This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.35 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 369,174 (13.0%) Reads that were too short: 23,815 (0.8%) Reads written (passing filters): 345,359 (12.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 97,095,867 bp (11.4%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 369174 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1994 692.0 0 1994 7 57957 692.0 0 57957 8 2462 692.0 0 2462 9 45028 692.0 0 45028 10 1928 692.0 0 1928 11 2134 692.0 0 2134 12 38584 692.0 0 38584 13 44478 692.0 0 44478 14 126572 692.0 0 126572 15 220 692.0 0 220 16 42 692.0 0 42 17 35 692.0 0 35 18 34 692.0 0 34 19 33 692.0 0 33 20 21 692.0 0 21 21 29 692.0 0 29 22 26 692.0 0 26 23 23 692.0 0 23 24 37 692.0 0 37 25 29 692.0 0 29 26 72 692.0 0 72 27 120 692.0 0 120 28 51 692.0 0 51 29 38 692.0 0 38 30 31 692.0 0 31 31 30 692.0 0 30 32 34 692.0 0 34 33 40 692.0 0 40 34 38 692.0 0 38 35 24 692.0 0 24 36 32 692.0 0 32 37 16 692.0 0 16 38 35 692.0 0 35 39 35 692.0 0 35 40 43 692.0 0 43 41 32 692.0 0 32 42 27 692.0 0 27 43 84 692.0 0 84 44 42 692.0 0 42 45 51 692.0 0 51 46 34 692.0 0 34 47 23 692.0 0 23 48 25 692.0 0 25 49 37 692.0 0 37 50 35 692.0 0 35 51 38 692.0 0 38 52 30 692.0 0 30 53 32 692.0 0 32 54 21 692.0 0 21 55 26 692.0 0 26 56 34 692.0 0 34 57 30 692.0 0 30 58 47 692.0 0 47 59 28 692.0 0 28 60 23 692.0 0 23 61 22 692.0 0 22 62 23 692.0 0 23 63 38 692.0 0 38 64 31 692.0 0 31 65 46 692.0 0 46 66 36 692.0 0 36 67 44 692.0 0 44 68 27 692.0 0 27 69 17 692.0 0 17 70 43 692.0 0 43 71 31 692.0 0 31 72 38 692.0 0 38 73 91 692.0 0 91 74 50 692.0 0 50 75 35 692.0 0 35 76 41 692.0 0 41 77 31 692.0 0 31 78 50 692.0 0 50 79 55 692.0 0 55 80 37 692.0 0 37 81 38 692.0 0 38 82 36 692.0 0 36 83 41 692.0 0 41 84 47 692.0 0 47 85 39 692.0 0 39 86 27 692.0 0 27 87 46 692.0 0 46 88 39 692.0 0 39 89 45 692.0 0 45 90 55 692.0 0 55 91 41 692.0 0 41 92 104 692.0 0 104 93 44 692.0 0 44 94 68 692.0 0 68 95 66 692.0 0 66 96 61 692.0 0 61 97 43 692.0 0 43 98 65 692.0 0 65 99 46 692.0 0 46 100 39 692.0 0 39 101 35 692.0 0 35 102 57 692.0 0 57 103 33 692.0 0 33 104 211 692.0 0 211 105 155 692.0 0 155 106 523 692.0 0 523 107 98 692.0 0 98 108 179 692.0 0 179 109 86 692.0 0 86 110 139 692.0 0 139 111 104 692.0 0 104 112 112 692.0 0 112 113 95 692.0 0 95 114 94 692.0 0 94 115 70 692.0 0 70 116 67 692.0 0 67 117 63 692.0 0 63 118 63 692.0 0 63 119 83 692.0 0 83 120 75 692.0 0 75 121 76 692.0 0 76 122 75 692.0 0 75 123 111 692.0 0 111 124 1031 692.0 0 1031 125 764 692.0 0 764 126 3085 692.0 0 3085 127 450 692.0 0 450 128 461 692.0 0 461 129 204 692.0 0 204 130 64 692.0 0 64 131 76 692.0 0 76 132 43 692.0 0 43 133 72 692.0 0 72 134 80 692.0 0 80 135 127 692.0 0 127 136 87 692.0 0 87 137 116 692.0 0 116 138 708 692.0 0 708 139 335 692.0 