4 26 8 692.0 0 8 27 17 692.0 0 17 28 14 692.0 0 14 29 2 692.0 0 2 30 3 692.0 0 3 31 10 692.0 0 10 32 4 692.0 0 4 33 9 692.0 0 9 34 27 692.0 0 27 35 10 692.0 0 10 36 4 692.0 0 4 37 9 692.0 0 9 38 4 692.0 0 4 39 11 692.0 0 11 40 7 692.0 0 7 41 7 692.0 0 7 42 13 692.0 0 13 43 27 692.0 0 27 44 21 692.0 0 21 45 44 692.0 0 44 46 9 692.0 0 9 47 16 692.0 0 16 48 2 692.0 0 2 49 4 692.0 0 4 50 8 692.0 0 8 51 30 692.0 0 30 52 6 692.0 0 6 53 5 692.0 0 5 54 16 692.0 0 16 55 13 692.0 0 13 56 9 692.0 0 9 57 11 692.0 0 11 58 30 692.0 0 30 59 16 692.0 0 16 60 14 692.0 0 14 61 7 692.0 0 7 62 9 692.0 0 9 63 2 692.0 0 2 64 4 692.0 0 4 65 11 692.0 0 11 66 12 692.0 0 12 67 9 692.0 0 9 68 2 692.0 0 2 69 10 692.0 0 10 70 20 692.0 0 20 71 35 692.0 0 35 72 92 692.0 0 92 73 19 692.0 0 19 74 278 692.0 0 278 75 18 692.0 0 18 76 3 692.0 0 3 77 13 692.0 0 13 78 20 692.0 0 20 79 10 692.0 0 10 80 13 692.0 0 13 81 15 692.0 0 15 82 26 692.0 0 26 83 18 692.0 0 18 84 19 692.0 0 19 85 22 692.0 0 22 86 13 692.0 0 13 87 60 692.0 0 60 88 22 692.0 0 22 89 63 692.0 0 63 90 34 692.0 0 34 91 69 692.0 0 69 92 46 692.0 0 46 93 67 692.0 0 67 94 17 692.0 0 17 95 21 692.0 0 21 96 26 692.0 0 26 97 27 692.0 0 27 98 60 692.0 0 60 99 49 692.0 0 49 100 29 692.0 0 29 101 27 692.0 0 27 102 64 692.0 0 64 103 40 692.0 0 40 104 45 692.0 0 45 105 124 692.0 0 124 106 82 692.0 0 82 107 83 692.0 0 83 108 82 692.0 0 82 109 64 692.0 0 64 110 51 692.0 0 51 111 57 692.0 0 57 112 109 692.0 0 109 113 54 692.0 0 54 114 78 692.0 0 78 115 394 692.0 0 394 116 43 692.0 0 43 117 22 692.0 0 22 118 27 692.0 0 27 119 24 692.0 0 24 120 47 692.0 0 47 121 37 692.0 0 37 122 277 692.0 0 277 123 106 692.0 0 106 124 306 692.0 0 306 125 548 692.0 0 548 126 153 692.0 0 153 127 276 692.0 0 276 128 144 692.0 0 144 129 170 692.0 0 170 130 326 692.0 0 326 131 150 692.0 0 150 132 150 692.0 0 150 133 116 692.0 0 116 134 185 692.0 0 185 135 283 692.0 0 283 136 298 692.0 0 298 137 540 692.0 0 540 138 978 692.0 0 978 139 1167 692.0 0 1167 140 2494 692.0 0 2494 141 3997 692.0 0 3997 142 86149 692.0 0 86149 143 24304 692.0 0 24304 144 54304 692.0 0 54304 145 230 692.0 0 230 146 39 692.0 0 39 147 66 692.0 0 66 148 34 692.0 0 34 149 49 692.0 0 49 150 47 692.0 0 47 151 31 692.0 0 31 152 27 692.0 0 27 153 37 692.0 0 37 154 22 692.0 0 22 155 69 692.0 0 69 156 38 692.0 0 38 157 67 692.0 0 67 158 63 692.0 0 63 159 74 692.0 0 74 160 139 692.0 0 139 161 315 692.0 0 315 162 4938 692.0 0 4938 163 930 692.0 0 930 164 1716 692.0 0 1716 165 20 692.0 0 20 166 15 692.0 0 15 167 42 692.0 0 42 168 36 692.0 0 36 169 37 692.0 0 37 170 22 692.0 0 22 171 14 692.0 0 14 172 14 692.0 0 14 173 16 692.0 0 16 174 8 692.0 0 8 175 21 692.0 0 21 176 46 692.0 0 46 177 71 692.0 0 71 178 43 692.0 0 43 179 29 692.0 0 29 180 5 692.0 0 5 181 15 692.0 0 15 182 17 692.0 0 17 183 19 692.0 0 19 184 23 692.0 0 23 185 27 692.0 0 27 186 24 692.0 0 24 187 27 692.0 0 27 188 223 692.0 0 223 189 47 692.0 0 47 190 147 692.0 0 147 191 13 692.0 0 13 192 11 692.0 0 11 193 22 692.0 0 22 194 17 692.0 0 17 195 37 692.0 0 37 196 32 692.0 0 32 197 32 692.0 0 32 198 22 692.0 0 22 199 22 692.0 0 22 200 16 692.0 0 16 201 17 692.0 0 17 202 30 692.0 0 30 203 25 692.0 0 25 204 26 692.0 0 26 205 22 692.0 0 22 206 37 692.0 0 37 207 37 692.0 0 37 208 30 692.0 0 30 