This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.32 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 343,279 (12.1%) Reads that were too short: 13,618 (0.5%) Reads written (passing filters): 329,661 (11.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 93,755,552 bp (11.0%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 343279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1819 692.0 0 1819 7 47941 692.0 0 47941 8 1338 692.0 0 1338 9 59100 692.0 0 59100 10 1498 692.0 0 1498 11 1492 692.0 0 1492 12 36176 692.0 0 36176 13 42799 692.0 0 42799 14 123094 692.0 0 123094 15 138 692.0 0 138 16 29 692.0 0 29 17 14 692.0 0 14 18 19 692.0 0 19 19 15 692.0 0 15 20 18 692.0 0 18 21 16 692.0 0 16 22 26 692.0 0 26 23 16 692.0 0 16 24 15 692.0 0 15 25 20 692.0 0 20 26 19 692.0 0 19 27 17 692.0 0 17 28 18 692.0 0 18 29 18 692.0 0 18 30 22 692.0 0 22 31 21 692.0 0 21 32 15 692.0 0 15 33 19 692.0 0 19 34 15 692.0 0 15 35 17 692.0 0 17 36 192 692.0 0 192 37 23 692.0 0 23 38 37 692.0 0 37 39 17 692.0 0 17 40 35 692.0 0 35 41 26 692.0 0 26 42 25 692.0 0 25 43 59 692.0 0 59 44 38 692.0 0 38 45 38 692.0 0 38 46 15 692.0 0 15 47 18 692.0 0 18 48 19 692.0 0 19 49 30 692.0 0 30 50 22 692.0 0 22 51 32 692.0 0 32 52 25 692.0 0 25 53 29 692.0 0 29 54 15 692.0 0 15 55 19 692.0 0 19 56 20 692.0 0 20 57 25 692.0 0 25 58 22 692.0 0 22 59 25 692.0 0 25 60 16 692.0 0 16 61 31 692.0 0 31 62 15 692.0 0 15 63 29 692.0 0 29 64 32 692.0 0 32 65 36 692.0 0 36 66 29 692.0 0 29 67 24 692.0 0 24 68 28 692.0 0 28 69 19 692.0 0 19 70 27 692.0 0 27 71 29 692.0 0 29 72 12 692.0 0 12 73 58 692.0 0 58 74 34 692.0 0 34 75 27 692.0 0 27 76 30 692.0 0 30 77 30 692.0 0 30 78 48 692.0 0 48 79 44 692.0 0 44 80 42 692.0 0 42 81 36 692.0 0 36 82 34 692.0 0 34 83 28 692.0 0 28 84 42 692.0 0 42 85 43 692.0 0 43 86 37 692.0 0 37 87 55 692.0 0 55 88 44 692.0 0 44 89 61 692.0 0 61 90 53 692.0 0 53 91 60 692.0 0 60 92 92 692.0 0 92 93 43 692.0 0 43 94 58 692.0 0 58 95 70 692.0 0 70 96 52 692.0 0 52 97 48 692.0 0 48 98 56 692.0 0 56 99 28 692.0 0 28 100 34 692.0 0 34 101 36 692.0 0 36 102 35 692.0 0 35 103 44 692.0 0 44 104 160 692.0 0 160 105 131 692.0 0 131 106 315 692.0 0 315 107 52 692.0 0 52 108 105 692.0 0 105 109 60 692.0 0 60 110 78 692.0 0 78 111 56 692.0 0 56 112 62 692.0 0 62 113 55 692.0 0 55 114 52 692.0 0 52 115 41 692.0 0 41 116 53 692.0 0 53 117 46 692.0 0 46 118 44 692.0 0 44 119 41 692.0 0 41 120 75 692.0 0 75 121 95 692.0 0 95 122 74 692.0 0 74 123 47 692.0 0 47 124 392 692.0 0 392 125 313 692.0 0 313 126 1281 692.0 0 1281 127 413 692.0 0 413 128 408 692.0 0 408 129 174 692.0 0 174 130 55 692.0 0 55 131 43 692.0 0 43 132 52 692.0 0 52 