0 335 140 1245 692.0 0 1245 141 140 692.0 0 140 142 231 692.0 0 231 143 63 692.0 0 63 144 67 692.0 0 67 145 102 692.0 0 102 146 158 692.0 0 158 147 107 692.0 0 107 148 166 692.0 0 166 149 53 692.0 0 53 150 77 692.0 0 77 151 407 692.0 0 407 152 137 692.0 0 137 153 251 692.0 0 251 154 77 692.0 0 77 155 83 692.0 0 83 156 90 692.0 0 90 157 150 692.0 0 150 158 756 692.0 0 756 159 444 692.0 0 444 160 538 692.0 0 538 161 1280 692.0 0 1280 162 140 692.0 0 140 163 169 692.0 0 169 164 58 692.0 0 58 165 71 692.0 0 71 166 50 692.0 0 50 167 86 692.0 0 86 168 66 692.0 0 66 169 118 692.0 0 118 170 46 692.0 0 46 171 61 692.0 0 61 172 44 692.0 0 44 173 73 692.0 0 73 174 100 692.0 0 100 175 83 692.0 0 83 176 76 692.0 0 76 177 82 692.0 0 82 178 42 692.0 0 42 179 79 692.0 0 79 180 62 692.0 0 62 181 114 692.0 0 114 182 142 692.0 0 142 183 158 692.0 0 158 184 82 692.0 0 82 185 65 692.0 0 65 186 79 692.0 0 79 187 62 692.0 0 62 188 253 692.0 0 253 189 188 692.0 0 188 190 210 692.0 0 210 191 45 692.0 0 45 192 58 692.0 0 58 193 59 692.0 0 59 194 69 692.0 0 69 195 65 692.0 0 65 196 56 692.0 0 56 197 69 692.0 0 69 198 80 692.0 0 80 199 108 692.0 0 108 200 77 692.0 0 77 201 105 692.0 0 105 202 87 692.0 0 87 203 70 692.0 0 70 204 57 692.0 0 57 205 58 692.0 0 58 206 57 692.0 0 57 207 114 692.0 0 114 208 81 692.0 0 81 209 125 692.0 0 125 210 70 692.0 0 70 211 68 692.0 0 68 212 72 692.0 0 72 213 103 692.0 0 103 214 234 692.0 0 234 215 144 692.0 0 144 216 133 692.0 0 133 217 83 692.0 0 83 218 94 692.0 0 94 219 101 692.0 0 101 220 353 692.0 0 353 221 451 692.0 0 451 222 216 692.0 0 216 223 106 692.0 0 106 224 370 692.0 0 370 225 84 692.0 0 84 226 465 692.0 0 465 227 73 692.0 0 73 228 56 692.0 0 56 229 53 692.0 0 53 230 113 692.0 0 113 231 172 692.0 0 172 232 198 692.0 0 198 233 478 692.0 0 478 234 317 692.0 0 317 235 137 692.0 0 137 236 102 692.0 0 102 237 64 692.0 0 64 238 57 692.0 0 57 239 47 692.0 0 47 240 90 692.0 0 90 241 98 692.0 0 98 242 158 692.0 0 158 243 105 692.0 0 105 244 107 692.0 0 107 245 73 692.0 0 73 246 52 692.0 0 52 247 65 692.0 0 65 248 58 692.0 0 58 249 57 692.0 0 57 250 65 692.0 0 65 251 108 692.0 0 108 252 90 692.0 0 90 253 145 692.0 0 145 254 115 692.0 0 115 255 65 692.0 0 65 256 68 692.0 0 68 257 92 692.0 0 92 258 89 692.0 0 89 259 57 692.0 0 57 260 90 692.0 0 90 261 112 692.0 0 112 262 123 692.0 0 123 263 128 692.0 0 128 264 93 692.0 0 93 265 86 692.0 0 86 266 104 692.0 0 104 267 158 692.0 0 158 268 192 692.0 0 192 269 359 692.0 0 359 270 135 692.0 0 135 271 116 692.0 0 116 272 74 692.0 0 74 273 67 692.0 0 67 274 103 692.0 0 103 275 102 692.0 0 102 276 166 692.0 0 166 277 630 692.0 0 630 278 334 692.0 0 334 279 396 692.0 0 396 280 947 692.0 0 947 281 4039 692.0 0 4039 282 2554 692.0 0 2554 283 2433 692.0 0 2433 284 539 692.0 0 539 285 102 692.0 0 102 286 80 692.0 