209 38 692.0 0 38 210 43 692.0 0 43 211 30 692.0 0 30 212 33 692.0 0 33 213 32 692.0 0 32 214 158 692.0 0 158 215 69 692.0 0 69 216 62 692.0 0 62 217 32 692.0 0 32 218 22 692.0 0 22 219 17 692.0 0 17 220 19 692.0 0 19 221 28 692.0 0 28 222 51 692.0 0 51 223 44 692.0 0 44 224 37 692.0 0 37 225 81 692.0 0 81 226 95 692.0 0 95 227 41 692.0 0 41 228 21 692.0 0 21 229 45 692.0 0 45 230 19 692.0 0 19 231 21 692.0 0 21 232 60 692.0 0 60 233 37 692.0 0 37 234 62 692.0 0 62 235 40 692.0 0 40 236 35 692.0 0 35 237 30 692.0 0 30 238 32 692.0 0 32 239 32 692.0 0 32 240 36 692.0 0 36 241 71 692.0 0 71 242 39 692.0 0 39 243 33 692.0 0 33 244 26 692.0 0 26 245 35 692.0 0 35 246 31 692.0 0 31 247 24 692.0 0 24 248 18 692.0 0 18 249 17 692.0 0 17 250 27 692.0 0 27 251 37 692.0 0 37 252 33 692.0 0 33 253 22 692.0 0 22 254 22 692.0 0 22 255 41 692.0 0 41 256 42 692.0 0 42 257 72 692.0 0 72 258 44 692.0 0 44 259 50 692.0 0 50 260 56 692.0 0 56 261 49 692.0 0 49 262 71 692.0 0 71 263 135 692.0 0 135 264 59 692.0 0 59 265 31 692.0 0 31 266 27 692.0 0 27 267 24 692.0 0 24 268 28 692.0 0 28 269 39 692.0 0 39 270 65 692.0 0 65 271 39 692.0 0 39 272 27 692.0 0 27 273 35 692.0 0 35 274 25 692.0 0 25 275 44 692.0 0 44 276 56 692.0 0 56 277 40 692.0 0 40 278 27 692.0 0 27 279 53 692.0 0 53 280 60 692.0 0 60 281 192 692.0 0 192 282 67 692.0 0 67 283 57 692.0 0 57 284 43 692.0 0 43 285 32 692.0 0 32 286 31 692.0 0 31 287 33 692.0 0 33 288 23 692.0 0 23 289 32 692.0 0 32 290 36 692.0 0 36 291 24 692.0 0 24 292 48 692.0 0 48 293 31 692.0 0 31 294 49 692.0 0 49 295 51 692.0 0 51 296 57 692.0 0 57 297 100 692.0 0 100 298 37 692.0 0 37 299 26 692.0 0 26 300 43 692.0 0 43 301 20 692.0 0 20 Finished in 12.91 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 315,718 Reads with adapters: 68,996 (21.9%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 68,967 (21.8%) Total basepairs processed: 66,857,941 bp Total written (filtered): 18,821,023 bp (28.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 68996 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 4 1 19 186 0.0 3 0 176 9 1 20 68483 0.0 4 67264 1170 47 2 21 159 0.0 4 24 133 1 0 1 22 9 0.0 4 2 0 7 23 48 0.0 4 0 0 0 43 5 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 31 2 0.0 4 2 37 6 0.0 4 0 0 0 0 6 38 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 114 1 0.0 4 0 0 0 0 1 122 1 0.0 4 1 127 54 0.0 4 0 0 0 0 54 129 1 0.0 4 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 1 237 2 0.0 4 1 0 0 0 1 242 1 0.0 4 1 265 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.40 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 68,967 Reads with adapters: 6,043 (8.8%) Reads written (passing filters): 68,967 (100.0%) Total basepairs processed: 18,821,023 bp Total written (filtered): 18,755,334 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6043 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 3.1% G: 93.5% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 41 1077.6 0 41 4 19 269.4 0 19 5 3 67.4 0 3 6 2 16.8 0 2 7 1 4.2 0 1 8 2 1.1 0 2 9 176 0.3 0 7 169 10 1008 0.1 1 46 962 11 4765 0.0 1 42 4723 12 10 0.0 1 0 10 13 3 0.0 1 0 3 14 4 0.0 1 0 4 15 2 0.0 1 0 2 54 1 0.0 1 1 151 3 0.0 1 0 3 191 1 0.0 1 0 1 209 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.