133 52 692.0 0 52 134 60 692.0 0 60 135 105 692.0 0 105 136 74 692.0 0 74 137 62 692.0 0 62 138 172 692.0 0 172 139 191 692.0 0 191 140 295 692.0 0 295 141 100 692.0 0 100 142 158 692.0 0 158 143 45 692.0 0 45 144 44 692.0 0 44 145 77 692.0 0 77 146 96 692.0 0 96 147 61 692.0 0 61 148 85 692.0 0 85 149 40 692.0 0 40 150 64 692.0 0 64 151 285 692.0 0 285 152 108 692.0 0 108 153 144 692.0 0 144 154 58 692.0 0 58 155 68 692.0 0 68 156 70 692.0 0 70 157 103 692.0 0 103 158 337 692.0 0 337 159 292 692.0 0 292 160 285 692.0 0 285 161 639 692.0 0 639 162 78 692.0 0 78 163 83 692.0 0 83 164 66 692.0 0 66 165 52 692.0 0 52 166 51 692.0 0 51 167 56 692.0 0 56 168 41 692.0 0 41 169 89 692.0 0 89 170 33 692.0 0 33 171 43 692.0 0 43 172 51 692.0 0 51 173 46 692.0 0 46 174 73 692.0 0 73 175 67 692.0 0 67 176 77 692.0 0 77 177 43 692.0 0 43 178 23 692.0 0 23 179 50 692.0 0 50 180 52 692.0 0 52 181 67 692.0 0 67 182 77 692.0 0 77 183 55 692.0 0 55 184 51 692.0 0 51 185 42 692.0 0 42 186 52 692.0 0 52 187 62 692.0 0 62 188 142 692.0 0 142 189 119 692.0 0 119 190 99 692.0 0 99 191 39 692.0 0 39 192 52 692.0 0 52 193 59 692.0 0 59 194 54 692.0 0 54 195 42 692.0 0 42 196 30 692.0 0 30 197 60 692.0 0 60 198 53 692.0 0 53 199 75 692.0 0 75 200 69 692.0 0 69 201 46 692.0 0 46 202 72 692.0 0 72 203 63 692.0 0 63 204 49 692.0 0 49 205 41 692.0 0 41 206 46 692.0 0 46 207 74 692.0 0 74 208 75 692.0 0 75 209 66 692.0 0 66 210 33 692.0 0 33 211 44 692.0 0 44 212 55 692.0 0 55 213 59 692.0 0 59 214 153 692.0 0 153 215 87 692.0 0 87 216 128 692.0 0 128 217 39 692.0 0 39 218 58 692.0 0 58 219 58 692.0 0 58 220 195 692.0 0 195 221 286 692.0 0 286 222 176 692.0 0 176 223 77 692.0 0 77 224 412 692.0 0 412 225 88 692.0 0 88 226 465 692.0 0 465 227 44 692.0 0 44 228 47 692.0 0 47 229 49 692.0 0 49 230 77 692.0 0 77 231 88 692.0 0 88 232 90 692.0 0 90 233 225 692.0 0 225 234 241 692.0 0 241 235 135 692.0 0 135 236 61 692.0 0 61 237 64 692.0 0 64 238 51 692.0 0 51 239 44 692.0 0 44 240 71 692.0 0 71 241 87 692.0 0 87 242 125 692.0 0 125 243 70 692.0 0 70 244 55 692.0 0 55 245 45 692.0 0 45 246 45 692.0 0 45 247 60 692.0 0 60 248 46 692.0 0 46 249 42 692.0 0 42 250 62 692.0 0 62 251 77 692.0 0 77 252 75 692.0 0 75 253 81 692.0 0 81 254 59 692.0 0 59 255 39 692.0 0 39 256 58 692.0 0 58 257 49 692.0 0 49 258 62 692.0 0 62 259 39 692.0 0 39 260 77 692.0 0 77 261 89 692.0 0 89 262 98 692.0 0 98 263 129 692.0 0 129 264 85 692.0 0 85 265 50 692.0 0 50 266 87 692.0 0 87 267 131 692.0 0 131 268 103 692.0 0 103 269 258 692.0 0 258 270 88 692.0 0 88 271 76 692.0 0 76 272 83 692.0 0 83 273 59 692.0 0 59 274 73 692.0 0 73 275 88 692.0 0 88 276 137 692.0 0 137 277 683 