0 80 287 93 692.0 0 93 288 206 692.0 0 206 289 231 692.0 0 231 290 536 692.0 0 536 291 88 692.0 0 88 292 117 692.0 0 117 293 77 692.0 0 77 294 122 692.0 0 122 295 131 692.0 0 131 296 176 692.0 0 176 297 89 692.0 0 89 298 69 692.0 0 69 299 49 692.0 0 49 300 63 692.0 0 63 301 86 692.0 0 86 Finished in 15.49 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 345,359 Reads with adapters: 63,103 (18.3%) Reads that were too short: 1,081 (0.3%) Reads written (passing filters): 62,022 (18.0%) Total basepairs processed: 97,095,867 bp Total written (filtered): 16,377,656 bp (16.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63103 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 30 0.0 3 0 0 21 9 16 436 0.0 3 0 368 41 27 17 55988 0.0 3 52282 3130 465 111 18 142 0.0 3 5 103 11 23 19 169 0.0 3 0 1 88 80 20 89 0.0 3 33 3 1 52 21 2 0.0 3 2 23 2 0.0 3 1 0 0 1 24 2 0.0 3 1 0 0 1 25 1 0.0 3 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 50 0.0 3 0 1 0 49 33 2 0.0 3 0 1 0 1 35 5 0.0 3 0 0 0 5 36 1 0.0 3 1 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 5 0.0 3 0 0 0 5 43 36 0.0 3 0 0 0 36 44 1 0.0 3 0 0 0 1 45 8 0.0 3 0 0 0 8 47 2 0.0 3 0 0 0 2 48 4 0.0 3 0 0 1 3 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 72 0.0 3 0 0 0 72 54 2 0.0 3 0 0 0 2 56 8 0.0 3 0 0 0 8 57 5 0.0 3 0 0 0 5 58 6 0.0 3 0 0 0 6 61 1 0.0 3 0 0 0 1 62 1 0.0 3 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 1 66 1 0.0 3 0 0 0 1 67 151 0.0 3 0 0 2 149 69 1 0.0 3 0 0 0 1 70 30 0.0 3 0 0 2 28 71 7 0.0 3 0 0 1 6 72 2 0.0 3 0 0 0 2 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 1 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 24 0.0 3 0 0 0 24 89 4 0.0 3 0 0 0 4 90 2 0.0 3 0 0 0 2 91 3 0.0 3 0 0 1 2 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 1 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 3 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 6 0.0 3 0 0 0 6 110 152 0.0 3 0 0 2 150 111 22 0.0 3 1 0 0 21 112 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 118 6 0.0 3 0 0 1 5 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 124 41 0.0 3 0 0 0 41 126 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 131 3 0.0 3 0 0 0 3 132 4 0.0 3 0 0 0 4 133 3 0.0 3 0 0 1 2 134 3 0.0 3 0 0 1 2 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 2 0.0 3 1 0 0 1 146 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 4 0.0 3 0 0 0 4 153 4 0.0 3 0 0 0 4 154 108 0.0 3 0 0 1 107 155 19 0.0 3 0 1 0 18 156 5 0.0 3 0 0 0 5 157 34 0.0 3 0 0 1 33 158 11 0.0 3 0 0 0 11 159 961 0.0 3 0 0 14 947 160 227 0.0 3 0 0 1 226 161 151 0.0 3 0 0 1 150 162 137 0.0 3 0 0 2 135 163 45 0.0 3 0 0 1 44 164 34 0.0 3 0 0 1 33 165 106 0.0 3 0 0 0 106 166 3 0.0 3 0 0 0 3 167 66 0.0 3 0 0 2 64 168 53 0.0 3 0 0 1 52 169 3 0.0 3 0 0 0 3 170 7 0.0 3 1 0 0 6 171 20 0.0 3 0 0 1 19 172 7 0.0 3 0 0 1 6 173 53 0.0 3 0 0 0 53 174 39 0.0 3 0 0 0 39 175 32 0.0 3 0 0 1 31 176 58 0.0 3 0 0 5 53 177 23 0.0 3 0 0 0 23 178 584 0.0 3 0 0 9 575 179 923 0.0 3 0 0 10 913 180 494 0.0 3 0 0 5 489 181 15 0.0 3 0 0 1 14 182 146 0.0 3 0 0 3 143 183 54 0.0 3 0 0 3 51 184 4 0.0 3 0 0 0 4 185 30 0.0 3 0 0 0 30 186 7 0.0 3 0 0 0 7 187 8 0.0 3 0 0 0 8 188 7 0.0 3 0 0 1 6 189 2 0.0 3 0 0 0 2 191 2 0.0 3 0 0 0 2 193 4 0.0 3 0 0 1 3 194 3 0.0 3 0 0 0 3 195 3 0.0 3 0 0 0 3 196 1 0.0 3 0 0 1 197 4 0.0 3 0 0 0 4 198 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 4 0.0 3 0 0 0 4 202 3 0.0 3 0 0 0 3 203 10 0.0 3 0 0 0 10 204 7 0.0 3 0 0 0 7 205 24 0.0 3 0 0 0 24 206 13 0.0 3 0 0 0 13 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 27 0.0 3 0 0 1 26 211 10 0.0 3 0 0 1 9 212 11 0.0 3 0 0 0 11 213 1 0.0 3 0 0 0 1 214 39 0.0 3 0 0 2 37 216 4 0.0 3 0 0 1 3 217 2 0.0 3 0 0 0 2 218 4 0.0 3 0 0 0 4 219 1 0.0 3 0 0 0 1 220 9 0.0 3 0 0 1 8 221 6 0.0 3 0 0 0 6 223 3 0.0 3 0 0 0 3 224 2 0.0 3 0 0 0 2 225 3 0.0 3 0 0 0 3 226 4 0.0 3 0 0 0 4 227 3 0.0 3 0 0 0 3 228 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 230 21 0.0 3 0 0 2 19 231 6 0.0 3 0 0 0 6 232 4 0.0 3 0 1 0 3 233 4 0.0 3 0 0 0 4 234 3 0.0 3 0 0 0 3 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 34 0.0 3 0 0 0 34 240 8 0.0 3 0 0 0 8 242 12 0.0 3 0 0 0 12 243 2 0.0 3 0 0 0 2 247 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 249 2 0.0 3 0 0 0 2 251 1 0.0 3 0 0 0 1 252 2 0.0 3 0 0 0 2 253 4 0.0 3 0 0 1 3 254 5 0.0 3 0 0 0 5 255 11 0.0 3 0 0 1 10 256 5 0.0 3 0 0 0 5 257 5 0.0 3 0 0 1 4 258 3 0.0 3 0 0 0 3 259 3 0.0 3 0 0 0 3 260 7 0.0 3 0 0 0 7 261 6 0.0 3 0 0 1 5 262 7 0.0 3 0 0 0 7 263 5 0.0 3 0 0 1 4 264 13 0.0 3 0 0 0 13 265 3 0.0 3 0 0 0 3 266 3 0.0 3 0 0 0 3 267 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 269 6 0.0 3 0 0 0 6 270 1 0.0 3 0 0 0 1 271 46 0.0 3 0 0 0 46 272 48 0.0 3 0 0 1 47 273 28 0.0 3 0 0 0 28 274 29 0.0 3 0 0 2 27 275 63 0.0 3 0 0 3 60 276 14 0.0 3 0 0 1 13 277 19 0.0 3 0 0 0 19 278 4 0.0 3 0 0 0 4 279 32 0.0 3 0 0 0 32 280 92 0.0 3 0 0 2 90 281 110 0.0 3 0 0 4 106 282 20 0.0 3 0 0 1 19 283 5 0.0 3 0 0 0 5 284 8 0.0 3 0 0 0 8 285 9 0.0 3 0 0 0 9 286 4 0.0 3 0 0 0 4 287 16 0.0 3 0 0 1 15 288 21 0.0 3 0 0 1 20 289 16 0.0 3 0 0 0 16 290 1 0.0 3 0 0 0 1 291 2 0.0 3 0 0 0 2 293 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 62,022 Reads with adapters: 1,461 (2.4%) Reads written (passing filters): 62,022 (100.0%) Total basepairs processed: 16,377,656 bp Total written (filtered): 16,373,247 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1461 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.0% C: 72.1% G: 9.1% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1447 969.1 0 1447 4 9 242.3 0 9 5 1 60.6 0 1 6 3 15.1 0 3 9 1 0.2 0 0 1