692.0 0 683 278 328 692.0 0 328 279 356 692.0 0 356 280 317 692.0 0 317 281 1307 692.0 0 1307 282 766 692.0 0 766 283 831 692.0 0 831 284 193 692.0 0 193 285 51 692.0 0 51 286 65 692.0 0 65 287 55 692.0 0 55 288 171 692.0 0 171 289 171 692.0 0 171 290 380 692.0 0 380 291 84 692.0 0 84 292 87 692.0 0 87 293 65 692.0 0 65 294 99 692.0 0 99 295 111 692.0 0 111 296 105 692.0 0 105 297 68 692.0 0 68 298 42 692.0 0 42 299 54 692.0 0 54 300 45 692.0 0 45 301 111 692.0 0 111 Finished in 18.27 s (55 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 329,661 Reads with adapters: 60,760 (18.4%) Reads that were too short: 76 (0.0%) Reads written (passing filters): 60,684 (18.4%) Total basepairs processed: 93,755,552 bp Total written (filtered): 16,413,823 bp (17.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 60760 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 137 0.0 3 0 128 7 2 20 59497 0.0 4 58391 1067 35 3 1 21 112 0.0 4 15 96 1 22 11 0.0 4 2 0 9 23 30 0.0 4 0 1 0 25 4 24 4 0.0 4 0 0 0 0 4 41 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 1 108 1 0.0 4 1 109 2 0.0 4 0 0 0 0 2 117 2 0.0 4 1 0 0 0 1 118 1 0.0 4 1 121 6 0.0 4 0 0 0 0 6 122 752 0.0 4 0 0 0 0 752 123 8 0.0 4 0 0 0 0 8 124 2 0.0 4 0 0 0 1 1 126 2 0.0 4 0 0 0 0 2 127 97 0.0 4 0 0 0 0 97 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 1 136 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 147 2 0.0 4 1 0 0 0 1 148 3 0.0 4 0 0 0 0 3 161 1 0.0 4 0 0 0 0 1 164 1 0.0 4 1 179 1 0.0 4 1 203 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 213 2 0.0 4 1 0 0 0 1 216 1 0.0 4 0 0 0 0 1 219 3 0.0 4 0 0 0 0 3 222 1 0.0 4 0 0 0 0 1 223 16 0.0 4 0 0 0 0 16 224 4 0.0 4 0 0 0 0 4 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 229 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 231 2 0.0 4 0 0 0 1 1 232 9 0.0 4 0 0 0 0 9 237 2 0.0 4 1 0 0 0 1 252 2 0.0 4 0 0 0 1 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 272 2 0.0 4 0 0 0 0 2 274 2 0.0 4 0 0 0 0 2 277 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.20 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 60,684 Reads with adapters: 8,627 (14.2%) Reads written (passing filters): 60,684 (100.0%) Total basepairs processed: 16,413,823 bp Total written (filtered): 16,330,025 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8627 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 2.9% G: 94.4% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 948.2 0 15 4 4 237.0 0 4 5 3 59.3 0 3 6 2 14.8 0 2 7 3 3.7 0 3 9 3214 0.2 0 102 3112 10 5054 0.1 1 173 4881 11 316 0.0 1 4 312 12 9 0.0 1 0 9 13 3 0.0 1 0 3 150 4 0.0 1 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.